Atypical sheep scab     

Otter A  Bates P 《The Veterinary record》2002,151(17):515-516

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Comparison of two heated gutta-percha obturation techniques of the mandibular first molar in the dog     

Ulbricht RD  Marretta SM  Baker GJ  Siegel AM  Klippert LS 《Journal of veterinary dentistry》2002,19(2):63-70

An in vitro study compared two heated gutta-percha obturation techniques of the mandibular first molar in the dog. Thirty hemimandibles were harvested from adult dog cadavers. The mandibular first molars were instrumented and obturated using either the ThermaFil Plus technique or the SuccessFil vertical compaction technique. Obturation times were recorded and three radiographic views were taken of all teeth for evaluation of the endodontic fill. Fifty-two of the mandibular first molar roots were evaluated in the apical dye leakage portion of the study. The ThermaFil Plus obturation technique required less time and provided a better radiographic endodontic fill, however there was significantly greater apical dye leakage in this technique compared with teeth that were obturated using the SuccessFil vertical compaction technique.  相似文献   

Plasma pyruvate kinase activity vs creatine kinase activity as an indicator of the porcine stress syndrome     

G G Duthie  J R Arthur  S P Simpson  F Nicol 《American journal of veterinary research》1988,49(4):508-510

Plasma pyruvate kinase (PK) and creatine kinase (CK) activities were increased significantly (P less than 0.001 and P less than 0.05, respectively) in homozygote halothane-reacting pigs (nn), compared with those activities in homozygote nonreacting pigs (NN). Pyruvate kinase activity was less variable within groups than was CK activity, allowing more effective discrimination between nn and NN geno-types. The PK and CK activities in plasma increase with age in halothane-reacting pigs and the nonreacting pigs. Enzyme activities in heterozygote (Nn) nonreacting pigs did not differ significantly (P greater than 0.05) from enzyme activities of homozygote (NN) nonreacting pigs. Although PK was better than CK in identifying stress-susceptible pigs, age-related effects and the failure to identify heterozygotes may restrict the use of plasma PK activity as a diagnostic test for the stress syndrome.  相似文献   

Blood antioxidant status and plasma pyruvate kinase activity of halothane-reacting pigs   总被引：2，自引：0，他引：2  

G G Duthie  J R Arthur 《American journal of veterinary research》1987,48(2):309-310

Samples of blood were collected (by jugular venipuncture) from 10 homozygote halothane-reacting pigs and 7 homozygote nonreactors. Antioxidant status was assessed by measurement of blood glutathione peroxidase, glucose-6-phosphate dehydrogenase, and superoxide dismutase activities and plasma concentrations of vitamin E. Significant differences in these values were not apparent between halothane-reacting pigs and nonreactors. Halothane-reacting pigs had tissue damage as indicated by significantly increased plasma activities of the enzymes creatine kinase and pyruvate kinase. The latter showed better discrimination between the 2 populations than did creatine kinase and could prove to be a more effective marker for identification of stress-susceptible pigs.  相似文献   

硒缺乏破坏动物褐脂肪的非寒颤性产热功能     

呙于明 Arth.  JR 《畜牧兽医学报》1994,25(3):201-205

硒缺乏导致大鼠肝脏，褐脂肪等组织的谷胱甘肽过氧化物酶活性极显著，大幅度下降，而肝脏谷胱甘肽硫转移酶活性升高了１．４５倍。各组织ＧＰＸ及ＧＳＴ活性不受冷应激的影响；冷应激导致肩胛间褐脂肪组织重量和其中的５＇－脱碘酶活性分别大幅度下降和上升，硒营养缺乏明显减小变化的幅度：正常大鼠每毫克鲜褐脂肪组织的５＇－脱碘酶活性经冷应激后上升了４６倍，组织重量下降了１６．７％；而缺硒组的只上升了３．４倍，组织重量只  相似文献   

