Genetic structure in Atlantic brown trout (Salmo trutta L.) populations in the Iberian peninsula: evidence from mitochondrial and nuclear DNA analysis     

S. Dunner  L. Royo  J. Cañon 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2000,117(3):105-120

Brown trout (Salmo trutta L.) was sampled in rivers belonging to three different Spanish basins in order to analyse the distribution of genetic variability. The genetic analysis was performed by using two systems and techniques: nuclear DNA was screened through random amplified polymorphic DNAs (screening 2 × 10⁵ bp of the whole genome), and mitochondrial DNA (mtDNA) through sequencing of the hypervariable control region. Genetic distances between the populations were similar using either analysis although some differences arise. For example, some populations of the Tajo basin were very close through nuclear analysis but more distant using mtDNA. Differences between the two DNA sources could be the result of a different evolutionary rate, and the fact that mtDNA is maternally transmitted and differences in sex migration rates will influence the patterns of genetic variation between the transmitted DNAs. Total variation was partitioned using amova showing a clear subdivision among basins although intrapopulation variation remained as high as 62%. A correspondence analysis defined the differences in a three‐dimensional way, clustering the populations according to their common basin. When mtDNA was sequenced, higher variability was noted in the segment between 400 and 600bp of the whole D‐loop sequence, suggesting that these 200bp improved the analysis of the variability more than sequencing the t‐RNA ends of the control region. A comparison was made between the t‐RNA^Pro ends of the 10 populations screened here and the rest of the published sequences found in the literature, leading to a concentration of these populations in group IV which includes all trouts which originate in the Atlantic. The analyses performed suggest that a high genetic variability is present in all populations and that although there has been a probable interference from stocked strains introduced to increase population density, this was only detectable through the variance between rivers which reflect different policies according to the region where the basin is located. However, the genetic analysis using the two approaches allows the control of the natural populations avoiding a loss of their genetic potential.  相似文献   

Old and modern durum wheat varieties from Italy and Spain differ in main spike components     

Fanny Álvaro  Julio Isidro  Dolors Villegas  Luis F. García del Moral  Conxita Royo 《Field Crops Research》2008

Four field experiments comparing 24 durum wheat varieties grown at different periods during the 20th century in Italy and Spain were carried out to assess the changes caused by breeding activities on the number of grains per main spike and its determinants: number of spikelets per spike, number of grains per spikelet, fertile flowering and grain setting. Increases of 0.14 grains spike⁻¹ year⁻¹ (0.43% year⁻¹ in relative terms) and 0.08 grains spike⁻¹ year⁻¹ (0.22% year⁻¹) were observed in Italian and Spanish varieties, respectively. The overall change in the number of grains per spike in Italian germplasm (29.5%) was due to increases in both, the number of spikelets per spike (7.5%) and the number of grains per spikelet (20.3%), while in Spanish varieties the increase in the number of grains per spike (19.5%) was only attributed to the improvement of the number of grains per spikelet. The increase in the number of fertile florets per spike (about 12%) was similar in both countries, but while it explained more than 70% of the changes in the number of grains per spike in Spanish varieties, grain setting was responsible for most of the improvement in the number of grains per spike in the Italian germplasm. The percentage of florets setting grains was 68 and 64% in modern Italian and Spanish varieties, respectively. Most of the changes in the number of grains per spikelet were found in the upper part of the spike on Italian varieties, whilst they were more evenly distributed in the Spanish ones. The main achievement derived from the introduction of the Rht-B1 dwarfing gene was an increase in the number of grains per spikelet, but it did not have any effect on the number of spikelets on the main spike. The lack of genetic associations between grain setting and both the number of spikelets per spike and the number of fertile florets per spike suggests that future yield gains may be obtained through increases in the three components independently.  相似文献   

Natural Products from Antarctic Colonial Ascidians of the Genera Aplidium and Synoicum: Variability and Defensive Role     

Laura Nú?ez-Pons  Marianna Carbone  Jennifer Vázquez  Jaime Rodríguez  Rosa María Nieto  María Mercedes Varela  Margherita Gavagnin  Conxita Avila 《Marine drugs》2012,10(8):1741-1764

