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31.
32.
SC Totton AM Farrar W Wilkins O Bucher LA Waddell BJ Wilhelm SA McEwen A Rajić 《Preventive veterinary medicine》2012,106(3-4):197-213
Eating inappropriately prepared poultry meat is a major cause of foodborne salmonellosis. Our objectives were to determine the efficacy of feed and water additives (other than competitive exclusion and antimicrobials) on reducing Salmonella prevalence or concentration in broiler chickens using systematic review-meta-analysis and to explore sources of heterogeneity found in the meta-analysis through meta-regression. Six electronic databases were searched (Current Contents (1999-2009), Agricola (1924-2009), MEDLINE (1860-2009), Scopus (1960-2009), Centre for Agricultural Bioscience (CAB) (1913-2009), and CAB Global Health (1971-2009)), five topic experts were contacted, and the bibliographies of review articles and a topic-relevant textbook were manually searched to identify all relevant research. Study inclusion criteria comprised: English-language primary research investigating the effects of feed and water additives on the Salmonella prevalence or concentration in broiler chickens. Data extraction and study methodological assessment were conducted by two reviewers independently using pretested forms. Seventy challenge studies (n=910 unique treatment-control comparisons), seven controlled studies (n=154), and one quasi-experiment (n=1) met the inclusion criteria. Compared to an assumed control group prevalence of 44 of 1000 broilers, random-effects meta-analysis indicated that the Salmonella cecal colonization in groups with prebiotics (fructooligosaccharide, lactose, whey, dried milk, lactulose, lactosucrose, sucrose, maltose, mannanoligosaccharide) added to feed or water was 15 out of 1000 broilers; with lactose added to feed or water it was 10 out of 1000 broilers; with experimental chlorate product (ECP) added to feed or water it was 21 out of 1000. For ECP the concentration of Salmonella in the ceca was decreased by 0.61log(10)cfu/g in the treated group compared to the control group. Significant heterogeneity (Cochran's Q-statistic p≤0.10) was observed among studies examining all organic acids (controlled or challenge experiments), butyric acid, formic acid, a formic/propionic acid mixture, fermented liquid feed, and D-mannose. Meta-regressions were conducted to examine the source of heterogeneity among studies. For prevalence outcomes, 36% and 60% of the total variance was within and between studies, respectively. For concentration outcomes, 39% and 33% of the total variance was within and between studies, respectively. Inadequate blinding and randomization was common, and no studies undergoing meta-analysis or meta-regression were conducted on a commercial farm. The strength of evidence of the effect of these additives was very low. Studies conducted under commercial conditions are needed to understand the potential benefit of these interventions for the poultry industry and to improve the strength of evidence of the effectiveness of these additives. 相似文献
33.
The results of a 2 year study of the effect of trickle and furrow irrigation methods on hop production in a desert region in northwest United States are presented. Also discussed are the implications to hop production in areas with higher rainfall.Trickle, furrow, and cutback furrow irrigation systems and their associated methods of fertilization were evaluated in a commercial hop yard. Leaf diffusive resistance, leaf water potential, alpha and beta acid content, and yield were measured. Also monitored was the efficiency of irrigation water use and the intregration of these irrigation methods with commercial crop production practices. Trickle irrigation with fertilizer injection provided several crop management advantages but required changes in cultivation practices. In the 2nd year of study, the highest yield and quality of hop was obtained from trickle irrigated plots which received the least amounts of water, fertilizer and labor inputs. 相似文献
34.
R B Young D M Moriarity C E McGee W R Farrar H E Richter 《Journal of animal science》1990,68(4):1158-1169
Primary muscle cell cultures were prepared from the leg muscle of 12-d broiler chicken embryos. The partitioning agent cimaterol (10(-6) to 10(-10) M) was added on d 1 and each day thereafter, and cells were studied after 7 d in culture. Cimaterol had no effect at any level either on the percentage of nuclei within multinucleated myotubes or on the total number of nuclei within myotubes. At 10(-7) M cimaterol, the quantity of the myofibrillar protein fraction was increased by 25.1 +/- 8.0% (P less than .05) and the quantity of myosin heavy chain was increased by 30.9 +/- 4.5% (P less than .05). To understand the basis for the increase in myofibrillar protein, the incorporation rate of [3H]Leu was measured in pulse labeling experiments. The apparent synthesis rate of the soluble protein fraction and the crude myofibrillar fraction was not significantly increased by cimaterol; however, cimaterol levels greater than 10(-8) M caused a 10 to 12% increase (P less than .05) in the incorporation rate of [3H]Leu into myosin heavy chain. The effect of cimaterol on release of [3H]Leu from prelabeled protein also was assessed in pulse-chase experiments; the apparent rate of protein degradation was inhibited by 10 to 15% (P less than .05) at the higher levels of cimaterol. Dot blot analysis indicated that the quantity of myosin heavy chain mRNA was elevated in cimaterol-treated cultures. Thus, the increased quantity of myofibrillar proteins in embryonic broiler muscle cell cultures is the combined result of a stimulation in the rate of protein synthesis and an inhibition in the rate of protein degradation. 相似文献
35.
