4个豆科牧草在干热河谷生态芒果园的应用研究     

张德  龙会英 《草地学报》2017,25(3)

为筛选出适宜在干热河谷生态果园种植的豆科牧草。本文设计牧草种类和翻压方式两水平因素,牧草种类是提那罗爪哇大豆(Glycine wightii(Wight and Arn.)Verdcourt‘Tinaroo’)、热研5号柱花草(Stylosanthes giuanensis‘Reyan No.5’)、大翼豆(Macroptilium atropurpureum(DC)Urb)和铺地木蓝(Indigofera endecaphylla Jacq.)4个牧草,翻压方式为翻压与不翻压,观测牧草草产量、地表温湿度、土壤湿度、土壤理化性状变化量;翻压方式观测翻压牧草前后土壤理化性状的变化。结果表明:在芒果园内种植提那罗爪哇大豆在改善果园土壤微环境及作为绿肥翻压提高土壤肥力方面均高于其他3个牧草;大翼豆地上生物量最高,年均干草产量为7 470.463kg·hm-2;热研5号柱花草枯枝落叶量最高,年均3 183.00kg·hm-2。种植牧草小区在调节地表温度及保持土壤水分效果高于未种植区,柱花草在调节地表温度及保持土壤水分的效果高于其他牧草。经济效益与草产量一致。  相似文献   

烟草丛顶病毒RNA依赖RNA聚合酶(RdRp)介导的体外复制体系     

逯晓明  于成明  王国鲁  王德亚  耿国伟  刘珊珊  师科荣  原雪峰 《植物病理学报》2017,47(6):790-796

通过重叠PCR扩增得到烟草丛顶病毒(Tobacco bushy top virus,TBTV)中国分离物RdRp的编码序列,构建以pMALC2X为基础载体的原核表达载体pMAL-TB-RdRp。0.5 mM IPTG诱导可特异性表达分子量约为120 kDa的MBP-RdRp融合蛋白。温度梯度实验显示,18℃下诱导表达的MBP-RdRp融合蛋白的可溶性比例较高,约17%;经亲和层析纯化的MBPRdRp可特异性识别TBTV正链和负链的3'末端序列,催化体外复制;对正负链的3'末端的体外复制效率存在差别,识别负链3'末端的体外复制效率明显高于正链3'末端。本研究创建的TBTV RdRp介导的体外复制体系为进一步研究TBTV基因组复制调控奠定了基础。  相似文献   

New invasion of <Emphasis Type="Italic">Bemisia tabaci</Emphasis> Mediterranean species in Brazil associated to ornamental plants     

Letícia Aparecida de Moraes  Julio Massaharu Marubayashi  Valdir Atsushi Yuki  Murad Ghanim  Vinicius Henrique Bello  Bruno Rossitto De Marchi  Leonardo da Fonseca Barbosa  Laura M. Boykin  Renate Krause-Sakate  Marcelo Agenor Pavan 《Phytoparasitica》2017,45(4):517-525

In Brazil, the first major invasion event of Bemisia tabaci was that of Middle East–Asia Minor 1 (MEAM1) species, formerly termed as B biotype, which commenced in the 1990s mainly by ornamental plants in São Paulo State. More than two decades after this invasion, the presence of the Mediterranean (MED) species of B. tabaci, formerly Q biotype, was reported in Rio Grande do Sul, the southernmost State of Brazil, and now in São Paulo and Paraná States, in southeastern Brazil. Specimens of whiteflies collected from commercial begonia, hydrangea, petunia and poinsettia greenhouses in São Paulo, and also from begonias and poinsettias collected in flower shops in Paraná, were all identified as belonging to MED species. Furthermore, the secondary endosymbionts Arsenophonus, Hamiltonella and Rickettsia of MED from São Paulo and Paraná were detected by PCR and their presence confirmed by sequencing and FISH analysis, and those results differed from MED detected in Rio Grande do Sul that harbored only Hamiltonella and Cardinium. Our results suggest a new MED invasion into Brazil and is associated with ornamental plants. The two MED populations are genetically different and suggest that they are separate invasions.  相似文献   

Fast and sensitive on-site isothermal assay (LAMP) for diagnosis and detection of three fruit tree phytoplasmas     

K. De Jonghe  I. De Roo  M. Maes 《European journal of plant pathology / European Foundation for Plant Pathology》2017,147(4):749-759

Over the years, real-time PCR outflanked endpoint PCR in phytopathogen diagnostics, mainly because of the increase in sensitivity and timesaving aspects of the technique. However, a time consuming 16S rRNA-based nested PCR method is still the gold standard for phytoplasma diagnosis. This is also the case for phytoplasma detection in Malus, Pyrus and Prunus, the three main host plants of apple proliferation (AP), pear decline (PD) and European stone fruit yellows (ESFY) phytoplasma, respectively. The last decade, loop-mediated isothermal amplification (LAMP) (Notomi et al. 2000) is gaining a lot in significance and is also for phytoplasmas expected to become a widely used reliable diagnostic tool. High specificity and sensitivity which also requires a less stringent need for DNA purification, and the short analysis time and the limited equipment requirements makes the LAMP method a fast and affordable alternative with great point-of-care diagnostic potential. In this paper, we present a LAMP primer set for the ribosomal group 16SrX, containing the important fruit tree phytoplasmas AP, PD and ESFY. The primers were developed and validated for fast and sensitive detection and general use for diagnosis. We foresee that the LAMP technique will also have its application in on-site diagnosis of the fruit tree phytoplasmas during inspections and surveys.  相似文献   

