Prevalence and antimicrobial resistance of Arcobacter species isolated from poultry meat in Iran     

Ebrahim Rahimi 《British poultry science》2014,55(2):174-180

1. The objective of this study was to determine the prevalence and antimicrobial resistance of Arcobacter spp. isolated from different species of retail poultry meat in Iran.

2. From August 2012 to April 2013, a total of 540 raw poultry meat samples from chicken (n = 100), turkey (n = 100), quail (n = 100), partridge (n = 80), duck (n = 50), ostrich (n = 60) and geese (n = 50) were purchased from randomly selected retail outlets in Shahrekord, Isfahan, Sari and Rasht, Iran.

3. Using culture techniques, 71 of 540 poultry meat samples (13.1%) were positive for Arcobacter spp. The highest prevalence of Arcobacter spp. was found in chicken meat (28.0%), followed by quail (12.0%), duck (11.4%), turkey (11.0%), geese (8.0%), partridge (7.5%) and ostrich (3.3%) meat. The number of A. butzleri isolated from poultry meat samples (90.1%) was significantly higher than A. cryaerophilus (7.1%) and A. skirrowii (2.8%). Significantly more poultry meat samples were found to contain Arcobacter spp. by the PCR assay than by the culture method.

4. Susceptibilities of Arcobacter isolates were determined for 14 antimicrobial drugs using the disk diffusion method. All of the 71 Arcobacter isolates tested were resistant to one or more antimicrobial agents. Resistance to cephalothin and vancomycin (95.8%) was the most common finding, followed by resistance to methicillin, azithromycin and ampicillin. All Arcobacter isolates were susceptible to gentamicin, streptomycin, tetracyclin and kanamycin.

5. The results of this study indicated the importance of poultry meat, especially chicken meat, as potential sources of Arcobacter spp. infection in people. Furthermore, the strains indicated resistance to a broad spectrum of antibiotics.  相似文献   

Survey of microbial quality of drinking water in rural areas of Kashan-Iran in second half of 2008     

Miranzadeh MB  Heidari M  Mesdaghinia AR  Younesian M 《Pakistan journal of biological sciences: PJBS》2011,14(1):59-63

The objective of the study is surveying microbial quality of drinking water in Kashan rural areas and determining the rural population that using safe water in terms of microbial quality in second-half of 2008. In this cross-sectional study, microbial quality of water in all rural areas was determined in 3 stages based on 3 parameters as Total Coliforms, Fecal Coliforms and Heterotrophic Plate Count (HPC). The results of this study illustrates that 100.0, 47.71 and 92.99% population in under coverage and non under coverage areas of Kashan Rural Water and Wastewater Company (KRWWC) and all Kashan rural areas, respectively using safe water in terms of Fecal Coliforms and 98.4, 21.2 and 88.00% population in under coverage and non under coverage areas of KRWWC rural areas and all Kashan rural areas, respectively using safe water in terms of Total Coliforms. There is also a meaningful difference in microbial quality between under coverage and non-under coverage rural areas. The results of this study express that the fecal contamination in under coverage rural areas is excellent, but there is a bad condition in non-under coverage areas. Generally, the microbial quality in all Kashan rural areas is approximately equal to national microbial criteria. Its been also illustrated that the role of KRWWC in supplying safe drinking water in terms of microbial quality for rural population is very important.  相似文献   

Evaluation of Salinity Effects on Ionic Balance and Compatible Solute Contents in Nine Grape (Vitis L.) Genotypes     

Nayer Mohammadkhani  Reza Heidari  Nasser Abbaspour  Fatemeh Rahmani 《Journal of plant nutrition》2014,37(11):1817-1836

The salinity tolerance of nine grape genotypes was studied. Salinity was applied as nutrient solutions containing 0, 25, 50, and 100 mM sodium chloride (NaCl) for two weeks. Growth was significantly reduced by salinity, whereas chloride (Cl^?) and sodium (Na⁺) contents increased. Sodium ion accumulation exceeded that of Cl^? in all treatments. Shirazi and H₆ had higher and lower Cl^? concentrations in their lamina than others. There were significant positive correlations (P < 0.01) between Cl^? and Na⁺ and negative correlation between Na⁺ and potassium (K⁺) in roots and laminas of all genotypes. Soluble sugars, proline, and glycine betaine contents increased in laminas of all of the genotypes with moderate salinity. There were positive correlations (P < 0.01) between lamina and root Na⁺ and Cl^? contents and compatible solutes in all genotypes. Overall results revealed that unlike Shirazi with higher Na⁺ and Cl^? accumulation in shoot, H₆ showed a higher capacity to restrict Na⁺ and Cl^? transport to shoot.  相似文献   

