沙尘暴应急监测方法研究     

唐书彦  杜宇红  金向红  张同文  李朝晖 《干旱区资源与环境》2004,(Z1)

在沙尘暴应急监测方法研究中,通过对总悬浮颗粒物采样仪器及各种采样参数的研究确定和降尘采样器的研制,制定出沙尘暴应急监测方法。  相似文献   

新建县沙生植被的保护与开发利用     

杨欢  曾慧卿 《江西林业科技》2004,(2):48-49

以农业生产为支柱产业的新建县地处鄱阳湖流域，区域内沙生植被较脆弱。随着经济与人口的快速增长及农、林、旅游业的大发展，如何保护县域内沙生植被、更好的开发和利用这种资源十分重要。在分析新建县沙生植被现状及影响其生长的主要因素的基础上，就该地区内沙生植被的保护和开发利用提出建议。  相似文献   

云南松人工林木材百度干燥特性实验研究     

曾黄元  杜官本  孙照斌 《林业机械与木工设备》2005,33(10):21-23

采用百度试验法对云南松人工林木材进行了干燥特性的研究.结果表明：水煮后的木材干燥质量优于非处理木材,截面变形程度轻,且干燥时间缩短,并提出了云南松的干燥基准.  相似文献   

枣胚培技术体系的建立   总被引：8，自引：1，他引：8  

杜学梅  李登科  王永康  隋串玲  樊保国 《园艺学报》2005,32(3):496-499

 以‘六月鲜’枣为试材, 对花后12～82 d的幼胚进行培养。结果表明, 适宜于胚挽救的最小胚龄为花后50 d左右; 在胚形成前进行离体培养, 蔗糖浓度以7%为宜, 而胚形成后离体培养以5%蔗糖为宜; 暗培养可促进离体胚的生长发育, 且有利于生根; 植物生长调节剂及有机添加物极显著地影响着胚培效果。花后70～72 d胚龄的胚适宜培养基为1 /2MS + IBA 0.4 (mg/L, 单位下同) +BA 0.8 +LH 500 + GA₃5 + 5%蔗糖。胚培苗经扩繁后转入1 /2 MS + IBA 1.0 + IAA 0.06 + 2%蔗糖培养基上, 获得了健壮生根苗,生根苗移栽成活率达85%以上。  相似文献   

早熟西瓜新品种大宝的选育   总被引：1，自引：0，他引：1  

王铁臣  王满保  黄环 《中国蔬菜》2005,1(8):25-26

大宝西瓜是以347为母本,355为父本配制的一代杂种,早熟,生长势中等,主蔓第7～8节着生第1雌花。全生育期93d(天)左右,果实发育期28～30d(天),果实椭圆形,果形指数1.3左右,单瓜质量3～4kg。果皮墨绿色,厚0.8～1.0cm,有白霜,坚韧,耐运输。果肉红色,口感好,中心含糖量9.5%～10.0%,最高可达11.0%。中抗枯萎病,适于华北地区早春保护地或露地早熟栽培,每667m2产量2500kg左右。  相似文献   

工厂化猪场不同猪群死亡率与季节的关系初探     

杜俊成 《家畜生态》2005,(6)

通过对哺乳仔猪群、保育群、育成群各月死亡率的统计分析发现,冬季低温对哺乳仔猪群、保育群死亡率的影响远远大于夏季高温的影响。冬季低温对保育群的死亡率影响最大,而对育成群几乎没有影响。夏季高温对不同猪群的死亡率有不同程度的影响力。不同猪群对温度反应的快慢、强度及持续期各不相同。  相似文献   

乌梁素海生态恢复机理与工程技术的研究   总被引：2，自引：1，他引：2  

尚士友  杜健民  厚福祥  申庆泰  王丽敏 《干旱区资源与环境》2003,17(6):50-54

在我国干旱区典型草型湖泊—乌梁素海设立试验基地 ,进行生态恢复工程试验研究。研究表明 :乌梁素海受富营养化影响 ,正在向芦苇沼泽—碱蓬盐化草甸—白刺荒漠方向演化。生态恢复机理是 :( 1 )减少大型水生植物有机氮、磷向底质的沉降 ,削弱内源性营养物负荷的积累与储备 ;( 2 )减少大型水生植物死亡后矿化成氨态氮 ;( 3)控制底质中有机氮向水体释放生成氨态氮。采用机械化方式收割大型水生植物转移营养盐 ,抑制生物促淤效应以及对芦苇区进行园田化生态管理是乌梁素海生态恢复的关键技术 ,也是乌梁素海环境与资源持续发展的核心问题  相似文献   

