Effects of polyphenols from seed shells of Japanese horse chestnut (Aesculus turbinata BLUME) on methotrexate-induced intestinal injury in rats     

Sugiyama A  Kimura H  Ogawa S  Yokota K  Takeuchi T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(5):673-678

The purpose of this study was to evaluate the effects of polyphenols from seed shells of Japanese horse chestnut (JHP) on methotrexate (MTX)-induced intestinal injury in rats. MTX application caused intestinal morphological injury and increase in malondialdehyde (MDA) levels, decrease in levels of glutathione (GSH) and glutathione peroxidase (GSH-Px) activities in small intestine. However, oral administration of JHP ameliorated MTX-induced intestinal injury and inhibited the increase in MDA and the decrease in GSH and GSH-Px activity in small intestine. In conclusion, our results indicated that oral administration of JHP alleviated MTX-induced intestinal injury through its antioxidant properties.  相似文献   

Immunohistochemical detection of phosphatidylserine and thrombospondin on denucleating erythroblasts in rat bone marrow     

Yokoyama T  Etoh T  Udayanga KG  Takahara E  Qi WM  Warita K  Matsumoto Y  Miki T  Takeuchi Y  Hoshi N  Kitagawa H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(7):949-952

The hypothesis that apoptotic factors play some roles in the denucleation of erythroblasts has been confirmed by the immunohistological detection of both phosphatidylserine and thrombospondin as phagocytosis-inducing factors in general apoptotic events. Both phosphatidylserine and thrombospondin were detected on the surface of cell membrane of mature erythroblasts, while thrombospondin was also detected in more immature erythroblasts. The intensities of their immune reactions increased as the erythroids matured. During denucleation, the positivities of both phosphatidylserine and thrombospondin were restricted on the surface of the cell membrane surrounding the protruding nuclei. Thus, the apoptotic process involves denucleation of erythroblasts and phosphatidylserine, and thrombospondin acts as phagocytosis-inducing factors in the denucleation event.  相似文献   

A putative TetR-type transcription factor AZC_3265 from the legume symbiont Azorhizobium caulinodans represses the production of R-bodies that are toxic to eukaryotic cells     

Jun-ichi Matsuoka  Fumiko Ishizuna  Keigo Kurumisawa  Kengo Morohashi  Tetsuhiro Ogawa  Makoto Hidaka 《Soil Science and Plant Nutrition》2013,59(5):452-459

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Stimulatory effect of aluminum on the growth of tea pollen tubes     

Hiromi Yokota  Ikuyo Takamura  Fumiko Ishikawa  Miwako Ohta  Shigeki Konishi 《Soil Science and Plant Nutrition》2013,59(2):457-461

It is well known that when inorganic elements such as boron (B), calcium, cobalt, and manganese are added to media in combination or alone, they stimulate the germination and/or tube growth of various kinds of pollen (Schmücker 1933; Loo and Hwang 1944; Yamada 1958; Brewbaker and Kwack 1963). Among these elements, the stimulatory effect ofB is the most effective and generally recognized. Aluminum (Al) belongs along with B to Group IIIa in the periodic table, and Fähnrich and Ultlich (1964) reported that Al inhibited the germination and growth of the pollen tube of Petunia hybrida and Antirrhinum majus. However, stimulatory effects of Al on pollen germination or pollen tube growth had not been previously reported.  相似文献   

Fuzzy optimization model for integrated management of total nitrogen loads from distributed point and nonpoint sources in watershed     

Shigeya Maeda  Toshihiko Kawachi  Koichi Unami  Junichiro Takeuchi  Tomoki Izumi  Syunsuke Chono 《Paddy and Water Environment》2009,7(3):163-175

A fuzzy optimization model is developed to allocate allowable total nitrogen (T-N) loads to distributed nonpoint sources (NPSs) and point sources (PSs) in a watershed for river water quality management using the linear programing technique. The watershed is divided into uniform grid cells on which T-N loads issuing from NPSs such as paddy fields, upland crop fields and cities are controlled. A geographic information system integrated with the digital elevation model facilitates computation of route lengths of surface and subsurface flows from cells to a river running through the watershed. The T-N loads discharged from their sources are assumed to decay, subject to distance-related first-order kinetics. As management goals, maximizations of total allowable NPS loads, total allowable PS loads and total yield of rice are considered from environmental and economic viewpoints. A prime constraint is an effluent limitation standard for the aggregate amount of loads that arrive at the downstream end of the river. The fuzzy sets theory helps appropriately describe vague attitudes of decision-makers (i.e., stakeholders and management authorities) in terms of constraints and conflicting goals. An application of the fuzzy optimization model, developed as an improvement over our last nonfuzzy model, to a real watershed in Shiga prefecture, Japan, demonstrates that the fuzzy model embodies our last model, and is capable of creating management alternatives for T-N load allocation in a more practical and flexible manner.  相似文献   

A distributed hydro-environmental watershed model with three-zoned cell profiling   总被引：1，自引：1，他引：0  

Junichiro Takeuchi  Toshihiko Kawachi  Koichi Unami  Shigeya Maeda  Tomoki Izumi 《Paddy and Water Environment》2009,7(1):33-43

