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61.

• Introduction   

In order to predict the biomass of aerial components of maritime pine stands (Pinus pinaster Ait.), generalized allometric equations were developed using data collected from the southern and northern margins of its geographical area.  相似文献   
62.
Potent bacterial strains effective against the whitefly, Bemisia tabaci, nymphs (second instar), were isolated from tomato cultivated fields at Fayoum governorate, Giza, Egypt. Of 72 isolates, 12 with the most morphologically distinct-looking bacterial colonies were selected and named A1, A2, A3, A6, A7, A9, A12, A13, A107, B37, B45 and B100. All isolates were preliminarily identified as members of the genus Bacillus based on morphological, physiological and biochemical characteristics. When tested for their pathogenicity against Bemisia tabaci, the 12 isolates revealed varying efficiencies with isolates A1 and A9 being superior, exhibiting maximum mortality of 92.2 and 90.8% on day 10, respectively. Isolate A7 recorded the lowest percentage at 18.3%. Further genetic characterization of the 12 isolates was performed using inter simple sequence repeat (ISSR), randomly amplified polymorphic DNA (RAPD) and 16S rDNA gene sequencing analysis. RAPD and ISSR results confirmed each other. The combined ISSR and RAPD phylogenetic tree showed two major clusters. With 16S rRNA gene analysis, isolate A1 and A12 sequences recorded 100% identity with Bacillus thuringiensis, while isolates A7 and B100 showed 95.7% and 95.6% identity with Bacillus cereus and Bacillus sphaericus, respectively.  相似文献   
63.
An intracellular ATP-dependent Ca2+ pumping mechanism, distinct from mitochondrial Ca2+ accumulation, was identified within tilapia gill cells. Cell suspensions treated with 0.003% saponin, which selectively permeabilizes the plasma membrane, were used to characterize the Ca2+ sequentering mechanisms as endoplasmic reticulum and mitochondria and to determine the effect of Zn2+ on their Ca2+ storing activity. Of the Ca2+ taken up by the endoplasmic reticulum, 80% was released by IP3 (10 mol l–1). The Ca2+ pump of the endoplasmic reticulum was 2.5 times less sensitive to Zn2+ (IC50=0.05 nmol l–1) than was the mitochondrial uptake mechanism (IC50=0.20 nmol l–1). The results indicate that Ca2+ is stored predominantly within the endoplasmic reticulum at 0.1 mol l–1 and that this storing capacity is seriously attenuated by namomolar concentrations Zn2+.  相似文献   
64.
Fish Physiology and Biochemistry - The current study investigated how different fasting and refeeding regimes would impact Nile tilapia growth performance, histopathological examination, and gene...  相似文献   
65.
66.
Journal of Plant Diseases and Protection - Orobanche cumana Wallr. is an important new problem for sunflower crop in Tunisia. Little information is available on the behavior of sunflower to O....  相似文献   
67.
The purpose of this study was to examine the effect of some immunostimulants and probiotics on the survival rate, final weight, and disease resistance of overwintered tilapia fry. There were five treatments: T1 (control) fed a balanced diet (35% protein) without additives. Treatments 2 to 5 were fed diets supplemented with 4% garlic, 4 g/kg Echinacea, 4 g/kg Organic Green or 4 g/kg Vet-Yeast, respectively. Growth and resistance to disease challenge with Aeromonas hydrophila and Pseudomonas flourescens were not different among treatments, but survival of overwintered fry increased in treatments fed probiotics or immunostimulents. The use of garlic in overwintering feeds could allow hatchery operators to increase their prices for fry and fish farmers to stock production ponds earlier, increasing hatchery revenues by 74% and improving land use efficiency and productivity for the Egyptian aquaculture industry.  相似文献   
68.
Sarcophine-diol (SD) is a semi-synthetic derivative of sarcophine with a significant chemopreventive effect against non-melanoma skin cancer both in vitro and in vivo. Recently, we have studied the effect of SD on melanoma development using the mouse melanoma B16F10 cell line. In this study, our findings show that SD suppresses cell multiplication and diminishes membrane permeability for ethidium bromide (EB), a model marker used to measure cell permeability for Ca2+ ions. SD also decreases protein levels of COX-2, and increases degradation of phospholipases PLA2 and PLCγ1 and diminishes enzymatic activity of the Ca2+-dependent cPLA2. This lower membrane permeability for Ca2+-ions, associated with SD, is most likely due to the diminished content of lysophosphosphatidylcholine (lysoPC) within cell membranes caused by the effect of SD on PLA2. The decrease in diacylglycerol (DAG) and inositol 1,4,5-triphosphate (IP3) due to inhibition of PLCγ1, leads to the downregulation of Ca2+-dependent processes within the cell and also inhibits the formation of tumors. These findings support our previous data suggesting that SD may have significant potential in the treatment of melanoma.  相似文献   
69.
The effect of various dietary starch to proteins ratios (STA/P) on growth performance, oxidative status and liver enzyme activities involved in intermediary metabolism in juvenile Nile tilapia was evaluated. Four isocaloric‐practical diets (12.73 MJ kg?1 digestible energy) with increasing STA/CP ratios were formulated. These were designated D0 (344 g crude protein (CP) and 163.5 g starch (STA) kg?1), D1 (310 g CP and 243 g STA kg?1), D2 (258 g CP and 322 g STA kg?1) and D3 (214 g CP and 401 g STA kg?1). Each diet was fed to triplicate groups of 60 fish (2.7 g) for 45 days. Compared with the control diet (D0), significantly (P < 0.05) depressed growth and feed efficiency were observed only in the groups fed on diet D3. The activities of hepatic enzymes involved in glycolysis and lipogenesis pathways were significantly enhanced in groups fed on diet D3 compared with other diets. A significant (P < 0.05) increase in catalase activity was detected only in groups fed on diet D3. Similarly, a significant (P < 0.05) enhancement in superoxyde dismutase, glutathione S‐transferases and glutathione peroxidise was observed in groups fed on diets D2 and D3 compared with other diets. Results demonstrate the ability of juvenile Nile tilapia to spare protein by dietary carbohydrate.  相似文献   
70.
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