Molecular cloning and characterization of Gossypium hirsutum superoxide dismutase genes during cotton–Xanthomonas campestris pv. malvacearum interaction     

Andreas E. Voloudakis  Philippe Marmey  Etienne Delannoy  Aida Jalloul  Christelle Martinez  Michel Nicole 《Physiological and Molecular Plant Pathology》2006,68(4-6):119-127

  相似文献   

Isolation of Staphylococcus schleiferi subspecies coagulans from two cases of canine pyoderma     

Bes M  Guérin-Faublée V  Freney J  Etienne J 《The Veterinary record》2002,150(15):487-488

  相似文献   

Rate of carbon dioxide production and energy expenditure in fed and food-deprived adult dogs determined by indirect calorimetry and isotopic methods     

Pouteau EB  Mariot SM  Martin LJ  Dumon HJ  Mabon FJ  Krempf MA  Robins RJ  Darmaun DH  Naulet NA  Nguyen PG 《American journal of veterinary research》2002,63(1):111-118

OBJECTIVE: To evaluate energy expenditure (EE) in dogs by estimating rate of CO2 production (rCO2). ANIMALS: 15 Beagles. PROCEDURE: Food was withheld for 24 hours, and all dogs received an IV infusion of 13C sodium bicarbonate for 8 hours. Breath samples were collected before infusion and at 30-minute intervals from 4 to 8 hours, and 13C enrichment in breath CO2 was measured, using gas chromatography-isotopic ratio mass spectrometry. Food was withheld from 6 dogs, and rCO2 and O2 consumption were measured, using a conventional indirect calorimeter. The CO2 production and O2 consumption were measured by use of indirect calorimetry in 6 other fed dogs that were injected with 2H2O and H2(18)O. Blood samples were collected before tracer injection, 4 hours later, and on days 4, 7, and 11. Deuterium and 18O enrichments in plasma water were determined. RESULTS: Mean rCO2 measured by indirect calorimetry was 516 +/- 34 and 410 +/- 16 micromol/kg(0.75)/min in 6 fed and 6 food-deprived dogs, respectively. The rCO2 calculated from 13C-bicarbonate dilution was 482 +/- 30 micromol/kg(0.75)/min. Mean rCO2 determined by use of the double-labeled water method was 1,036 +/- 46 mmol/kg(0.75)/d. Mean energy expenditure calculated from rCO2 determined by infusion of 13C bicarbonate, indirect calorimetry in fed and food-deprived dogs, and infusion of double-labeled water was 386 +/- 39, 379 +/- 25, 338 +/- 14, and 552 +/- 25 kJ/kg(0.75)/d, respectively. CONCLUSIONS AND CLINICAL RELEVANCE: Energy expenditure calculated by indirect calorimetry in unfed dogs can be considered representative of basal metabolic rate.  相似文献   

ECG of the month. Second-degree atrioventricular (AV) block,type II advanced (3:1), with ventricular escape complexes     

Fife WD  Côté E 《Journal of the American Veterinary Medical Association》2002,220(2):172-173

  相似文献   

Isolation and characterization of efficient isoxaben-transforming Microbacterium sp strains from four European soils     

Arrault S  Desaint S  Catroux C  Sémon E  Mougin C  Fournier JC 《Pest management science》2002,58(12):1229-1235

Nutrient-agar plates containing isoxaben (500 mg litre(-1)) were used to isolate isoxaben-metabolising bacteria from four European soils incubated with the herbicide under laboratory conditions. In flask experiments, inoculation of a basal salts medium containing nitrogen and [phenyl-U-14C]isoxaben with an isolate (B2b) resulted in 33% recovery of the initial radioactivity as [14C]carbon dioxide after 2 weeks. A major metabolite identified by GC-MS and NMR analysis as 3-(1-ethyl-1-methylpropyl)isoxazol-5-ylamine accumulated both in basal salts and nutrient broth media. 2,6-Dimethoxybenzoic acid, a suspected metabolite of isoxaben, was not detected in either liquid media. However, the capability of the B2b isolate to use 2,6-dimethoxybenzoic acid as a source of carbon was demonstrated. Soil inoculation with the B2b strain resulted in an increase in the recovery of [14C] carbon dioxide from both [phenyl-U-14C] and [isoxazole-5-14C]isoxaben. The metabolite identified as 3-(1-ethyl-1-methylpropyl)isoxazole-5-ylamine only accumulated if the soil was autoclaved before inoculation. This metabolite was rapidly mineralized by the microflora of a natural soil without history of isoxaben treatment. Homology patterns of sequenced 16S rDNA between isoxaben-transforming isolates and reference strains showed that the four isolates identified belonged to the genus Microbacterium.  相似文献   

遮光处理对容器育柑桔幼苗生长与微环境的影响   总被引：2，自引：0，他引：2  

彭水宏  EtienneRabe 《果树学报》1998,(4)

以德尔塔（Delta）甜检和中熟娜特（Midknight）甜橙两个品种为材料,探讨不同颜色遮光网、遮光强度与遮光时间对容器育柑桔幼苗生长、水分利用、叶片净CO2代谢速率以及幼苗生长微环境如土壤温度、大气湿度的影响。结果表明：与对照相比,所有遮光处理的幼苗较高,茎干较粗,德尔塔甜橙的试验结果比中熟娜特甜橙明显;遮光5个月比遮光12周的处理效果更明显;不遮光处理利用较多的水分．途光处理间无明显差别;不这光处理的土壤温度在14：00显著高于所有遮光处理;各处理间叶片净CO2代谢速率和大气湿度无差别。  相似文献   

