Antioxidative Effect of Chlorella Pyrenoidosa Protein Hydrolysates and Their Application in Krill Oil-in-Water Emulsions     

Yujia Liu  Yuli Qi  Qi Wang  Fawen Yin  Honglei Zhan  Han Wang  Bingnan Liu  Yoshimasa Nakamura  Jihui Wang 《Marine drugs》2022,20(6)

Chlorella pyrenoidosa is an excellent source of protein, and in this research, we assessed the antioxidant and emulsifying effects of Chlorella protein hydrolysate (CPH) using neutral proteases and alkaline proteases, as well as the properties of CPH-derived krill oil-in-water (O/W) emulsions. The CPHs exhibited the ability to scavenge several kinds of free radicals, including 1,1-diphenyl-2-picrylhydrazyl (DPPH), O₂⁻, hydroxyl, and ABTS. Additionally, the CPHs (5 mg/mL) scavenged approximately 100% of the DPPH and ABTS. The CPHs showed similar emulsifying activities to Tween 20 and excellent foaming activities (max FS 74%), which helped to stabilize the krill oil-in-water emulsion. Less than 10 mg/mL CPHs was able to form fresh krill oil-in-water emulsions; moreover, the CPHs (5 mg/mL) in a krill O/W emulsion were homogenous, opaque, and stable for at least 30 days. Based on their inhibitory effects on the peroxide value (POV) and thiobarbituric acid reactive substances (TRABS), the CPHs were found to be able to inhibit lipid oxidation in both emulsifying systems and krill O/W emulsions. Thus, the CPHs could improve superoxide dismutase (SOD) activities by 5- or 10-fold and decrease the high reactive oxygen species (ROS) level caused by the addition of H₂O₂ in vitro. In conclusion, health-promoting CPHs could be applied in krill oil-in-water emulsions as both emulsifiers and antioxidants, which could help to improve the oxidative and physical stability of emulsions.  相似文献   

Ammonia volatilization from Vietnamese acid sulfate paddy soil following application of digested slurry from biogas digester     

Nakamura  Masato  Oritate  Fumiko  Yuyama  Yoshito  Yamaoka  Masaru  Dan  Nguyen Phuoc  Hanh  Dang Vu Bich 《Paddy and Water Environment》2018,16(1):193-198

Paddy and Water Environment - In some areas of Vietnam, digested slurry from biogas digesters is discharged into canals without wastewater treatment. In order to reduce environmental pollution,...  相似文献   

Isolation of salmonellae from apparently healthy fattening male dairy calves and fattening pigs and stability of plasmids in the isolates   总被引：1，自引：0，他引：1  

M Nakamura  K Ohmae  S Sato  S Suzuki  S Ikeda 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1985,47(3):379-384

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Histologic features of the carotid artery trifurcation in thoroughbreds.     

T Nakamura  K Kiryu  N Machida  T Iwata  M Oikawa  M Kaneko 《American journal of veterinary research》1992,53(3):288-290

The common, external, and internal carotid and occipital arteries were examined histologically at the trifurcation of the common carotid arteries in 13 Thoroughbred foals (0 to 30 days old) and 64 Thoroughbred adults (2 to 4 years old). Calcification in the media of the common carotid and external carotid arteries was observed in 3 of the 13 foals and in 30 of the 64 adult horses. Calcification resembled that seen in M?nckeberg's arteriosclerosis in human beings, the cause of which is unknown.  相似文献   

Effects of the components of Fusobacterium necrophorum in experimental liver abscess formation in mice     

Y Nakajima  K Nakamura  S Takeuchi 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1985,47(4):589-595

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Effect of proteolytic enzyme on experimental infection of mice with Pseudomonas aeruginosa.     

Y Tamura  S Suzuki  M Kijima  T Takahashi  M Nakamura 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(3):597-599

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Immunohistochemical studies on the antigen localization and the germinal center formation in mouse lymph nodes     

K Nakamura  Y Hashimoto  H Kitagawa  N Kudo 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1984,46(3):315-321

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Analysis of protein compositions and surface protein epitopes of Anaplasma centrale and Anaplasma marginale.     

Y Nakamura  S Kawazu  T Minami 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1991,53(1):73-79

Protein composition was compared and epitopes were analyzed among the isolates of Anaplasma centrale and A. marginale by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting using bovine antisera and monoclonal antibodies, and enzyme-linked immunosorbent assay. Common and unique proteins were found among the isolates. All isolates tested had a major surface protein with an apparent molecular weight of 38 to 40 kilodalton which had slight molecular size variations between species. This protein was also a dominant immunogen to the host. At least two species-common epitopes, one of which might contain carbohydrate(s), were present on the major surface protein. One species-specific epitope was identified on the major surface protein of A. marginale isolates.  相似文献   

Use of a beta 2-adrenergic stimulant (clenbuterol) for eliminating night-time calving.     

T Nakao  K Sato  T Nakamura  K Taguchi  M Moriyoshi  K Kawata 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(1):19-22

To determine whether beta 2-adrenergic stimulant (clenbuterol) would exhibit beneficial effects upon the elimination of night-time calving, 42 Holstein Friesian cows and heifers at the first stage of labour were injected intramuscularly twice with clenbuterol; 300 micrograms at 18:00 and 210 micrograms at 22:00. Other 26 cows and heifers of the same breed at the first stage of labour at 18:00 were not treated and served as controls. All of the treated animals, but one, calved after 5:00 in the next morning, showing a peak of parturition between 5:00 and 10:00. In contrast to this, 42% of the control animals calved at night between 22:00 and 5:00. Thus, the double administrations of clenbuterol were shown to be effective for eliminating night-time calving. The cows treated with clenbuterol tended to show lower incidences of dystocia and retained placenta and a higher first insemination conception rate than the control animals. No adverse effects of the treatment on the viability of newborn calves and milk yields were observed.  相似文献   

Demonstration of the correlation of a 36-megadalton Salmonella serovar enteritidis plasmid to virulence in mice by reintroduction of the plasmid   总被引：2，自引：0，他引：2  

S Suzuki  K Ohmae  M Nakamura  S Sato  T Koeda  K Ohishi  M Muramatsu 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1989,51(1):203-205

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