Effects of cryopreservation on stallion sperm protamine messenger RNAs     

Ali Kadivar  Naser Shams Esfandabadi  Ehsan Dehghani Nazhvani  Abolfazl Shirazi  Ebrahim Ahmadi 《Reproduction in domestic animals》2020,55(3):274-282

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Standardization of the outbred BALB/c mice as a suitable animal model for <Emphasis Type="BoldItalic">Besnoitia caprae</Emphasis> studies     

Namazi F  Namavari MM  Rahimi E  Lotfi M  Tahamtan Y 《Veterinary research communications》2011,35(7):425-431

The Apicomplexan parasite Besnoitia caprae infects wild and domestic goats. Knowledge on Besnoitia caprae specially an optimized animal model is sparse. Experimental infections with tachyzoites of BC-Pars obtained from BALB/c mice were conducted in outbred mice to determine the infectivity and LD50 of Besnoitia caprae. Six groups of five mice were intraperitoneally infected with 12.5 × 10³, 25 × 10³, 5 × 10⁴, 1 × 10⁵ and 2 × 10⁵ tachyzoites and a control inoculum of DMEM, respectively. Although morbidity and mortality were observed in all groups, two mice in the 12.5 × 10³ group showed alopecia and skin lesions on 60 days post-infection (DPI). Histopathological and molecular examination of skins confirmed B. caprae infection. The LD₅₀ was calculated as 25 × 10^3.2 tachyzoites per mouse. The results indicate that outbred BALB/c mice can be used as a suitable model of besnoitiosis and to screen candidate treatments and to test the efficacy of vaccines for besnoitiosis.  相似文献   

A common variant in the FTO gene is associated with body mass index and predisposes to childhood and adult obesity   总被引：2，自引：0，他引：2  

Frayling TM  Timpson NJ  Weedon MN  Zeggini E  Freathy RM  Lindgren CM  Perry JR  Elliott KS  Lango H  Rayner NW  Shields B  Harries LW  Barrett JC  Ellard S  Groves CJ  Knight B  Patch AM  Ness AR  Ebrahim S  Lawlor DA  Ring SM  Ben-Shlomo Y  Jarvelin MR  Sovio U  Bennett AJ  Melzer D  Ferrucci L  Loos RJ  Barroso I  Wareham NJ  Karpe F  Owen KR  Cardon LR  Walker M  Hitman GA  Palmer CN  Doney AS  Morris AD  Smith GD  Hattersley AT  McCarthy MI 《Science (New York, N.Y.)》2007,316(5826):889-894

Obesity is a serious international health problem that increases the risk of several common diseases. The genetic factors predisposing to obesity are poorly understood. A genome-wide search for type 2 diabetes-susceptibility genes identified a common variant in the FTO (fat mass and obesity associated) gene that predisposes to diabetes through an effect on body mass index (BMI). An additive association of the variant with BMI was replicated in 13 cohorts with 38,759 participants. The 16% of adults who are homozygous for the risk allele weighed about 3 kilograms more and had 1.67-fold increased odds of obesity when compared with those not inheriting a risk allele. This association was observed from age 7 years upward and reflects a specific increase in fat mass.  相似文献   

Impact of Animal Manure on Soil Chemistry,Mineral Nutrients,Yield, and Fruit Quality in ‘Golden Delicious’ Apple     

Mohammad E. Amiri  Esmaeil Fallahi 《Journal of plant nutrition》2013,36(4):610-617

ABSTRACT

Incorporating deep litter cow and deep litter poultry manures with the top 30-cm soil improved orchard soil chemistry, including nutrient availability, soil organic matter, electrical conductivity (EC), pH, cation exchange capacity (CEC) and biological activity in a ‘Golden Delicious’ apple (Malus domestica Bork) orchard in Zanjan, Iran. Application of deep litter cow manure at 30 t ha^?1 or deep litter poultry manure at 10 t ha^?1 resulted in a higher rate of nitrogen (N) release, and thus increased yield and fruit size, but decreased fruit color. The least leaf minerals were found in the untreated control trees. The control trees showed minor symptoms of N, iron (Fe), and magnesium (Mg) deficiencies during the following season. Positive correlation existed between the rate of manure applied and the content of soil organic matter (OM). The deep litter poultry manure at 10 t ha^?1 increased the soil K, Mg, calcium (Ca), ammonium-N, and EC levels.  相似文献   

CRITICAL DEFICIENCY LEVEL OF ZINC FOR CORN ON CALCAREOUS SALT-AFFECTED SOILS IN CENTRAL IRAN     

A. H. Khoshgoftarmansh  H. Kheir Abadi  Z. Khanmohammadi  F. Amiri Sararoudi  M. Barzin  A. R. Pilevar Shahri 《Journal of plant nutrition》2013,36(12):1806-1818

