Enhancement of alkalinity tolerance in two cucumber genotypes inoculated with an arbuscular mycorrhizal biofertilizer containing Glomus intraradices     

Youssef Rouphael  Mariateresa Cardarelli  Elena Di Mattia  Monica Tullio  Elvira Rea  Giuseppe Colla 《Biology and Fertility of Soils》2010,46(5):499-509

The aim of the present study was to determine whether arbuscular mycorrhizal (AM) inoculation with a biofertilizer containing clays as granular carriers, leek root pieces and Glomus intraradices spores could improve alkalinity tolerance of two cucumber genotypes, and to study the changes induced by AM at agronomical and physiological level. A greenhouse experiment was carried out to determine yield, growth, fruit quality, net photosynthesis (A_CO2), electrolyte leakage, and mineral composition of two cucumber (Cucumis sativus L.) genotypes (hybrid “Ekron” or open-pollinated variety “Marketmore”) with inoculated and noninoculated arbuscular mycorrhizal biofertilizer. Plants were supplied with nutrient solutions at two pH values (6.0 or 8.1). The high pH nutrient solution had the same basic composition with an additional 10 mM NaHCO₃ and 0.5 g l⁻¹ CaCO₃. The percentage root colonization was higher in “Marketmore” (21.8%) than “Ekron” (12.7%). Total and marketable yield and total biomass were significantly higher by 189%, 213%, and 77%, respectively, with Ekron in comparison to those recorded with Marketmore. The highest crop performance with Ekron in comparison to Marketmore was due to the improved nutritional status (higher N, P, K, Ca, Mg, Fe, Mn, and B), higher leaf area, and net photosynthesis. Increasing the concentration of NaHCO₃ from 0 to 10 mM in the nutrient solution significantly decreased yield, plant growth, A_CO2, N, P, Fe, Cu, Zn, Mn, and B concentration in leaf tissue, whereas the electrolyte leakage increased. The inoculated plants under alkaline conditions had higher total, marketable yield, and total biomass than noninoculated plant. Mycorrhizal cucumber plants grown under alkaline conditions had a higher macronutrient concentration in leaf tissue compared to noninoculated plants. The highest yield and biomass production in inoculated plants seems to be related to the capacity of maintaining higher net A_CO2 and to a better nutritional status (high P, K, Mg, Fe, Zn, and Mn and low Na accumulation) in response to bicarbonate stress with respect to −AM plants.  相似文献   

黄河三角洲植被演替过程种群生态位变化研究   总被引：2，自引：2，他引：2  

白世红  马风云  侯栋  王迪 《中国生态农业学报》2010,18(3):581-587

对黄河三角洲天然湿地陆生植被和水生植被两种演替序列的种群生态位宽度和生态位重叠进行了调查研究。结果表明: 陆生植被经过翅碱蓬群落、碱蓬柽柳群落、柽柳群落、白茅群落的演替过程, 每个阶段优势种的生态位宽度都较大。翅碱蓬群落阶段优势种翅碱蓬的生态位宽度达9.99, 碱蓬柽柳群落阶段碱蓬的生态位宽度为9.72, 柽柳群落阶段柽柳的生态位宽度为9.20, 白茅群落阶段白茅的生态位宽度为9.31。水生植被的演替序列为眼子菜金鱼藻群落(沉水植物)阶段、浮萍水鳖群落(浮水植物)阶段、芦苇水烛群落(挺水植物)阶段和杞柳芦苇群落(湿生植物)阶段, 沉水植物阶段优势种眼子菜的生态位宽度为8.62, 浮水植物阶段优势种浮萍生态位宽度为9.23, 挺水植物阶段优势种芦苇的生态位宽度为8.59, 湿生植物阶段优势种杞柳生态位宽度为7.45。生态位宽度计测结果较好地对应着各种群在群落中的地位和作用, 生态位宽度在演替系列中的动态也较好地反映了种群在群落演替过程的数量动态。各演替阶段生态位重叠计算结果表明, 各阶段种群间的生态位重叠值低, 每一阶段内种群间有较高的生态位重叠  相似文献   

Synthesis of angiotensin I-converting enzyme (ACE)-inhibitory peptides and gamma-aminobutyric acid (GABA) during sourdough fermentation by selected lactic acid bacteria     

Rizzello CG  Cassone A  Di Cagno R  Gobbetti M 《Journal of agricultural and food chemistry》2008,56(16):6936-6943

This article aimed at investigating the synthesis of angiotensin I-converting enzyme (ACE)-inhibitory peptides and gamma-aminobutyric acid (GABA) during sourdough fermentation of white wheat, wholemeal wheat, and rye flours. Sourdough lactic acid bacteria, selected previously for proteinase and peptidase activities toward wheat proteins or for the capacity of synthesizing GABA, were used. The highest ACE-inhibitory activity was found by fermenting flour under semiliquid conditions (dough yield 330) and, especially, by using wholemeal wheat flour. Fourteen peptides, not previously reported as ACE-inhibitory, were identified from the water/salt-soluble extract of wholemeal wheat sourdough (IC 50 0.19-0.54 mg/mL). The major part of the identified peptides contained the well-known antihypertensive epitope VAP. The synthesis of GABA increased when the dough yield was decreased to 160. The highest synthesis of GABA (258.71 mg/kg) was found in wholemeal wheat sourdough.  相似文献   

