Effect of a probiotic Lactobacillus plantarum TN8 strain on trinitrobenzene sulphonic acid‐induced colitis in rats          下载免费PDF全文

I. Trabelsi  N. Ktari  S. Ben Slima  K. Hamden  R. Ben Salah 《Journal of animal physiology and animal nutrition》2017,101(2):311-319

This study aimed to investigate the potential effects of an oral treatment by a newly isolated probiotic Lactobacillus plantarum TN8 strain on trinitrobenzene sulphonic acid (TNBS)‐induced colitis in Wistar rats. Thus, 18 rats were divided into three groups (n = 6 per group): group 1 (control) – rats not receiving TNBS application; group 2 – rats receiving an intrarectal TNBS infusion (100 mg/kg TNBS dissolved in ethanol); and group 3 – rats treated with intragastrical TN8 strain once per day (for 5 days before TNBS induction). The performance and the effects of the probiotic treatment were evaluated using a series of histological, biophysical and biochemical analyses. The results have shown that the treatment with the L. plantarum TN8 strain improves the body weight and reduces the diarrhoea, colonic mucosal inflammation and colon shortening. TN8‐treated rats showed a significant decrease in the total cholesterol content from 1.86 (for group 2) to 1.32 mmol/l and in triglyceride (TG) content from 2.09 (for group 2) to 1.23 mmol/l. Furthermore, the findings revealed that the high‐density lipoprotein (HDL) cholesterol contents increased from 0.95 to 1.02 mmol/l. The histological studies have confirmed that the architecture of the liver and kidney tissues of the TN8‐treated rats were found to be improved. Overall, the results suggest that the L. plantarum TN8 presents promising perspectives for the development of safe and cost‐effective agents for the prevention or alleviation of several intestinal pathologies.  相似文献   

A note on the persistence of phosfolan on cotton leaves and glass surfaces under field conditions     

Mohammed H. Belal  Hosni S. M. Fahmy  Mounir D. Abdallah  Toson M. Awad 《Pest management science》1978,9(1):63-64

A study of phosfolan deposited from sprays indicated that most of it is degraded within a week in the presence of light and air. It disappears more rapidly on cotton leaves than on glass because much of it migrates into the leaf interior within 7 h of application. Degradation within the leaf proceeds more slowly than on the leaf surface or on glass.  相似文献   

Induction of non-specific suppression in chicks by specific combination of maternal antibody and related antigen     

Mohamed Fahmy ABOU ELAZAB  Hiroyuki HORIUCHI  Shuichi FURUSAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1363-1369

Specific immune suppression in newly hatched chicks induced by specific maternal antibodies has been reported. Laying hens were immunized with dinitrophenyl-keyhole limpet hemocyanin (DNP-KLH). Purified maternal anti-DNP and non-specific immunoglobulin (Ig) Y antibodies were transferred by yolk sac inoculation to newly hatched chicks, and then, they were immunized with an optimum immunogenic dose of DNP-KLH at 1 and 4 weeks of age. Concentrations of anti-DNP antibodies in serum samples of these chicks were measured by using Enzyme-linked immunosorbent assay (ELISA). Proportions of T-cell subsets in peripheral blood of these chicks were also measured by flow cytometric analysis at 5 weeks of age (one week after the second immunization). Suppression of anti-DNP antibody response and down-regulation of CD3⁺CD4⁺ cells were observed in the chicks received high dose of maternal anti-DNP antibodies and immunized with DNP-KLH. On the other hand, normal anti-DNP antibody response and normal proportion of CD3⁺CD4⁺ cells were observed in the chicks received high dose of non-specific IgY antibodies and immunized with DNP-KLH. Furthermore, when chicks received high dose of maternal anti-DNP antibodies and immunized with DNP-KLH at 1 and 4 weeks of age and then with rabbit serum albumin (RSA) at 5 and 8 weeks of age, their primary anti-RSA response was also significantly suppressed. We indicate here that specific maternal antibodies can affect both B and T cell responses and induce non-specific suppression against different antigens. However, this non-specific suppression does not continue for a long time.  相似文献   

Fasciola gigantica excretory-secretory products for immunodiagnosis and prevention of sheep fasciolosis     

