This study aims to investigate the effects of essential oils (EOs), extracted from Thymus satureioides (TS) and Origanum majorana (OM), on Beni Arouss buck semen quality stored in skimmed milk at 4°C. EOs were extracted by hydro-distillation, and the chemical compounds were determined. Ejaculates were collected from six Beni Arouss bucks, once a week for 10 weeks, and they were pooled, divided into five equal aliquots and diluted to 400 × 106 sperm/ml with skimmed milk supplemented with 0.01% of OM EO, 0.01% of TS EO, 0.05% of OM EO and 0.05% of TS EO. Non-supplemented skimmed milk was considered as a control. Semen motility, kinematic parameters, viability, abnormality, membrane integrity and lipid peroxidation were evaluated at 0, 4, 8, 24, 28, 32 and 48 hr of liquid storage at 4°C. The main EO components were carvacrol (31.7%), thymol (28.0%) and borneol (14.4%) for TS, and terpinene-4-ol (31.2%), γ-terpinene (17.4%) and α-terpinene (12.7%) for OM. The results highlighted a dose-dependent effect of TS and OM EOs on all semen quality parameters. 0.01% of both EOs had a beneficial effect on the sperm preservation stored at 4°C compared with control (p < .05) excepted for the straight-line velocity. The 0.05% EO addition had harmful effects during storage particularly for TS EO. In conclusion, 0.01% of TS and OM EOs are recommended to improve the Beni Arouss buck semen preservation at 4°C. 相似文献
Asparagus officinalis is the most economically important species of asparagus worldwide. Commercial cultivars of asparagus are susceptible to some biotic and abiotic stresses. Drought stress is one of the most common stresses in agricultural crops cultivation. The main purpose of the current study was to compare Iranian asparagus accessions drought tolerance rate with that of ‘Mary Washington’ commercial cultivar and introducing superior accessions in terms of dry resistance rate. A factorial experiment was laid out based on a completely randomized design with three replications. The factors were a) asparagus accession in 10 levels including nine wild accessions of Iranian asparagus and ‘Mary Washington’ commercial cultivar (as control) and b) drought stress in four levels including 0 (as control), 5, 10 and 15% (w/v polyethylene glycol). Results showed that highest values of percentage and speed of seed germination and lengths of radicle and plumule were recorded in Gazanak accession. The lowest values of radicle length were recorded in Chalaki and Shiraz accessions (6.19 and 8.01?mm, respectively). Calli of ‘Mary Washington’ cultivar under highest applied level of drought stress showed the lowest value of relative growth rate (11.53?mg/day). Cluster analysis based on assessed characteristics under different applied levels of drought stress, divided Iranian asparagus accessions into four groups at Euclidean distance of 2.0. In conclusion, obtained results showed that Iranian asparagus accessions especially Gazanak accession could be a good candidate to apply in asparagus breeding programs with aim of producing asparagus dry tolerant cultivars.
The properties of soils affected by salinity and processes involving degradation of soil structure have been partly recognized. However, the effects of saline and sodic conditions on mechanical and physical properties of soils have been studied to a lesser extent. In this research, the effects of electrical conductivity (EC) and sodium adsorption ratio (SAR) on soils possessing various amounts of organic matter were assessed under laboratory conditions. The soils contained a uniform clay type, predominantly Illite. The major difference of the soils was their amount of organic matter content. The treatments consisted of solutions with definite EC and SAR (two levels of EC: 0.5 and 4 dS/m and three levels of SAR: 0, 5 and 15). The amount of tensile strength was dependent on organic matter, EC, and SAR in a way that with the increase of SAR, the tensile strength decreased. In similar SAR, treatments with higher EC exhibited greater tensile strength. Also, the soils with higher organic matter showed greater tensile strength. The analysis of variance showed the significant difference (at 1%) between the mean of parameters analyzed (soil type, sampling depth, EC, and SAR). The order of averages of tensile strength were: permanent pasture (Agropyron elengatum)Festuca arusdinaceae)相似文献
A study was conducted to investigate the effects of dietary oligofructose (1, 2 and 3%) on the blood profiles of beluga (Huso huso) juveniles (18.77 ± 0.76 g) compared to fish fed an un-supplemented diet. After 7 weeks of feeding on the experimental diets,
haematological parameters, metabolic products (cholesterol, glucose and total protein) and serum enzymes (lactate dehydrogenase,
alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase) were measured. Compared to the control group
(0% oligofructose), dietary oligofructose had no effect on red blood cell counts (RBC), mean corpuscular volume (MCV), mean
cellular haemoglobin (MCH) or mean cell haemoglobin concentration (MCHC) (P > 0.05). However, haemoglobin (Hb) concentration, leucocyte (WBC) levels and the proportion of lymphocytes were significantly
higher (P > 0.05) in the 2% oligofructose fed fish than in the 3% oligofructose fed fish. Additionally, haematocrit (Hct) values (P = 0.049) and the proportion of lymphocytes (P ≤ 0.01) were significantly higher in the 2% oligofructose group than in the control group. Although serum glucose and total
protein remained unaffected, serum cholesterol was significantly lower in the 2% oligofructose group than in the control and
3% oligofructose group (P < 0.05). The results of the present study showed that oligofructose had no significant effects on serum lactate dehydrogenase,
alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase. These results indicate that fish blood profiles
could be affected by prebiotics, which should be taken into account in future studies. 相似文献
An eight‐week research was conducted to investigate the effects of single or combined administration of sodium propionate (Na‐P) and sodium acetate (Na‐A) on the performance of yellowfin seabream (Acanthopagrus latus) juveniles (6.5 ± 0.3 g). A plant protein (PP)‐rich diet was supplemented with sole or blends of organic acid salts (OAS) namely Na‐P and Na‐A to design six experimental feeds including control (without OAS), Na‐P5 (5 g/kg Na‐P), Na‐P10 (10 g/kg Na‐P), Na‐A5 (5 g/kg Na‐A), Na‐A10 (10 g/kg Na‐A) and Na‐P + A (5 g/kg Na‐P + 5 g/kg Na‐A). Except for Na‐A5 group, the other OAS‐supplemented treatments had higher growth and feed efficiency ratio than the control (p < .05). The inclusion of OAS in the experimental feeds pronouncedly enhanced plasma lysozyme and alternative complement pathway activities compared to the control. Furthermore, fish fed on the OAS‐supplemented diets had greater catalase and glutathione peroxidase activities in the liver than the control (p < .05). Total antioxidant capacity in the liver of fish fed on the OAS‐supplemented diet also was higher than the control. Fish fed on the OAS‐supplemented diets had higher pepsin, trypsin and lipase activities than the control. The insulin‐like growth factor 1 (IGF‐1) gene expression was remarkably down‐regulated in the liver of fish fed on the OAS‐supplemented diets compared to the control especially in groups fed on the Na‐P10 and Na‐A10 diets. The greatest IGF‐1 gene down‐regulation level in the gut was in fish fed on the Na‐P5 and Na‐P10 diets. The interleukine‐1β in the gut was remarkably up‐regulated in the control compared to the other groups (p < .05). The lactic acid bacterial colonies count in the gut of the control was lower than the OAS‐supplemented groups. Based on the findings of the present study, supplementing PP‐rich diets with 10 g/kg Na‐P or blends of Na‐P (5 g/kg) and Na‐A (5 g/kg) beneficially alleviated inflammatory responses and improved immune parameters and digestive capacity in yellowfin seabream juveniles. 相似文献