氮肥运筹、配施有机肥和坐水种对春玉米产量与养分吸收转运的影响   总被引：14，自引：4，他引：14  

吴迪  黄绍文  金继运 《植物营养与肥料学报》2009,15(2):317-326

采用田间试验方法,研究了氮肥运筹、配施有机肥和坐水种对春玉米产量与养分吸收转运的影响。结果表明,适宜氮肥运筹方式、配施有机肥和坐水种均能显著增加春玉米产量,氮肥两次追肥处理的产量较一次追肥处理的产量增加10.6%～17.0%,配施有机肥增产10.3%～18.0%,坐水种增产9.1%,配施有机肥并坐水种增产20.4%。氮肥两次追施、配施有机肥及配施有机肥并坐水种均能显著提高春玉米干物质最大积累速率和积累总量,分别提高干物质最大积累速率11.1%～29.5%、28.0%～28.8%和32.1%,依次提高干物质积累总量11.9%～15.5%、21.2%～24.4%和26.9%。氮肥两次追施、配施有机肥和坐水种均可提高N、P、K养分最大吸收速率和吸收总量,养分最大吸收速率以K最高,N居中,P最低;养分吸收总量以N最高,K居中,P最低;养分最大吸收速率出现的时间以K最早（57.2～60.4 d）,N居中（65.3～68.5 d）,P最晚（71.4～74.8 d）,三者均早于干物质积累最大速率出现的时间（82.5～89.3 d）。氮肥两次追施、配施有机肥和坐水种均有利于N、P、K养分由营养体向籽粒的转运量、转运效率、籽粒中养分含量以及氮肥利用率的提高,养分转运效率以P最高（59.3%～66.1%）,其次是N（41.5%～55.1%）,K最低（35.9%～38.0%）。在本实验条件下,施氮量为225 kg/hm2,基肥:拔节肥比1:3,配施有机肥结合坐水种,为最佳施肥处理。  相似文献   

基于RS数据和GIS方法估算区域作物节水潜力   总被引：4，自引：1，他引：4  

彭致功  刘钰  许迪  王蕾 《农业工程学报》2009,25(7):8-12

利用遥感ET数据开展用水定额管理及节水潜力分析等方面的研究,是对传统农业节水研究的有益补充。该文利用分类均值法构建了基于遥感ET数据的作物水分生产函数,考虑耗水较低兼顾水分生产率较高的原则,提出基于水资源脆弱区作物ET定额估算模型,利用该模型计算获得冬小麦及夏玉米的ET定额分别为346.00、313.00 mm;保持现有土地利用结构不变,以作物ET定额为评价标准,通过对超过该作物ET定额的像元进行调整,获得大兴区夏玉米及冬小麦的节水潜力分别为1 176.75、369.27万m3。该研究为利用遥感ET数据开展区域耗水节水潜力的定量化评价进行了有益的探索。  相似文献   

盐碱胁迫对滴灌加工番茄生理生长和干物质积累的影响   总被引：1，自引：0，他引：1  

张继峯  王振华  张金珠  赵娣  窦允清  任作利 《水土保持学报》2019,33(1):270-276

为探讨盐碱胁迫对加工番茄生理生长和和干物质积累的影响,采用桶栽试验方法,人工配制不同盐碱程度的土壤(CK:1.5g/kg,S1:4g/kg,S2:7g/kg,S3:10g/kg),研究滴灌条件盐碱胁迫下加工番茄生长指标、生理指标、干物质积累和产量的变化规律。结果表明:S1处理对加工番茄的生长指标、净光合速率、蒸腾速率、气孔导度、叶片水分利用效率及叶绿素含量均有一定程度的促进作用,但与CK处理无显著性差异(P0.05);S2与S3处理对以上指标均有一定的抑制性,最小值均出现在S3处理,但在生育后期,S2与S3这2个高盐碱处理下,加工番茄的气孔限制值降低,普遍低于S1与CK处理(P0.01),说明在生育后期高盐碱胁迫下加工番茄光合能力的下降主要是由高盐碱胁迫导致叶片气孔关闭的非气孔限制因素造成的;S1处理使加工番茄增产2.1%,而S2与S3处理因抑制加工番茄生长,使其分别减产18.7%和65.4%;果实膨大一期是加工番茄生长发育的一个非常重要时期,此时,加工番茄长势迅速,干物质积累加快,达到全生育期的最大值。研究结果为新疆盐碱地加工番茄栽培管理技术提供理论依据。  相似文献   

Regulation of mycorrhiza development in durum wheat by P fertilization: Effect on plant nitrogen metabolism          下载免费PDF全文

