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11.
Uriarte A Moissonnier P Thibaud JL Reyes-Gomez E Devauchelle P Blot S 《The Canadian veterinary journal. La revue veterinaire canadienne》2011,52(7):748-752
The cases of 7 adult dogs with generalized seizures managed by surgical excision and radiation therapy for frontal lobe meningiomas were reviewed. The neurological examination was unremarkable in 6 of the 7 dogs. Five dogs were operated on using a bilateral transfrontal sinus approach and 2 using a unilateral sinotemporal approach to the frontal lobe. One dog was euthanized 14 d after surgery; radiation therapy was initiated 3 wk after surgery in the remaining 6 dogs. Long-term follow-up consisted of neurological examination and magnetic resonance imaging (MRI) and/or computed tomography (CT) scan after radiation therapy. The mean survival time for dogs that had surgery and radiation therapy was 18 mo after surgery. Frontal lobe meningiomas have been associated with poor prognosis. However, the surgical approaches used in these cases, combined with radiation therapy, allow a survival rate for frontal lobe meningiomas similar to that for meningiomas located over the cerebral convexities. 相似文献
12.
Delphine Ansan-Melayah Thierry Rouxel Jean Bertrandy Bruno Letarnec Edouard Mendes-Pereira Marie-Hélène Balesdent 《European journal of plant pathology / European Foundation for Plant Pathology》1997,103(9):835-841
Field experiments were conducted in Versailles, France, to assess blackleg resistance of Brassica napus cultivars Quinta and Glacier under natural infection conditions. Blackleg disease severity was assessed twice during growth of B. napus. Quinta resistance was highly expressed as only 13% to 18% of the plants exhibited leaf symptoms in December, whereas Glacier and other cultivars displayed more than 80% of infected plants. In June (harvest), 70% (first year) to 41.5% (second year) of Quinta plants were canker-free. In contrast, Glacier was as infected as the susceptible control cultivars, with more than 88% of plants displaying canker. The Leptosphaeria maculans population structure was examined in parallel. Based on soluble protein patterns, 9% of the 299 fungal isolates collected were characterized as Tox0 species, and belonged to the NA1 sub-group. All but two of Tox0 isolates were isolated from atypical dark necrotic leaf lesions, mainly occurring on Quinta. In contrast, the Tox+ isolates were recovered from typical leaf lesions. Following a cotyledon inoculation test on the differential set Westar, Quinta and Glacier, 92 to 95% of Tox+ isolates collected on susceptible cultivars were characterized as PG3 isolates, i.e. avirulent on Quinta. The remaining Tox+ isolates belong to PG4, i.e. virulent on the three cultivars. No PG2 isolate, i.e. avirulent on both Quinta and Glacier, was identified in the sampling. The present study suggests that specific resistance expressed at the cotyledon level can be efficient under field conditions where the corresponding avirulent races of the pathogen are prevalent. 相似文献
13.
Zon Aboubacar Oumar Kouassi Edouard Konan Ouédraogo Amadé 《Genetic Resources and Crop Evolution》2021,68(5):1731-1745
Genetic Resources and Crop Evolution - Palms constitute vital species for local people’s well-being, especially in West Africa. This analytic review aims at providing an overview of West... 相似文献
14.
In criminal investigations, information on the origin of soils may be crucial for solving cases. The biological complexity of soil may potentially be used for sorting and differentiating between soil samples. Nucleic-acid based analyses of soil microbial populations are powerful tools, routinely used in studies of this habitat. Application of such approaches in forensics implies that a standardized DNA extraction method has to be applied to all samples. In this study, several DNA extraction protocols were compared. An improvement on the method proposed by Tsai and Olson (1991) was found to be most suited to extract DNA from various soil types, including from small samples. A blind test on soils from a crime, an alibi scene and unrelated locations was conducted to evaluate the potential of environmental PCR and denaturating gradient gel electrophoresis for use in forensic science. In most cases, soil patterns clustered according to soil type and location. 相似文献
15.
Yuliaxis Ramayo-Caldas Laura Zingaretti Milka Popova Jordi Estellé Aurelien Bernard Nicolas Pons Pau Bellot Núria Mach Andrea Rau Hugo Roume Miguel Perez-Enciso Philippe Faverdin Nadège Edouard Dusko Ehrlich Diego P. Morgavi Gilles Renand 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2020,137(1):49-59
Mitigation of greenhouse gas emissions is relevant for reducing the environmental impact of ruminant production. In this study, the rumen microbiome from Holstein cows was characterized through a combination of 16S rRNA gene and shotgun metagenomic sequencing. Methane production (CH4) and dry matter intake (DMI) were individually measured over 4–6 weeks to calculate the CH4 yield (CH4y = CH4/DMI) per cow. We implemented a combination of clustering, multivariate and mixed model analyses to identify a set of operational taxonomic unit (OTU) jointly associated with CH4y and the structure of ruminal microbial communities. Three ruminotype clusters (R1, R2 and R3) were identified, and R2 was associated with higher CH4y. The taxonomic composition on R2 had lower abundance of Succinivibrionaceae and Methanosphaera, and higher abundance of Ruminococcaceae, Christensenellaceae and Lachnospiraceae. Metagenomic data confirmed the lower abundance of Succinivibrionaceae and Methanosphaera in R2 and identified genera (Fibrobacter and unclassified Bacteroidales) not highlighted by metataxonomic analysis. In addition, the functional metagenomic analysis revealed that samples classified in cluster R2 were overrepresented by genes coding for KEGG modules associated with methanogenesis, including a significant relative abundance of the methyl-coenzyme M reductase enzyme. Based on the cluster assignment, we applied a sparse partial least-squares discriminant analysis at the taxonomic and functional levels. In addition, we implemented a sPLS regression model using the phenotypic variation of CH4y. By combining these two approaches, we identified 86 discriminant bacterial OTUs, notably including families linked to CH4 emission such as Succinivibrionaceae, Ruminococcaceae, Christensenellaceae, Lachnospiraceae and Rikenellaceae. These selected OTUs explained 24% of the CH4y phenotypic variance, whereas the host genome contribution was ~14%. In summary, we identified rumen microbial biomarkers associated with the methane production of dairy cows; these biomarkers could be used for targeted methane-reduction selection programmes in the dairy cattle industry provided they are heritable. 相似文献
16.
