Comparison of methods for routine separation of coagulase-negative staphylococci from micrococci isolated from sheep     

Ricardo de la Fuente  Jesus Almazan  Esperanza Gomez-Lucia  Jean Freney  Guillermo Suarez 《Comparative immunology, microbiology and infectious diseases》1986,9(4):347-353

A total of 176 Gram-positive, catalase positive cocci strains, isolated from sheep were studied by different routine tests for the differentiation of staphylococci and micrococci, comparing their validity and usefulness. By glucose fermentation and growth in the anaerobic portion of thioglycolate 85 and 73.6% respectively of coagulase negative staphylococci were misclassified as Micrococcus spp. Susceptibility to lysostaphin was an adequate test for the differentiation of the strains. Atypical results in the production of acid from glycerol/erythromycin were obtained in 11.8% of the coagulase negative strains and 16.7% of micrococci. The combined use of the selective media furazolidone agar and Schleifer and Krämer medium resulted in a fast and useful separation of ovine staphylococci and micrococci. The bacteriolytic activity misclassified 32.2% of the coagulase negative strains.  相似文献   

Coinfection by Cryptosporidium  parvum and Porcine Circovirus Type 2 in Weaned Pigs     

A. Núez  F. McNeilly  A. Perea  P. J. Snchez‐Cordn  B. Huerta  G. Allan  L. Carrasco 《Zoonoses and public health》2003,50(5):255-258

Routine histopathological diagnosis of one representative 3‐month‐old pig from a group suffering from diarrhoea revealed a massive degree of parasitation by Cryptosporidium parvum, with a concomitant infection by porcine circovirus type 2 (PCV2), that was confirmed by immunohistochemical procedures. The areas of intestine where parasites were more numerous presented abundant PCV2 infected cells in mucosa and submucosa. The concurrence of C. parvum, a rare primary intestinal pathogen in post‐weaning and growing pigs, and PCV2 infections suggest an increased susceptibility as a result of an immunosuppression state.  相似文献   

Antibodies against viral pathogens in dairy cattle in Colombia     

I. B. Griffiths  M. I. Gallego M.  Olga Marino J.  Esperanza De Clavijo  Irma Diaz 《Tropical animal health and production》1983,15(4):214-214

  相似文献   

Isolation of free-living pathogenic amoebae from thermal spas in N.W. Spain     

Monica Penas-Ares  Esperanza Paniagua-Crespo  Rafael Madriñan-Choren  Margarita Marti-Mallen  Maria Cristina Arias-Fernandez 《Water, air, and soil pollution》1994,78(1-2):83-90

We tested for the presence of free-living amoebae in water from 12 thermal spas in northwest Spain; 8 tested positive. No relationship was observed between temperature, pH or bacterial contamination and either the presence of amoebae or the, number of strains per sample. A total of 13 strains were found: 5 of Vahlkampfia, 2 of Naegleria, 3 of Acanthamoeba and 3 of other genera. Those Naegleria and Acanthamoeba strains which survived at 37 ? were tested for pathogenicity by intracerebral and intranasal inoculation of mice; only 2 strains (both of the species Acanthamoeba polyphaga) caused death.  相似文献   

Influence of Dehydration Process in Castellano Chickpea: Changes in Bioactive Carbohydrates and Functional Properties     

Aguilera Y  Benítez V  Mollá E  Esteban RM  Martín-Cabrejas MA 《Plant foods for human nutrition (Dordrecht, Netherlands)》2011,66(4):391-400

Changes in bioactive carbohydrates, functional, and microstructural characteristics that occurred in chickpea under soaking, cooking, and industrial dehydration processing were evaluated. Raw chickpea exhibited important levels of raffinose family of oligosaccharides (RFOs), resistant starch (RS) and total dietary fibre (TDF), being insoluble dietary fibre (IDF) the main fraction (94%). The dehydration process increased RFOs (43%), RS (47%) and soluble dietary fiber (SDF) (59%) levels significantly. In addition, a noticeable increase in both fibre fractions was observed, being higher in soluble fibre in (SDF) (59%). The minimum nitrogen solubility of raw flours was at pH 4, and a high degree of protein insolubilization (80%) was observed in dehydrated flours. The raw and processed flours exhibited low oil-holding capacities (1.10 mg/ml), and did not show any change by thermal processing, whereas water-holding capacities rose to 5.50 mg/ml of sample. Cooking and industrial dehydration process reduced emulsifying activity and foaming capacity of chickpea flour. The microstructural observations were consistent with the chemical results. Thus, the significant occurrence of these bioactive carbohydrate compounds along with the interesting functional properties of the dehydrated flours could be considered useful as functional ingredients for food formulation.  相似文献   

