Evaluation of parameters obtained from two systems of gait analysis     

ND Assaf  SC Rahal  LR Mesquita  WT Kano  RB Abibe 《Australian veterinary journal》2019,97(10):414-417

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Induction of apoptosis on K562 cell line and double strand breaks on colon cancer cell line expressing high affinity receptor for granulocyte macrophage-colony stimulating factor (GM-CSF)     

Roudkenar MH  Bouzari S  Kuwahara Y  Roushandeh AM  Baba T  Oloomi M  Fukumoto M 《Iranian Biomedical Journal》2008,12(1):1-6

BACKGROUND: Immunotoxins are comprised of both the cell targeting and the cell killing moieties. We previously established a new immunotoxin, i.e. Shiga toxin granulocyte macrophage-colony stimulating factor (StxA1-GM-CSF), comprises of catalytic domain of Stx, as a killing moiety and GM-CSF, as a cell targeting moiety. In this study, the ability of the immunotoxin to induce apoptosis and double strand breaks (DSB) on different cell lines was investigated. METHODS: The recombinant hybrid protein was expressed in bacterial expression system and purified with nickel-nitrilotriacetate acid resin. The K562 (erythroid leukemia) cell line and LS174 (colon carcinoma) were used in this study. The neutral comet assay was carried out for the detection of DSB and Hoechst staining was performed for apoptosis. RESULTS: StxA1-GM-CSF effectively induced apoptosis on K562 cell line and DNA Double Strand Break (DSB) were observed on colon cancer cell line treated with StxA1-GM-CSF. CONCLUSION: This novel action i.e. DNA damage might be a relevant mechanism of action for StxA1-GM-CSF that is designed to act as immunotoxin, although further investigation is required.  相似文献   

Molecular cloning and characterization of a thaumatin-like protein-encoding cDNA from rough lemon     

Bong-Gyu Kim  Takeshi Fukumoto  Satoshi Tatano  Kenji Gomi  Kouhei Ohtani  Yasuomi Tada  Kazuya Akimitsu 《Physiological and Molecular Plant Pathology》2009,74(1):3-10

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Molecular cloning and expression analysis of cDNAs encoding a putative Nrt2 nitrate transporter from peach     

Nakamura Y  Umemiya Y  Masuda K  Inoue H  Fukumoto M 《Tree physiology》2007,27(4):503-510

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Genetic structure and diversity of the Banana bunchy top virus population on Sumatra Island,Indonesia     

Yuya Chiaki  Nasril Nasir  Henny Herwina  Jumjunidang  Ayaka Sonoda  Tomohiro Fukumoto  Masayuki Nakamura  Hisashi Iwai 《European journal of plant pathology / European Foundation for Plant Pathology》2015,143(1):113-122

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Induction of superovulation using inhibin antiserum and competence of embryo development in wild large Japanese field mice (Apodemus speciosus)     

Kanna Meguro  Kazuki Komatsu  Takuya Ohdaira  Naomi Nakagata  Akifumi Nakata  Manabu Fukumoto  Tomisato Miura  Hideaki Yamashiro 《Reproduction in domestic animals》2019,54(12):1637-1642

Seasonally, bred wild mice provide a unique bioresource, with high genetic diversity that differs from wild‐derived mice and laboratory mice. This study aimed to establish an alternative superovulation method using wild large Japanese field mice (Apodemus speciosus) as the model species. Specifically, we investigated how the application of inhibin antiserum and equine chorionic gonadotropin (IASe) during both the reproductive and non‐reproductive seasons impact the ovulation rate and competence of embryo development after in vitro fertilization (IVF) with fresh and cryopreserved sperm. When the wild mice were superovulated by injecting eCG followed by human chorionic gonadotropin (hCG), few oocytes were collected during the reproductive and non‐reproductive seasons. In comparison, the number of ovulated oocytes was dramatically enhanced by the administration of IASe, followed by isolation of ovulated oocytes 24 hr after 30 IU hCG administration. The IVF oocytes that were in vitro cultured (IVC) with medium containing serum further developed to the 2‐ and/or 4‐cell stage using both fresh and frozen‐thawed sperm. In conclusion, we successfully established an alternative protocol for collecting ovulated oocytes from wild large Japanese field mice by administering IASe and hCG during both the reproductive and non‐reproductive seasons. This study is the first to develop IVF–IVC wild large Japanese field mice beyond the 2‐ and/or 4‐cell stage in vitro using fresh and cryopreserved sperm. This approach could be used in other species of wild or endangered mice to reduce the number of animals used for experiments, or in maintaining stocks of germ cells or embryos.  相似文献   

岩棉营养液栽培K,N不同浓度对番茄生育,产量及品质的影响   总被引：4，自引：0，他引：4  

周艺敏  吉田彻志 《华北农学报》1997,12(2):107-114

采用岩棉营养液栽培方法探讨不同Ｋ、Ｎ水平对番茄的生育，产量及品质的影响，试验结果表明Ｋ、Ｎ配合明显促进番茄生育，单株鲜重，干物重，茎粗，叶面积均随营养液中Ｋ浓度的增加而增大，呈显著或极显著正相关（ｒ＝０．９３９～０．９９８）。Ｎ、Ｋ互作未达显著性水准，但Ｎ浓度的增加提高了相关关系的斜率，随Ｋ浓度的增加，番茄开花和座果及成熟期提前，单株产量，单株结果数，单果重有增加倾向；优质果率明显提高，品质分析结  相似文献   