Catello Di Martino  Giuseppe Palumbo  Domenico Vitullo  Patrick Di Santo  Amodio Fuggi 《植物养料与土壤学杂志》2018,181(3):429-440

The aim of this work was to study the effect of arbuscular mycorrhizal fungus Glomus mosseae on growth and nitrogen (N) metabolism of durum wheat (Tritcum durum) under various P soil contents. The analyses were extended to macro and micronutrient tissue concentrations, nitrate reductase and glutamine synthetase activities, as well as protein, aminoacids, pyridine dinucleotides and adenine nucleotides. Arbuscular mycorrhiza increased wheat growth in soil in which P availability was low and nitrate was the dominant N form. The root colonization occurred at the highest level in plants grown in limiting soil P and was inversely related to soil P content. The micorrhizal wheat plants contained also the highest concentrations of macro (P, K, Ca, N) and micronutrients (Fe, Zn, Mn) as well as free amino acids, protein, NAD, NADP, AMP, ADP, ATP in roots and leaves. In particular, the micronutrient tissue concentrations (Zn, Mn) supported that mycorrhiza actively modulated their uptake limiting interferences and optimizing growth better than the plant roots, like a very efficient “rootstock”. Control plants grown at the highest soil P did not reach the same concentration as the mycorrhizal plants. Nitrate reductase activities in the roots of mycorrhizal plants were higher than in the control ones, while glutamine synthetase activities were highest in the leaves. Protein and amino acids concentrations, as well as AMP, ADP, ATP, NAD(P), and NAD(P)H were also higher than in the control. Among the free amino acids in the roots, the high levels of glutamine, asparagine, arginine, support the view that ammonium was transferred through the arbuscules to the root cells where it was re‐assimilated in the cortical cells, forming high N : C ratio‐amino acids. They were transferred to the leaves where all the other N compounds could be largely synthesized using the carbon skeletons supplied by photosynthesis.  相似文献   

高寒草甸土壤微生物功能多样性对氮肥添加的响应   总被引：2，自引：0，他引：2  

代迪  字洪标  杨有芳  阿的鲁骥  陈焱  罗雪萍  王长庭 《水土保持研究》2018,25(2):137-144

采用Biolog-ECO生态板法研究了4个施N水平[0 g/m²（CK）,10 g/m²（N₁₀）,20 g/m²（N₂₀）,30 g/m²（N₃₀）]下土壤理化性质和微生物功能多样性的变化规律。结果表明:中高水平氮肥添加显著增加了速效氮、速效磷含量和根土比,但降低了土壤含水量。施N肥0-10 cm土层平均颜色变化率降低,而10-20 cm土层则被提高,且N₂₀最大。施N肥后降低了0-10 cm土层土壤微生物Shannon-Wiener指数、Pielou指数和McIntosh指数,但10-20 cm土层则被提高。PCA分析表明,施N肥改变了土壤微生物代谢功能类型,糖类、氨基酸、酸类是土壤微生物主要利用的碳源类型。RDA分析表明,在0-10 cm土层,土壤含水量和全氮是影响土壤微生物功能多样性的主要因子,而10-20 cm土层主要受土壤含水量和速效磷的影响。综上,施N肥增加了土壤的有效养分含量,进而改变土壤微生物功能多样性、碳源利用能力和数量。  相似文献   

冬虫夏草中虫草酸的提取条件优化与纯化研究     

李建平  赵秋蓉  吴迪  纪旭东  潘凡达  张峻松 《农产品加工．学刊》2012,(7):49-51

为研究提取条件对冬虫夏草中虫草酸提取率的影响。以虫草酸提取率为考察指标,研究了提取方法、提取时间、提取温度、液固比对虫草酸提取率的影响。结果表明,提取虫草酸的最佳条件为:液固比为10∶1,提取温度为90℃,提取时间为1 h,在此条件下上虫草酸的提取率率为11.36%。利用Sevag法脱蛋白后经重结晶处理后,所得到的虫草酸纯度可达99.92%,这为进一步研究虫草酸的性质提供了物质基础。  相似文献   

大兴安岭生态沟系典型森林植被水源涵养功能的研究     

王黑子来 刘微黄超  徐永波徐迪 《中国农学通报》2011,27(19):33-37

本研究以黑龙江省大兴安岭地区生态沟系3种典型森林类型的持水功能特点为研究对象,应用系统论和水分平衡原理,对森林土壤物理性质、土壤蓄水能力、枯落物蓄水能力以及林冠截留量等进行比较分析。研究表明：林冠截留是林内主要的降水再分配途径,对森林的水分调蓄具有重要作用。水源涵养能力最强的为针阔混交林1259.07 t/hm2,其次为灌木林1046.23 t/hm2,最弱的为落叶松林663.56 t/hm2,针阔混交林林地有着很强的水源涵养和调节能力;灌木林对土壤结构有着良好的改善作用。本研究结果为大兴安岭地区今后开展森林水土保持功能的量化研究及造林的林型结构提供了科学依据。  相似文献