排序方式： 共有114条查询结果，搜索用时 15 毫秒
51.
Alonso Pardo-Vargas Ingrid de Barcelos Oliveira Paulo Roberto Soares Stephens Claudio Cesar Cirne-Santos Izabel Christina Nunes de Palmer Paix?o Freddy Alejandro Ramos Carlos Jiménez Jaime Rodríguez Jackson Antonio Lamounier Camargos Resende Valeria Laneuville Teixeira Leonardo Castellanos 《Marine drugs》2014,12(7):4247-4259
52.
Ceelen LM Haesebrouck F Favoreel H Ducatelle R Decostere A 《Veterinary microbiology》2006,113(1-2):45-53
Helicobacter pullorum has been associated with diarrhoea, gastroenteritis and liver disease in humans and with hepatitis and enteritis in poultry. The purpose of the present study was to examine whether cytolethal distending toxin was present among 10 poultry and three human H. pullorum isolates and whether a different level of cytolethal distending toxin activity was noted. A PCR assay was performed to detect the cdtB gene. In addition, epithelial Hep-2 cells inoculated with sonicate from all strains were observed microscopically and DNA analysis of these cells was done by flow cytometry. All H. pullorum isolates harboured the cdtB gene, but functional cytolethal distending toxin activity was only demonstrated in the human H. pullorum strain CCUG 33839. A significant number of cells treated with sonicate from this strain were enlarged. The nuclei were distended proportionally. Giant cells and multinucleated cells were observed as well. In addition, stress fibers accumulated. DNA analysis by flow cytometry revealed 31.0% of these cells at the S/G2 stage of the cell cycle. The tested poultry and human H. pullorum isolates all possess the cdtB gene, but under the circumstances adopted in this study only the human strain CCUG 33839 seems to show biological activity typically for CDT in vitro. 相似文献
53.
Gholamiandehkordi Ahmadreza Eeckhaut Venessa Lanckriet Anouk Timbermont Leen Bjerrum Lotte Ducatelle Richard Haesebrouck Freddy Van Immerseel Filip 《Veterinary research communications》2009,33(8):1031-1037
The antimicrobial susceptibility of Clostridium perfringens strains isolated from Belgian broilers between May and September 2007 was investigated. All 39 tested isolates were sensitive
to enrofloxacin, erythromycin, tylosin, florfenicol and bacitracin. Twenty-six (66%) and 24 (61%) out of the 39 tested isolates
showed acquired resistance to tetracycline and lincomycin, respectively. The C. perfringens isolates were also screened by PCR for the presence of the resistance genes tet(K), tet(L), tet(M), tetB(P), tet(O), tet(W), lnu(A) and lnu(B). In 22/26 tetracycline resistant strains and 7/24 lincomycin resistant strains, resistance could be attributed to one
or more of these genes. An extended frequency distribution range of MICs was seen for ampicillin. These data are consistent
with data derived from studies carried out in 1980 and in 2004, indicating that no changes in antimicrobial resistance patterns
have taken place during time in C. perfringens isolates from broilers in Belgium. 相似文献
54.
Geny L Saucier C Bracco S Daviaud F Glories Y 《Journal of agricultural and food chemistry》2003,51(27):8051-8054
Cell walls were isolated from seeds of grape berries (Vitis vinifera L.), and proanthocyanidin composition was determined over the course of ripening for different levels of vine water status. During the ripening period the tannins from the cell walls were always more polymerized than those from the inner part of the cell. At maturity this difference becomes more significant compared to véraison, due to a significant increase in the mean degree of polymerization of the cell wall tannins. The tannin composition was typical of grape seed tannins and was quite similar in the two cell fractions studied, but the epicatechin gallate proportion was significantly higher in the cell wall fraction. There were no significant effects of water deficit on composition and polymerization of seed tannins. 相似文献
55.
56.
Vermoote M Pasmans F Flahou B Van Deun K Ducatelle R Haesebrouck F 《Veterinary microbiology》2011,153(3-4):339-342
Helicobacter suis is a very fastidious porcine gastric pathogen, which is also considered to be of zoonotic importance. In vitro antimicrobial susceptibility cannot be determined using standard assays, as this agent only grows in a biphasic medium with an acidic pH. Therefore, a combined agar and broth dilution method was used to analyse the activity of nine antimicrobial agents against nine H. suis isolates. After 48 h microaerobic incubation, minimal inhibitory concentrations (MICs) were determined by software-assisted calculation of bacterial growth. Only for enrofloxacin a bimodal distribution of MICs was demonstrated, indicating acquired resistance in one strain, which showed an AGT→AGG (Ser→Arg) substitution at codon 99 of gyrA. In conclusion, the assay developed here is suitable for determination of the antimicrobial susceptibility of H. suis isolates, although activity of acid sensitive antimicrobial agents may be higher than predicted from MIC endpoints. 相似文献
57.
