Box-Behnken设计优化黄精多糖酶法提取工艺     

杨德  薛淑静  黄蕾  郭鹏  高虹  史德芳  李露  陈晓春 《湖北农业科学》2017,56(16)

采用响应面法,以多糖提取率为评价指标,对酶解温度、酶添加量、酶解时间、料液比4个影响因素进行考察,优化酶法提取黄精多糖的提取工艺,另以Design-Expert 8.05软件对数据进行综合统计分析。结果表明,最优工艺为酶添加量3%、酶解温度54.8℃、酶解时间99 min、料液比1∶22.4(g/m L),经验证黄精多糖提取率为11.22%。利用Box-Behnken响应面法优选的黄精多糖酶法提取工艺稳定可行,可用于黄精多糖的提取。  相似文献   

Treatment of oral squamous cell carcinoma in a horse by surgical debulking followed by metronomic chemotherapy          下载免费PDF全文

R. Tornago  S. Sabattini  A. De Simoi  G. Bettini  T. Furlanello  V. Baldassarre  M. Cornali  M. Mazzucato  S. Carlin  F. Gentilini 《Equine Veterinary Education》2017,29(4):208-212

A 19‐year‐old Quarter Horse gelding presented with a 6‐week history of hypersalivation, halitosis and dysmasesis. Oral examination revealed retention of food and saliva and the presence of a raised, nodular, 6 × 7 cm, ulcerated mass on the dorsal surface of the tongue base. The mass was confirmed histologically as squamous cell carcinoma. Complete resection of the mass was not possible and surgical laser debulking was followed 15 days later by chemotherapy with a combination of meloxicam and cyclophosphamide in metronomic regimen. After one week, there was a significant improvement in clinical signs and food consumption returned to normal. Therapy was well tolerated with no alteration in haematological or urinalysis parameters. After 5 months of excellent life quality, the horse showed progressive difficulties in mastication and swallowing. Endoscopic examination showed extension of the tumour to all the aboral aspect of the tongue and, with the owner's consent, the patient was subjected to euthanasia. This is believed to be the first report on the combined use of meloxicam and cyclophosphamide in a metronomic fashion for management of an oral squamous cell carcinoma in a horse. Since metronomic therapy is less expensive than conventional chemotherapy, easily administrable and well tolerated, it should be considered as a possible treatment option for nonresectable equine malignant tumours.  相似文献   

Alternative method to facilitate resolution of paraphimosis after penile trauma in the horse          下载免费PDF全文

E. De Brouwer  T. Ribera  F. Climent  M. Prades 《Equine Veterinary Education》2017,29(12):655-658

Inflammation associated with traumatic injury to the penis is characterised by pain, heat and oedema. Ensuing penile swelling interferes with retraction of the penis into the prepuce, resulting in paraphimosis. This report describes the use of a 5 l capacity pressure infusion bag to reduce penile oedema in three horses. This technique provided complete resolution of oedema and associated paraphimosis in approximately 1 h. The use of the bag, along with an abdominoinguinal bandage, a plastic funnel suspensory device, or a penile repulsion device (Probang) to avoid recurrence, offers a novel treatment that may be performed with the horse sedated.  相似文献   

Preputial fibroma in a gelding          下载免费PDF全文

A. De Meyer  S. Vandenabeele  C. Ververs  A. Martens  K. Roels  V. De Lange  M. Hoogewijs  C. De Schauwer  J. Govaere 《Equine Veterinary Education》2017,29(1):7-9

An 11‐year‐old gelding presented with a large mass located in the dermis of his preputium that surrounded the penis completely. This mass was first noted years ago but did not increase in size. Histological examination diagnosed a fibroma. No treatment was initiated since no signs of discomfort were noticed and the gelding was performing at a high level in competitive showjumping. Next to skin tumours, tumours of the penis and preputium are most common. Most frequently, squamous cell carcinomas and sarcomas are diagnosed. Although fibromas can develop anywhere in connective tissue, a preputial location of a fibroma in a gelding is rare. Differential diagnosis and possible treatment options are discussed.  相似文献   

Rifampicin treatment of canine pyoderma due to multidrug‐resistant meticillin‐resistant staphylococci: a retrospective study of 32 cases          下载免费PDF全文

Michela De Lucia  Mar Bardagi  Elisabetta Fabbri  Diana Ferreira  Lluis Ferrer  Fabia Scarampella  Giordana Zanna  Alessandra Fondati 《Veterinary dermatology》2017,28(2):171-e36

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Heart rate variability parameters in horses distinguish atrial fibrillation from sinus rhythm before and after successful electrical cardioversion          下载免费PDF全文

B. Broux  D. De Clercq  A. Decloedt  S. Ven  L. Vera  G. van Steenkiste  K. Mitchell  C. Schwarzwald  G. van Loon 《Equine veterinary journal》2017,49(6):723-728

