Prior and concomitant dehydroepiandrosterone treatment affects immunologic response of cultured macrophages infected with Trypanosoma cruzi in vitro?     

Kuehn CC  Oliveira LG  Santos CD  Augusto MB  Toldo MP  do Prado JC 《Veterinary parasitology》2011,177(3-4):242-246

DHEA, a steroid hormone synthesized from cholesterol by cells of the adrenal cortex, plays an essential role in enhancing the host's resistance to different experimental infections. Receptors for this hormone can be found in distinct immune cells (especially macrophages) that are known to be the first line defense against Trypanosoma cruzi infection. These cells operate through an indirect pathway releasing nitric oxide (NO) and cytokines such TNF-α and IL-12 which in turn trigger an enhancement of natural killer cells and lymphocytes which finally secrete pro and anti-inflammatory cytokines. The effects of pre- and post-infection DHEA treatment on production of IL-12, TNFα and NO were evaluated. T. cruzi infected macrophages post treated with DHEA displayed enhanced concentrations of TNF-α, IL-12 and NO. Probably, the mechanisms that induced the production of cytokines by infected cells are more efficient when the immune system has been stimulated first by parasite invasion, suggesting that the protective role of DHEA is greater when administered post infection.  相似文献   

全球禽肉生产和贸易中的沙门菌风险评估与控制   总被引：1，自引：0，他引：1  

G.Mead  A.M.Lammerding  N.Cox  M.P.Doyle  F.Humbert  A.Kulikocskiy  A.Panin  V.P.do Nascimento  M.Wierup 《中国家禽》2011,33(5)

1 禽源沙门菌对公共卫生的影响 在许多国家,沙门菌仍是引发食源性肠道疾病的主要原因.沙门菌通常存在于家畜、家禽中,也可在啮齿类、爬行类和鸟类等野生动物中发现.当农场的禽群感染沙门菌后,通常会有一部分家禽的消化道中携带沙门菌,但并无症状.随后,沙门菌可能会通过粪便污染感染动物屠体.  相似文献   

Cross comparison of seminal plasma proteins from cattle and buffalo (Bubalus bubalis)     

Viviane Maria Codognoto  Paulo Henrique Yamada  Rúbia Alves Schmith  Felipe Rydygier de Ruediger  Camila de Paula Freitas-Dell’Aqua  Fabiana Ferreira de Souza  Suzane Brochine  Lucas Monteiro do Carmo  Andressa Filaz Vieira  Eunice Oba 《Reproduction in domestic animals》2020,55(1):81-92

The objective of this study was to evaluate seminal plasma proteins from cattle and buffalo (Bubalus bubalis), to identify differences between related species. Sixteen buffaloes and 16 cattle between 30 and 60 months of age were used. Semen collection was performed by electroejaculation, followed by macroscopic and microscopic subjective analyses. After analysis, the samples were centrifuged at 800 g for 10 min, and the supernatant (seminal plasma) was recentrifuged at 10,000 g for 30 min at 4°C. The total protein concentration was determined by the Bradford method, and the proteins were digested in solution for mass spectrometry (nLC-MS/MS). Multivariate statistical analysis was used to evaluate the proteomics results by non-hierarchical clustering the considering exponentially modified protein abundance index (emPAI). Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were used for clustering. Proteomics identified 78 proteins, and multivariate analysis showed 4 that were over-expressed in buffaloes (cystatin C, prosaposin, peptide YY and keratin type II cytoskeletal 5) and 9 in cattle (spermadhesin-1, seminal plasma protein PDC-109, ribonuclease 4, metalloproteinase inhibitor 2, acrosin inhibitor 1, seminal ribonuclease, C-type natriuretic peptide, angiogenin-1 and osteopontin). Among the proteins identified in seminal plasma, the C-type natriuretic peptide and metalloproteinase inhibitors were described for the first time in buffaloes. Some protease inhibitors were found over-expressed in buffaloes, and important proteins in seminal plasma of cattle were not identified or were found at lower expression levels in buffaloes, which can contribute to reproductive performance in this species.  相似文献   

Ruminal and histological characteristics and nitrogen balance in lamb fed diets containing cactus as the only roughage     

da Silva  Kleitiane Balduino  de Oliveira  Juliana Silva  Santos  Edson Mauro  Cartaxo  Felipe Queiroga  Guerra  Ricardo Romão  do Nascimento de Souza  Aelson Fernandes  Muniz  Ana Cecília Souza  de Lima Cruz  Gabriel Ferreira 《Tropical animal health and production》2020,52(2):637-645

Tropical Animal Health and Production - This study examined rumen fermentation, histological, blood, and urinary characteristics as well as ammoniacal nitrogen concentration, pH, nitrogen balance,...  相似文献   

Biofortification of soybean grains with foliar application of Li sources     

Antonio Carlos Martins dos Santos  Kleycianne Ribeiro Marques  Larissa Urzêdo Rodrigues  Álvaro José Gomes de Faria  Vitor L. Nascimento 《Journal of plant nutrition》2013,36(19):2522-2531

Abstract

Biofortification of soybean grains with lithium (Li) is a strategy to improve a food with high social acceptance, in order to promote health benefits. The aim of this work was to evaluate the development of plants and the production of biofortified soybeans with two Li sources. The experiment was conducted in a completely randomized design with five replicates. Ten treatments were obtained in a 2x5 factorial scheme, comprising two sources of Li (LiOH - Li hydroxide and Li₂SO₄ - Li sulfate) and five doses (0, 30, 60, 90 and 120?mg kg^?1) in stages V4 and R1. The supply of Li promoted significant effects (p?≤?0.05) on the morphology, yield components and nutritional status of soybean plants. The highest grain yield was obtained with the use of Li₂SO₄ at the estimated dose of 45.7?mg kg^?1. The plants accumulated Li between 8.00 and 11.20?mg kg ^?1, respectively, with the use of Li₂SO₄ and LiOH. In the grains, the highest concentrations of Li were obtained with the application of 120?mg kg^?1. Li₂SO₄ and LiOH are good sources for biofortification of soybean grains.  相似文献   