Ascidians have developed multiple defensive strategies mostly related to physical, nutritional or chemical properties of the tunic. One of such is chemical defense based on secondary metabolites. We analyzed a series of colonial Antarctic ascidians from deep-water collections belonging to the genera Aplidium and Synoicum to evaluate the incidence of organic deterrents and their variability. The ether fractions from 15 samples including specimens of the species A. falklandicum, A. fuegiense, A. meridianum, A. millari and S. adareanum were subjected to feeding assays towards two relevant sympatric predators: the starfish Odontaster validus, and the amphipod Cheirimedon femoratus. All samples revealed repellency. Nonetheless, some colonies concentrated defensive chemicals in internal body-regions rather than in the tunic. Four ascidian-derived meroterpenoids, rossinones B and the three derivatives 2,3-epoxy-rossinone B, 3-epi-rossinone B, 5,6-epoxy-rossinone B, and the indole alkaloids meridianins A–G, along with other minoritary meridianin compounds were isolated from several samples. Some purified metabolites were tested in feeding assays exhibiting potent unpalatabilities, thus revealing their role in predation avoidance. Ascidian extracts and purified compound-fractions were further assessed in antibacterial tests against a marine Antarctic bacterium. Only the meridianins showed inhibition activity, demonstrating a multifunctional defensive role. According to their occurrence in nature and within our colonial specimens, the possible origin of both types of metabolites is discussed.  相似文献   

Relationships among adaptation patterns, morphophysiological traits and molecular markers in durum wheat     

P. Annicchiarico    C. Royo    F. Bellah    M. Moragues 《Plant Breeding》2009,128(2):164-171

Breeding for specific or wide adaptation would be more cost-efficient if preliminary indications on genotype adaptive responses could be provided by easily-detectable morphophysiological or molecular characteristics. We investigated the consistency among adaptation parameters, morphophysiological traits and SSR markers in estimating and structuring the genetic diversity among 24 durum wheat genotypes. Additive main effects and multiplicative interaction adaptation parameters were estimated across 14 Algerian locations belonging to a low- or a high-elevation subregion. Eight morphophysiological traits were assessed in two locations per subregion. Genotype Euclidean distances based on adaptation parameters were closely related to those based on morphophysiological traits ( r  = 0.75, P = 0.01). Marker-based distances exhibited low correlation with those based on adaptation parameters ( r  = 0.26, P = 0.01) or morphophysiological traits ( r  = 0.32). The consistency between adaptation parameters and morphophysiological traits for genotype ordination and classification was greater than that between either information layer and SSR markers. Optima of phenology and plant height differed between subregions, whereas many kernels per spike and fertile tillers were associated with adaptation to both subregions.  相似文献   

Durum Wheat under Mediterranean Conditions as Affected by Seed Size     

C. Royo  A. Ramdani  M. Moragues  D. Villegas 《Journal of Agronomy and Crop Science》2006,192(4):257-266

This study was carried out in order to determine the effect of seed size on the growth and yield of durum wheat. Four field experiments, composed of six durum wheat (Triticum turgidum L. var. durum) varieties and three seeding sizes were conducted in north‐east Spain in 2000 and 2001 in randomized complete block designs. The growth of seedlings was dramatically affected by seed size. Large seeds produced greater plot stands, but the plants had fewer tillers, leaves and spikes and less green area and dry weight than plots from small seeds. Grain yield was 16 % greater in plots from large seeds, which resulted in greater biomass, green area index, spikes per m² and heavier kernels than in plots from small seeds. Kernel weight was the yield component most related to grain yield in the three seed sizes. The percentage of yield variation explained by kernel weight increased as the weight of kernels increased. This was a consequence of the use of larger seeds, the same effect being observed when the comparison was made between varieties with different kernel weights. Selection for heavy kernel varieties may help to improve the yield of durum wheat in Mediterranean environments similar to that prevailing in north‐east Spain.  相似文献   

Likening Between the Effect of Drought and Terminal Water-stress Simulated by a Senescing Agent in Triticale     

C. Royo  M. Abaza  C. Canteró  A. Caldero  J. M. Ramos  L. F. García del  Moral 《Journal of Agronomy and Crop Science》1996,176(1):31-38

In 1994 four field experiments were conducted at two sites (northern and southern Spain), and in two environments at each site (irrigated and dryland conditions) in order to compare the effect of drought with the stress caused by chemical treatment with a senescing agent (potassium iodide, KI). A concentration of 0.3 % of KI was applied 10 days after anthesis on triticale Trujillo (X Tnticosecale Wittmack), and four nearisogenic lines derived from it.
The effect of the senescing agent on grain yield was higher in irrigated conditions than in dryland. The reduction in grain yield due to drought was higher than that caused by KI treatment, but both responses were correlated.
Drought and KI treatment had a different effect on yield components since water stress previous to anthesis markedly reduced the number of spikes per m². The effect of the chemical on the number of grains per spike was similar and correlated significantly with the reduction caused by drought. Dry matter accumulation of the grains decreased rapidly following chemical spray due to an important effect of KI on the grain filling rate. No association was found between the reduction in grain weight caused by drought and by KI treatment.  相似文献   

