Inhibition of growth hormone secretagogue receptor antagonist, [D-Lys-3]-GHRP-6, in adipogenesis of ovine and rat adipocytes     

Sang-gun ROH  Yeon-hee HONG  Daisuke HISHIKAWA  Hiroaki TSUZUKI  Hisae MIYAHARA  Yukihiko NISHIMURA  Chizu GOTO  Yasuki SUZUKI  Ki-choon CHOI  Hong-gu LEE  Shinichi SASAKI 《Animal Science Journal》2005,76(4):381-386

Ghrelin and growth hormone secretagogues receptor (GHS‐R or ghrelin receptor) have been reported as being one of the factors of adipogenesis in adipocytes. To investigate the involvement of ghrelin and GHS‐R in adipocytes, the effect of the GHS‐R antagonist, [D‐Lys‐3]‐GHRP‐6 (His‐D‐Trp‐D‐Lys‐Trp‐D‐Phe‐Lys‐NH₂), on the process of adipogenesis in ovine and rat adipocytes was evaluated. [D‐Lys‐3]‐GHRP‐6 (10^?7 mol/L) significantly inhibited adipogenic differentiation of ovine and rat preadipocytes prepared from adipose tissues. The level of peroxisome proliferator activated receptor (PPAR)‐γ2 mRNA, an adipogenic marker, was decreased during the differentiation of adipocytes treated with [D‐Lys‐3]‐GHRP‐6 for 10 days. Ghrelin stimulated adipogenesis, also causing an increment of glycerol‐3‐phosphate dehydrogenase and upregulation of PPAR‐γ2. Furthermore, the antilipolytic effect of ghrelin was attenuated by treatment with [D‐Lys‐3]‐GHRP‐6 in both types of isolated adipocytes. Overall, the results of the present study highlight that GHS‐R in adipogenesis can be blocked by treatment with [D‐Lys‐3]‐GHRP‐6.  相似文献   

Malignant melanoma of the eyelid in a red squirrel (Sciurus vulgaris orientis)     

Fukui D  Bando G  Kosuge M  Yamaguchi M  Shibahara T  Kadota K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(3):261-264

A 6-year-old male red squirrel (Sciurus vulgaris orientis) developed bilateral tumors of upper and lower eyelids. The tumors in the left lid recurred despite surgical removal. Necropsy revealed metastasis to the lung. The neoplastic cells were epithelioid and highly pleomorphic, and only a few cells contained melanin granules. Occasionally melanoma cells were immunoreactive for S100, neuron-specific enolase and vimentin, and a small number of cells for cytokeratin. Ultrastructurally, the presence of premelanosomes was confirmed in the cytoplasm. Possible presence of cytokeratin-positive neoplastic melanocytes should be taken into account when differentiating a nonpigmented epithelioid melanoma from other tumors such as anaplastic carcinomas.  相似文献   

A meningioma with hyperintensity on T1-weighted images in a dog     

Hasegawa D  Kobayashi M  Fujita M  Uchida K  Orima H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(6):615-617

A male six-year-old Pomeranian showed recurrent seizures and progressive left hemiparesis. MRI revealed a mass in the right paramedian frontal-temporal region with hyperintensity on T1-weighted (T1W) and mixed-intensity on T2-weighted (T2W) images. After gadolinium enhancement, the mass was enhanced homogenously and demonstrated the dural tail sign. Surgical resection of mass was performed and its histological diagnosis was meningioma. The meningioma of this case had a high cellular density with some lipid contents and intra- and extra-tumor hemorrhage, however, calcification was not found. These cellular characteristics may contribute to a higher signal intensity on T1W imaging.  相似文献   

The relationship between the viral RNA level and upregulation of innate immunity in spleen of cattle persistently infected with bovine viral diarrhea virus     

Yamane D  Kato K  Tohya Y  Akashi H 《Veterinary microbiology》2008,129(1-2):69-79

  相似文献   

Successful use of prednisolone and radiation therapy in a dog with intracranial histiocytic sarcoma     