Effects of different land use on soil chemical properties, decomposition rate and earthworm communities in tropical Mexico   总被引：1，自引：0，他引：1  

Violette Geissen  Karina Pea-Pea  Esperanza Huerta 《Pedobiologia》2009,53(1):75-86

The effects of land use on soil chemical properties were evaluated, and earthworm communities and the decomposition rate of three typical land use systems in tropical Mexico, namely banana plantations (B), agroforestry systems (AF) and a successional forest (S) were compared.The study was carried out from November 2005 to April 2006. A completely randomized sampling design was established in six sites (B1, B2, AF1, AF2, S1 and S2). Soil properties and chemical characteristics (texture, pH, organic carbon (Corg), nutrients, and available Zn and Mn), earthworm communities and the decomposition of Bravaisia integerrima and Musa acuminata litter were analyzed over a period of 8 weeks.All soils were loamy clays with a medium to high content of nutrients. Three principal clusters were generated with the soil chemical properties: a first cluster for forest soils with high Corg and Ntot and low available Zn content, a second cluster for AF1 and a third cluster for B1, B2 and A2.The decomposition of B. integerrima litter was significantly faster (half-life time: 1.8 (AF2)–3.1 (B1) weeks) than that of M. acuminata (4.1 (AF2)–5.8 (S2) weeks). However, the decomposition rates did not differ significantly among the different sites.The greatest earthworm diversities were observed in AF2 and B1. Native species were dominant in the forest soils, whereas exotic species dominated in AF and in the banana plantations. The abundance and biomass of certain earthworm species were correlated to physical and chemical soil parameters. However, litter decomposition rates were not correlated with any of the soil physical–chemical parameters.While none of the land use systems studied led to a decrease in nutrient status, earthworm biodiversity and abundance, or in litter decomposition rate, they did result in a change in earthworm species composition.  相似文献   

Sources of the highly expressed wheat bread making (wbm) gene in CIMMYT spring wheat germplasm and its effect on processing and bread-making quality     

Carlos Guzmán  Yonggui Xiao  Jose Crossa  Héctor González-Santoyo  Julio Huerta  Ravi Singh  Susanne Dreisigacker 《Euphytica》2016,209(3):689-692

  相似文献   

Pedodiversity in mountainous tropical semideciduous forests of Sierra Madre del Sur,Mexico     

P. V. Krasilnikov  N. E. Garcia Calderon  A. Ibañez Huerta 《Eurasian Soil Science》2009,42(13):1435-1442

We studied pedodiversity in mountainous tropical semideciduous forests at three key sites in Sierra Madre del Sur, Southern Mexico, at the altitudes from 600 to 1400 m a.s.l. Using detailed soil maps, we recorded the total number and the areas of soil delineations at each site, and calculated the pedodiversity indices. We found that pedodiversity increases with an increase in the absolute height within the zone of semideciduous forests, and interpreted this phenomenon as an evidence for a smaller development of erosion at higher altitudes. The areas of soil delineations reach their minimum within the intermediate part of this zone, because large delineations of strongly weathered clayey soils are developed at the higher altitudes, and large delineations of recent soils on strongly eroded surfaces are found at the lower altitudes. A conclusion about the need to take into account the particular composition of the soil cover upon the interpretation of pedodiversity data is made.  相似文献   

Detection of Feline leukemia virus in the endangered Iberian lynx (Lynx pardinus).   总被引：2，自引：0，他引：2  

Inés Luaces  Ana Doménech  Marino García-Montijano  Victorio M Collado  Celia Sánchez  J German Tejerizo  Margarita Galka  Pilar Fernández  Esperanza Gómez-Lucía 《Journal of veterinary diagnostic investigation》2008,20(3):381-385

Feline retroviruses are rarely reported in lynx species. Twenty-one Iberian lynx (Lynx pardinus) blood and tissue samples collected from Do?ana National Park and Los Villares (Sierra Morena) in southern Spain during 1993-2003 were analyzed by polymerase chain reaction to amplify nucleic acids from feline retroviruses. Six samples were positive for Feline leukemia virus (FeLV), but no samples tested positive for Feline immunodeficiency virus. The BLAST analysis indicated that 5 of the 6 sequences were closely related to FeLV strain Rickard subgroup A, whereas 1 sequence was identical to FeLV. To the authors' knowledge, this is the first report of FeLV in the endangered Iberian lynx.  相似文献   

Retrospective study on swine Torque teno virus genogroups 1 and 2 infection from 1985 to 2005 in Spain   总被引：3，自引：0，他引：3  

Segalés J  Martínez-Guinó L  Cortey M  Navarro N  Huerta E  Sibila M  Pujols J  Kekarainen T 《Veterinary microbiology》2009,134(3-4):199-207

  相似文献