Über die Schäden vonNycteola asiatica Krul. (Lepid., Noctuidae) an Pappeln im Iran     

Ebrahim Adeli 《Journal of pest science》1971,44(8):119-120

Ohne Zusammenfassung  相似文献   

Essential oil variation among and within six Achillea species transferred from different ecological regions in Iran to the field conditions     

Mehdi Rahimmalek  Badraldin Ebrahim Sayed Tabatabaei  Nematolah Etemadi  Sayed Amir Hossein Goli  Ahmad Arzani  Hossein Zeinali 《Industrial Crops and Products》2009,29(2-3):348-355

The compositions of essential oils of 19 accessions belonging to six different Achillea species, transferred from the natural habitats in 10 provinces of Iran to the field conditions, were assessed. The relationship between the leaf areas of selected accessions with their essential oil content was also investigated. Essential oil yield of dried plants obtained by hydro-distillation ranged from 0.1 to 2.7% in leaves. Results indicated a significant variation in oil composition among and within species. Total of 94 compounds were identified in 19 accessions belonging to the six species of A. millefolium, A. filipendulina, A. tenuifolia, A. santolina, A. biebersteinii and A. eriophora. The major constituents of the leaves in the tested genotypes were determined as germacrene-D, bicyclogermacrene, camphor, borneol, 1,8-cineole, spathulenol and bornyl acetate. According to the major compounds, four chemotypes were defined as: (I) spathulenol (1.64–34.31%) + camphor (0.2–15.61%) (7 accessions); (II₁) germacrene-D (18.78–23.93%) + borneol (7.93–8.26%) + bornyl acetate (11.56–14.66%) (5 accessions); (II₂) germacrene-D (13.28–36.28%) + bicyclogermacrene (5.93–8.4%) + 1,8-cineole (15.26–19.41%) + camphor (14.95–23.32%) (2 accessions); (III) borneol + camphor (52.04–63.27) (2 accessions); (IV) germacrene-D (45.86–69.64%) (3 accessions). The relationships of chemotypes with soil type and climatic conditions of collected regions were assessed, as probable reasons of high variations in essential oil components, and discussed.  相似文献   

Induction of transgenic hairy roots in Trigonella foenum-graceum co-cultivated with Agrobacterium rhizogenes harboring a GFP gene     

Zeynab Shahabzadeh  Bahram Heidari  Reza Faramarzi Hafez 《Journal of Crop Science and Biotechnology》2013,16(4):263-268

An important feature of A. rhizogenes-induced hairy roots is their unique ability for investigation of gene function and production of secondary metabolites such as diosgenin in fenugreek. In order to evaluate the transformation frequency and the efficiency of transgenic hairy root induction, leaf and stem explants from two fenugreek ecotypes, Karaj and Bushehr, were infected with three concentrations of OD₆₀₀= 0.8, 1.2, and 1.6 of A. rhizogenes strain K599 harboring a GFP gene. Regardless of ecotype, the ability of stem explants for the induction of hairy roots (8.09) and the transformation frequency (81.3%) was higher compared with leaf explants with the values of 5.97 and 71.88%, respectively. The number of transgenic GFP-positive hairy roots ranged from 4.2 to 13.5 in the Karaj ecotype and 3.8 to 9.9 in Bushehr. The highest transgenic hairy root (8.76), the transformation frequency (79.76%), and the growth rate of transgenic roots (0.77 d^?1) were obtained from infection with K599 at OD₆₀₀= 1.2, while the lowest belonged to the bacterial concentration of OD₆₀₀=1.6. Although the ecotype Bushehr had lower total roots (7.53) and transgenic hairy roots (6.08), it showed higher transformation frequency (79.56%) than Karaj (73.63%). Therefore, the results indicate the importance of genotype, type of explant and bacterial concentration in breeding for induction of transgenic hairy roots and consequently, production of secondary metabolites in fenugreek.  相似文献   

Bioactivity of aqueous and organic extracts of sea cucumber Holothuria leucospilota (Brandt 1835) on pathogenic Candida and Streptococci     

Ghadiri  Motahereh  Kazemi  Soodabeh  Heidari  Behrooz  Rassa  Mehdi 《International Aquatic Research》2018,10(1):31-43

International Aquatic Research - Holothurians (sea cucumbers) exist almost in all benthic marine environments and they are an important species for the commercial fisheries. In this study, the...  相似文献   

Sustainability assessment of potato fields using the DEXi decision support system in Hamadan Province,Iran     

Mohammed Ebrahim Rezaei  Morteza Barmaki  Hadi Veisi 《农业科学学报》2018,17(11):2583-2595

