Putative virulence-related genes in Vibrio anguillarum identified by random genome sequencing     

Rodkhum C  Hirono I  Stork M  Di Lorenzo M  Crosa JH  Aoki T 《Journal of fish diseases》2006,29(3):157-166

The genome of Vibrio anguillarum strain H775-3 was partially determined by a random sequencing procedure. A total of 2,300 clones, 2,100 from a plasmid library and 200 from a cosmid library, were sequenced and subjected to homology search by the BLAST algorithm. The total length of the sequenced clones is 1.5 Mbp. The nucleotide sequences were classified into 17 broad functional categories. Forty putative virulence-related genes were identified, 36 of which are novel in V. anguillarum, including a repeat in toxin gene cluster, haemolysin genes, enterobactin gene, protease genes, lipopolysaccharide biosynthesis genes, capsule biosynthesis gene, flagellar genes and pilus genes.  相似文献   

Effects of glycinin on growth performance,immunity and antioxidant capacity in juvenile golden crucian carp,Cyprinus carpio × Carassius auratus     

Min Li  Liang Li  Yi‐Di Kong  Rui Zhu  Zhe Yu  Jing‐Yao Wang  Jing Duan  Li‐Fang Wu 《Aquaculture Research》2020,51(2):465-479

This study was designed to examine the effects of glycinin on growth, digestive ability, immune responses, antioxidant capacity and gene expression levels of golden crucian carp. Golden crucian carp were fed diets containing glycinin at 0, 30, 60, 90 and 120 g/kg, respectively, for 8 weeks. Body weight, weight gain percentage, specific growth rate and feed efficiency ratio were negatively related to the content of glycinin in diet. Activities of protease, acid phosphatase, alkaline phosphatase, lysozyme in hepatopancreas, and activities of catalase, glutathione peroxidase, superoxide dismutase, and total antioxidant capacity in the proximal intestine, mid intestine, distal intestine and hepatopancreas were negatively related to the content of glycinin in diet, whereas malondialdehyde in proximal intestine, mid intestine, distal intestine and hepatopancreas increased directly with the content of glycinin in diet. Furthermore, the relative expressions of TNF‐α and IL‐1β in proximal intestine, mid intestine and distal intestine increased directly with the content of glycinin in diet, whereas the relative expressions of TNF‐α and IL‐1β in hepatopancreas were negatively related to the content of glycinin in diets. The relative expressions of IL‐10 in proximal intestine, mid intestine, distal intestine and hepatopancreas all were negatively related to the content of glycinin in diets. In conclusion, reductions in growth, immunity and antioxidant capacity, intestine inflammation with dysfunction of digestive system occurred in golden crucian carp that fed a diet containing glycinin at 30 g/kg or higher after 8 weeks.  相似文献   

Rainbow trout (Oncorhynchus mykiss,Walbaum ) develop a more robust body shape under organic rearing     

Domitilla Pulcini  Tommaso Russo  Paola Reale  Alexia Massa‐Gallucci  Grace Brennan  Stefano Cataudella 《Aquaculture Research》2014,45(3):397-409

Prolonged exposure to captive conditions has led to the development of a rainbow trout ‘farmed’ phenotype, which is different from that of wild trout. Selection for desirable productive traits in hatcheries has resulted in the development of some morphological traits that are maladaptive in nature. The recent development of organic aquaculture, guided by the well‐being of the fish, could potentially produce a new farmed phenotype that would be more adaptive in nature. In this study, rainbow trout reared in intensive and organic farms were compared by means of shape analysis, to detect patterns of shape variation associated with rearing environment. The results of this study highlight a significant effect of the rearing method on rainbow trout shape: organically reared trout showed a higher body profile, in particular in the head and trunk regions, shorter median fins and a deeper caudal peduncle. A combined effect of density and habitat complexity could have contributed to the observed shape differences: in organic rearing systems, lower densities and steady water could increase territoriality and aggressive interactions, promoting body designs more functional in rapid attacks and escapes.  相似文献   

