Tuberculosis survey of free-ranging marsh deer (Blastocerus dichotomus) in Brazil.     

Janaina O Luna  Manoel A A Santos  Edison L Durigon  Jo?o P Araújo Júnior  José M B Duarte 《Journal of zoo and wildlife medicine》2003,34(4):414-415

Esophageal-pharyngeal fluids from 53 free-ranging marsh deer (Blastocerus dichotomus) captured for a research program in the state of Mato Grosso do Sul, Brazil, were assayed for tuberculosis. Total DNA was extracted. amplified by polymerase chain reaction using specific primers for Mycobacterium tuberculosis complex (M. tuberculosis, M. bovis, M. microti, and M. africanum), and observed by agarose gel electrophoresis stained with ethidium bromide. All samples were negative. This, along with necropsy and histopathology data, suggests that these animals are not shedding and probably do not have active disease.  相似文献   

Nitrogen mineralization in sandy loam soils under an intensive double-cropping forage system with dairy-cattle slurry applications   总被引：4，自引：0，他引：4  

H. Trindade  J. Coutinho  S. Jarvis  N. Moreira 《European Journal of Agronomy》2001,15(4):281-293

Nitrogen (N) mineralization and soil mineral N contents were measured at 2-week intervals over a 2-year period (June 1994–May 1996) on two different sites in the North West region of Portugal. The experiment was established in fields, which had for many years been under a double-cropping forage system with maize from May to September and a winter crop (mixture of cereals and Italian ryegrass) during the rest of the year. In addition to N fertilizers, dairy-cattle slurry was applied regularly at the sowing of each crop. On this intensive forage system, quantification of N released from slurry, crop residues and soil organic matter becomes important when better N use efficiency and reduced environmental impact from agricultural practices are required. Net N mineralization rates of the 0–10 cm soil layer fluctuated considerably between consecutive incubation periods and ranged from −0.88 to 1.87 mg N kg⁻¹ day⁻¹ with annual average rates of between 0.41 and 0.65 mg N kg⁻¹ day⁻¹. The total N mineralized in the 10 cm depth soil layer reached values between 122 and 224 kg N ha⁻¹ year⁻¹, showing that mineralization was a very important N source for the crops. The amounts of N released during the cold season (November–February) were equivalent to 27–48% of the yearly total. Regression analysis indicated that seasonal variation in N mineralization was only poorly explained by soil moisture and temperature. The changing balance during the year between soil moisture and temperature will contribute to the relatively constant N mineralization rates. Soil mineral N contents during the maize crop were high and exceeded the nutrient requirements for the optimum yield of this crop. Under the climatic conditions of the region and due to the poor development of the winter crop plants at the time, the mineral N left in the soil after the maize crop and released by mineralization during the cold season is particularly vulnerable to nitrate leaching losses.  相似文献   

QTL mapping of volatile compounds in ripe apples detected by proton transfer reaction-mass spectrometry     

Elena?Zini  Franco?Biasioli  Flavia?Gasperi  Daniela?Mott  Eugenio?Aprea  Tilmann?D.?M?rk  Andrea?Patocchi  Cesare?Gessler  Matteo?KomjancEmail author 《Euphytica》2005,145(3):269-279

The availability of genetic linkage maps enables the detection and analysis of QTLs contributing to quality traits of the genotype. Proton Transfer Reaction Mass Spectrometry (PTR-MS), a relatively novel spectrometric technique, has been applied to measure the headspace composition of the Volatile Organic Compounds (VOCs) emitted by apple fruit genotypes of the progeny ‘Fiesta’ × ‘Discovery’. Fruit samples were characterised by their PTR-MS spectra normalised to total area. QTL analysis for all PTR-MS peaks was carried out and 10 genomic regions associated with the peaks at m/z = 28, 43, 57, 61, 103, 115 and 145 were identified (LOD > 2.5). We show that it is possible to find quantitative trait loci (QTLs) related to PTR-MS characterisation of the headspace composition of single whole apple fruits indicating the presence of a link between molecular characterisation and PTR-MS data. We provide tentative information on the metabolites related to the detected QTLs based on available chemical information. A relation between apple skin colour and peaks related to carbonyl compounds was established. The two authors contributed equally to this work.  相似文献   

