Characterization of Mycoplasma hyosynoviae strains by amplified fragment length polymorphism analysis,pulsed-field gel electrophoresis and 16S ribosomal DNA sequencing     

Kokotovic B  Friis NF  Ahrens P 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2002,49(5):245-252

Mycoplasma hyospnoviae strains from Denmark, Germany, Japan, Sweden, the Netherlands and the UK were examined for variations in the genomic DNA and within the 16S ribosomal RNA (rRNA) gene. Variations in the chromosomal DNA among 57 isolates recovered from the respiratory tract and joints of pigs, were investigated by analysis of amplified fragment length polymorphisms of the Bg/II and MfeI restriction sites and by pulsed-field gel electrophoresis of a BssHII digest of chromosomal DNA. Both methods allowed unambiguous differentiation of the analysed strains and showed similar discriminatory potential for the differentiation of M. hyosynoviae isolates. Concordant results obtained with the two whole-genome fingerprinting techniques evidence the considerable intraspecies genetic heterogeneity of M. hyosynoviae. Sixteen field strains of M. hyosynoviae and the type strain S16(T) were further examined for variation within the 16S rRNA gene. Ten field strains possessed the 16S rDNA sequences identical to the type strain, while the remaining six strains had sequences that differed by one to two nucleotides from that obtained from the type strain.  相似文献   

Saving large fish through harvest slots outperforms the classical minimum‐length limit when the aim is to achieve multiple harvest and catch‐related fisheries objectives     

Robert N. M. Ahrens  Micheal S. Allen  Carl Walters  Robert Arlinghaus 《Fish and Fisheries》2020,21(3):483-510

We address the problem of optimal size‐selective exploitation in an age‐structured fish population model by systematically examining how density and size dependency in growth, mortality and fecundity affect optimal harvesting patterns when judged against a set of fisheries objectives. The study offers five key insights. First, while minimum‐length limits often maximize the biomass yield, exploitation using harvest slots (i.e. regulations that protect both immature and very large individuals) can generate within 95% of maximum yield; harvest slots also generally maximize the number of fish that are harvested. Second, density dependence in growth and size‐dependent mortality predict more liberal optimal size limits than those derived under assumptions of no density and size dependence. Third, strong density dependence in growth maximizes the catch of trophy fish only when modest harvest is introduced; the same holds for numbers harvested, when the stock–recruitment function follows the Ricker type. Fourth, the inclusion of size‐dependent maternal effects on fecundity or egg viability has only limited effects on optimal size limits, unless the increase in fecundity with mass (“hyperallometry”) is very large. However, large hyperallometry in fecundity shifts the optimal size limit for biomass yield from the traditional minimum‐length limit to a harvest slot. Fifth, harvest slots generally provide the best compromises among multiple objectives. We conclude that harvest slots, or more generally dome‐shaped selectivity to harvest, can outperform the standard minimum‐length selectivity. The exact configuration of optimal size limits crucially depends on objectives, local fishing pressure, the stock–recruitment function, and the density and size dependency of growth, mortality and fecundity.  相似文献   

Comparative chromosomal studies of E. caballus (ECA) and E. przewalskii (EPR) in a female F1 hybrid     

E. Ahrens  & G. Stranzinger 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2005,122(S1):97-102

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Natural dust and acid rain     

Erhard M. Winkler 《Water, air, and soil pollution》1976,6(2-4):295-302

Atmospheric dust originates from three sources: terrestrial airborne matter, volcanic, and cosmic. Terrestrial natural dust makes up the main bulk reflecting the soil composition to 150 mi away. Soil erosion from flood plains, plowed fields and construction sites is the main source. Quartz, feldspar, the carbonates calcite and dolomite, and clay minerals are the components in decreasing order of frequency. Natural dust in the atmosphere interacts with rainwater converting the carbonates to benign gypsum (CaSO₄.2H₂0). Naturally leached soils produce less calcite than unweathered sediments on flood plains and construction sites, and in granitic and crystalline rocks less than in limestone areas. Heavy industrialization associated with high emission of C0₂ and S0₂ on the one hand, and excess production of dust on the other appears to counteract man's interference with natural ecosystems in the opposite direction.  相似文献   

