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31.
Fatemeh Davami Farnaz Eghbalpour Leila Nematollahi Farzaneh Barkhordari Fereidoun Mahboudi 《Iranian Biomedical Journal》2015,19(4):194-205
Background:
The optimization of bioprocess conditions towards improved growth profile and productivity yield is considered of great importance in biopharmaceutical manufacturing. Peptones as efficient sources of nutrients have been studied for their effect on media development; however, their role on metabolic pathway is not well understood.Methods:
In the present study, the effect of different concentration of peptones on a recombinant Chinese hamster ovary (CHO) cell line grown in three serum-free suspension cultures was determined. Six peptones of different origins and available amino acid profiles were investigated regarding their impact on cell growth, productivity, and metabolic pathways changes.Results:
In optimized feeding strategies, increases of 136% and 159% in volumetric productivity (for a low-nutrient culture media) and 55% (for a high-nutrient culture media) were achieved. Furthermore, particular sources of peptones with specific amino acid profile developed preferential results for each different culture medium. Two peptones, SoyA2SC and SoyE-110, were the only hydrolysates that showed production improvement in all three media. Casein Peptone plus Tryptone N1 and SoyA3SC showed different improved results based on their implemented concentration for each individual basal medium.Conclusion:
The amino acid profile of peptones may provide clues to identify the most effective feeding strategies for recombinant CHO cells.Key Words: CHO cells, Culture media, Peptones, Recombinant proteins 相似文献32.
Khavari-Nejad RA Najafi F Khavari-Nejad S 《Pakistan journal of biological sciences: PJBS》2008,11(10):1390-1393
The comparative responses of certain biochemical and physiological characteristics to salinity were studied in 4 cultivars of sugar beet (Beta vulgaris L.) plants. Eight weeks old plants were treated with NaCl at 0, 25 and 50 mM in nutrient solutions. Plants were grown under controlled environment and harvested after 3 weeks for measurements of biochemical and physiological parameters. Results showed that in 25 mM NaCl for cultivars of ET5 and C3-3, soluble sugars in leaves, photosynthetic rate and growth parameters were significantly increased as compared to those of other cultivars. In 50 mM NaCl photosynthetic rate and soluble sugars were significantly increased only in ET5 cultivar as compared with those of others. Results indicated that in 25 mM NaCl, ET5 cultivar showed high growth responses and tolerated to 50 mM NaCl. 相似文献
33.
Farzaneh Noori Naser Agh Fatemeh Jafari Reza Jalili Enric Gisbert Mansour Torfi Mozanzadeh 《Aquaculture Nutrition》2019,25(5):1050-1062
The influence of dietary fish oil (FO) substitution with blends of vegetal oils (VO: canola, linseed, olive, sunflower, corn and coconut oils) in plant protein‐rich diets on reproductive performance and fatty acid dynamics of embryos was evaluated in female rainbow trout (Oncorhynchus mykiss) brooders (1.8 ± 0.1 kg). Four diets were formulated in which 20% (FO80/VO20), 50% (FO50/VO50), 75% (FO25/VO75) and 100% (VO100) of FO were replaced by mixture of VO. The above‐mentioned diets were administered for a short period prior to spawning (3 months). Fish fed the VO100 had the lowest fertilization rate (81.3 ± 2.3), whereas brooders fed the FO80/VO20 diet had the lowest survival rates at eyed embryo stage (83.7 ± 1.6%) and hatching rate (79.9 ± 3.1%). The fatty acid dynamics of embryos were not only affected by embryonic developmental stages, but also they were influenced by the dietary fatty acid profile. Our study confirmed that using blends of different VO sources and FM residual fat in the FO25/VO75 for the short‐term period prior to spawning seemed to be a good strategy in terms of successful reproduction for sparing high levels of FM and FO in diet of O. mykiss brooders. 相似文献
34.