REPRODUCTIVE PERFORMANCE OF BEEF CATTLE IN NORTHWESTERN QUEENSLAND     

R. G. Holroyd  B. A. Arthur  † B. G. Mayer  ‡ 《Australian veterinary journal》1979,55(5):257-262

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Rates of entry of alanine and glycerol and their contribution to glucose synthesis in fasted chickens     

B Emmanuel  R Berzins  A R Robblee 《British poultry science》1983,24(4):565-571

Rates of entry of alanine and glycerol and their contribution to glucose synthesis were studied in 48-h fasted White Leghorn cockerels using primed constant intravenous infusions of L-[U-14C]alanine and [U-14C]glycerol. Entry rates of alanine and glycerol were 112 and 401 mumol/h per kg body weight, respectively. Of the total glucose irreversible loss (entry rate), 1.5 and 6.6% were derived from alanine and glycerol, respectively. The possibilities of the operation of a glyoxylate cycle and omega-oxidation of fatty acids in the fasted chicken are discussed.  相似文献   

Ovine enzootic abortion: field observations on naturally acquired and vaccine-elicited delayed type hypersensitivity to Chlamydia psittaci (ovis)     

A J Wilsmore  M Dawson  C J Trower  C Venables  M J Arthur 《The Veterinary record》1986,118(12):331-332

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Quantitative analysis of RNA levels from single hepatocytes in vivo: combined use of real-time RT-PCR and laser microdissection     

SHI Xin  Jrg Kleeff  ZHU Zhao-wen  Bruno Schmied  TANG Wen-hao  Arthur Zimmermann  Markus W.Büchler  Helmut Friess△ 《园艺学报》2007,23(11):2285-2288

AIM: The manner in which a cell responds to and influences its environment is ultimately determined by the genes that are expressed.To better understand cellular functions,the isolation of single cells and subsequent quantification of the expressed genes is essential.METHODS: Normal liver tissue was obtained from operation,snap-frozen in liquid nitrogen and sectioned in crystat.Individual hepatocytes were microdissected.RNA was extracted,then reverse transcribed and amplified using real-time quantitative polymerase chain reaction (PCR).RESULTS: Single hepatocytes were dissected by laser beam and catapulted to the microcentrifuge cap which was put above the slide.In this way,cells were collected,RNA was extracted,reverse transcribed to cDNA and used for analysis of RNA expression by real-time quantitative PCR.The amplification results showed that quantitation of the RNA inside the cell was compatible with the number of cells.CONCLUSION: The expression of RNA in single cells can be quantitated successfully by using laser microdissection and real-time PCR.These techniques provide an opportunity to monitor in vivo gene expression levels in single hepatocytes.  相似文献   

Specific Behaviour of French Aphanomyces Euteiches Drechs. Populations For Virulence and Aggressiveness on Pea, Related to Isolates from Europe, America and New Zealand     

Emmanuel Wicker  Francis Rouxel 《European journal of plant pathology / European Foundation for Plant Pathology》2001,107(9):919-929

The pathogenic variability of Aphanomyces euteiches on pea was investigated using a collection of 88 pea-infecting isolates from France and 21 isolates from Denmark, Sweden, Norway, USA, Canada and New Zealand. Aggressiveness and virulence were assessed by scoring the root symptoms on a differential set of six pea genotypes. Eleven virulence types were characterised. The virulence type I, previously described as virulent on the whole set, was predominant and included the most aggressive isolates of all geographical origins. The other types were much less prevalent, existing as one to five isolates. Three virulence types (III, IV and V) contained no French isolates. The type III, avirulent on MN313, was composed of American isolates only, and resembled the major group recently described in the USA. A wide range of aggressiveness was found within the virulence type I, and the French isolates appeared globally more aggressive than the foreign isolates. These findings indicate that isolates from the virulence type I should be used as references in breeding programs, and that pea lines PI180693 and 552 may be the most interesting resistance sources to date, despite their only partial resistance.  相似文献