A greenhouse experiment with 11 soil series and two zinc (Zn) rates (0 and 15 mg Zn kg^?1 as zinc sulfate) was performed to determine critical deficiency level of Zn for corn (Zea mays L.) on calcareous salt-affected soils in central Iran. In addition, the most important soil properties affecting Zn phytoavailability were determined. Critical Zn deficiency levels were determined using the Cate-Nelson and Mitscherlich procedures. In most soils, application of Zn increased the dry matter yield, and Zn concentration and content in the shoot and root of corn. A positive correlation was observed between the soil electrical conductivity (EC) with Zn concentration in shoots, roots and whole plant while shoot Zn content was negatively correlated with buffer capacity of Zn in soil. Critical deficiency levels of Zn in soil for corn based on the Cate-Nelson and Mitscherlich method were 1.35 and 1.23 mg kg^?1 for diethylenetriaminepentaacetic acid (DTPA)-extracted soil Zn, respectively.  相似文献   

Evaluation of water schemes for peanut,using CSM-CROPGRO-Peanut model     

E. Amiri  A. Abdzad Gohari  A. Mianabadi 《Archives of Agronomy and Soil Science》2013,59(10):1439-1453

Drought stress is an important factor limiting the yield potential of peanut. In order to determine the effect of different irrigation scenarios on peanut production, field experiments were conducted in 2011 and 2012 growing seasons using factorial design with three replicates. On the other hand, the crop simulation models can be useful to predict crop yields and to investigate the impact of drought stress on plant growth and development. In this study, the Cropping System Model–Crop Growth (CSM-CROPGRO)-Peanut model was employed for the simulation of seed yield, pod yield, biomass, soil water balance components and water productivity for peanut in Astaneh-Ashrafiyeh, Iran. Results showed that the model was able to reasonably simulate seed yield, pod yield and final biomass for different irrigation scenarios (RMSEn < 20%, R² > 0.8 and d > 0.8). According to the results, irrigation depth and interval were important factors affecting yield and biomass. In general, model error increased as the amount of water applied decreased. The least amount of water applied (40 mm) resulted in yield reductions by 76%, 70% and 67% of the greatest amount of water applied (480 mm) for seed yield, pod yield and final biomass, respectively. For each irrigation interval, larger irrigation depth led to lower water productivity (WP) of irrigation (WP_I), but higher WP based on evapotranspiration (WP_ET) and transpiration (WP_T).The average amounts of WP_I, WP_ET, WP_T based on seed yield were 1.2, 0.63 and 1.01 kg m^?3, respectively.  相似文献   

Correlations between circulating insulin-like growth factor-I and thyroxine and cortisol hormone levels,and some biometrical traits in female brood stocks during the late stages of sex maturation and in juvenile Persian sturgeon (<Emphasis Type="Italic">Acipenser persicus</Emphasis>)     

Barzan Bahrami Kamangar  Mohammad Javad Rasaee  Bagher Mojazi Amiri  Behrouz Abtahi  Mahmoud Bahmani 《Fish physiology and biochemistry》2007,33(3):249-257

Relationships between insulin-like growth factor-I (IGF-I) and thyroxine (T4) and cortisol hormones were studied in female brood stocks of Persian sturgeon [Acipenser persicus; caught from both sea water (SW) and fresh water (FW)] during late stages of sex maturation. A number of biometrical traits were also studied that could represent the reproductive and/or growth states of brood stocks, and the possible relationship between IGF-I and growth was assessed in juvenile Persian sturgeons between 1 and 4 years of age. IGF-I, T4 and cortisol were measured in serum samples using commercially available kits. A four-parameter logistic model test was performed between the standard curves and the sample dilutions for each hormone. Parallelism, linearity and regression coefficients for the linearized standard curves and serial dilutions of samples were not significantly different (P < 0.05). Serum IGF-I levels in the juveniles were higher than those in the SW brood stocks, and cortisol levels in the former were lower than those in both brood stocks (P < 0.05). T4 levels in serum samples of juveniles were below the detectable level. IGF-I concentrations in juveniles were correlated with total weight, total length and fork length, but they did not increase significantly with increasing age from 1 to 4 years old (P < 0.05). Compared with SW brood stock, the FW brood stock was younger, had a smaller girth, smaller polarization index and higher ova diameter (P < 0.05). There were no differences between IGF-I and T4 levels in the two brood stocks, but cortisol levels were significantly higher in the FW brood stock. Percentage of fertilization was correlated with serum IGF-I in both brood stocks (P < 0.05). Our results support a role for IGF-I during the juvenile growth and reproductive physiology in female brood stocks of the Persian sturgeon.  相似文献   

Chemical composition and osmolality of seminal fluid of Acipenser persicus; their physiological relationship with sperm motility   总被引：2，自引：0，他引：2  

Sayyed Mohammad Hadi Alavi  Jacky Cosson  Mahmoud Karami  Hossein Abdolhay  & Bagher Mojazi Amiri 《Aquaculture Research》2004,35(13):1238-1243