Estimation of sow milk nutrient output   总被引：5，自引：0，他引：5  

J Noblet  M Etienne 《Journal of animal science》1989,67(12):3352-3359

Ten replicates of two littermate gilts were used during a 21-d lactation in order to calculate relationships between milk nutrient intake and piglet growth rate and composition of gain. Gilts were fed 14.2 or 10.4 Mcal ME/d and litter size was standardized to 9 or 10 piglets. Piglets had no access to creep feed. Milk production was measured on 10 sucklings over 12 h on d 1, 5, 9, 13, 17 and 21 by the weigh-suckle-weigh method. Heat production of the piglets was measured (RQ method) on the same days in a confinement chamber. Milk composition was determined on the days following milk production measurements. Four to ten piglets/litter were slaughtered at weaning and their body composition was determined. Milk nutrient production during part of lactation was related closely to piglet weight gain and body weight (R2 = .80 to .96). Milk DM, energy and N output over the entire lactation were predicted from piglet ADG (R2 = .87 to .90) when, for each litter, the difference between energy in piglet daily weight gain measured by the slaughter technique and energy in piglet daily weight gain estimated by the RQ method was included in the model. This variable corrects for milk production measurement errors. The relationships were slightly improved, especially for energy output, when the composition of piglet weight gain was taken into account (R2 = .93 to .97).  相似文献   

Nitrogen balance in lactating sows fed on diets containing soybean oil meal or horsebean (Vicia faba) as a protein concentrate     

M. Etienne  P.H. Duee  B. Pastuszewska 《Livestock Production Science》1975,2(2):147-156

An experiment was carried out on multiparous sows to study the possibility of total substitution of soybean oil meal by whole horsebean in the lactation diet. Two groups of five sows were kept in metabolism crates during a 5-week long lactation, and their total excreta were collected for 5 days each week. The animals received a diet mainly composed of barley, in which the protein concentrate was soybean oil meal (“soybean” group), or whole horsebean (“horsebean” group) supplemented with d-l methionine (Table I). The two diets were isonitrogenous (14% protein). Milk production and gros composition were estimated at different stages of lactation. The amino acid content of the milk and the plasma level of free amino acids were determined around day 21 of lactation.The performance of the two groups of sows were similar (Table II): the litters were equalized at farrowing, the number of piglets weaned (8.8) and their mean weight at different stages of lactation were identical. Milk production, composition (Table III) and amino acid content (Table IV), and the output of milk constituents were similar. There was some tendancy for the apparent protein digestibility of the “soybean” diet to be higher (81.5% vs 79.6%), partly due to the greater digestibility of dry matter (82.3% vs 81.8%) caused by the lower crude fibre content of this meal. The nitrogen retention coefficient and nitrogen production in the milk were the same in the two groups of sows (Table V). The amino acid availability, estimated with the plasma level of free amino acids, did not show important differences between the diets. The similarity of the results allows the conclusion that whole horsebean supplemented with d-l methionine can be used as the sole protein concentrate in the diets of lactating sows. Moreover, the null nitrogen balances obtained during lactation with a daily intake of 700 g protein may lead to a possible lowering of the protein recommendations, while insisting on the energy supply during the same period.  相似文献   

Staphylococcus aureus Panton-Valentine leukocidin causes necrotizing pneumonia     

Labandeira-Rey M  Couzon F  Boisset S  Brown EL  Bes M  Benito Y  Barbu EM  Vazquez V  Höök M  Etienne J  Vandenesch F  Bowden MG 《Science (New York, N.Y.)》2007,315(5815):1130-1133

  相似文献   

Identification of fish species after cooking by SDS-PAGE and urea IEF: a collaborative study     

Etienne M  Jérôme M  Fleurence J  Rehbein H  Kündiger R  Mendes R  Costa H  Pérez-Martín R  Piñeiro-González C  Craig A  Mackie I  Malmheden Yman I  Ferm M  Martínez I  Jessen F  Smelt A  Luten J 《Journal of agricultural and food chemistry》2000,48(7):2653-2658

A collaborative study, to validate the use of SDS-PAGE and urea IEF, for the identification of fish species after cooking has been performed by nine laboratories. By following optimized standard operation procedures, 10 commercially important species (Atlantic salmon, sea trout, rainbow trout, turbot, Alaska pollock, pollack, pink salmon, Arctic char, chum salmon, and New Zealand hake) had to be identified by comparison with 22 reference samples. Some differences in the recoveries of proteins from cooked fish flesh were noted between the urea and the SDS extraction procedures used. Generally, the urea extraction procedure appears to be less efficient than the SDS extraction for protein solubilization. Except for some species belonging to the Salmonidae family (Salmo, Oncorhynchus), both of the analytical techniques tested (urea IEF, SDS-PAGE) enabled identification of the species of the samples to be established. With urea IEF, two laboratories could not differentiate Salmo salar from Salmo trutta. The same difficulties were noted for differentiation between Oncorhynchus gorbuscha and Oncorhynchus keta samples. With SDS-PAGE, three laboratories had some difficulties in identifying the S. trutta samples. However, in the contrast with the previous technique, SDS-PAGE allows the characterization of most of the Oncorhynchus species tested. Only Oncorhynchus mykiss was not clearly recognized by one laboratory. Therefore, SDS-PAGE (Excel gel homogeneous 15%) appears to be better for the identification, after cooking, of fish such as the tuna and salmon species which are characterized by neutral and basic protein bands, and urea IEF (CleanGel) is better for the gadoid species, which are characterized by acid protein bands (parvalbumins). Nevertheless, in contentious cases it is preferable to use both analytical methods.  相似文献