排序方式： 共有53条查询结果，搜索用时 15 毫秒
51.
Laura Cutillas-Barreiro David Fernández-Calviño Avelino Núñez-Delgado María J. Fernández-Sanjurjo Esperanza Álvarez-Rodríguez Juan Carlos Nóvoa-Muñoz Manuel Arias-Estévez 《Water, air, and soil pollution》2017,228(7):260
The establishment of a complementary grass cover on vineyard soils can promote sustainability of the affected environment. In this work, we used an acid vineyard soil with total Cu concentration 188 mg kg?1 to study the influence of pine bark amendment on Lolium perenne growth and Cu uptake. The results indicate that the pine bark amendment did not cause a significant increase in the mass of the shoots of Lolium perenne, but favored the root biomass: 0.034 g for control and 0.061 g for soil samples amended with 48 g kg?1 of pine bark. Moreover, the pine bark amendment decreased Cu concentration in both, shoots (50 mg kg?1 for control soil and 29 mg kg?1 for soil amended with 48 g kg?1 pine bark) and roots (250 mg kg?1 for control soil and 64 mg kg?1 for soil amended with 48 g kg?1 pine bark). The main factor responsible for these results was a significant decrease of the most mobile fractions of Cu in the soil. Those fractions were extracted using ammonium acetate, ammonium chloride, sodium salt of ethylene-diamine-tetraacetic acid (EDTA-Na), and diethylene-triamine-pentaacetic acid (DTPA). 相似文献
52.
Arjona A Barquero N Doménech A Tejerizo G Collado VM Toural C Martín D Gomez-Lucia E 《Journal of Feline Medicine and Surgery》2007,9(1):14-22
Laboratory diagnosis of feline leukemia virus (FeLV) and feline immunodeficiency virus (FIV) usually involves both viruses, as the clinical signs are similar and coinfection may occur. Serological methods may not represent an accurate diagnosis: maternal antibodies or cross-reactions may give false positive results to FIV, and false negative results may occur in latent FeLV status, or in certain FIV infection stages. A nested polymerase chain reaction (PCR) technique was designed to detect FeLV, FIV and feline endogenous retrovirus simultaneously. The detection of endogenous sequences was considered indicative of successful DNA extraction. The technique was used to diagnose FIV and FeLV in the blood cells of 179 cats. The kappa value with the serological data was 0.69 for FeLV and 0.87 for FIV. The joint detection of FeLV and FIV by this novel nested PCR is sensitive, specific, fast and convenient, and its applicability for clinical diagnosis is promising, as the direct evidence of the presence of the virus is more realistic than the indirect data provided by the serological detection. 相似文献
53.
Ariadna Sitjà-Bobadilla Maria Jesus Pujalte Azucena Bermejo Esperanza Garay Pilar Alvarez-Pellitero & Jaume Pérez-Sánchez 《Aquaculture Research》2007,38(7):733-739
A pathological study was conducted on laboratory‐reared juvenile common dentex (Dentex dentex) suffering trickling and continuous mortalities. During a 3‐month period (October–December), water temperature, clinical signs and mortalities were recorded. Moribund or dead fish were examined for bacteria and parasites. Bacteria were isolated from head kidney and external ulcers, and samples from the gills, intestine, stomach, trunk kidney, gall bladder and liver were taken for histology. Cumulative mortality reached 73%, and 80% of fish examined were positive for bacteria (102 isolates). Vibrio splendidus was the most prevalent in head kidney (59.7%) and ulcers (88.9%), and it was frequently isolated as pure culture (74.7% and 100% respectively). This is the first report of this bacterium in association with mortality in common dentex. Vibrio scophthalmi was the second most prevalent bacterium (29.2%) and accounted for 63.6% of the mixed infections with V. splendidus, V. harveyi, Pseudoalteromonas spp. and other species were rarely isolated. No parasites were found in histological sections. Bacteria were frequently observed in the lumen of the intestine and stomach, destroying the epithelium. A cellular reaction was suggested by the high numbers of rodlet cells (RC) in the intestinal epithelium, and the abundance of eosinophilic granular cells (EGC) in the intestinal lamina propria. In the stomach, vacuolized cells containing unidentified debris, sometimes of crystalloid appearance, were very common. The high prevalence of V. splendidus in pure cultures and the absence of other aetiological agents suggest that V. splendidus is substantially involved in the registered mortalities, although the implication of V. scophthalmi and even of some nutritional factors cannot be discounted. 相似文献