Arabinogalactan proteins may facilitate the movement of pollen tubes from the stigma to the ovules in Actinidia deliciosa and Amaranthus hypochondriacus     

Sílvia Coimbra  Cristina Duarte 《Euphytica》2003,133(2):171-178

Sexual plant reproduction is a complex process that involves a series of interactions between the male gametophyte and the different cell types of the pistil. These interactions are believed to direct the pollen tube growth until its final target, the embryo sac. Arabinogalactan proteins are complex proteoglycans that are believed to be involved in these processes. The pistil is enriched in these highly glycosylated proteins and we provide results that show the selective presence of different AGP epitopes at the surface of the cells or in the ECM of the tissues that correspond exactly to the pollen tube growth pathway in Amaranthus hypochondriacus and Actinidia deliciosa. We also show that in Actinidia deliciosa, which is a dioecious plant with the male flowers having rudimentary ovaries where fertilization does not occur, there is no presence at all of the epitopes recognised by the monoclonal antibodies utilized in this study. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Inheritance of angular leaf spot resistance in common bean line BAT 332 and identification of RAPD markers linked to the resistance gene     

Eveline Teixeira Caixeta  Aluízio Borém  Samir de Azevedo Fagundes  Silvia Niestche  Everaldo Gonçalves de Barros  Maurílio Alves Moreira 《Euphytica》2003,134(3):297-303

The existence of genetic variability for angular leaf spot (ALS) resistance in the common bean germplasm allows the development of breeding lines resistant to this disease. The BAT 332 line is an important resistance source to common bean ALS. In this work we determined the inheritance pattern and identified RAPD markers linked to a resistance gene present in BAT 332. Populations F₁, F₂,BCs and BCr derived from crosses between BAT 332 and cultivar Rudá were used. Rudá is a commercial cultivar with carioca type grains and susceptible to ALS. The resistance of BAT 332 to race 61.41 of the pathogen was confirmed. Segregation analysis of the plants indicated that a single dominant gene confers resistance. For identification of RAPD markers linked to the resistance gene, bulk segregant analysis (BSA) was used. Two RAPD markers,OPAA07₉₅₀ and OPAO12₉₅₀, linked in coupling phase at 5.10 and 5.83 cM of this gene, respectively, were identified. These molecular markers are important for common bean breeders and geneticists as source of genetic information and for marker assisted selection in breeding programs. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Interaction of the mutant genes B,og c,hp and t in the coloring of tomato fruit     

Thiago Matos Andrade  Wilson Roberto Maluf  Celso Mattes de Oliveira  Luiz Antônio Augusto Gomes  Daniela Costa Santos  Regis de Castro Carvalho  Ranoel José de Sousa Gonçalves  Álvaro Carlos Gonçalves Neto 《Euphytica》2015,205(3):773-783

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Use of molecular markers to accelerate the breeding of common bean lines resistant to rust and anthracnose     

Fábio G. Faleiro  V.A. Ragagnin  M.A. Moreira  E.G. de Barros 《Euphytica》2004,138(3):213-218

The main goal of this work was to introduce resistance genes for rust, caused by Uromyces appendiculatus, and anthracnose, caused by Colletotrichum lindemuthianum, in an adapted common bean cultivar through marker-assisted backcrossing. DNA fingerprinting was used to select plants genetically closer to the recurrent parent which were also resistant to rust and to race 89 of C. lindemuthianum. DNA samples extracted from the resistant parent (cv. Ouro Negro), the recurrent parent (cv. Rudá), and from BC₁, BC₂ and BC₃ resistant plants were amplified by the RAPD technique. The relative genetic distances in relation to the recurrent parent varied between 9 and 59% for BC₁, 7 and 33% for BC₂, and 0 and 7% for BC₃ resistant plants. After only three backcrosses, five lines resistant to rust and anthracnose with, approximately, 0% genetic distance in relation to the recurrent parent were obtained. These lines underwent field yield tests in two consecutive growing seasons and three of them presented a good yield performance, surpassing in that sense their parents and most of the reference cultivars tested.  相似文献   