A few thoughts on the legal evaluation of leukosis in meat inspection     

G Ahrens 《Monatshefte für Veterin?rmedizin》1965,20(22):921-925

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Development of a novel self-medicating applicator for control of internal and external parasites of wild and domestic animals     

Burridge MJ  Simmons LA  Ahrens EH  Naudé SA  Malan FS 《The Onderstepoort journal of veterinary research》2004,71(1):41-51

Four trials, three in the United States and one in South Africa, were conducted to evaluate the potential value of a novel self-medicating applicator in the passive control of gastrointestinal nematodes in cattle and deer, and of files and ticks on cattle using oil-based treatments. The results of the trials demonstrated that this applicator is an effective and practical device for the passive treatment of both deer and cattle for trichostrongyle infections using the endectocide, moxidectin (Cydectin. Fort Dodge Animal Health, USA), of cattle for hom fly (Haemotobia irritans) infestations using the insecticide, cyfluthrin (CyLence, Bayer AG, Germany) and of cattle for tick infestations (in particular Amblyomma hebraeum and Rhipicephalus appendiculatus) using the acaricides deltamethrin and amitraz (Delete All, Intervet, South Africa).  相似文献   

Efficacy of the classical swine fever (CSF) marker vaccine Porcilis Pesti in pregnant sows   总被引：7，自引：0，他引：7  

Ahrens U  Kaden V  Drexler C  Visser N 《Veterinary microbiology》2000,77(1-2):83-97

The efficacy of the classical swine fever (CSF) subunit marker vaccine Porcilis Pesti based on baculovirus expressed envelope glycoprotein E2 of CSF virus (CSFV) was evaluated in pregnant sows. Ten gilts were vaccinated with one dose of marker vaccine, followed by a second dose 4 weeks later. Four gilts remained unvaccinated and received a placebo at the same times. Thirty-three days after the second vaccination all animals were artificially inseminated. Neither local or systemic reactions nor an increase of body temperature were observed after vaccinations. All gilts showed a normal course of pregnancy. Thirty-five days after first vaccination all animals developed E2 specific neutralising antibodies with titres in the range of 5.0 and 7.5 log(2). No antibodies to CSFV-E(rns) were found in ELISA.On day 65 of gestation (126 days after the first immunisation) all sows were infected intranasally using 2ml (10(6.6) TCID(50)/ml) of the low virulent CSFV strain "Glentorf". After challenge in two of the unvaccinated control sows a slight transient increase of body temperature was observed, whereas leukopenia was demonstrated in all control animals. In addition all controls became viraemic. Vaccinations with the CSFV subunit vaccine protected the animals from clinical symptoms of CSF. In two sows a moderate decrease of leukocyte counts was detected on day 5 post infection. In contrast to the unvaccinated control sows in none of the vaccinated animals virus was isolated from the nasal swabs or the blood.Approximately 40 days after challenge all sows were killed and necropsy was done. The sows and their offspring were examined for the presence of CSFV in blood, bone marrow and different organs. No virus was found in any of the sows. In contrast, in all litters of the control sows CSFV was found in the blood as well as in the organ samples. Nine out of 10 litters of the vaccinated sows were protected from CSFV infection. Blood samples, lymphatic organs and bone marrow of these animals were all virologically negative. When sera were tested for CSFV-antibodies all sows had developed E(rns)-specific antibodies but no CSFV-specific antibodies were found in any of the progeny.It was concluded that vaccination with CSF subunit marker vaccine Porcilis((R)) Pesti protected 90% of the litters from viral infection when sows were challenged mid-gestation using the CSFV-strain "Glentorf".  相似文献   

First case report of ultrastructural cutaneous abnormalities in equine atopic dermatitis     