Naser Agh Mansour Torfi Mozanzadeh Fatemeh Jafari Farzaneh Noori Reza Jalili 《Aquaculture Research》2020,51(3):918-931
A 90‐day feeding trial was carried out to examine the influence of fish oil (FO) substitution with blends of vegetable oils (VOs) on reproductive efficiency of female brooders and fluctuation in fatty acid (FA) profile of embryos in Oncorhynchus mykiss. A basal diet was formulated in which 20% (80FO/20VO), 50% (50FO/50VO), 75% (25FO/75VO) and 100% (100VO) of FO were replaced by mixture of VO. Reproductive performance of brooders was not affected by drastic alternations in FA profile of diets. The level of saturated and monounsaturated FAs (MUFAs) significantly increased, whereas the levels of long‐chain polyunsaturated FAs (LC‐PUFAs), mainly docosahexaenoic acid, profoundly decreased during embryogenesis. The concentrations of MUFA and n?6 PUFA increased in the eggs with increasing the incorporation of VO mixture in diets; however, the concentration of LC‐PUFA and n?3/n?6 PUFA ratio decreased. Haematological parameters and humoral immune responses including total immunoglobulin content, lysozyme and alternative complement pathway activities in brooders fed with the experimental diets did not statically different. In summary, incorporating mixture of various VO sources especially linseed and sunflower oils as good sources of α‐linolenic and linoleic acids, respectively, along with low levels of residual fat from fish meal in diet suggested a good strategy for providing the appropriate essential FA requirements of O. mykiss brooders for their successful reproduction. 相似文献
35.
Reza Moazzami Hasan Mirzahoseini Leila Nematollahi Farzaneh Barkhordari Mozhgan Raigani Fatemeh Hajari Taheri Fereidoun Mahboudi Fatemeh Davami 《Iranian Biomedical Journal》2021,25(4):275
Background:Bispecific antibodies represent an important class of mAbs, with great therapeutic potentials due to their ability to target simultaneously two distinct epitopes. The generation of functional bispecific antibodies with the highest possible yields is particularly critical for the production of these compounds on industrial scales. Anti- CD3 × CD19 bsAb is a bispecific T-cell engager (BiTE) currently used for treating ALL. Herein, we have tried to optimize the expression level of this antibody in mammalian hosts. Methods:WPRE sequence was incorporated at the 3’ end of the expression cassette. This modification resulted in a notable about two-fold increase in the expression of the bsAb in the Expi293 cell line. Results & Conclusion:Follow-up flow cytometry analysis demonstrated the binding properties of the produced antibody at acceptable levels, and in vitro bioactivity assays showed that this product is potent enough for targeting and destroying CD19-positive cells. Our findings show that WPRE enhances the expression of this type of bispecific mAbs in HEK-293 family cell lines. This approach can be used in biopharma industry for the mass production of anti-CD3 × CD19 bispecific antibody. Key Words: Acute lymphoblastic leukemia, Bispecific antibodies, Monoclonal antibody 相似文献
36.
BACKGROUND: Linear alkylbenzene sulfonate (LABS) is an anionic surfactant widely used all over the world. They will eventually end-up and accumulate in household or industrial sewage. Due to their high foaming capabilities which can cause numerous problems in sewage treatment facilities as well as direct toxic effects on many different organisms in ecosystem; they are generally considered as serious pollutants. Many reports have indicated that common bacteria can readily degrade LABS. METHODS: In this survey, two different bacteria were isolated from Tehran municipal active sludge that showed the ability to degrade LABS rapidly and actively upon using it as their sole source of carbon. Biochemical tests as well as 16S rRNA gene sequencing performed. RESULTS: Results have indicated the two isolates to be Acinetobacter johnsoni and Pseudomonas beteli. After experiments to optimize the pH and temperature for growth of the two bacterial isolates, the extent of LABS, utilization was evaluated by HPLC method. The Pseudomonas beteli and Acinetobacter johnsoni isolates were able to degrade 96.4% and 97.2% of the original LABS levels after 10 days of growth, respectively. Mixed culture of the two isolates did not significantly increase LABS utilization (97.6%). CONCLUSION: Our study showed the ability of two isolated steains to rapidly biodegrade LABS under aerobic conditions. 相似文献
37.