Genetic analysis of days open in beef cattle     

F. Goyache  J.P. Gutiérrez  L.J. Royo 《Livestock Production Science》2005,93(3):283-289

The aim of this work was to analyse the genetic parameters affecting days open (DO) in beef cattle to evaluate its potential as criterion of selection. The present study characterises DO as a trait with considerable genetic variability, relative to that usually found for reproduction traits, especially for heifers and second calving cows. The estimates of heritability for the trait ranged from 0.091 for cows with 10 or more calvings to 0.197 for second calving cows. The genetic correlations estimated for DO in different parities are situated between 0.9 and 1, showing that the genes affecting the trait are substantially the same across parities of the dam. A substantial permanent environment (around 9%) seems to affect DO performance. Permanent environmental factors seem to be especially important in younger cows. Genetic correlation between DO and calving interval was positive and very high (1.0), while those between DO and gestation length and calving date were negative from low to moderate (−0.089 and −0.308, respectively). DO can be used in improvement programs of beef cattle as an early indicator of reproductive performance of the cow.  相似文献   

Disentangling the effects of habitat,connectivity and interspecific competition in the range expansion of exotic and native ungulates     

J. D. Anadón  J. M. Pérez-García  I. Pérez  J. Royo  J. A. Sánchez-Zapata 《Landscape Ecology》2018,33(4):597-608

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Chloride accumulation and partitioning in Barley as affected by differential root and foliar salt absorption under saline sprinkler irrigation     

S. R. Grattan  A. Royo  R. Aragüés 《Irrigation Science》1994,14(3):147-155

Barley is a crop that has been classified as tolerant to soil salinity, but under sprinkler irrigation with saline water it can readily absorb salts through its leaves and develop injury. Experiments using a triple-line-source sprinkler system were conducted on barley between 1989 and 1991 to determine: (1) the specific effects of foliage wetting on the mass of different shoot components; (2) the relative contribution of root and foliar absorption processes to foliar Cl accumulation; and (3) the extent by which these processes affect Cl partitioning in the shoot at the end of the season. Some plants were covered with plastic during the irrigation process to prevent foliar wetting while others remained uncovered. Salinity affected the partitioning of dry matter in the shoots regardless of whether plants were covered during the irrigation process. The organs associated with reproduction, e.g., heads and peduncles, comprised a larger fraction of the total shoot biomass under high salinity than under low salinity, indicating that plants under salinity stress were able to redistribute their dry matter to favor reproductive growth. The Cl concentration of the young leaves sampled from uncovered plants was linearly related (i.e., r ²>0.71) to the Cl concentration of the irrigation water. Equivalent leaves from covered plants also contained a substantial amount of Cl but concentrations were weakly correlated (i.e., r ²<-0.41) with the concentration of Cl in the irrigation water. At low salinity, there were no differences in leaf Cl concentrations between covered and uncovered treatments. In young leaves, differences between these treatments progressively increased with increasing salinity, indicating that the relative contribution of Cl in the leaf from foliar absorbed salts increased with increasing Cl in the irrigation water. Only in the youngest leaves sampled at the end of the season from plants grown at high salinity was the Cl concentration in uncovered plants (foliar plus root-absorbed Cl) found to be more than twice that in covered plants (only root-absorbed Cl) indicating that most of the Cl in young leaves originated from foliar absorption. In addition, only in the youngest leaves (e.g., flag leaves) was the slope of the relationship between leaf-Cl concentration and Cl concentration of the sprinkling water of uncovered plants more than twice that of covered plants, also indicating that foliar-Cl absorption was more substantial than root-Cl absorption. At high salinity, the difference in leaf Cl concentration between covered and uncovered plants was maximum in the youngest leaf (flag leaf), but differences became progressively smaller with increasing leaf age until ultimately concentrations of chloride in leaves older than the flag leaf-2 were highest in covered plants. In older tissue, it was difficult to distinguish which process, foliar or root absorption, was most responsible for leaf-Cl accumulation. These processes may not be entirely independent of one another and much of the Cl in the oldest leaves of uncovered plants could have been derived from foliar sources during the first month of sprinkling, reaching maximal levels, and thereby restricting root-absorbed Cl. Furthermore, since these leaves at the end of the season are more injured and drier than those from covered plants, late-season sprinkler irrigations may have been responsible for leaching some of the Cl out of these necrotic leaves.  相似文献   

Removal of Zn from Aqueous Solutions by Low-Rank Coal     

Solé  Montserrat  Casas  Josep M.  Lao  Conxita 《Water, air, and soil pollution》2003,144(1-4):57-65

The ability of an immature coal (leonardite) to remove zincfrom aqueous solutions was studied as a function of pH,contact time and concentration of metal solutions.Effective removal of Zn²⁺ was demonstrated at pHvalues of 5–6. Kinetic study showed that the adsorption ofZn²⁺ occurs in two phases: a rapid phase followed by aquasi-equilibrium state attained within the first twohours. The adsorption isotherm was measured at 25 °C, using adsorptive solutions at the optimum pH value todetermine the adsorption capacity.  相似文献