El Ridi R  Salah M  Wagih A  William H  Tallima H  El Shafie MH  Abdel Khalek T  El Amir A  Abo Ammou FF  Motawi H 《Veterinary parasitology》2007,149(3-4):219-228

Excretory-secretory products (ESP) products of ex vivo Fasciola gigantica adult worms were used for immunodiagnosis of sheep experimental infection with F. gigantica and natural infection with Fasciola spp. by enzyme-linked immunosorbent assay (ELISA) and Western blotting. Specific IgG antibody binding to native or denatured ESP was detected as early as 2 weeks after experimental sheep infection with 100 or 200 metacercariae. No specific IgG antibody binding was displayed by sera obtained from 192 sheep considered to be Fasciola- and other parasite-free by microscopic examination of bile and feces. Additionally, sera from 200 apparently Fasciola-free sheep, yet infected with other parasites, were all negative. The data, thus, indicated that ESP-based ELISA reached nearly 100% sensitivity and specificity in immunodiagnosis of sheep fasciolosis. As expected, the ESP molecules were immunogenic in sheep eliciting interleukin-12p40 mRNA response and considerable amounts of antibodies, which were able to bind to the surface of newly excysted juvenile worms as judged by membrane indirect immunofluorescence, and mediate their attrition via antibody-dependent cell-mediated cytotoxicity. The ESP-induced cellular and humoral immune responses were associated with a modest reduction in worm count, yet with a highly significant (P<0.0001) decrease in size of recovered worms, thus suggesting that ESP immunization might be a safe and cost-effective strategy for reducing transmission of the infection.  相似文献   

Antimicrobial resistance pattern of Salmonella serotypes isolated from apparently healthy slaughtered camels (Camelus dromedarius) in eastern Ethiopia     

Molla B  Salah W  Alemayehu D  Mohammed A 《Berliner und Münchener tier?rztliche Wochenschrift》2004,117(1-2):39-45

A total of 714 samples consisting of faeces, mesenteric lymph nodes, liver, spleen, abdominal and diaphragmatic muscles (each 119) were collected from November 2001 to April 2002 from apparently healthy slaughtered camels (Camelus dromedarius) in eastern Ethiopia. One hundred sixteen (16.2%) Salmonella strains belonging to 16 different serovars were isolated. All Salmonella strains isolated were examined for antimicrobial resistance to 17 selected antimicrobials. The minimum inhibitory concentration (MIC) values were determined by the microdilution broth test. Fifty-two (44.8%) of the Salmonella isolates were resistant to one or more antimicrobials. Thirty-nine of the 52 (75%) resistant Salmonella serovars exhibited multiple resistance to up to eight different antimicrobials. Among the serovars tested, S. Typhimurium, S. Heidelberg, S. Braenderup and S. Hadar displayed multiple resistance mainly to streptomycin (35.3%), spectinomycin (28.4%), sulfamethoxazole (25.0%), ampicillin (24.1%), trimethoprim (22.4%), trimethoprim/sulfamethoxazole (18.9%), tetracycline (12.9%) and colistin (11.2%). All Salmonella strains tested were susceptible to ciprofloxacin, nalidixic acid, gentamicin, kanamycin and neomycin. The present study showed the importance of camels as a potential source of single and multiple resistant Salmonella strains to different antimicrobials that are also used in the public health sector for the treatment of different bacterial diseases in Ethiopia.  相似文献   

Aryl N-hydroxy- and N-methoxy-N-methylcarbamates as potent reversible inhibitors of acetylcholinesterase     

Y.C. Chiu  M.A.H. Fahmy  T.R. Fukuto 《Pesticide biochemistry and physiology》1973,3(1):1-6

Several aryl N-hydroxy- and N-methoxy-N-methylcarbamates were examined as inhibitors of bovine erythrocyte acetylcholinesterase (AChE). These carbamate derivatives were generally strong inhibitors of AChE, but, unlike the typical N-methyl- and N,N-dimethylcarbamates which are carbamylating agents, they proved to be reversible, competitive inhibitors of the enzyme. The values for the dissociation constant (K_a) for the enzyme-inhibitor complex to enzyme and inhibitor were in the range of 2 × 10^?5?1 × 10^?7M.  相似文献   