Potato is a staple food crop and the most important agricultural commodity, which critically affects food security and economic stability in Hamadan Province, Iran. Ex-ante sustainability appraisal of new cropping systems and their comparisons with conventional systems can increase the efficiencies of innovations and changes within the production process. This study aimed to explore the sustainability levels of potato cropping systems in Hamadan Province, Iran. To this end, DEXi methodology was developed for the sustainability assessment of the cropping systems. For assessing the three dimensions of environmental, economic, and social sustainability in the four systems of traditional potato system (TPS), Quasi-industrial potato system (QIPS), industrial potato system (IPS), and government-promoted potato system (GPPS), five groups of features were utilized: (1) irrigation method; (2) seed placement; (3) farm machinery use; (4) agrochemical use; and (5) rotation. The impact assessments of the cropping systems were based on two, three, and two groups of ecological, social, and economic indicators, respectively. Employment, supply chain, protection, operational difficulty, productivity, profitability, input use, and biodiversity resulted in 21 basic indicators and 13 aggregated indicators, by which the cropping systems were described. The results revealed that GPPS with distinctive economic and social profiles could have a better overall sustainability despite the fact that some indicators like biodiversity could be negatively affected. Finally, three strategies were recommended for the sustainability of GPPS as follows: biodiversity enhancement, input substitution, and integrated water management.  相似文献   

Characterization of a unique copper resistance gene cluster in <Emphasis Type="Italic">Xanthomonas campestris</Emphasis> pv. <Emphasis Type="Italic">campestris</Emphasis> isolated in Trinidad,West Indies     

Franklin Behlau  Alberto M. Gochez  Alshia Janel Lugo  Winston Elibox  Gerald V. Minsavage  Neha Potnis  Frank F. White  Mohamed Ebrahim  Jeffrey B. Jones  Adesh Ramsubhag 《European journal of plant pathology / European Foundation for Plant Pathology》2017,147(3):671-681

Whole genome sequencing of a copper resistant (Cu^R) black rot strain of Xanthomonas campestris pv. campestris (Xcc) isolated from a broccoli plant in Trinidad revealed a unique operon for copper resistance. The cop genes of strain Xcc-BrA1 were determined to be present on a 160 to 180 kb plasmid shown to be non-conjugative with other xanthomonads. While nucleotide comparison of a putative 8.0 Kbp copLABMGF gene cluster identified in Xcc-BrA1 genome did not reveal any homologous region with other known Cu^R Xanthomonas strains from diverse origins, the comparison of the translated amino acid sequence indicated similarity with X. citri, X. c. pv. citrumelonis and X. vesicatoria Cop proteins. Cloning of the copLAB gene cluster from Xcc-BrA1 conferred copper resistance to other copper-sensitive xanthomonads. Although Xcc-BrA1 harbors copLAB genes with similar sizes and organization and is able to grow on Cu-amended medium as other Cu^R xanthomonads, the phylogenetic analysis of nucleotide sequences indicates that the cop cluster in Xcc-BrA1 is unique and distantly related to other copLAB genes from Xanthomonas and Stenotrophomonas. The origin of copper resistance genes in Xcc-BrA1 is likely a result of horizontal gene acquisition from a still unknown phylloplane cohabitant. The findings of this study have implications for the management of crop diseases caused by Cu^R xanthomonads. Future studies could focus on and determining the distribution, overall importance and appropriate control measures for strains harbouring these unique genes.  相似文献   

Molecular identification of 16SrII-D subgroup phytoplasmas associated with chickpea and faba bean in Sudan     

Ana Alfaro-Fernández  Mai Abdalla Ali  Fadia Mobarak Abdelraheem  Ebrahim Abd Elhameed Saeed  María Isabel Font San Ambrosio 《European journal of plant pathology / European Foundation for Plant Pathology》2012,133(4):791-795

In January 2011, symptomatic chickpea and faba bean plants were observed in fields located in the Gezira state (Sudan). Faba bean plants showed yellowing and stunting, whereas chickpea plants presented yellowing, reddening and little leaves. The disease etiology was investigated using nested polymerase chain reaction (PCR) with phytoplasma-specific primers which amplify a fragment of the 16S rRNA gene. Sequencing and restriction fragment length polymorphism (RFLP) analyses revealed that the tested phytoplasmas belonged to the group 16SrII. Phylogenetic analyses of the 16S rRNA gene of the obtained sequences indicated that the chickpea and faba bean phytoplasmas from Sudan were more closely related to the phytoplasmas subgroup 16SrII-D. To our knowledge, this is the first report of phytoplasmas from the group 16SrII-D infecting chickpea in Sudan, and faba bean worldwide.  相似文献