池蝶蚌组织蛋白酶L1-like基因的克隆及表达特征分析     

彭扣  胡蓓娟  赵大显  王军花  盛军庆  吴娣  史建伍  洪一江 《水产学报》2016,40(10):1556-1566

为了解淡水贝类是否存在组织蛋白酶L的亚型及其亚型的免疫相关作用,本实验利用已构建的池蝶蚌血细胞全长c DNA文库,筛选获得与之同源的EST序列,结合RACE技术进一步克隆了池蝶蚌一个新的组织蛋白酶L基因的c DNA全长,命名为Hs Cts L1-like基因(Gen Bank登录号为KF015273)。该序列全长为1280 bp,5′-非翻译区(5′UTR)为31 bp,3′-非翻译区(3′UTR)为256 bp,开放阅读框区(ORF)为993 bp,编码330个氨基酸,预测蛋白相对分子量为36.86 ku,理论等电点为6.23。序列分析结果显示,Hs Cts L1-like与其他软体动物相对应序列具有共同结构特征,包含信号肽、前肽抑制域和成熟肽三部分,在其他物种中已鉴定的Cts L签名序列标签(ERF/WNIN、GNFD、GCXGG和QCHN等)在Hs Cts L1-like中均可找到。其氨基酸序列同缢蛏Cts L1(AGL33704.1)同源性最高,达67%;与报道的三角帆蚌Cts L(ADV03094)和池蝶蚌中另一个Cts L(AEX88474)仅均为52.91%;系统进化分析表明,Hs Cts L1-like与缢蛏、长牡蛎和合浦珠母贝的Cts L1聚为一分支,推测Hs Cts L1-like属于Cts L家族中的亚型1。实时荧光定量PCR(q RT-PCR)检测显示,Hs Cts L1-like m RNA在肝脏中表达量最高,其次是卵巢和精巢。注射鳗弧菌后,血细胞和肝脏Hs Cts L1-like m RNA转录水平显著升高,暗示其是一个免疫有关的基因,参与了池蝶蚌的先天免疫应答反应。  相似文献   

海洋底部鱼类加工行业价值链提升与优化——基于山东省鱼类企业的调研     

高金田  张迪  李京梅 《渔业经济研究》2013,(5):90-95

中国是水产品加工大国,近年来水产品加工业的发展受到国际环境及国内因素制约,比较优势逐渐下降。本文基于底部鱼类加工企业的实地调查数据,采用价值链分析方法,估算我国水产加工环节中的价值增量,分析加工业中存在的问题及其制约因素,进一步提出相应的改进措施,以提升我国底部鱼类加工业的竞争优势。  相似文献   

红螯光壳螯虾组织蛋白酶L基因的克隆及维生素C对其表达的影响     

吴东蕾  左迪  黄有辉  马长安  赵云龙 《水产学报》2014,38(11):1818-1827

为深入了解红螯光壳螯虾组织蛋白酶L基因的表达特性及维生素C对其表达的影响,实验利用RACE-PCR技术及荧光定量PCR技术,从红螯光壳螯虾肝胰腺中克隆得到组织蛋白酶L基因cDNA全长序列,命名为CqCatL(GenBank登录号:KJ913663),同时检测了该基因在红螯光壳螯虾各个组织及添加了不同浓度维生素C的组别中的表达。结果显示,该基因全长1 810 bp,开放阅读框长度为1 026 bp,编码341个氨基酸残基,预测的分子量和等电点(pI)分别为37.63 ku和5.17。同源性分析结果显示,该基因编码的蛋白与其他虾蟹类有较高的相似性,说明组织蛋白酶L基因在甲壳动物具有较高的保守性。组织荧光定量PCR结果显示,CqCatL基因在红螯光壳螯虾的多个组织中均有表达,其中肝胰腺中表达量最高,其次为血细胞,在肠及触角腺中也有一定量的表达。在基础饲料中添加不同水平的维生素C后,CqCatL基因的表达量也存在明显差异,其中维生素C添加量为400 mg/kg的组别中该基因的表达量最高。研究表明,组织蛋白酶L基因在红螯光壳螯虾的生长发育过程中有重要的作用,且其表达量受维生素C的影响。  相似文献   