Responses of biomass and several photosynthetic indicators to manganese excess in triticale     

V. Quartin  J. C. Ramalho  M. A. Nunes 《Journal of plant nutrition》2013,36(8):1615-1629

Triticale (X Triticosecale, Wittmack) is generally accepted as a very rustic crop which can grow in difficult soils such as those with low pH, where aluminum (Al) and/or manganese (Mn) are frequently in excess. The first aim of the present work was to establish the parameters for the future evaluation of Mn excess tolerance. Plants were grown in pots with sand fed with a nutrient solution (pH 4.5) to which MnSO₄ .4H₂O was added. The biomass of plants (cv. Arabian) grown during one monm in a range of Mn concentrations (2,2.75,11, 16.5,22, 33, and 44 ppm) was determined in Experiment I. Subsequently a shorter range (10, 50, 100, and 200 ppm) was chosen and used with four cvs. (Arabian, Beagle, Borba, and TTE 9201) during the complete growth period (Experiment II). During this experiment the effects of Mn concentrations on the net photosynthetic rates, stomatal conductance, photosynthetic capacity, photochemical efficiency of PSII, quantum yield of photosynthetic non cyclic electron transport and leaf content of chlorophylls, and carotenoids in the several cvs. were studied. The final biomass and yield were taken as the ultimate criteria for ranking the cvs. It was concluded that 10 ppm Mn concentration was optimal for the growth and 50 ppm the lowest limit to produce toxicity which indicates that triticale was rather tolerant. All the physiological parameters determined after one month of growth and the performance of the photosystems were negatively affected by the Mn concentrations, but the changes were not sufficient to be used for an early (one month old plants) ranking of the cvs. consistent with the biomass and final grain yield. However, all the results agree that the cv. Arabian was the most sensitive because it suffered the strongest decreases and was affected by 50 ppm Mn concentration. The plants of the cv. TTE 9201 were the least affected as far as photosynthetic parameters are concerned.  相似文献   

Race identification of Fusarium oxysporum f. sp. lycopersici isolates obtained from tomato plants in Nova Friburgo,Brazil     

de Oliveira  Cristiana Maia  do Carmo  Margarida Gorete Ferreira  Ferreira  Leandro Martins  Höfte  Monica  do Amaral Sobrinho  Nelson Moura Brasil 《European journal of plant pathology / European Foundation for Plant Pathology》2021,161(2):273-287

European Journal of Plant Pathology - Fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici (FOL) is one of the main diseases affecting tomato plants. Three races (races 1, 2 and 3) of the...  相似文献   

Trichoderma as a biological agent of Fusarium oxysporum species complex and Vigna unguiculata growth promoter     

do Amaral  Ana Cl&#;udia Ten&#;rio  de Holanda Cavalcanti Maciel  Mar&#;lia  Machado  Alexandre Reis  de Oliveira  Luciana Gon&#;alves  Lima  Cristiano Souza  da Costa  Antonio F&#;lix  de Oliveira  Neiva Tinti 《European journal of plant pathology / European Foundation for Plant Pathology》2022,163(4):875-890

European Journal of Plant Pathology - This study aimed to evaluate the potential antagonistic role of Trichoderma in the control of Fusarium isolates belonging to the Fusarium oxysporum species...  相似文献   

Evaluation of Cymbopogon flexuosus and Alpinia zerumbet essential oils as biopesticides against Callosobruchus maculatus     

Barbosa  Douglas Rafael e Silva  dos Santos  Rebeca Barbosa Vieira  Santos  Flavia Mayara Pereira  da Silva Junior  Pedro Justino  de Oliveira Neto  Francisco Marques  Silva  Gutierres Nelson  de Andrade Dutra  Kamilla  do Amaral Ferraz Navarro  Daniela Maria 《植物病害和植物保护杂志》2022,129(1):125-136

Journal of Plant Diseases and Protection - This study aimed to investigate the effects of the essential oils of Cymbopogon flexuosus and Alpinia zerumbet in the treatment of cowpea [Vigna...  相似文献   

Temperature-dependent development rates of <Emphasis Type="Italic">Bracon vulgaris,</Emphasis> a parasitoid of boll weevil     

F. S. Ramalho  P. A. Wanderley  J. B. Malaquias  K. C. V. Rodrigues  J. V. S. Souza  J. C. Zanuncio 《Phytoparasitica》2009,37(1):17-25

The duration of development of Bracon vulgaris Ashmead, parasitoid of the boll weevil Anthonomus grandis Boheman, was determined at nine constant temperatures between 18°C and 38°C. Nonlinear regression analysis was used to test the fit of temperature-dependent development rates to the Sharpe and DeMichele and Lactin et al. models. At the highest tested temperature (38°C) all the parasitoid eggs died before hatching and no evidence of development was observed. The high values of R ² for the models of Sharpe and DeMichele (0.8432 to 0.9834), and Lactin et al. (0.9071 to 0.9795) indicated that these models are suitable to estimate the development rate of B. vulgaris as a function of temperature. B. vulgaris showed tolerance to high temperature which is represented by the high value of H _H (change in enthalpy associated with high-temperature inactivation of the enzyme) for the prepupa stage of this insect obtained with the Sharpe and DeMichele model. According to that model, B. vulgaris exhibits thermal stress at 35.7°C, which indicates that maximum thermal stress estimated by this model was close to the real one.  相似文献