Tomoko TAKAHASHI  Yumiko KAGAWA  Daisuke ITO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(11):1782

The ideal treatment for intracranial histiocytic sarcoma (HS) remains unclear. Herein, we report a case of intracranial HS that was successfully treated using prednisolone and radiation therapy. The patient was a 9-year-old spayed female Pembroke Welsh Corgi that presented with epileptic seizures. Magnetic resonance imaging revealed a contrast-enhancing mass adjacent to the right piriform lobe. Prednisolone administration (1 mg/kg/day) decreased the lesion size. Additional palliative radiation therapy (total dose, 37 Gy) resulted in complete disappearance of the lesion. However, on day 164, the dog’s neurological signs deteriorated, and she was euthanized. Necropsy revealed an intracranial metastasis of HS via the cerebrospinal fluid without any extracranial metastasis. Nonetheless, combined prednisolone and radiation therapy might be effective in treating intracranial HS.  相似文献   

Synthesis and antifungal activity of the proposed structure of a volatile compound isolated from the edible mushroom Hypsizygus marmoreus     

Nguyen Thi Ngoc Anh  Daisuke Miyaji  Kumiko Osaki-Oka  Tatsuo Saito  Atsushi Ishihara  Arata Yajima 《Journal of Pesticide Science》2022,47(1):17

We synthesized the proposed structure of an antifungal compound detected in the culture broth of the edible mushroom Hypsizygus marmoreus. Using the Evans aldol and Abiko–Masamune aldol reactions as the key steps, we synthesized all of the stereoisomers of the compound with high stereoselectivity. The GC retention times and the fragmentation patterns in the mass spectra of the synthesized isomers did not match those of the natural product. Therefore, this result may imply that it is necessary to reisolate the natural product and reconsider its structure. All of the synthesized isomers were found to exhibit antifungal activity against the phytopathogenic fungus Alternaria brassicicola. Due to their simple structures, the obtained isomers could be lead compounds for new pesticides.  相似文献   

Reproduction affects partitioning between new organs of a pulse of 15N applied during seed ripening in Fagus crenata     

Han  Qingmin  Kabeya  Daisuke  Inagaki  Yoshiyuki 《New Forests》2020,51(5):739-752

Seasonal internal nitrogen (N) cycling is an important strategy for trees to uncouple growth from N acquisition. While N uptake, allocation and storage has been intensively studied in association with leaf phenology and vegetative growth, influence of reproduction on these key processes is still poorly understood. Therefore, we applied pulse ¹⁵N labelling to three fruiting and three non-fruiting trees in a 92-year-old Fagus crenata forest on 18 July and traced ¹⁵N content per organ dry mass (¹⁵N_excess) in all new shoot organs from the upper crowns periodically until leaf fall. The amount of ¹⁵N_excess recovered in the whole new shoots in fruiting trees did not differ from non-fruiting individuals, although up to 70% of ¹⁵N_excess was recovered in fruits of fruiting trees but 87% in leaves of non-fruiting individuals. In addition, dramatic increase in ¹⁵N_excess amount in nuts was accompanied by about twofold increase in nut N content. These results indicate that new N uptake from the soil contributed greatly to seed ripening, which in turn resulted in less allocation to leaves in fruiting trees. In non-fruiting individuals, on the other hand, ¹⁵N_excess allocated to leaves was not accompanied by concomitant increase in leaf N content because biomass growth had ceased when ¹⁵N was applied. These results suggest that N uptake in the late growing season contributed to internal N storage in non-fruiting trees. These reproduction-related variations in seasonal N cycle have implications for N dynamics in the plant–soil system during environmental change.