Effects of glucagon‐like peptides 1 and 2 and epidermal growth factor on the epithelial barrier of the rumen of adult sheep     

Gabriele Greco  Salah Amasheh  Zanming Shen  Zhongyan Lu  Jrg R. Aschenbach 《Journal of animal physiology and animal nutrition》2019,103(6):1727-1738

Epidermal growth factor (EGF) and glucagon‐like peptides (GLP) modulate the tight junctions (TJ) of the intestinal epithelial barrier (EB) of monogastric animals. This work tried to elucidate whether GLP‐1, GLP‐2 and EGF can affect the EB of the rumen. Ovine ruminal epithelia were incubated in Ussing chambers for 7 hr with 25 or 250 nM of either GLP‐1 or GLP‐2 on the serosal side, with 2.5 nM of EGF on the serosal side or with 0.25 or 2.5 nM EGF on the mucosal side. No treatment affected tissue conductance. Short‐circuit current (I_sc) was affected by time and treatment and their interactions. Only 250 nM of either GLP‐1 or GLP‐2 decreased I_sc in certain periods compared with 25 nM GLP‐1 or 0.25 nM mucosally applied EGF; however, not when compared to control epithelia. Fluorescein flux rates (J_fluor) of ruminal epithelia were affected by treatment, time and time × treatment interaction. The time × treatment interaction was based on an increase in J_fluor between the first and last hour in epithelia incubated with 25 nM GLP‐1 or GLP‐2 and in epithelia incubated with EGF. After 7 hr incubation, claudin‐7 mRNA expression was downregulated in all treatments. Claudin‐1 mRNA was upregulated after incubation with 2.5 nM EGF on the serosal side, claudin‐4 mRNA was downregulated by 2.5 nM EGF on the mucosal side, and occludin mRNA was increased after incubation with 250 nM GLP‐2. The protein abundance of all tested TJ proteins was not influenced by treatment. We conclude that GLP‐1, GLP‐2, and EGF have no obvious acute effects on the EB of ruminal epithelia under simulated physiological conditions ex vivo. However, by decreasing the mRNA expression of claudin‐7 and partly affecting other TJ proteins, they may modulate EB in the longer term or under certain conditions.  相似文献   

A plant growth promoting rhizobacterium and temperature effects on performance of 18 clones of potato   总被引：1，自引：0，他引：1  

Salah Bensalim  Jerzy Nowak  Samuel K. Asiedu 《American Journal of Potato Research》1998,75(3):145-152

A survey of genotypic responses to beneficial bacterium (Pseudomonas sp. strain PsJN) was conductedin vitro andex vitro, under two temperature conditions, using eighteen clones of potato of different heat stress tolerance: temperate adapted cultivars Kennebec and Russet Burbank; heat tolerant DTO-2, DTO-28, DTO-33, LT-1, LT-2, LT-5, LT-6, LT-7, LT-8, LT-9, Y84-02, NDD277-2, Désirée, and Maine-47; and heat sensitive abscissic acid (ABA)-deficient mutants 11401-01 and 9120-05. Nodal explants taken from 6-week-old bacterized and non-bacterized control plantlets were culturedin vitro on a hormone-free potato nodal cutting medium, and placed at either 20/15 C or 33/25 C day/night temperature, 12h photoperiod and 250 µE m^?2 s^?1 mixture of fluorescent and incandescent light, for six weeks. The tuberization response was studiedex vitro after two weeks acclimation of 2-week old plantlets at 33/25C. The acclimated plantlets were transplanted to 3L plastic nursery pots containing peat-based Pro-Mix growing medium and placed in growth chambers at either 20/15 or 33/25 C day/night temperature, 12 h photoperiod, 475 μE m^?2 s^?1 light and ≈80% RH, for 12 weeks. Compared to the non-bacterized controls, bacterization significantly increased stem length of 12, shoot biomass of 9, and root biomass of 2 clones at 20/15C; and stem length of 14, shoot biomass of 15, and root biomass of 13 clones at 33/25C. High temperature increased length of internodes and had either no effect or slightly decreased node number. Temperature increase had the most dramatic effect on root development. An average shoot to root ratio decreased from 3.7 at 20/15 C to 1.7 at 33/25 C for non-bacterized plantlets and, respectively, from 4.3 to 1.5 for bacterized. The beneficial effect of bacterization on root biomass was the most pronounced in LT-1 and Maine-47 at 20/15 C and LT-8, Maine 47, DTO-2, Kennebec, NDD277-2 and 11401-01 at 33/25C. The temperature elevation did not significantly affect root biomass of LT-6, DTO-28 and Désirée. Temperature stress caused severe reduction in tuber number and tuber fresh weight. ABA-deficient mutants did not produce any tubers and LT-8, LT-9, Y84-027 and DTO-28 tuberized very poorly at 33/25C. DTO-33, Désirée, LT-1 and Kennebec gave the highest number of tubers per pot and Kennebec, LT-1, Désirée and LT-7 the highest yields at this temperature. There was no significant effect of bacterization on tuberization at 20/15 C but at 33/25 C bacterization significantly enhanced tuber number and weight in LT-7 and reduced tuber weight in DTO-2. Although there was no clear link between thein vitro response of particular clones to bacterization and their heat stress tolerance, improvement ofex vitro performance of heat tolerant LT-7 indicates that rhizosphere bacteria may play a role in clonal adaptation of potato to heat stress.  相似文献   