Evaluation of a decision support strategy for the control of powdery mildew,Erysiphe necator (Schw.) Burr., in grapevine in the central region of Chile          下载免费PDF全文

Héctor Valdés‐Gómez  Miguel Araya‐Alman  Carolina Pañitrur‐De la Fuente  Nicolás Verdugo‐Vásquez  Mauricio Lolas  César Acevedo‐Opazo  Christian Gary  Agnès Calonnec 《Pest management science》2017,73(9):1813-1821

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Inoculative release strategies of Macrolophus pygmaeus Rambur (Hemiptera: Miridae) in tomato crops: population dynamics and dispersal     

Moerkens  Rob  Berckmoes  Els  Van Damme  Veerle  Wittemans  Lieve  Tirry  Luc  Casteels  Hans  De Clercq  Patrick  De Vis  Raf 《植物病害和植物保护杂志》2017,124(3):295-303

Journal of Plant Diseases and Protection - Macrolophus pygmaeus Rambur (Hemiptera: Miridae) is a key biological control agent in greenhouse tomato crops. In the present study, we describe the...  相似文献   

Box-Behnken设计优化黄精多糖酶法提取工艺     

杨德  薛淑静  黄蕾  郭鹏  高虹  史德芳  李露  陈晓春 《湖北农业科学》2017,56(16)

采用响应面法,以多糖提取率为评价指标,对酶解温度、酶添加量、酶解时间、料液比4个影响因素进行考察,优化酶法提取黄精多糖的提取工艺,另以Design-Expert 8.05软件对数据进行综合统计分析。结果表明,最优工艺为酶添加量3%、酶解温度54.8℃、酶解时间99 min、料液比1∶22.4(g/m L),经验证黄精多糖提取率为11.22%。利用Box-Behnken响应面法优选的黄精多糖酶法提取工艺稳定可行,可用于黄精多糖的提取。  相似文献   

Treatment of oral squamous cell carcinoma in a horse by surgical debulking followed by metronomic chemotherapy          下载免费PDF全文

R. Tornago  S. Sabattini  A. De Simoi  G. Bettini  T. Furlanello  V. Baldassarre  M. Cornali  M. Mazzucato  S. Carlin  F. Gentilini 《Equine Veterinary Education》2017,29(4):208-212

A 19‐year‐old Quarter Horse gelding presented with a 6‐week history of hypersalivation, halitosis and dysmasesis. Oral examination revealed retention of food and saliva and the presence of a raised, nodular, 6 × 7 cm, ulcerated mass on the dorsal surface of the tongue base. The mass was confirmed histologically as squamous cell carcinoma. Complete resection of the mass was not possible and surgical laser debulking was followed 15 days later by chemotherapy with a combination of meloxicam and cyclophosphamide in metronomic regimen. After one week, there was a significant improvement in clinical signs and food consumption returned to normal. Therapy was well tolerated with no alteration in haematological or urinalysis parameters. After 5 months of excellent life quality, the horse showed progressive difficulties in mastication and swallowing. Endoscopic examination showed extension of the tumour to all the aboral aspect of the tongue and, with the owner's consent, the patient was subjected to euthanasia. This is believed to be the first report on the combined use of meloxicam and cyclophosphamide in a metronomic fashion for management of an oral squamous cell carcinoma in a horse. Since metronomic therapy is less expensive than conventional chemotherapy, easily administrable and well tolerated, it should be considered as a possible treatment option for nonresectable equine malignant tumours.  相似文献   

Alternative method to facilitate resolution of paraphimosis after penile trauma in the horse          下载免费PDF全文

E. De Brouwer  T. Ribera  F. Climent  M. Prades 《Equine Veterinary Education》2017,29(12):655-658

Inflammation associated with traumatic injury to the penis is characterised by pain, heat and oedema. Ensuing penile swelling interferes with retraction of the penis into the prepuce, resulting in paraphimosis. This report describes the use of a 5 l capacity pressure infusion bag to reduce penile oedema in three horses. This technique provided complete resolution of oedema and associated paraphimosis in approximately 1 h. The use of the bag, along with an abdominoinguinal bandage, a plastic funnel suspensory device, or a penile repulsion device (Probang) to avoid recurrence, offers a novel treatment that may be performed with the horse sedated.  相似文献   

Preputial fibroma in a gelding          下载免费PDF全文

A. De Meyer  S. Vandenabeele  C. Ververs  A. Martens  K. Roels  V. De Lange  M. Hoogewijs  C. De Schauwer  J. Govaere 《Equine Veterinary Education》2017,29(1):7-9

An 11‐year‐old gelding presented with a large mass located in the dermis of his preputium that surrounded the penis completely. This mass was first noted years ago but did not increase in size. Histological examination diagnosed a fibroma. No treatment was initiated since no signs of discomfort were noticed and the gelding was performing at a high level in competitive showjumping. Next to skin tumours, tumours of the penis and preputium are most common. Most frequently, squamous cell carcinomas and sarcomas are diagnosed. Although fibromas can develop anywhere in connective tissue, a preputial location of a fibroma in a gelding is rare. Differential diagnosis and possible treatment options are discussed.  相似文献