Pillai RS Bhattacharyya SN Artus CG Zoller T Cougot N Basyuk E Bertrand E Filipowicz W 《Science (New York, N.Y.)》2005,309(5740):1573-1576
MicroRNAs (miRNAs) are approximately 21-nucleotide-long RNA molecules regulating gene expression in multicellular eukaryotes. In metazoa, miRNAs act by imperfectly base-pairing with the 3' untranslated region of target messenger RNAs (mRNAs) and repressing protein accumulation by an unknown mechanism. We demonstrate that endogenous let-7 microribonucleoproteins (miRNPs) or the tethering of Argonaute (Ago) proteins to reporter mRNAs in human cells inhibit translation initiation. M(7)G-cap-independent translation is not subject to repression, suggesting that miRNPs interfere with recognition of the cap. Repressed mRNAs, Ago proteins, and miRNAs were all found to accumulate in processing bodies. We propose that localization of mRNAs to these structures is a consequence of translational repression. 相似文献
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18.
Lisa Shindo Christelle Belingard Jean-Louis Edouard Mélanie Saulnier 《Annals of Forest Science》2017,74(4):67
? Key message This paper presents ring width data of silver fir trees (Abies alba Mill.) from buildings and living trees from 1214 to 2009 in southeastern France. A 796-year chronology spanning the period 1214–2009 has been built. Data can be used for dating projects, paleoecology studies, and climate reconstructions. Dataset access is at https://hal.archives-ouvertes.fr/hal-01528324 . Associated metadata is available at https://metadata-afs.nancy.inra.fr/geonetwork/srv/fre/catalog.search#/metadata/a74b4869-318c-458c-a5fe-f9ff44a5082c 相似文献
19.
Ahmed Maouche Edouard Curran Anne-Sophie Goupil Elisabeth Sambroni Johanna Bellaiche Florence Le Gac Jean-Jacques Lareyre 《Fish physiology and biochemistry》2018,44(6):1599-1616
The present study aimed to investigate whether the Gfra1/Gdnf and/or Kit/Kitlg regulatory pathways could be involved in the regulation of spermatogonial cell proliferation and/or differentiation in fish. Homologs of the mammalian gfra1, gdnf, kitr, and kitlg genes were identified in gnathostomes and reliable orthologous relationships were established using phylogenetic reconstructions and analyses of syntenic chromosomal fragments. Gene duplications and losses occurred specifically in teleost fish and members of the Salmoninae family including rainbow trout (Oncorhynchus mykiss) and Atlantic salmon (Salmo salar). Some duplicated genes exhibited distinct spatiotemporal expression profiles and were differently regulated by hormones in rainbow trout. Undifferentiated type A spermatogonia expressed higher levels of kitrb and kitra2 making them possible target cells for the gonadal kitlgb and somatic kitlga before the onset of spermatogenesis. Interestingly, gdnfa and gdnfb ohnologous genes were poorly expressed before the onset of spermatogenesis. The expression level of gdnfb was correlated with that of the vasa gene suggesting that the late increased abundance of gdnfb during spermatogenesis onset was due to the increased number of spermatogonial cells. gfra1a2 was expressed in undifferentiated type A spermatogonia whereas gfra1a1 was mainly detected in somatic cells. These observations indicate that the germinal gdnfb ligand could exert autocrine and paracrine functions on spermatogonia and on testicular somatic cells, respectively. Fsh and androgens inhibited gfra1a2 and gdnfb whereas gfra1a1 was stimulated by Fsh, androgens, and 17α, 20β progesterone. Finally, our data provide evidences that the molecular identity of the male germ stem cells changes during ontogenesis prior to spermatogenesis onset. 相似文献
20.
Evelyne Picard‐Meyer Veronique Beven Edouard Hirchaud Cdric Guillaume Grald Larcher Emmanuelle Robardet Alexandre Servat Yannick Blanchard Florence Cliquet 《Zoonoses and public health》2019,66(2):254-258
Bat rabies cases are attributed in Europe to five different Lyssavirus species of 16 recognized Lyssavirus species causing rabies. One of the most genetically divergent Lyssavirus spp. has been detected in a dead Miniopterus schreibersii bat in France. Brain samples were found positive for the presence of antigen, infectious virus and viral RNA by classical virological methods and molecular methods respectively. The complete genome sequence was determined by next‐generation sequencing. The analysis of the complete genome sequence confirmed the presence of Lleida bat lyssavirus (LLEBV) in bats in France with 99.7% of nucleotide identity with the Spanish LLEBV strain (KY006983). 相似文献