Pyometra and unilateral uterine horn torsion in a sheep          下载免费PDF全文

JM Castillo  JC Dockweiler  SH Cheong  M Diel de Amorim 《Reproduction in domestic animals》2018,53(1):274-277

A 13‐year‐old Romanov sheep presented for evaluation of vaginal discharge, depression and anorexia. Blood work demonstrated mature neutrophilia and marked hyperlactatemia. Transrectal and transabdominal ultrasound revealed echogenic fluid (pus) within the uterus. Purulent uterine fluid culture yield Escherichia coli. A diagnosis of pyometra was made. After medical treatment without clinical improvement, an exploratory laparotomy was performed, and a 360‐degree uterine left horn torsion was identified with the pyometra and multiple corpora lutea on both ovaries. Ovariohysterectomy was performed and the ewe recovered uneventfully.  相似文献   

Does the level of forage neutral detergent fiber affect the ruminal fermentation,digestibility and feeding behavior of goats fed cactus pear?          下载免费PDF全文

Ricardo Martins Araujo Pinho  Edson Mauro Santos  Juliana Silva de Oliveira  Gleidson Giordano Pinto de Carvalho  Thiago Carvalho da Silva  Alberto Jefferson da Silva Macêdo  Yohana Rosaly Corrêa  Anderson de Moura Zanine 《Animal Science Journal》2018,89(10):1424-1431

This study aimed to evaluate the effects of forage neutral detergent fiber (fNDF) levels on the voluntary feed intake, digestibility, ruminal fermentation and feeding behavior of goats fed diets with cactus pear. Five non‐lactating ruminally cannulated goats fed ad libitum were randomly assigned to a 5 × 5 Latin square design. Treatments consisted of levels of fNDF at 0, 109, 222, 339 and 463 g/kg of dry matter (DM) in cactus pear‐based diets. The intakes of DM and NDF were quadratically affected (p ≦ .045) by fNDF levels. Voluntary water intake (VWI) increased linearly as the fNDF levels increased in the diet. The digestibility coefficients of organic matter, NDF and ether extract and total digestible nutrients concentration were quadratically affected (p ≦ .048) by fNDF levels. The ruminal pH linearly increased (p = .001) with fNDF levels, ranging from 5.44 to 5.81 for diets containing 0 and 463 g fNDF/kg DM, respectively. The fNDF levels promoted a linear increase (p = .006) in chewing time, linearly decreased (p = .007) resting time and quadratically affected (p = .033) rumination time. The inclusion of fNDF in the diets provided favorable conditions for ruminal function, digestibility and feeding behavior in goats fed diets containing cactus pear.  相似文献   

Performance of dual and triple fungicide premixes for managing soybean rust across years and regions in Brazil: A meta-analysis     

Jhonatan P. Barro  Kaique S. Alves  Cláudia V. Godoy  Alfredo R. Dias  Carlos A. Forcelini  Carlos M. Utiamada  Edson R. de Andrade Júnior  Fernando C. Juliatti  Fernando J. Grigolli  Heraldo R. Feksa  Hercules D. Campos  Ione C. P. V. Chaves  Ivan P. Araújo Júnior  João Mauricio T. Roy  José Nunes Júnior  Luana M. R. Belufi  Luciana C. Carneiro  Luís H. C. P. Silva  Marcelo G. Canteri  Marcio M. Goussain Júnior  Marina Senger  Maurício C. Meyer  Moab D. Dias  Mônica A. Müller  Mônica C. Martins  Mônica P. Debortoli  Nédio R. Tormen  Silvânia H. Furlan  Tiago F. Konageski  Valtemir J. Carlin  Wilson S. Venâncio  Emerson M. Del Ponte 《Plant pathology》2021,70(8):1920-1935

Soybean rust in Brazil is currently controlled with several commercial fungicide premixes composed of demethylation inhibitors (EPOXiconazole, CYPRoconazole, PROThioconazole, TEBUconazole), quinone-outside inhibitors (AZOXystrobin, TriFLoXystrobin, PYRAclostrobin, PICOxystrobin), and succinate demethylation inhibitors (BENZovindiflupyr, BIXaFen, FLUXapyroxad). Here, we summarize the performance of eight premixes evaluated in 177 cooperative trials conducted in 46 locations across 10 states from 2015 to 2020. All fungicide treatments were sprayed three times starting at R1/R2. Percentage control (, %), from back-transforming meta-analytic estimates of the log of the ratio, ranged from 56.2% (PICO + CYPR) to 76.8% (BIXF + TFLX + PROT). Estimates of mean yield difference (, kg/ha) between fungicide-treated and untreated plots were greatest for BIXF + TFLX + PROT (1,080) followed by PICO + BENZ (1,010), PYRA + EPOX + FLUX (981.5), AZOX + BENZ (910), TFLX + PROT (891), PICO +TEBU (682), TFLX + CYPR (646), and PICO + CYPR (600). Significant declines in both and in as little as 4 years were detected for AZOX + BENZ (35.3%; 550 kg/ha) and PICO + BENZ (15.5%; 359.8 kg/ha). Variance in was reduced by the inclusion of baseline severity as covariate. In trials where baseline disease was ≥70%, yield was 250 kg/ha greater compared to areas with low baseline disease. Disease control and yield response were generally greater in the south-east, where the frequency of profitable scenarios was 30% higher on average than in the north-west. Results of this meta-analysis are critical for supporting decisions during planning of fungicide programmes.  相似文献   

