<Emphasis Type="Italic">Abelmoschus angulosus</Emphasis> var. <Emphasis Type="Italic">mahendragiriensis</Emphasis> (Malvaceae): a new taxonomic variety of wild okra from Eastern Ghats of India     

R. C. Misra  D. R. Pani  L. K. Bharathi  S. P. Ahlawat 《Genetic Resources and Crop Evolution》2018,65(3):993-1002

Abelmoschus angulosus var. mahendragiriensis R.C.Misra var. nov., from a gorge in the Mahendragiri hills of Odisha in the Eastern Ghats is described and illustrated. Information about the type specimen, illustration, taxonomic affinities, distribution and ecology and field photographs showing vegetative and floral parts of the new variety along with an identification key with diagnostic characters in relation to other known varieties are provided.  相似文献   

Phenetic and genetic diversity in Indian <Emphasis Type="Italic">Luffa</Emphasis> (Cucurbitaceae) inferred from morphometric,ISSR and DAMD markers     

Sujata Misra  Awadhesh Kumar Srivastava  Sushma Verma  Sudhakar Pandey  Surendra Singh Bargali  Tikam Singh Rana  K. Narayanan Nair 《Genetic Resources and Crop Evolution》2017,64(5):995-1010

Genetic variations and relationships among cultivated and wild genotypes of five taxa of Indian Luffa were examined using inter simple sequence repeats (ISSR), directed amplification of minisatellite DNA (DAMD) markers and morphological characterization. Morphometric evaluation of 21 discrete characters in 51 representative accessions segregated the five taxa of Luffa in three main clusters: the two wild species (L. echinata, L. graveolens) in the first, and the cultivated L. aegyptiaca (smooth gourd) and L. acutangula (ridged gourd)/L. hermaphrodita (Hermaphrodite luffa) in the second and third clusters, respectively. Cumulative data analysis of 15 ISSR and seven DAMD markers revealed high percentage polymorphism (97.67 %), moderate genetic distance (0.06–0.72, avg. 0.51), and low heterozygosity and Shannon index values (H = 0.15; I = 0.22) across all the 76 genotypes assayed. A UPGMA dendrogram, based on the combined marker data, resolved the five taxa in two main clusters with high bootstrap support. The morphological and molecular trees showed incongruence in the number of main clusters resolved and in the disposition of the wild and cultivated taxa in different sub-clusters. The cluster analyses and PCoA plots revealed a nested grouping of the hermaphrodite luffa within the ridge gourd group. The Bayesian STRUCTURE analysis identified three genetic clusters for the five assumed taxa. Outcrossing test revealed a mixed mating system in Indian Luffa. This is the first ever report on the mating system in Luffa using molecular markers. The study also demonstrates the utility of using more than one DNA marker in the assessment of molecular diversity in a widely cultivated crop genus like Luffa with a narrow genetic base.  相似文献   

Alkaline phosphatase activity of bursa of Fabricius in White Leghorn pullets     

M S Misra 《The Indian veterinary journal》1970,47(4):312-314

  相似文献   

Activation of bovine monocytes and neutrophils by the Bb fragment of complement factor B: demonstration by the uptake of 3H-deoxyglucose.   总被引：2，自引：2，他引：0       下载免费PDF全文

M S Sethi  H Tabel    V Misra 《Canadian journal of veterinary research》1990,54(1):106-112

The Bb fragment is the enzymatically active split product of bovine complement factor B. The Bb fragment was obtained after zymosan treatment of fresh bovine serum and fractionation of the treated serum, first over diethylaminoethyl-Sephacel and then over an affinity column made up of monoclonal antibody to bovine Bb, coupled to cyanogen-bromide-activated Sepharose. Purified Bb has a molecular weight of 64,000, as determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The ability of purified Bb to activate phagocytes was assessed. The activation assay was based on the principle that the primary source of energy for the phagocytes is obtained from glucose. 3H-deoxyglucose, a nonmetabolizable analogue of glucose, was used to obtain the quantitative measurement of the activation process. The activation by Bb was shown by the uptake of the labelled deoxyglucose in the phagocytic cells and was comparable to the activation caused by phorbol myristate acetate and N-formyl-L-methionyl-L-leucyl-L-phenylalanine, run in parallel. These data showed that fragment Bb activates bovine monocytes and neutrophils and also suggested that, when generated after complement activation, Bb may stimulate monocytes and neutrophils for enhanced phagocytosis.  相似文献   

Studies on the adsorption and release of copper by soils     

S. G. Misra  R. C. Tiwari 《Soil Science and Plant Nutrition》2013,59(3):1-4

In a previous communication we¹⁾ reported the adsorption and subsequent release of adsorbed Cu⁺⁺ by two soils and a compost sample using fairly high concentrations of Cu⁺⁺ ions in the form of copper sulphate solution.  相似文献   

Treatment of wheat straw using tannase and white-rot fungus to improve feed utilization by ruminants   总被引：1，自引：0，他引：1  

Shailendra Raghuwanshi  Swati Misra  Rajendra Kumar Saxena 《畜牧与生物技术杂志(英文版)》2014,(2):222-229

Background： Current research to enrich cattle feed has primarily focused on treatment using white rot fungi, while there are scarce reports using the enzyme tannase, which is discussed only in reviews or in the form of a hypothesis. In this context, the aim of the present study was to evaluate the effect of tannase on wheat straw （WS） and also the effect of lyophilized tannase at concentrations of 0.1%, 0.2%, and 0.3% （w/w） on WS followed by fermentation with Ganodermo sp. for 10 d and compared in relation to biochemical parameters, crude protein （CP） content, and nutritional value by calculating the C/N ratio in order to improve the nutritional value of cattle feed. Results： Penicillium charlesii, a tannase-producing microorganism, produced 61.4 IU/mL of tannase in 54 h when 2% （w/v） tannic acid （TA） was initially used as a substrate in medium containing （% w/v） sucrose （1.0）, NaNO3 （1.0）, and MgSO4 （0.08 pH, 5.0） in a 300-L fermentor （working volume 220 L）, and concomitantly fed with 1.0% （w/v） TA after 24 h. The yield of partially purified and lyophilized tannase was 5.8 IU/mg. The tannin-free myco-straw at 0.1% （w/w） tannase showed 37.8% （w/w） lignin degradation with only a 20.4% （w/w） decrease in cellulose content and the in vitro feed digestibility was 32.2%. An increase in CP content （up to 1.28-fold） along with a lower C/N ratio of 25.0%, as compared to myco-straw, was obtained. Conclusions： The use of tannin-free myco-straw has potential to improve the nutritional content of cattle feed. This biological treatment process was safe, eco-friendly, easy to perform, and was less expensive as compared to other treatment methods.  相似文献   

Amino acid profile of winged bean (Psophocarpus tetragonolobus (L.) DC): a rich source of vegetable protein     

Dhan Prakash  P. N. Misra  P. S. Misra 《Plant foods for human nutrition (Dordrecht, Netherlands)》1987,37(3):261-264

Twenty one cultivars of winged bean (17 of Indian origin and 4 exotic strains obtained from Sri Lanka) were cultivated on marginal (alkaline) soils in the sub-tropics and evaluated for their food value. Whereas the seeds showed wide variations in total protein content (38.1 to 45 per cent), the amino acid compositions were in close proximity to those of soybean. Nevertheless, some strains of winged bean registered higher levels of certain amino acids as compared to soybean.  相似文献