Patterns of Follicular Growth in Superovulated Sheep and Influence on Endocrine and Ovarian Response     

A Gonzalez-Bulnes  RM Garcia-Garcia  CJH Souza  J Santiago-Moreno  A Lopez-Sebastian  MJ Cocero  DT Baird 《Reproduction in domestic animals》2002,37(6):357-361

Objectives of this study were to characterize patterns of follicular development in sheep superovulated with purified follicle stimulating hormone (FSH) (OVAGEN^TM, ICP, Auckland, New Zealand) and to determine its influence on preovulatory events (onset of the oestrus behaviour and timing of the preovulatory luteinizing hormone surge) and ovarian response (ovulation rate and embryo yield). Number and size of all ≥ 23 mm follicles from the first FSH injection to withdrawal of progestagen sponges was determined by transrectal ultrasonography just prior to every FSH injection in nine Manchega ewes superovulated with eight decreasing doses (ml) (1.5 × 3, 1.25 × 2 and 1 × 3) of OVAGEN injected twice daily from 60 h before to 24 h after the withdrawal of 40 mg fluorogestone acetate sponges. Oestrous detection and jugular blood sampling for LH radioimmunoassay were performed every 3 h from 14 to 53 h after sponge removal and ovulation rate and number of embryos were determined 4 days after progestagen withdrawal. Administration of OVAGEN induced a significant rise (p < 0.0005) in the number of follicles ≥ 4 mm in size because of an increased growth in size of follicles from the first FSH injection to sponge removal, an increase in the number of newly detected follicles from 12 to 36 h of the first FSH dose (p < 0.005) and a decrease in regression rate from 24 h (p < 0.001). The number of follicles 2–3 mm in size at first FSH dose (10.4 ± 1.5) was positively correlated with the number of ≥ 4 mm follicles at 0 h (19.0 ± 2.7, p < 0.01). A higher number of ≥ 4 mm follicles at 0 h was related with an earlier appearance of oestrus (31.5 ± 1.5 h, p = 0.08) and LH surge (45.0 ± 2.3 h, p < 0.005), and a higher ovulation rate (18.2 ± 3.8, p < 0.005). On the other hand, the rate of embryo recovery was decreased in ewes with earlier preovulatory LH peaks (p < 0.005), with a shorter interval between oestrus and LH peak (p < 0.05).  相似文献   

The 1872 outbreak of foot-and-mouth disease in Australia why didnt it become established?     

CM BUNN  MG GARNER  RM CANNON 《Australian veterinary journal》1998,76(4):262-269

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Expression profiles of immunity and reproductive genes during transition period in Holstein cattle          下载免费PDF全文

AI Ateya  MS Hussein  HM Ghanem  RM Saleh  WB El‐Domany  YY Elseady 《Reproduction in domestic animals》2018,53(2):352-358

The transition period is a critical time for dairy cows as the animal is subjected to the physiological stress accompanying parturition. Immunosuppression and health status were examined during this period in 80 Holstein cows. Blood samples were taken from each cow 3, 2 and 1 week before and after calving, and at calving (0 day). RNA was extracted and subjected to real‐time PCR to determine mRNA levels for the immune‐related genes TLR 2, 4, 6, 7 and β‐defensin 5 in addition to the reproduction‐related genes prolactin and IGF‐I. Results showed significant up‐regulation of pro‐inflammatory‐selected genes, TLR 4, 6 7 and β‐defensin 5 at the third‐week post‐calving; however, earlier periods had lower expression of such genes. In contrast, the immunosuppression biomarker TLR2 gene was up‐regulated at calving and 1 week after parturition and then down‐regulated again at second and third week. Prolactin and IGF‐I genes expression levels were significantly and gradually increased mainly post‐partum. This research highlights that the expression patterns of TLRs, BNBD5, PRL and IGF‐I could be biomarkers to follow up immune and reproductive status of dairy cow at peri‐parturient period to predict the most susceptible risk time for disease incidence and to build up management protocol.  相似文献   

Retracted: Influence of Glutamine Supplementation on Motility and Fertilization Success of Frozen–Thawed Persian Sturgeon (Acipenser persicus) Sperm          下载免费PDF全文

MS Aramli  K Golshahi  RM Nazari  A Golpour  S Aramli 《Reproduction in domestic animals》2016,51(4):474-477

Amino acids have an important biological role for the prevention of cell damage during cryopreservation. The aim of this study was to investigate the effects of glutamine on post‐thaw sperm motility and fertilization success in the Persian sturgeon (Acipenser persicus). Sperm collected from six fish was cryopreserved in extenders containing different glutamine concentrations (2.5, 5 and 10 mm ). Sperm samples diluted at the ratio of 1 : 1 using the extenders were subjected to cryopreservation. After dilution, the sperm suspensions were sucked into 250‐μl straws; the straws were placed on the tray, frozen in nitrogen vapour and plunged into liquid nitrogen. Then, sperm were thawed in a water bath at 40°C for 5 s and used for analysis. Our results revealed that an increase in the concentration of glutamine caused a significant increase in the motility percentage, curvilinear velocity (VCL) and also fertilization success in the Persian sturgeon (p < 0.05). Comparing all concentrations of glutamine, the best concentration for sperm motility and fertilization rate was 10 mm . In addition, higher post‐thaw motility percentage, VCL, and fertilization and hatching rates were obtained with the extender at the concentration of 10 mm (p < 0.05). The findings of this study showed that glutamine was of greater benefit to Persian sturgeon sperm motility during frozen–thawed process.  相似文献   