Electrospray ionization mass spectrometry fingerprinting of Brazilian artisan cachaça aged in different wood casks     

de Souza PP  Siebald HG  Augusti DV  Neto WB  Amorim VM  Catharino RR  Eberlin MN  Augusti R 《Journal of agricultural and food chemistry》2007,55(6):2094-2102

We have investigated the capability of direct infusion electrospray ionization mass spectrometry in the negative ion mode, ESI(-)-MS, to differentiate representative samples of artisan cacha?a, a Brazilian sugar cane distillate of large production, aged in four different types of wood casks: amburana (Amburana cearensis), jequitibA (Cariniana estrellensis), balm (Myroxylon peruiferum), and oak (Quercus rubra). The ESI(-)-MS were found to be very characteristic, showing sets of diagnostic ions for each of the four types of samples: amburana (m/z 271, 313, 377), jequitibA (m/z 143, 171, 255), balm (m/z 137, 269, 283, 297), and oak (m/z 197, 301, 307). Furthermore, principal component (PCA) and hierarchical cluster analysis (HCA), applied to the ESI(-)-MS data, divided these samples into four definite categories. The influence of the aging time on the ESI(-)-MS fingerprints of the cacha?a samples stored in oak casks was also established. An inversion in the relative intensity of the diagnostic ions of m/z 307 and 301 is detected in the ESI(-)-MS as the aging time increased from 1 to 2 years. The chemical structures of the major cacha?a components were proposed on the basis of the following: (a) the comparison of the ESI(-)-MS/MS of the diagnostic anions with those of the authentic anions or (b) the interpretation of the fragmentation patterns of the previously unknown diagnostic anions. Hence, direct infusion ESI(-)-MS allows not only a rapid, simple, and accurate way to distinguish among cacha?a samples stored in different wood casks but also monitoring changes in their chemical composition according to the aging time.  相似文献   

Inhibition of photophosphorylation and electron transport chain in thylakoids by lasiodiplodin, a natural product from Botryosphaeria rhodina     

Veiga TA  Silva SC  Francisco AC  Filho ER  Vieira PC  Fernandes JB  Silva MF  Müller MW  Lotina-Hennsen B 《Journal of agricultural and food chemistry》2007,55(10):4217-4221

Four natural products were isolated from the fungus Botryosphaeria rhodina, and their effects on photosynthesis were tested. Only lasiodiplodin (1) inhibited ATP synthesis and electron flow from water to methylviologen; therefore, it acts as a Hill reaction inhibitor in freshly lysed spinach thylakoids. Photosystem I and II and partial reactions as well as ATPase were measured in the presence of 1. Three new different sites of 1 interaction and inhibition were found: one at CF1, the second in the water-splitting enzyme, and the third at the electron-transfer path between P680 and QA; these targets are different from that of the synthetic herbicides present. Electron transport chain inhibition by 1 was corroborated by fluorescence induction kinetics studies.  相似文献   

Quercetin increases oxidative stress resistance and longevity in Saccharomyces cerevisiae     

Belinha I  Amorim MA  Rodrigues P  de Freitas V  Moradas-Ferreira P  Mateus N  Costa V 《Journal of agricultural and food chemistry》2007,55(6):2446-2451

Quercetin, the major flavonol found in several fruits and vegetables, is a natural antioxidant with potential anticancer and antiaging activities. In this paper, the effect of quercetin in Sacharomyces cerevisiae cells submitted to oxidative stress was studied. Hydrogen peroxide resistance increased in cells pretreated with quercetin. Cellular protection was correlated with a decrease in oxidative stress markers, namely, levels of reactive oxygen species, glutathione oxidation, protein carbonylation, and lipid peroxidation. The acquisition of H2O2 resistance was not associated with the induction of antioxidant defenses or with iron chelation. Oxidative stress is a limiting factor for longevity. In agreement, quercetin also increased 60% chronological life span. These results support the utilization of yeast as a useful model to screen in vivo for natural antioxidants with putative health beneficial effects.  相似文献   

Genetic variability of banana with ornamental potential     

Everton Hilo de Souza  Maria Angélica Pereira de Carvalho Costa  Fernanda Vidigal Duarte Souza  Davi Silva Costa Junior  Edson Perito Amorim  Sebastião de Oliveira e Silva  Janay Almeida dos Santos-Serejo 《Euphytica》2012,184(3):355-367

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Comparison of two immunochromatographic assays and the indirect immunofluorscence antibody test for diagnosis of Trypanosoma cruzi infection in dogs in south central Louisiana     