  相似文献   

Mapping of expressed sequence tag markers with a cDNA-amplified fragment length polymorphism method in Japanese quail (Coturnix japonica)     

Shinji SASAZAKI  Tomoko HINENOYA  Daisuke FUJIMA  Shinichi KIKUCHI  Akira FUJIWARA  Hideyuki MANNEN 《Animal Science Journal》2006,77(1):42-46

In our previous study, a Kobe‐NIBS Japanese quail (KNQ) linkage map was constructed mainly using amplified fragment length polymorphism (AFLP) markers. In order to compare chicken and quail chromosomes, we developed expressed sequence tag (EST) markers derived from cDNA‐AFLP fragments and localized these markers on the linkage map. Using a total of 128 AFLP primer combinations, 24 polymorphic bands were obtained between a neurofilament‐deficient mutant quail line male and a muscular disorder quail line female, which were the parents of the KNQ resource family. Nine of the 24 markers were mapped by linkage analysis. These markers were mapped to seven linkage groups, namely 1, 2, 3, 6, 8, 15 and 42. A subsequent homology search using chicken genome sequences strongly suggests that these linkage groups correspond with chicken chromosomes 1, 2, 3, 5, 15, 23 and 26.  相似文献   

Effect of environmental enrichment by providing trees as rubbing objects in grassland: Grazing cattle need tree-grooming     

Daisuke KOHARI  Takami KOSAKO  Michiru FUKASAWA  Hideharu TSUKADA 《Animal Science Journal》2007,78(4):413-416

We examined the role of trees as grooming objects in grazing pasture and investigated their necessity. Four grazing Japanese Black cows were used. Experimental pastures with restricted (RST) and released (RLS) grooming with trees were established. Examination was carried out for 24 h in each RST and RLS. The respective frequencies and duration of self‐grooming, allo‐grooming (social grooming) and grooming with trees was recorded by a continuous scan sampling method, and maintenance behavior was recorded by a 2‐min group animal scan sampling method. Grooming with trees in RLS occurred 29 ± 12 times/24 h and for 1342 ± 475 s/24 h. Total frequencies and duration of grooming in RLS tended to increase from that in RST (P = 0.08, P = 0.06, respectively). However, self‐grooming and allo‐grooming exhibited no differences. The results indicate that cattle may enrich their grooming through the use of trees if the latter are available. Furthermore, they may not substitute grooming with trees with other forms of grooming if no trees are available in the grassland. No difference was observed in the general proportion of their maintenance behavior. These results suggest that trees in grassland provide a good environmental enrichment object that satisfies cattle's potential needs of grooming and did not restrict their general behavior.  相似文献   

GM2-gangliosidosis variant 0 (Sandhoff-like disease) in a family of Japanese domestic cats     

Yamato O  Matsunaga S  Takata K  Uetsuka K  Satoh H  Shoda T  Baba Y  Yasoshima A  Kato K  Takahashi K  Yamasaki M  Nakayama H  Doi K  Maede Y  Ogawa H 《The Veterinary record》2004,155(23):739-744

A five-month-old, female Japanese domestic shorthair cat with proportionate dwarfism developed neurological disorders, including ataxia, decreased postural responses and generalised body and head tremors, at between two and five months of age. Leucocytosis due to lymphocytosis with abnormal cytoplasmic vacuolations was observed. The concentration of G(M2)-ganglioside in its cerebrospinal fluid was markedly higher than in normal cats, and the activities of beta-hexosaminidases A and B in its leucocytes were markedly reduced. On the basis of these biochemical data, the cat was diagnosed antemortem with G(M2)-gangliosidosis variant 0 (Sandhoff-like disease). The neurological signs became more severe and the cat died at 10 months of age. Histopathologically, neurons throughout the central nervous system were distended, and an ultrastructural study revealed membranous cytoplasmic bodies in these distended neurons. The compound which accumulated in the brain was identified as G(M2)-ganglioside, confirming G(M2)-gangliosidosis. A family study revealed that there were probable heterozygous carriers in which the activities of leucocyte beta-hexosaminidases A and B were less than half the normal value. The Sandhoff-like disease observed in this family of Japanese domestic cats is the first occurrence reported in Japan.  相似文献