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Isolation,proliferation and characterization of endometrial canine stem cells          下载免费PDF全文

V De Cesaris  S Grolli  C Bresciani  V Conti  G Basini  E Parmigiani  E Bigliardi 《Reproduction in domestic animals》2017,52(2):235-242

In the last decade, progenitor cells isolated from dissociated endometrial tissue have been the subject of many studies in several animal species. Recently, endometrial cells showing characteristics of mesenchymal stem cells (MSC) have been demonstrated in human, pig and cow uterine tissue samples. The aim of this study was the isolation and characterization of stromal cells from the endometrium of healthy bitches, a tissue that after elective surgery is routinely discarded. Multipotent stromal cells could be isolated from all bitches enrolled in the study (n = 7). The multipotency of cells was demonstrated by their capacity to differentiate into adipocytic, osteocytic and chondrocytic lineages. Clonogenicity and cell proliferation ability were also tested. Furthermore, gene expression analysis by RT‐PCR was used to compare the expression of a set of genes (CD44, CD29, CD34, CD45, CD90, CD13, CD133, CD73, CD31 CD105, Oct4) with adipose tissue‐derived MSC. Stromal cells isolated from uterine endometrium showed similar morphology, ability of subculture and plasticity, and also expressed a panel of genes comparable with adipose tissue‐derived MSC. These data suggest that endometrial stromal cells fulfil the basic criteria proposed by the “Mesenchymal and Tissue Stem Cell Committee of the International Society for Cellular Therapy” for the identification of mesenchymal stem cells. Although endometrial mesenchymal stem cells (EnMSC) showed a lower replicative ability in comparison with adipose tissue‐derived MSC, they could be considered a cell therapeutic agent alternative to adipose tissue or bone marrow‐derived MSC in dog.  相似文献   

Prevention of intestinal parasites in a tri‐border area of Latin America: Children perceptions and an integral health education strategy          下载免费PDF全文

M. R. Rivero  M. M. Salas  R. Valente  M. J. Nores  C. De Angelo  J. Arrabal  S. Costa  O. D. Salomón 《Zoonoses and public health》2017,64(8):673-683

To investigate knowledge of school‐aged children and their perception on intestinal parasites, and to assess knowledge reconstruction on prevention practices after specific training in the subject. We performed an activity package that included the analysis of children's drawings of intestinal parasites, and information and communication technologies (ITCs) to transfer knowledge about these pathogens and prevention measures. Retrieval learning activities were performed to fixation of general and specific prevention and control measures.Overall, we found that there is a knowledge gap in many aspects of parasite biology and ecology, and therefore on the risk of infection and acquisition mechanisms. After ITCs, the children improved their knowledge over non‐trained children.The approaches used to transfer knowledge and for learning, fixation were valuable tools for incorporating changes in misconceptions and in the deep‐rooted habits that favour entero‐parasitic diseases. This has important implications for the specific design of future education materials and campaigns. Understanding of perceptions helps to provide justifications and knowledge to achieve changes in unhealthy habits, and it constitutes the basis for the transformation of many risky practices.  相似文献   

Suicide Attempt by Ingestion of Rotenone-Containing Plant Extracts in French Polynesia: A Case Report     

R. Torrents  B. Domangé  C. Schmitt  A. Boulamery  L. De Haro  N. Simon 《The Forestry Chronicle》2017,28(3):278-279

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Chronic cork oak decline and water status: new insights     

Constança S. P. Camilo-Alves  Margarida Vaz  M. Ivone Esteves Da Clara  Nuno Manuel De Almeida Ribeiro 《New Forests》2017,48(6):753-772

Chronic decline and Sudden death are two syndromes of cork oak (Quercus suber) dieback. Mortality is associated with water stress, but underlying physiological mechanisms are poorly understood. Here, we investigated the physiological performance of declining trees during the summer drought. Leaf water potential, gas-exchange, fluorescence of photosystem II and leaf and root starch concentration were compared in healthy (asymptomatic) and declining trees. Low annual cork increment in declining trees indicated tree decline for several years. All trees showed similar water status in spring. In summer, declining trees showed lower predawn leaf water potential (?2.0 vs. ?0.8 MPa), but unexpectedly higher midday leaf water potential than healthy trees (?2.8 vs. ?3.3 MPa). The higher midday water potential was linked to by means of strongly reduced stomatal conductance and, consequently, transpiration. This study is pioneer showing that declining trees had high midday water potential. A tendency for lower sap flow driving force (the difference between predawn and midday water potential) in declining trees was also associated with reduced photosynthesis, suggesting that chronic dieback may be associated with low carbon uptake. However, starch in roots and leaves was very low and not correlated to the health status of trees. Declining trees showed lower water-use efficiency and non-photochemical quenching in summer, indicating less resistance to drought. Contrarily to chronic decline, one tree that underwent sudden death presented predawn leaf water potential below the cavitation threshold.  相似文献