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501.
Global efforts to reduce unwanted catches have led to the development of a vast array of by‐catch reduction devices (BRDs), in particular for mixed trawl fisheries. Some of these BRDs could likely benefit from being combined. However, the number of possible combinations would be prohibitive to be tested experimentally. Therefore, in this study we propose a meta‐analytical approach that combines the data available on BRDs tested independently in a fishery and predict the theoretical selectivity of all possible combinations of those devices. This allows to identify promising BRD combinations, worth experimental investigation and flexible trawl configurations, where the selectivity can be substantially modified by adding or removing one BRD, thus aiding fishermen in adapting to high variability in catch composition and quota availability. To illustrate the approach, we used BRDs developed for the well‐studied Nephrops (Nephrops norvegicus, Nephropidae) directed mixed trawl fishery in the Skagerrak and Kattegat seas. We predicted the selectivity of 100 BRD combinations for Nephrops, cod (Gadus morhua, Gadidae) and haddock (Melanogrammus aeglefinus, Gadidae), compared them in terms of absolute selectivity and performance under realistic catch scenarios, from both single‐ and multispecies perspectives, and identified 15 BRD combinations that could be worth future experimental investigation. The meta‐analytical approach makes best use of existing knowledge and leads to new insights about the potential for improvement and flexibility in trawl selectivity. This could benefit a variety of mixed trawl fisheries and help developing a new generation of more flexible gears, with multiple BRDs integrated in their structure.  相似文献   
502.
Chile is currently the second largest producer of farmed salmon worldwide, but Flavobacterium psychrophilum, as one of the most detrimental pathogens, is responsible for major losses during the freshwater culturing step in salmonid fish farms. An antigenic study conducted 10 years ago reported four serological groups using 20 F. psychrophilum Chilean strains. To reduce disease outbreaks and to develop vaccine candidates, antigenic knowledge needs to be regularly updated using a significant number of additional recent F. psychrophilum isolates. The present study aimed at investigating the serological diversity of 118 F. psychrophilum isolates collected between 2006 and 2018 from farmed Atlantic salmon (Salmo salar), rainbow trout (Oncorhynchus mykiss) and coho salmon (Oncorhynchus kisutch). The current study supports an expansion of the known antigenic groups in Chile from 4 to 14. However, the use of the slide-agglutination technique for serotyping is costly, is labour-intensive and requires significant technical expertise. Addressing these points, the mPCR-based procedure was a very useful tool for serotyping the collected Chilean F. psychrophilum isolates. This technique revealed the presence of diverse mPCR serotypes (i.e. types 0, 1, 2 and 4). Therefore, mPCR should be employed to select the bacterial strain(s) for vaccine development and to conduct follow-up, selective breeding or epidemiological surveillance in Chilean fish farms. Given the presented findings, changes to Chilean fish-farming practices are vital for ensuring the continued productivity and well-being of farmed salmonids.  相似文献   
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凡纳滨对虾养殖塘叶绿素a与水质因子的多元回归分析   总被引:3,自引:0,他引:3  
2009年4-9月期间,对上海市奉贤区某凡纳滨对虾养殖场22个养殖池塘水体叶绿素a、水温、pH、溶解氧、透明度、悬浮物(SS)、总有机碳(TOC)、五日生化需氧量(BOD5)、高锰酸盐指数(CODMn)等15项水质因子进行测定。取164组测定数据,进行描述性统计,分析叶绿素a与各项因子的相关性系数。分析结果显示,与叶绿素a呈极显著线性正相关的水质因子为SS、TOC、BOD5、CODMn、TN、TP;呈显著正相关的为DO;而叶绿素a与透明度呈极显著线性负相关,与PO3-4-P呈显著线性负相关;与水温、pH、NO-2-N、 NO-3-N、NH3-N则未呈现显著相关性。根据多元线性回归选择自变量的原则,选择了TOC、TN、PO3-4-P和TP4项水质因子,建立了叶绿素a与4项水质因子的逐步回归模型:Chl.a =-0.054 5+0.0034 9 TOC+0.015 3 TN-0.418 PO3-4-P+0.276 TP(r=0.715 5)。利用偏回归系数检验各水质因子对叶绿素a的影响,结果表明,对叶绿素a影响从大到小依次是TP、TOC、PO3-4-P和TN。研究结果对进一步探讨养殖池塘生态系统的变化规律及水环境质量保护提供了依据。  相似文献   
507.