J E Wagner P L Farrar 《Veterinary Clinics of North America: Small Animal Practice》1987,17(5):1061-1087
Biology, physiology, husbandry, and commonly occurring diseases of small rodent pets, including the Syrian hamster, Mongolian gerbil, rat, and mouse, are presented. 相似文献
36.
Free amino acids (AA's) represent a significant source of available N for some plants and soil microorganisms. It can be expected, however, that significant competition will exist between plants and microorganisms for this organic N resource. Our study indicated that microbial capture and utilization of glycine was very rapid at a range of soil solution concentrations (0.1 μM to 10 mM) indicating that significant competition will exist between roots and soil microorganisms. Plant capture of free AA's was maximal at high soil solution concentrations where microbial utilization was slowest. Our results suggest that plant capture of soil dissolved organic N may primarily occur in organic rich patches in soil where concentrations of free AA's are high. 相似文献
37.
Elizabeth Boddy Paula Roberts Paul W. Hill John Farrar David L. Jones 《Soil biology & biochemistry》2008,40(7):1557-1566
Polar ecosystems are currently experiencing some of the fastest rates of climate warming. An increase in soil temperature in High Arctic regions may stimulate soil permafrost melting and microbial activity, thereby accelerating losses of greenhouse gases. It is therefore important to understand the factors regulating the rates of C turnover in polar soils. Consequently, our aims were to: (1) assess the concentration of low molecular weight (MW) dissolved organic carbon (DOC) in soil, (2) to investigate the temperature-dependent turnover of specific low MW compounds, and (3) to analyse the influence of substrate concentration on C cycling. Microbial mineralisation of labile low MW DOC in two High Arctic tundra soils was investigated using soil solutions spiked with either 14C-labelled glucose or amino acids. Spiked solutions were added to the top- and sub-soil from two ecosystem types (lichen and Carex dominated tundra), maintained at three temperatures (4–20 °C), and their microbial mineralisation kinetics monitored. 14CO2 evolution from the tundra soils in response to 14C-glucose and -amino acid addition could best be described by a double first order exponential kinetic equation with rate constants k1 and k2. Both forms of DOC had a short half-life (t1/2) in the pool of microbial respiratory substrate (t1/2 = 1.07 ± 0.10 h for glucose and 1.63 ± 0.14 h for amino acids; exponential coefficient k1 = 0.93 ± 0.07 and 0.64 ± 0.06 h?1 respectively) whilst the second phase of mineralisation, assumed to be C that had entered the microbial biomass, was much slower (average k2 = 1.30 × 10?3 ± 0.49 × 10?4 h?1). Temperature had little effect on the rate of mineralisation of 14C used directly as respiratory substrate. In contrast, the turnover rate of the 14C immobilized in the microbial biomass prior to mineralisation was temperature sensitive (k2 values of 0.99 × 10?3 h?1 and 1.66 × 10?3 h?1 at 4 and 20 °C respectively). Concentration-dependent glucose and amino acid mineralisation kinetics of glucose and amino acids (0–10 mM) were best described using Michaelis–Menten kinetics; there was a low affinity for both C substrates by the microbial community (Km = 4.07 ± 0.41 mM, Vmax = 0.027 ± 0.005 mmol kg?1 h?1). In conclusion, our results suggest that in these C limiting environments the flux of labile, low MW DOC through the soil solution is extremely rapid and relatively insensitive to temperature. In contrast, the turnover of C incorporated into higher molecular weight microbial C pools appears to show greater temperature sensitivity. 相似文献
38.
Roumagnac P Weill FX Dolecek C Baker S Brisse S Chinh NT Le TA Acosta CJ Farrar J Dougan G Achtman M 《Science (New York, N.Y.)》2006,314(5803):1301-1304
For microbial pathogens, phylogeographic differentiation seems to be relatively common. However, the neutral population structure of Salmonella enterica serovar Typhi reflects the continued existence of ubiquitous haplotypes over millennia. In contrast, clinical use of fluoroquinolones has yielded at least 15 independent gyrA mutations within a decade and stimulated clonal expansion of haplotype H58 in Asia and Africa. Yet, antibiotic-sensitive strains and haplotypes other than H58 still persist despite selection for antibiotic resistance. Neutral evolution in Typhi appears to reflect the asymptomatic carrier state, and adaptive evolution depends on the rapid transmission of phenotypic changes through acute infections. 相似文献