Optimal coupling combinations between irrigation frequency and rate for drip-irrigated maize grown on sandy soil   总被引：1，自引：0，他引：1  

Salah E. El-Hendawy  Urs Schmidhalter 《Agricultural Water Management》2010,97(3):439-5300

This study was conducted over 2 years (2007 and 2008) to establish the optimal combinations between irrigation frequency and rate for drip-irrigated maize using water production functions and water use-yield relationships. A field experiment was conducted using a randomized complete block split plot design with four irrigation frequencies (F₁, F₂, F₃ and F₄, irrigation events once every 1, 2, 3 or 4 days, respectively) and three drip irrigation rates (I₁: 1.00, I₂: 0.80, and I₃: 0.60 of the estimated evapotranspiration, ET) as the main and split plots, respectively. Our results show that yield variables and water use efficiencies (WUEs) increased with increasing irrigation frequency and rate, with non-significant differences between F₁ and F₂ in yield variables and between I₁ and I₂ in WUEs. Moreover, the combination between various irrigation frequencies and rates had an important effect on yield variables and WUEs, with the highest values being found for F₁I₂ and F₂I₁ and the lowest for F₃I₃ and F₄I₃. The F₁I₃ treatment had grain yield and yield components values similar to those obtained for the F₃I₂ and F₄I₁ treatments and WUEs values similar to those obtained for the F₂I₁ and F₂I₂ treatments. Seasonal yield response factors (k_y) were 1.81 and 1.86 in 2007 and 2008, respectively. Production functions of yield versus seasonal crop ET were linear for all combinations of irrigation frequency and rate and for all irrigation frequency treatments with the exception of the F₁ treatment, which instead showed a second order relationship. The relationship between WUE and grain yield was best represented by a power equation. In conclusion, we identified the optimal coupling combinations between irrigation frequency and water application rate to achieve the maximum yield and WUEs under either sufficient (F₂I₁) or limited irrigation (F₁I₃) water supplies.  相似文献   

Physico-chemical characteristics and total quality of date palm varieties grown in the southern of Tunisia     

Mrabet A  Ferchichi A  Chaira N  Mohamed BS  Baaziz Z  Penny TM 《Pakistan journal of biological sciences: PJBS》2008,11(7):1003-1008

The date palm, Phoenix dactylifera, is a palm extensively cultivated for its edible fruit. The chemical composition and the water content of ten Tunisian date varieties were determined. For all analysis, the Deglet Nour variety was taken as reference. Compositional analysis showed that the littoral varieties were very rich in reducing sugars (26 to 51%) than Deglet Nour which was rich in sucrose (54%). The relative results of the moisture content showed that the littoral varieties were classified as soft dates. The vitamin C analysis showed that the littoral varieties were very rich in this compound (24 to 46 mg/100 g) than Deglet Nour (1.12 mg/100 g). The mineral analysis showed that the littoral dates were relatively rich in potassium (283 to 733 mg/100 g) and presented a weak content in sodium (0.06 to 0.09 mg/100 g).  相似文献