A cell-based distributed watershed model is developed which enables us to simulate the hydrological and hydraulic aspects of the watershed in a refined fashion. With three-zoned cell profiling, the model is composed of three sub-models; tank model for a surface water zone, soil moisture model for a surface soil zone, and unconfined shallow groundwater flow model for a subsurface zone. Inclusion of the soil moisture sub-model modified to reroute the infiltration, routed from the tank sub-model, into the return flow and the groundwater recharge features the model. The groundwater flow sub-model, numerically approximated by use of the finite volume method and the implicit time-marching scheme, considers a network of on-farm drainage canals as internal boundaries, which is an essential need for modeling the watershed including farmlands. Cascade-linking of the three sub-models in a cell and assembling of all the cells over the entire watershed domain provides the global equations system to be solved. Applicability of the model is demonstrated with its practical application to a real watershed in that paddy and upland crop fields take great part of the land-use practice. It is then indicated in a quantified manner that rice farming significantly contribute as a major groundwater recharger in an irrigation period to fostering and conservation of regional water resources. Along with appropriately profiling a cell, the model is so versatile and tough that it can be applied without difficulty to a watershed of diverse terrains and land-uses and the computations can stably be carried out. It is thus concluded that the model presently developed could be a powerful “watershed simulator” to investigate and assess the time-varying hydro-environmental properties of a watershed while separating and integrating the hydrological and hydraulic components of particular importance.  相似文献   

Genome editing of Nf1, Pten,and Trp53 in neonatal mice induces glioblastomas positive for oligodendrocyte lineage transcription factor 2     

Hiromi Yamamoto  Keisuke Yamamura  Haruka Nagasaki  Takamasa Suzuki  Fumiko Ninomiya  Kenji Matsubara  Naomoto Harada  Shuichi Ohkubo 《Journal of toxicologic pathology》2021,34(4):359

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Effects of flooding depth on growth,morphology and photosynthesis in <Emphasis Type="Italic">Alnus japonica</Emphasis> species     

Fumiko Iwanaga  Fukuju Yamamoto 《New Forests》2008,35(1):1-14

The present study deals with effects of flooding depth on growth, morphology and photosynthesis in Alnus japonica species thorough one field study and two controlled experiments. In the field study performed in Kushiro Mire, Hokkaido Island, Japan, tree heights and stem diameters decreased with an increase in water depth accompanied with the reduction of soil redox potential. In contrast, the rate of multiple stems per individual tree increased. In the controlled experiments for seedlings flooding suppressed the shoot elongation and biomass increment in roots. However, diameter increment around water levels, epicormic shoot development and adventitious root formation were enhanced in flooded seedlings. The photosynthetic rate and stomatal conductance of flooded seedlings also were lowered with an increase in flooding depth. The recovery of the reduced photosynthetic rate and stomatal conductance occurred simultaneously with the advancement of adventitious root formation in the flooded seedlings. These results indicate the importance of a series of morphological changes occurring on stems around water levels in flood tolerance in A. japonica species.  相似文献   

Anatomical characterization of decayed wood in standing light red meranti and identification of the fungi isolated from the decayed area     

Erwin  Shuhei Takemoto  Won-Joung Hwang  Miyuki Takeuchi  Takao Itoh  Yuji Imamura 《Journal of Wood Science》2008,54(3):233-241

To further our understanding of wood decay in living light red meranti (Shorea smithiana) trees, microscopic characteristics of the cell and cell wall degradations of S. smithiana wood in the presence of the decay fungi, the identity of the causal fungi, and the decay potential and pattern by an isolated fungus were investigated. Cell wall degradations, including cell wall thinning, bore holes formation, rounded pit erosion, and eroded channel opening were clearly observed under light and scanning electron microscopy. In transverse view, many large voids resulting from a coalition of degraded wood tissue appeared in the decayed canker zone. All these observations suggest the well-known simultaneous decay pattern caused by white-rot fungi. By phylogenetic analysis based on the sequences of internal transcribed spacer region of ribosomal DNA, a basidiomycete fungus isolated from the decayed wood was identified as Schizophyllum commune. The degradation caused by this fungus on sound S. smithiana wood in an in situ laboratory decay test was classified as the early stage of simultaneous decay, and showed a similar pattern to that observed in the wood samples naturally decayed.  相似文献   

Biological effect of tyrosine kinase inhibitors on three canine mast cell tumor cell lines with various KIT statuses     

Takeuchi Y  Fujino Y  Fukushima K  Watanabe M  Nakagawa T  Ohno K  Sasaki N  Sugano S  Tsujimoto H 《Journal of veterinary pharmacology and therapeutics》2012,35(1):97-104

Tyrosine kinase inhibitors (TKIs) can be important in the treatment of canine mast cell tumor (cMCT). Meanwhile, some TKIs have been identified as substrates for ABCB1. The inhibitory effect of four TKIs (axitinib, imatinib, masitinib, and vatalanib) for proliferation and phosphorylation of c-Kit receptor as well as the expression and function of ABCB1 were investigated in three cMCT cell lines (HRMC, VIMC1, and CMMC1). The IC(50) values of the TKIs in HRMC, the only cell line with wild-type KIT, were clearly higher than those in CMMC1 and VIMC1. In HRMC and CMMC1, both the growth and phosphorylation of c-Kit receptor were suppressed proportionally by the TKIs. VIMC1 required higher concentrations for the inhibition of c-Kit receptor phosphorylation than those in cell growth. The treatment with cyclosporine increased the effects of the TKIs on VIMC1 since ABCB1 was expressed in VIMC1. The results indicated that cMCT cell lines harboring wild-type KIT had lower sensitivity to TKIs. The growth of VIMC1 was seemingly reduced by TKIs through the inhibition of other tyrosine kinases than c-Kit receptor. There was little influence of ABCB1 on TKI effects to the proliferation of VIMC1. These results will be helpful to understand the different sensitivity to TKIs in cMCT patients.  相似文献