BACKGROUND: Evidence supports the involvement of nitric oxide (NO) in a variety of male reproductive processes such as spermatogenesis, spermiogenesis, sperm motion, sperm metabolism and sperm capacitation. However, low concentration of NO is essential in biology and physiology of spermatozoa, but high amounts of NO is toxic and has negative effects on sperm functions. On the other hand, it is established that high amounts of NO have detrimental effects on DNA. The integrity of sperm DNA is an important factor in successful fertility and embryo development. It is hypothesized that supra physiological concentrations of NO in seminal plasma cause sperm DNA damage. The aim of this study was to determine sperm DNA damage by comet assay and its correlation with NO level in seminal plasma of fertile and infertile men. METHODS: Semen samples were collected from 45 patients and 70 healthy donors. The stable metabolites of NO (nitrite and nitrate) in seminal plasma were measured by Griess assay and DNA damage was determined using single cell gel electrophoresis (comet) assay method. RESULTS: The NO concentration in the seminal plasma of infertile males was significantly higher than fertile males (5.74+/-1.01 microM/L vs. 3.88+/-0.53 microM/L). There was a significant positive correlation between the NO concentration and sperm DNA comet value in infertile males (P<0.01, R=0.598). CONCLUSION: These results indicate that the overproduction of NO in genital tract of infertile males has a potential pathogenetic role in the reduction of sperm DNA integrity. 相似文献
38.
Efficacy of conventional and extended intra-mammary treatment of persistent sub-clinical mastitis with cefquinome in lactating dairy cows 总被引：1，自引：1，他引：0
Kasravi R Bolourchi M Farzaneh N Seifi HA Barin A Hovareshti P Gharagozlou F 《Tropical animal health and production》2011,43(6):1203-1210
The objectives of the present study were to evaluate the efficacy of intra-mammary-administered cefquinome for the treatment
of sub-clinical mastitis in lactating dairy cows and to determine if extended therapy would enhance treatment efficacy. Seventy-three
Holstein dairy cows from a single farm with 150 infected quarters were enrolled in the study. Infected cows were allocated
randomly to one of three treatment regimens: (1) conventional (standard) regimen: 75 mg of cefquinome administered three times
at 16-h intervals (25 infected cows, 52 intra-mammary infections (IMI)), (2) extended regimen: 75 mg of cefquinome administered
six times at 16-h intervals (26 infected cows, 58 IMI) and (3) negative untreated control group (22 cows, 40 IMI). Most IMI
were caused by coagulase-negative staphylococci, streptococci other than Streptococcus agalactiae and coliforms. The overall bacteriological cure (BC) rates for sub-clinical IMI were 84.61%, 91.37% and 20% for the conventional,
extended and the control groups, respectively, indicating a higher BC rate for the treated groups than the control group (P < 0.001). Significant differences in somatic cell count (SCC) were detected between the treated versus the control group
(P < 0.001). No differences, concerning the BC rate or SCC, were observed between the extended and the conventional groups.
Although fat and protein percentages increased in the treated groups, there were no significant differences in post-treatment
milk production between the groups. Results of this study indicate that cefquinome therapy was effective in reducing SCC and
eliminating sub-clinical IMI in lactating dairy cows, but extended therapy did not enhance treatment efficacy. 相似文献
39.
In this paper, poly(amido primary-secondary amine) (PAPSA) as a high capacity polymeric adsorbent was synthesized. Dye removal ability of PAPSA from single and binary systems was investigated. The functional groups of PAPSA were studied using Fourier transform infrared (FTIR). Acid Blue 92 (AB92), Direct Red 23 (DR23), and Direct Red 81 (DR81) were used as model compounds. The kinetic and isotherm of dye adsorption were studied. The effect of operational parameter such as adsorbent dosage, dye concentration, and pH on dye removal was evaluated. It was found that adsorption of dyes onto PAPSA showed Langmuir isotherm. The maximum dye adsorption capacity (Q 0) of PAPSA was 10000 mg/g, 12500 mg/g, and 10000 mg/g for AB92, DR23, and DR81, respectively. Adsorption kinetic of dyes followed pseudo-second order kinetics. Dye desorption tests showed that the dye release of 85 % for AB92, 91 % for DR23 and 89 % for DR81 were achieved in aqueous solution at pH 12. The results showed that the PAPSA as a polymeric adsorbent with high dye removal ability might be a suitable alternative to remove dyes from colored wastewater. 相似文献
40.
Somayeh Bohlouli Arezou Rabzia Ehsan Sadeghi Farzaneh Chobsaz Mozafar Khazaei 《Iranian Biomedical Journal》2016,20(1):12-17