Association of polymorphism of Fc receptor γ chain gene at position-29 in promoter with the susceptibility to systemic lupus erythematosus in southern Chinese     

ZHOU Shu-lu  YE Ren-gao  LIU Xiao-bo  ZHANG Hong  XU Han-shi  DU Yong  LI You-ji  YANG Nian-sheng  YANG Xiao  YU Xue-qing 《园艺学报》2003,19(6):782-785

AIM:To detect the association between the polymorphism of Fc receptor γ chain gene at position-29 in promoter and systemic lupus erythematosus(SLE).METHODS:The genotypes at position -29 in promoter of Fc receptor γ chain gene were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in 180 patients with SLE and 140 ethnically matched controls in southern China.RESULTS:The frequencies of TT genotype(33.3%) and T allele (54.4%) at position -29 in patients with SLE were significantly higher than those in controls (17.2% and 42.9%, respectively), whereas, the frequencies of GG genotype (24.4%) and G allele (45.6%) in patients with SLE were remarkably lower than those in controls (31.4% and 57.1%, respectively) (P＜0.05). The TT genotype and T allele at position -29 were not associated with lupus nephritis in SLE patients (P＞0.05).CONCLUSION:Our results indicate that the T allele at position -29 in promoter of Fc receptor gene probably contributes to the susceptibility to SLE, but does not play a role in the occurrence of lupus nephritis.  相似文献   

Antisense drug targeting with VEGF mRNA designed by computer aid and inhibitory effects on K562 cell line in vitro     

FEI Jia  ZHANG Huan 《园艺学报》2003,19(5):645-648

AIM:The effective antisense sequences targeted VEGF mRNA with computer software would be screened and designed, and effect of them on growth K562 cells and protein expression of VEGF were studied with experiments.METHODS:Seven antisense sequences were selected and synthesized, which consisted of 18-20 deoxynucleotide acid and were modified with phosphorothioate, according to principle of low free energy of overall △G₃₇ Overall. Cell growth was assayed by trypan blue dye exclusion assay and level of VEGF protien in the media was determined by ELISA.RESULTS:Six of seven sequences were capable of inhibing growth of K562 cells and downregulating the VEGF protein expression significantly, compared with Scrambed control group. It was found that there was a close correlation between low level of overall △G₃₇ and antisense effectiveness (r=0.887,P<0.01).CONCLUSION:VEGF mRNA antisense oliogdeoxynucleotides, which were designed by computer software of RNAstructure, were able to inhibit growth of K562 and its protein expression. The VEGF mRNA may be new target attached by drugs. At same time, the computer aided design is useful methods to obtain the effective antisense.  相似文献   

Bcl-2 antisense oligodeoxynucleotide increases the sensitivity of HL60 and K562 cells to daunorubicin     

LEI Xiao-yong  ZHANG Huan  HE Dong-mei 《园艺学报》2003,19(2):194-197

AIM:To investigate whether the bcl-2 antisense oligonucleotide increases the sensitivity of HL60 and K562 cell lines to daunorubicin.METHODS:IC50 for HL60 and K562 was determined with MTT method, the expression levels of Bcl-2 protein were assayed by immunofluorescence using fluoresce isothiocyanate labeling. In addition, apoptosis was detected by morphological observation and flow cytometric analysis of DNA fragmentation.RESULTS:It was found that the two oligonucleotides directed against the coding region and the translation initiation of bcl-2 mRNA, combined respectively with daunorubicin, inhibited expression of bcl-2 protein, increased apoptosis in HL60 and K562 cells, and decreased IC50 of daunorubicin significantly (P＜0.05). Compared to the antisense oligonucleotide directed against the translation initiation of bcl-2 mRNA, the antisense oligonucleotide directed against the coding region showed stronger effects in the aspects of increasing the sensitivity of HL60 cells to daunorubicin (P＜0.05).CONCLUSIONS:These two antisense sequences in the translation initiation and the coding region of bcl-2 mRNA increased the sensitivity of HL60 and K562 cell lines to daunorubicin in a sequence-specific manner.  相似文献