Effects of thiosulfate addition on ammonia and nitrogen removal in biofilters packed with Oyaishi (pumice tuff)     

Tomoko Yasuda  Yasuyuki Fukumoto  Miyoko Waki  Toshimi Matsumoto  Hirohide Uenishi 《Animal Science Journal》2020,91(1)

Ammonia removal is achieved partly by absorption and nitrification in biofilters, resulting in the accumulation of nitrogen and the necessity of treating the effluent water. We investigated the effects of thiosulfate addition to a biofilter containing pumice tuff for ammonia and nitrogen removal in a laboratory‐scale experiment. The addition of thiosulfate to the circulating water led to a decreased nitrate and nitrite along with an increase of sulfate. The inorganic nitrogen in the circulating water decreased by up to 44% with thiosulfate addition compared to without thiosulfate. Batch experiments revealed that denitrification activity decreased exponentially along with increases in dissolved oxygen; however, approximately 30% of denitrification activity was maintained at dissolved oxygen concentration of 3.3 mg/L. Metabarcoding of 16S rRNA genes indicated that the genus Thiobacillus had a relative abundance of 0.002%–0.016% of total bacteria in the biofilter packing material. The circulating water pH was decreased below 5 with sulfur oxidation, and ammonium was accumulated without pH control resulting in a decrease in the relative abundance of the family Nitrosomonadaceae. Its relative abundance increased with control of pH to near neutral, indicating that ammonia‐oxidizing activity could be maintained by adjusting pH. Thiosulfate addition could stimulate nitrogen removal by sulfur‐dependent denitrification in biofiltration systems.  相似文献   

Effect of Meloxicam on Pregnancy Rate of Recipient Heifers Following Transfer of In Vitro Produced Embryos     

TS Aguiar  CV Araújo  RR Tirloni  LR Martins 《Reproduction in domestic animals》2013,48(6):984-988

The main objective of this study was to determine if administration of meloxicam, a cyclooxygenase (COX) two inhibitor, to heifers in which embryo transfer (ET) is more difficult and requires a greater manipulation of the tract, would be beneficial. Nulliparous recipient heifers were divided in two groups: CON (n = 102), in which animals received 10 ml of saline IM (the same volume of meloxicam) and MEL (n = 105) animals that were treated with meloxicam. According to the degree in passing the catheter, recipients from both groups were classified as Grade I, easy (< 60 s), and Grade II (more than 80 s), difficult. Immediately after embryo transfer, MEL recipients received an injection of 200 mg of meloxicam (10 ml).There was no difference in the pregnancy rates on Day 35 considering animals which presented Grade I cervix independently whether the treatment was performed or not (p = 0.22). There was a statistical difference in the pregnancy rates (p < 0.01) between both groups (49.0% and 66.7% for CON and MEL, respectively) when cervical grade was not considered, on Day 35. Considering the animals that presented Grade II cervix, the pregnancy rate was higher for MEL (21.15% and 78.84%, respectively) in both examinations (p < 0.01).The authors concluded that meloxicam had a positive influence on general pregnancy rate of treated heifers in comparison to non‐treated heifers. It was also observed that pregnancy rate was not influenced by meloxicam administration in Grade I heifers. Treatment increased the pregnancy rate of Grade II heifers.  相似文献   

Effect of CaCl2 treatment on the acclimatisation of mericlone seedlings of Cattleya and Phalaenopsis     

Jincai Li  Xiheng Zhao  Yasuyo Nishimura  Yasufumi Fukumoto 《The Journal of Horticultural Science and Biotechnology》2013,88(5):445-450

Summary

To increase resistance to environmental stress during the acclimatisation of mericlone seedlings of Cattleya and Phalaenopsis, seedlings were treated with CaCl₂. The effectiveness of CaCl₂ treatment on acclimatisation was determined by investigating the levels of anti-oxidative enzyme activity and reactive oxygen species (ROS), the amounts of chlorophyll and malondialdehyde (MDA), the net rate of photosynthesis (A), and the area of leaf-burn caused by high temperature injury. The activities of four anti-oxidative enzymes [superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and peroxidase (POD)] in mericlone seedlings of Cattleya and Phalaenopsis increased with CaCl₂ treatment. The highest increase was in the treatment with 4 mM CaCl₂. Mericlone seedlings treated with 4 mM CaCl₂ had increased SOD, CAT, APX, and POD activities compared with the controls after 35 d of acclimatisation in a greenhouse. The levels of superoxide anions (O₂^?–) and hydrogen peroxide (H₂O₂) were also low. In line with this, any decrease in chlorophyll content, increase in MDA content, drop in A, or injury due to high temperature stress were mitigated by CaCl₂ treatment. These results show that CaCl₂ treatment is effective for the acclimatisation of mericlone seedlings of Cattleya and Phalaenopsis by increasing their resistance to environmental stress.  相似文献