长江中上游圆口铜鱼的种群死亡特征及其物种保护   总被引：1，自引：0，他引：1  

杨志  乔晔  张轶超  朱廸  常剑波 《水生态学杂志》2009,30(2):50-55

根据葛洲坝(1998～2007年)、重庆(2006～2007年)和合江江段(1998～2005年)的渔业资源调查资料,对圆口铜鱼的种群死亡特征进行了评估,并利用Beverton-Holt模型的单位补充量渔获量方程,分析和探讨了长江中上游圆口铜鱼资源的合理利用.结果表明:1)3个江段各年间的圆口铜鱼开发率和捕捞死亡系数均远远大于相应年份最大允许的开发率和捕捞标准的基准尺度F0.1,3个江段的圆口铜鱼资源均处于严重过度捕捞状况;2)葛洲坝江段圆口铜鱼的最适开捕年龄为4龄(体长330mm),重庆和合江江段圆口铜鱼的开捕年龄应至少为5龄(体长375 mm).为保护圆口铜鱼的资源,建议葛洲坝江段三层流刺网的网日(2a)应大于75 mm为宜,重庆和合江江段的网目(2a)应不小于90 mm且同时需要控制各种渔具的日均作业次数.  相似文献   

Processing tomato quality as affected by irrigation scheduling   总被引：5，自引：0，他引：5  

Fabio Favati  Stella Lovelli  Fernanda Galgano  Vito Miccolis  Teodoro Di Tommaso  Vincenzo Candido 《Scientia Horticulturae》2009,122(4):562-571

In Southern Italy the paucity of water is a very important problem and due to the fundamental importance of the water for the processing tomato, in this study the influence of irrigation on processing tomato quality was investigated in the years 2002 and 2003. A hybrid processing tomato (Lycopersicon esculentum L. Mill cv. Ability) was treated with different water regimes obtained combining amount of water and irrigation interval. The effects of the trials were evaluated taking into account the physical and chemical characteristics of the fruits, as well as the content in antioxidants moieties. Furthermore, the relationship among all the quality parameters and the seasonal amount of irrigation water was evaluated.The results, comparing different water regimes obtained combining amount of water and irrigation interval, gave useful indications on the possibility to improve nutritional tomato quality by reducing irrigation water during tomato cropping. In particular, relatively to the Mediterranean areas of tomato cultivation and to the texture of the soil considered in this study, the best compromise between quality and quantity of the processing tomato fruit was achieved both with the extension of the irrigation interval (L40 or L60) and with the limitation of irrigation volume for the second part of the tomato crop cycle (thesis 100-50). Extending the irrigation interval and limiting irrigation volume for the second part of the tomato crop cycle, appeared to be the best management strategy to optimize the yield and nutritional quality of processing tomato.  相似文献   

甘蓝与萝卜属间杂交及其胚挽救技术优化     

扈新民  李锡香  梁燕  方智远  沈镝  邱杨 《中国蔬菜》2009,1(10):7-12

以甘蓝显性雄性不育系C08-11为母本,以萝卜自交系Q07-12为父本进行属间杂交,对杂交子房和胚珠进行离体培养,筛选适合甘蓝与萝卜属间杂种胚挽救的适宜取材时间、培养基及外源添加物。结果表明,胚珠培养优于子房培养;所有处理子房培养仅获得3株杂交苗,取材时间以授粉后第9天为宜,B5培养基中添加合适的生长调节剂、水解酪蛋白和活性炭为子房培养所必需;胚珠培养各处理共获得29株杂交苗,适宜取材时间为蕾期授粉后第14天、花期授粉后第16天,对生长调节剂的需求取决于不同取材时期及培养基。  相似文献   

敌百虫对鲫鱼的急性毒性研究   总被引：3，自引：0，他引：3  

卢彤岩  吴京蔚  王获  王进 《水产学杂志》2008,21(1):68-73

通过96h急性毒性实验敌百虫对鲫鱼的LC50为63.97mg/L,95%可信限为1.81±0.048 mg/L,95%可信区间为57.81～72.11 mg/L.分别选取1/2、1/4、1/8及1/16LC50为染毒浓度,于实验开始后24、48、72及96h采样分析,结果表明随着暴露时间的延长,各浓度组实验鱼血浆胆碱酯酶的活性均有一定的升高,但各浓度组间没有表现出明显的区别.实验剂量下的敌百虫对鲫鱼血浆CST活性有一定的影响,呈现出较为明显的先扬后抑的结果.1/2LC50实验组血浆CAT在实验进行至96h活性明显高于对照组,而其他剂量组在实验期间CAT活性没有明显的改变.本实验中敌百虫对鲫鱼的遗传性在微核率上未表现出差异,但高浓度核异常率在实验24、48h明显高于对照组.  相似文献