Camila Cané-Retamales Freddy Mora Felipe Vargas-Reeve Sandra Perret Rodrigo Contreras-Soto 《Euphytica》2011,178(2):177-183
In the dry regions of Chile, prolific flowering from forest plantation is particularly advantageous for honey production,
in order to supplement the erratic flowering in native plants. Eucalyptus cladocalyx is a species suitable for areas with low water availability and their flowers provide a reliable source for the production
of honey. The aim of this study was to examine the heritability of flowering intensity in 49 open-pollinated families of E. cladocalyx in southern Atacama Desert, Chile, with the view to the selection for prolific flowering, but with minimal impact on precocious
flowering. The Bayesian variance component estimation model was assumed using the Gibbs sampling algorithm. Threshold models
were fitted to flowering data (bi-character model). Flowering intensity was found to be highly heritable (posterior mean:
h
2 = 0.48; and credible interval: 0.41–0.56). The posterior mean of the genetic correlation between flowering precocity and
intensity was positive (r = 0.45) and according to the credible interval (0.341–0.542), it was significantly different from zero, indicating that selection
on breeding values of early flowering at age three, would have significant and positive impact on flowering intensity 5 years
later (or in 8-year-old trees). These results are important for the start of a small-scale breeding program for the species
in southern Atacama Desert. The genetic variability found in these breeding populations may be used for breeding purposes
in regions where arid environmental conditions are limiting to the establishment of eucalypts trees. 相似文献
58.
De Buck J Van Immerseel F Meulemans G Haesebrouck F Ducatelle R 《Veterinary microbiology》2003,93(3):223-233
The ability of Salmonella enterica serotype Enteritidis isolates to adhere to immobilized secretions of the isthmus of the laying hen was determined in an ELISA-type assay. One-third of the 56 isolates tested in the logarithmic growth phase, adhered to the isthmal secretions. Using a binding assay of the isolates to thin paraffin sections of the oviduct, we demonstrated that the receptor of the adhesion was localized inside the tubular gland cells of the isthmus. The adhesion to immobilized isthmal secretions as well as to the paraffin sections was blocked by the addition of mannose. A fimD mutant of S. Enteritidis, lacking type 1 fimbriae, did not adhere, confirming that the adhesion was mediated by type 1 fimbriae. Mannosylated glycoproteins were demonstrated in the isthmus glandular cells using confocal laser scanning microscopy by FITC-labelled Lens culinaris lectins. It is hypothesized that the binding of S. Enteritidis to isthmal secretions could play a role in the contamination of eggs through incorporation of the bacteria in the shell membranes. 相似文献
59.
Annelies Maria Declercq Freddy Haesebrouck Wim Van den Broeck Peter Bossier Annemie Decostere 《Veterinary research》2013,44(1):27
Flavobacterium columnare (F. columnare) is the causative agent of columnaris disease. This bacterium affects both cultured and wild freshwater fish including many susceptible commercially important fish species. F. columnare infections may result in skin lesions, fin erosion and gill necrosis, with a high degree of mortality, leading to severe economic losses. Especially in the last decade, various research groups have performed studies aimed at elucidating the pathogenesis of columnaris disease, leading to significant progress in defining the complex interactions between the organism and its host. Despite these efforts, the pathogenesis of columnaris disease hitherto largely remains unclear, compromising the further development of efficient curative and preventive measures to combat this disease. Besides elaborating on the agent and the disease it causes, this review aims to summarize these pathogenesis data emphasizing the areas meriting further investigation. 相似文献
60.
Rapid endothelialization is important for biodegradable blood-contacting devices not only to prevent thrombosis but also to prevent degradation debris from entering the bloodstream and causing further complications. Here the authors report a three-step surface modification method, by which biomolecules, such as gelatin and chitosan, are covalently immobilized on the surface of plasma-treated poly(L-lactic acid) (PLLA) via -COOH groups introduced by acrylic acid grafting polymerization. Surface characterization techniques, including x-ray photoelectron spectroscopy, contact angle measurement, and colorimetric methods for surface density of functional groups, proved the feasibility and stability of this surface modification method. Surface wettability was increased by biomolecules immobilization. The -COOH surface density was measured to be 4.17±0.15?μmol/cm(2), the and amount of gelatin immobilized was 4.8?μg/cm(2). Human umbilical vein endothelial cell was used during in vitro study at seeding density of 10(4)?cells/cm(2). PLLA-gAA-gelatin surface was found to enhance cell adhesion, spreading, focal adhesion formation, and proliferation significantly. Chitosan-modified PLLA shows marginally improvement in cell adhesion and proliferation. Endothelialization was achieved within 7 days on both modified PLLA surfaces. In conclusion, this work demonstrates the feasibility of the surface modification method, and its ability to promote complete endothelialization for cardiovascular applications. 相似文献