Determination of semen quality is necessary to understand the basic biochemical processes occurring during motility of sperm and during fertilization to evaluate the reproductive ability of different fish species and to create an optimal environment for storage of spermatozoa; in this regard less information is available for Acipenseridae compared with Cyprinidae and Salmonidae. The aim of the present study is to determine chemical composition and osmolality of seminal fluid and their relationship with sperm motility in Acipenser persicus. The results obtained show that sodium (Na⁺), chloride (Cl^?) and potassium (K⁺) were predominant ions in the seminal plasma and the average of osmolality of seminal plasma was 82.56 mOsm kg^?1. The higher chemical contents and osmolality compared with other sturgeon species reveal species‐specific characteristics and high secretory activity of spermatic duct in A. persicus. Significant positive correlations were observed between osmolality‐Cl^?, Na⁺‐osmolality and Na⁺–Cl^? (P<0.05, P<0.001 and P<0.05 respectively). But statistically significant correlation was not observed between seminal plasma parameters and sperm motility. Probably, the Na⁺ and Cl^? are the main electrolytes playing a major role in maintaining the osmolality of the seminal plasma and the viability of the spermatozoa in vivo.  相似文献   

Sperm density, seminal plasma composition and their physiological relationship in the endangered Caspian brown trout (Salmo trutta caspius)     

Azadeh Hatef  Hamid Niksirat  Bagher Mojazi Amiri  Sayed Mohammad Hadi Alavi  & Mahmoud Karami 《Aquaculture Research》2007,38(11):1175-1181

Sperm density, mineral and organic composition of the seminal plasma and their physiological relationship were investigated in the Caspian brown trout (Salmo trutta caspius). To establish a rapid and accurate method for assessment of sperm density, three different techniques were used: sperm counting, spectrophotometry (at 480 nm) and determination of the spermatocrit. The seminal plasma contained 159.26±8.84 mM sodium (Na), 33.72±2.01 mM potassium (K), 133.04±5.96 mM chlorine (Cl), 1.68±0.2 mM calcium (Ca) and 0.988±0.13 mM magnesium (Mg). The following organic components were found: total protein 0.75±0.14 mg 100 mL⁻¹, cholesterol 2.86±0.58 mg L⁻¹ and glucose 3.81±1.04 mM L⁻¹. The mean sperm density was estimated to be 3.3 × 10⁹ spermatozoa mL⁻¹. The spermatocrit (%) ranged from 25 to 52 in sperm samples. Highly significant linear relationships were found between sperm density and spermatocrit (R²=0.703, P<0.001) and sperm density and optical density (R²=0.909, P<0.001), indicating that optical density can be used as a quick and accurate method of estimating sperm density. Significant relationships were also found between sperm density and Ca, Mg and total protein of seminal plasma. A significant correlation was also observed between the Ca and Mg concentrations (R²=0.774, P<0.01). The following correlations were observed between mineral and organic components: total protein and Ca (R²=0.462, P<0.05), total protein and Mg (R²=0.518, P<0.05) and glucose and Cl (R²=0.374, P<0.05). These parameters should be considered when developing procedures for either artificial fertilization or for cryopreservation of sperm.  相似文献   

In vitro steroidogenesis by testicular fragments and ovarian follicles in a hybrid sturgeon, Bester     

B.M. Amiri  M. Maebayashi  S. Adachi  G.P. Moberg  S.I. Doroshov  K. Yamauchi 《Fish physiology and biochemistry》1999,21(1):1-14

In this study, developmental changes in the steroidogenic capacity of testicular fragments and isolated ovarian follicles of a hybrid sturgeon, Bester, at a variety stage of developments were examined. Testicular fragments or isolated ovarian follicles were incubated in L-15 medium in the presence or absence of different concentrations of five preparations; forskolin, human chorionic gonadotropin (HCG), pregnenolone (P₅), 17-hydroxyprogesterone (17OHP) and testosterone (T) for 18 h at 15 °C. After incubation, concentrations of 11-ketotestosterone (11 KT) (testis) and, 17-estradiol (E₂) (ovarian follicles) and 17,20-dihydroxy-4-pregnen-3-one (DHP) (testis and ovarian follicles) were measured. 11KT was detected in the media following incubation with P₅, 17OHP and T. Its concentration was higher during late spermatogenesis and prespermiation and lower at the degeneration stage. Both P₅ and 17OHP were converted to DHP during the prespermiation stage. Forskolin had little stimulatory effect on the synthesis of 11KT and DHP and HCG did not induce the production of these steroids.E₂ was detected in the medium following incubation of follicles with P₅, 17OHP and T at all stages of oocyte development. The concentration of E₂ in the medium increased during vitellogenesis with the peak production occurring at the tertiary yolk stage. In contrast, the potencies of follicles to produce steroids shifted to the production of DHP during migratory nucleus stage. Forskolin and HCG had little effect on the synthesis of E₂ and DHP. These results demonstrated that the failure of spontaneous spermiation or ovulation is not due to the insufficient synthesis of DHP, but may due to the lack of availability of precursors.  相似文献