R. Marsella  C. JohnsonK. Ahrens 《Research in veterinary science》2014

Atopic dermatitis (AD) is a common skin disease that affects humans and animals. Skin impairment has been described in human and canine AD. Equine AD is recognized in practice but little is known about its pathogenesis. As remarkable similarities exist across species in terms of cutaneous manifestations of AD, it was speculated that skin abnormalities may also exist in atopic horses. This case report describes the ultrastructure of the stratum corneum of two normal and two atopic horses. Biopsies were taken from sites predisposed to AD and examined using electron microscopy. Stratum corneum in normal samples was compacted with organized lipid lamellae while in atopic samples disorganized lipid lamellae, retained lamellar bodies and amorphous lipids were found. These changes are very similar to what reported in AD in other species. It is currently unknown whether these abnormalities in atopic horses are primary or secondary and their importance in allergen penetration.  相似文献   

Oblique impact: a process for obtaining meteorite samples from other planets     

O'keefe JD  Ahrens TJ 《Science (New York, N.Y.)》1986,234(4774):346-349

Cratering flow calculations for a series of oblique to normal (10 degrees to 90 degrees ) impacts of silicate projectiles onto a silicate halfspace were carried out to determine whether or not the gas produced upon shock-vaporizing both projectile and target material would form a downstream jet that could entrain and propel SNC meteorites from the Martian surface. The difficult constraints that the impact origin hypothesis for SNC meteorites has to satisfy are that these meteorites are lightly to moderately shocked and yet have been accelerated to speeds in excess of the Martian escape velocity (more than 5 kilometers per second). Two-dimensional finite difference calculations were performed that show that at highly probable impact velocities (7.5 kilometers per second), vapor plume jets are produced at oblique impact angles of 25 degrees to 60 degrees and have speeds as great as 20 kilometers per second. These plumes flow nearly parallel to the planetary surface. It is shown that upon impact of projectiles having radii of 0.1 to 1 kilometer, the resulting vapor jets have densities of 0.1 to 1 gram per cubic centimeter. These jets can entrain Martian surface rocks and accelerate them to velocities greater than 5 kilometers per second. This mechanism may launch SNC meteorites to earth.  相似文献   

Geo‐Pedogenese mariner Rohmarschen Spiekeroogs     

Luise Giani  Vera Ahrens  Oliver Duntze  Sven Kruse Irmer 《植物养料与土壤学杂志》2003,166(3):370-378

Geo‐pedogenesis of Salic Fluvisols on the North Sea island of Spiekeroog Although the mainland's Salic Fluvisols of the southern North Sea coast are rich in carbonate, those of the adjacent island Spiekeroog are not. Thus, the aim of this study was to investigate the Salic Fluvisols of Spiekeroog in comparison to those of the mainland and to find out the reasons for the lack of carbonate, which was supposed to be an interaction between sedimentation and sulfur dynamics. The vegetation, many physical and chemical properties (e.g. pH, C_org, salinity, Fe_od, exchangeable cations, CEC) of the Spiekeroog Fluvisols are similar to those of the mainland. However, the Spiekeroog soil profiles extended over the marsh sediments, and the sand sediments below and they contained more silt and less clay and carbonate (0.3 – 34.5 g kg^–1) than the soils of the mainland. A survey proved carbonate‐free areas. The relatively moderate S dynamics showed a spatial and temporal stabile SO₄‐S‐fraction (0.3 – 0.6 mg g^–1), a fairly stabile HNO₃‐HCl‐S fraction (1.5 – 2.9 mg g^–1), but a variable HCl‐S fraction. The vertical Cs‐137 distribution showed peaks at 3 – 4 and 7 – 8 cm soil depth, indicating sedimentation rates of 0.2 – 0.5 cm a^–1. The results showed that moderate S dynamics lead to carbonate losses which are not compensated because of low sedimentation rates. Consequently, the Spiekeroog Salic Fluvisols will enter the terrestrial phase of soil development without carbonate. Thus, besides the usual pedogenesis of Eutric Fluvisols via Salic and Calcaric Fluvisols, the investigation proved a direct development from Salic to Eutric Fluvisols.  相似文献