Simulation of the Dispersion of Pollutants Using two Approaches for the Case of a Low Source in the Sbl: Evaluation of Turbulence Parameterisations     

D. M. Moreira  J. C. Carvalho  A. G. Goulart  T. Tirabassi 《Water, air, and soil pollution》2005,161(1-4):285-297

An Eulerian model and a Lagrangian particle model are used to study the dispersion of a contaminant released from a low source in the Stable Boundary Layer (SBL) using two different turbulence parameterisations. The Eulerian model is based on the solution of the advection–diffusion equation by the Laplace transform technique. The Lagrangian model is based on a generalized form of the Langevin equation. The first parameterisation, Degrazia et al. (2000), is based on Taylor’s statistical diffusion theory and the observed spectral properties, supposes a linear combination between shear and buoyancy turbulence. The second, Hanna (1982), is based on observed spectral properties from Minnesota Planetary Boundary Layer (PBL) observations and is widely used in pollutant dispersion models. Considering that these simulations are in the SBL, the analysis of the results shows a reasonably good agreement between the values computed by the models against the experimental ones for the two turbulence parameterisations.  相似文献   

Rapid tests to assess the antioxidant activity of Phaseolus vulgaris L. dry beans     

Heimler D  Vignolini P  Dini MG  Romani A 《Journal of agricultural and food chemistry》2005,53(8):3053-3056

The antiradical activity of dry beans was evaluated in order to assess the validity of this test and to correlate the results with those achieved with the Folin-Ciocalteu method and with a rapid spectrophotometric method for the analysis of total flavonoids. Four landraces (12 samples) of common beans (Phaseolus vulgaris L.), collected in two regions of Italy (Tuscany and Basilicata) in three different years, were analyzed. The EC50 values ranged from 39 to 2810 mg sample/mg 1,1-diphenyl-2-picrylhydrazil radical. The phenolic content of each sample was expressed as gallic acid equivalents; it changed from 1.17 to 4.40 mg/g. The flavonoid content, expressed as mg of (+)-catechin per g of dry seeds, ranged from 0.24 to 1.43 mg/g. The qualiquantitative composition of polyphenols has been also elucidated by means of high-performance liquid chromatography (HPLC)-diode array detection and HPLC/MS. These investigations showed that rapid tests can contribute to assessing the quality of functional food.  相似文献   

Purification and characterization of an N-acetylglucosamine-binding lectin from Koelreuteria paniculata seeds and its effect on the larval development of Callosobruchus maculatus (Coleoptera: Bruchidae) and Anagasta kuehniella (Lepidoptera: Pyralidae)     

Macedo ML  Damico DC  Freire Md  Toyama MH  Marangoni S  Novello JC 《Journal of agricultural and food chemistry》2003,51(10):2980-2986

This study describes the purification of an N-acetylglucosamine-binding lectin from Koelreuteria paniculata seeds and its effects on the larval development of Callobruchus maculatus and Anagasta kuehniella. The lectin (KpLec) was characterized and isolated by gel filtration, affinity column, and reverse phase chromatography. SDS-PAGE indicated that this lectin is a dimer composed of subunits of 22 and 44 kDa. The N terminus exhibited 40% similarity with Urtiga dioica agglutinin. KpLec was tested for anti-insect activity against C. maculatus and A. kuehniella. With regard to C. maculatus, an artificial diet containing 0.7 and 1% KpLec produced LD(50) and ED(50) value, respectively. However, for A. kuenhiella, an artificial diet containing 0.65% KpLec produced an LD(50), whereas 0.2% KpLec produced an ED(50). The transformation of genes coding for this lectin could be useful in the development of insect resistance in important agricultural crops.  相似文献