Hantavirus Pulmonary Syndrome (HPS) is an emerging infectious disease caused by orthohantaviruses in the Americas. In Argentina, since 1995, several reservoirs and virus variants have been described, but the northeastern and central endemic zones in the country include an area without human or rodent infections, despite sharing rodent species with areas with that disease. The aim of this study was to search for orthohantavirus in rodent communities that inhabit this area, which borders two endemic areas of HPS. Small rodents were captured in June of 2022 through a total effort of 644 trap nights distributed in five grids located in the Iberá National Park, Corrientes, Northeastern Argentina. All rodents were sexed, weighed, and the species was recorded. Blood samples were extracted to detect ANDV-specific immunoglobulin G (IgG), and to extract the RNA virus. Trimmed sequences were mapped against reference sequences from GenBank. We captured a total of 36 Oligoryzomys flavescens and 15 Oxymycterus rufus. We detected the O. flavescens species infected with Lechiguanas orthohantavirus in the camping area of the National Park. A nucleotide comparison with previously published sequences shows a 98.34% similarity to the virus obtained from a human case of HPS reported in the adjacent Misiones province. This study demonstrated, for the first time, that O. flavescens is a host of the Lechiguanas orthohantavirus in this zone and contributes to closing information gaps on the distribution of orthohantavirus in Argentina. Additionally, the high similarity with the hantavirus found in the human case of Misiones suggests that the reservoir in that province would also be O. flavescens (not previously confirmed). This information permits us to focus on the preventive measurements to protect the human population.  相似文献   
508.
Pig slurries are widely used on calcareous soils in European rainfed systems. Here we assess their impact on the amount of soil organic carbon (SOC) and on the composition of humic-type substances (HTS). Seven doses of slurry (five from fattening pigs and two from sows) ranging from 1.0 to 4.8 Mg ha−1 yr−1 of organic matter were evaluated after a period of 12 years and compared with mineral fertilizer treatment. At the end of the last annual cropping season (September), SOC was quantified, and HTS were isolated by alkaline extraction followed by acid precipitation, and studied by visible spectroscopy (800–400 nm) and Fourier-transformed infrared spectroscopy (4000–400 cm−1). Following the trend in the slurry organic matter applied rates, SOC increased from 9.5 g C kg−1 (mineral treatment) to 13.8 g C kg−1. This SOC increase was equivalent to c. 25.4% of the slurry organic carbon applied. The incorporation of aliphatic structures, mainly polyalkyl, from slurries into the HTS tends to modify the composition of the soil organic matter (SOM), which is reflected in a decrease in the intensity of FT-IR peaks related to aromatic structures. Despite the trend of significant increase in SOC with fattening slurries, mainly from the organic matter rate of 1.6 Mg ha−1 yr−1 (c. 185 kg N ha−1), the composition of the HTS showed an important aliphatic enhancement. The FTIR results showed that using exclusively the relative intensities of specific peaks (alkyl, carboxyl, aromatic and amide groups) as variables for the discriminant analysis, it is possible to identify HA between different groups of soils treated with progressive levels of slurry. Although the new aliphatic components could be considered important to improve soil physical quality, after the incorporation of additional SOM, the spectroscopic characteristics of HTS in soils treated with slurries suggested a weak effect in long-term C sequestration, as the newly incorporated OC forms are not qualitatively similar to the presumably stable native SOM. These potential changes in SOC and SOM composition at field level are constrained by the maximum allowed N rates from organic origin in some agricultural systems.  相似文献   
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