Influence of Gonadotrophin‐Induced First Oestrus on Gilt Fertility     

DM Hidalgo  RM Friendship  L Greiner  R Manjarín  MR Amezcua  JC Domínguez  RN Kirkwood 《Reproduction in domestic animals》2014,49(6):899-902

The aim of this study was to determine the association between the oestrous response of pre‐pubertal gilts to gonadotrophin injection or boar exposure and their subsequent farrowing rate and litter size. At 154 days of age, randomly selected pre‐pubertal gilts received an intramuscular injection of 400 IU equine chorionic gonadotrophin plus 200 IU human chorionic gonadotrophin (PG600^®; Merck Animal Health; n = 181). From the remaining pool of animals not treated with hormones, the first gilts showing signs of oestrus were selected to act as controls (n = 201). Boar exposure began at 155 days of age for both groups, and gilts were bred at a weight of approximately 130 kg. Comparisons were made between PG600^®‐treated gilts exhibiting oestrus or not within 7 days post‐injection (early and late responders, respectively) and control gilts exhibiting oestrus or not within 30 days after beginning of boar exposure (select and non‐select control gilts, respectively). By 162 days, oestrus was detected in 67.5% of PG600^®‐treated gilts compared with 5.7% of control gilts (p < 0.0001). The proportion of animals observed in oestrus at least three times before breeding was greater for select control gilts compared with early and late responder PG600^®‐treated gilts (p ≤ 0.001). There were no significant differences in farrowing rate and litter size between the four treatment groups. These data indicate that PG600^® is an effective tool to induce an earlier oestrus in gilts, that subsequent farrowing rate and born alive litter size compare favourably to that of select gilts and that gilts failing to respond promptly to hormonal stimulation do not exhibit compromised fertility.  相似文献   

Effect of eCG or eCG Plus hCG on Oestrus Expression and Ovulation in Prepubertal Gilts     

R Manjarin  G Cassar  DJ Sprecher  RM Friendship  JC Dominguez  RN Kirkwood 《Reproduction in domestic animals》2009,44(3):411-413

To meet weekly breeding targets, it is occasionally necessary to inject exogenous gonadotrophins to induce oestrus in prepubertal gilts. However, the gilt oestrus response to equine chorionic gonadotrophin (eCG) either alone or in combination with human chorionic gonadotrophin (hCG) can be unpredictable. The objective of the present study was to examine possible reasons for this unpredictability. Prepubertal gilts (90 kg and 153 days of age, n = 109) received an injection of either 600 IU eCG or a combination of 400 IU eCG and 200 IU hCG (PG600), or were non-injected controls, and were then exposed to a mature boar for 15 min daily for 7 days for oestrus detection. At the time of injection, real-time ultrasound revealed that the gilt ovaries had primarily 1–2 mm follicles. Blood samples were obtained at time of hormone injection (day 0) and at days 3, 7 and 10 for assay of serum progesterone concentrations. The oestrus responses by 7 days were15.5%, 73.3% and 0%, for eCG, PG600, and control gilts, respectively (p < 0.001). The oestrus response improved (p < 0.05) with increasing body weight. Based on circulating progesterone levels, all oestrous gilts ovulated except for four of the PG600 gilts. Failure to express oestrus in PG600 gilts was not associated with a premature rise in progesterone.  相似文献   

Taxonomy and evolution of cultivated plants: Literature review 1984/1985     

Jürgen Schultze-Motel  Reinhard Fritsch  Karl Hammer  Peter Hanelt  Joachim Kruse  Helga I. Maass  Harald Ohle  Klaus Pistrick 《Genetic Resources and Crop Evolution》1986,34(2):335-347

Summary Important papers on taxonomy and evolution of cultivated plants published in 1984 and 1985 were compiled and briefly discussed.

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Taxonomie und Evolution der Kulturpflanzen: Literaturübersicht 1984/1985

Zusammenfassung Wichtige Arbeiten über Taxonomie und Evolution der Kulturpflanzen aus den Jahren 1984 und 1985 wurden zusammengestellt und kurz kommentiert.

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: 1984–1985

, 1984 1985 . .