Prixia D. Nieto  Roger Boughton  Patricia L. Dorn  Frank Steurer  Syamal Raychaudhuri  Javan Esfandiari  Edson Gonalves  James Diaz  John B. Malone 《Veterinary parasitology》2009,165(3-4):241-247

Two rapid tests evaluated in dogs considered to be of high risk of infection with the Chagas parasite Trypanosoma cruzi using two immunochromatographic assays: Trypanosoma Detect™ for canine, InBios, Seattle, WA and CHAGAS STAT-PAK™ assay, Chembio Diagnostic Systems, Medford, NY, in south central Louisiana. For this purpose a serological survey was carried out in a total of 122 dogs and a serum bank was created. These 122 animals were first tested by IFAT that was used as the standard test. From the serum bank 50 samples were tested using the two rapid Chagas assays and results compared to the standard test IFAT. The serological survey using IFAT showed a prevalence of T. cruzi infection in 22.1% of the tested dogs. In the immunochromatographic assays, 13 and 11 animals were positive on rapid assay: Trypanosoma Detect™ for canine, InBios and CHAGAS STAT-PAK™, Chembio Diagnostic Systems, respectively compared to 11 positive by IFAT. These two immunochromatographic tests have shown high susceptibility and specificity compared to our standard method IFAT. The rapid, easy and accurate screening assays used in conjunction with confirmatory tests, would be an excellent tool for veterinarians to diagnose T. cruzi infection. Early detection of T. cruzi infection may prevent complications through an effective treatment. Greater awareness by veterinarians of the risk, clinical findings, history along with diagnostic methods will contribute greatly to an understanding of the true prevalence of Chagas disease in dogs in Louisiana.  相似文献   

Histopathology of black spot symptoms in sweet orange<Emphasis Type="Italic">s</Emphasis>     

João Paulo Rodrigues Marques  Marcel Bellato Spósito  Alexandre Furtado Silveira Mello  Lilian Amorim  Matheus Mondin  Beatriz Appezzato-da-Glória 《European journal of plant pathology / European Foundation for Plant Pathology》2012,133(2):439-448

In Brazil, citrus black spot (CBS) caused by Guignardia citricarpa is a major disease that has different symptoms on fruit. In this study, fruit of Citrus sinensis infected by G. citricarpa and showing the symptoms false melanosis, freckle spot and hard spot were cross-sectioned and analysed anatomically and histochemically by light microscopy. Immuno-histological assays were performed. All symptoms were accompanied by a thickening of the cuticle. False melanosis lesions did not contain pycnidia and remained restricted to the epicarp or to the first layers of the mesocarp. The stomata in this type of lesion showed phenolic compounds in the guard cells and in the sub-stomatal chamber. In some samples, the guard cells and their surrounding cells lysed, and a wound meristem began to form underneath them. Freckle spot and hard spot lesions had very similar histological alterations to the epicarp and mesocarp, but in our samples only hard spot lesions contained pycnidia. Both of these symptoms were accompanied by protein inclusions. Epidermal and sub-epidermal cells located in the oil-gland region were obliterated, causing alterations in these structures. All symptoms had regions that stained strongly for lipids and phenols.  相似文献   

Cauliflower stunt associated with a phytoplasma of subgroup 16SrIII-J and the spatial pattern of disease     

M. C. C. Rappussi  B. Eckstein  D. Fl?res  I. C. R. Haas  L. Amorim  I. P. Bedendo 《European journal of plant pathology / European Foundation for Plant Pathology》2012,133(4):829-840

Since 2000, a disease has occurred with high levels of incidence in crops of cauliflower grown in the green belt area of the city of S?o Paulo, Brazil. The symptoms are characterized by stunting, malformation of the inflorescence, reddening leaves, and vascular necrosis, suggesting infection by phytoplasma. These symptoms are similar to those described in Brassicas species affected by the aster yellows (16SrI) group of phytoplasma. In the present study, a phytoplasma from the 16SrIII-J subgroup was identified in cauliflower plants based on actual and virtual RFLP patterns and phylogenetic analysis, and was distinct from the phytoplasmas frequently associated with aster yellows disease in Brassicas. Pathogenicity assays using dodder confirmed that the identified phytoplasma is the agent of the observed disease, which is here designated as cauliflower stunt. Consequently, this species of Brassica may be recognized as a new host for subgroup 16SrIII-J, which has frequently been found in diverse species cultivated in Brazil. The spatial pattern of diseased plants was determined in ten cauliflower plots of 300 to 728 plants each. All plants in these plots were evaluated by visual assessments, assigned as diseased or healthy and mapped. The dispersion index and Taylor’s power law were determined for various quadrat sizes and the results showed that the diseased plants were distributed in a random pattern in fields with a low disease incidence and in an aggregated pattern in fields with a disease incidence greater than 25?%. According to an isopath area analysis, diseased plants were predominantly present in the field borders, suggesting that the pathogen is possibly introduced by vector(s) from the external area.  相似文献