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Transformation of haematite and Al-poor goethite to Al-rich goethite and associated yellowing in a ferralitic clay soil profile of the middle Amazon Basin (Manaus, Brazil)   总被引：1，自引：0，他引：1  

E. Fritsch  G. Morin  A. Bedidi  D. Bonnin  E. Balan  S. Caquineau  & G. Calas 《European Journal of Soil Science》2005,56(5):575-588

The red and yellow colours of ferralitic soils in the tropics have for long intrigued pedologists. We have investigated the upward yellowing in a 10-m thick profile representative of the Ferralsols of the plateaux of the Manaus region of Brazil. We determined changes in the nature and crystal chemistry of their Fe oxides by optical and Mössbauer spectroscopy as well as Rietveld refinement of X-ray diffraction patterns. We attribute the upward yellowing of the soil to a progressive transformation of the Fe oxides at nearly invariant iron contents. Aluminium in contrast is strongly mobilized in the uppermost clay-depleted topsoil where there is preferential dissolution of kaolinite and crystallization of gibbsite. Haematite decreases from 35 to 10% of the Fe oxides from the bottom to the top of the profile and the particles become smaller (75–10 nm). Its Al for Fe-substitution remains almost unchanged (10–15 mol %). The average Al-substitution rate of goethite increases from 25 to 33 mol %, and its mean crystal diameter remains in the range 20–40 nm. The proportion of Al-rich goethite (33 mol %) increases at the expense of less Al-substituted Fe oxides (haematite and goethite). This conversion with restricted transfer of iron means that the amount of Al stored in Fe oxides gradually increases. Kaolinite, haematite and Al-poor goethite are thus witnesses of earlier stages of ferralitization of the soil. In contrast, Al-rich goethite and gibbsite initiate the alitization (or bauxitization) of the soil. They correspond to the last generation of soil minerals, which most likely reflects the present-day weathering conditions. The progressive replacement of kaolinite, haematite and Al-poor goethite by new generations of Al-rich goethite and gibbsite attests to greater activities of water and aluminium and smaller activity of aqueous silica in the topsoil than in the subsoil. We interpret this as a consequence of longer periods of wetting in the topsoil that could result from soil aging, more humid climate or both.  相似文献   

Collection of indigenous taxa of cultivated plants in the Georgian SSR 1985     

Rusudan K. Beridze  Reinhard Fritsch  Verniko N. Kandelaki  Džulietta Mandžgaladze  Klaus Pistrick  Natela Taralašvili 《Genetic Resources and Crop Evolution》1986,34(2):305-316

Summary In continuation of joint activities since 1981 in Western Georgia a further mission for studying and collecting indigenous forms of cultivated plants and their wild relatives has been carried out by staff members of the Institute of Botany, Tbilisi, and the Zentralinstitut für Genetik und Kulturpflanzenforschung, Gatersleben, in September/October 1985. 445 accessions, mainly of legumes, vegetables, spice plants and maize could be collected in South-western Georgia (Adaria, area around Boromi). Gene-erosion is highly advanced for field crops (especially cereals), may be as a result of the specialization to subtropical cultures near the coast and to tobacco, respectively cattle-breeding in higher mountainous regions. But many local races of garden crops are still cultivated now. A large amount of indigenous taxa of cultivated plants, of potential value for plant breeding and botanically interesting, could be obtained. The collection work in the Georgian SSR should be continued.

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Sammlung indigener Kulturpflanzensippen in der Georgischen SSR 1985

Zusammenfassung In Fortführung der 1981 begonnenen gemeinsamen Unternehmungen im westlichen Georgien führten Mitarbeiter des Instituts für Botanik Tbilisi und des Zentralinstituts für Genetik und Kulturpflanzenforschung Gatersleben im September/Oktober 1985 eine weitere Reise zum Studium und zur Sammlung indigener Kulturpflanzenformen und verwandter Wildsippen durch. Im südwestlichen Georgien (Adarien, Gebiet um Boromi) konnten 445 Proben, hauptsächlich von Leguminosen, Gemüse- und Gewürzpflanzen sowie Mais, gesammelt werden. Infolge der Spezialisierung auf subtropische Kulturen in Künstennähe und auf den Anbau von Tabak bzw. auf die Viehzucht in den höheren Gebirgsregionen ist die Generosion bei Feldkulturen, insbesondere bei den Getreiden, weit fortgeschritten. Dagegen sind noch viele Landsorten gärtnerischer Kulturen im Anbau. Eine große Menge für die Pflanzenzüchtung potentiell wertvoller und botanisch interessanter indigener Kulturpflanzensippen wurde erfaßt. Die Sammeltätigkeit in der Georgischen SSR sollte fortgesetzt werden.

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1985

1981 , — 1985 () () . - ( ) 445 , , , . , , . , . , , . .

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Taxonomy and evolution of cultivated plants: Literature review 1987/1988     

Jürgen Schultze-Motel  Reinhard Fritsch  Karl Hammer  Peter Hanelt  Joachim Kruse  Helga I. Maass  Harald Ohle  Klaus Pistrick 《Genetic Resources and Crop Evolution》1989,37(2):453-469

Summary Important papers on taxonomy and evolution of cultivated plants published in 1987 and 1988 were compiled and briefly discussed.

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Taxonomie und Evolution der Kulturpflanzen: Literaturübersicht 1987/1988

Zusammenfassung Wichtige Arbeiten über Taxonomie und Evolution der Kulturpflanzen aus den Jahren 1987 und 1988 wurden zusammengestellt und kurz kommentiert.

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¶rt; : 1987/1988

, 1987/1988 . .

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