RNA干扰技术在蜱研究中的应用   总被引：1，自引：0，他引：1  

高晓  周金林 《中国动物传染病学报》2010,18(5)

蜱是仅次于蚊子的第二大疾病传播媒介,分布广泛,对人和动物的健康危害严重。RNA干扰技术已经成功应用于新秀丽小杆线虫、黑腹果蝇、真菌及哺乳动物等方面的研究。本文就蜱体内RNA干扰的机理,RNA干扰方法以及在鉴定蜱基因功能、疫苗抗原筛选以及蜱与蜱传病原体的相互作用研究上的应用进行综述分析,探讨存在的问题和进一步应用前景。  相似文献   

基于ERT技术反演红壤剖面水分入渗过程          下载免费PDF全文

田园  钟雪梅  彭新华  汪亚及  高磊 《土壤学报》2024,61(3)

水分运动是土壤中物质和能量传输的主要驱动力，但是水分在土壤中的运动轨迹复杂多变，难以直接捕捉。本研究以第四纪红黏土发育的红壤为研究对象，借助电阻率成像技术（ERT），反演水分在林地和农地土壤中的入渗过程。研究发现，ERT技术能够实现水分在红壤包气带入渗过程的可视化，ERT测定的表观电阻率的变化量与烘干法测定的土壤水分之间呈极显著正相关关系（P<0.01），林地的决定系数为0.72，农地为0.53。土地利用类型影响水分入渗的范围和方式，在2 cm水头高度注水100 L的情况下，林地的入渗深度大于80 cm，而农地约为50 cm，在此基础上，继续注水200 min，林地和农地均出现明显的优先流现象。但是，林地的优先流比农地更为发育，林地是通过根系等大孔隙快速向下传输，而农地则以“指流”的方式，间断性向深层运动。在土壤层次复杂、背景含水量高、土壤黏重等不利因素的影响下，ERT技术依然能捕捉优先流在红壤中发生和发育的过程。  相似文献   

Associations of Gonadotropin-Releasing Hormone Receptor (GnRHR) and Neuropeptide Y (NPY) Genes'''' Polymorphisms with Egg-Laying Traits in Wenchang Chicken     

WU Xu  LI Hui-fang  YAN Mei-jiao  TANG Qing-ping  CHEN Kuan-wei  WANG Jin-yu  GAO Yu-shi  TU Yun-jie  YU Ya-bo  ZHU Wen-qi 《中国农业科学(英文版)》2007,6(4):499-505

Single nucleotide polymorphisms (SNP) of chicken gonadotropin-releasing hormone receptor (GnRHR) and neuropeptide Y (NPY) were selected to identify the genotypes of Wenchang (Chinese indigenous breed) chicken with restricton fragment length polymorphisms. The associations of the SNPs with the total egg production (NE), average days of continual laying (ADCL), and number of double-yolked eggs (DYE) traits were analyzed. The frequency of restriction enzyme A/a alleles in the population was for GnRHR 0.69 (Bpu1102 Ⅰ A) and 0.31 (Bpu1102 Ⅰ a) and for NPY 0.46 (Dra Ⅰ B) and 0.54 (Dra Ⅰ b). Trait data from a total of 120 hens, which were purebred introduced from Hainan Province, China from one generation were recorded. Two significant effects of genes' marker were found for GnRHR and number of eggs (dominant; t= 2.67, df= 116) and NPY and number of eggs (additive; t= 1.97, df= 116). The current research supports the effects of GnRHR and NPY genes on egg-laying traits of chickens.  相似文献   

上市公司对外担保内部会计控制制度建设     

高晓玲 《四川畜牧兽医学院学报》2007,5(2):62-64

针对我国上市公司对外担保中出现的违规担保情况比较严重、对外担保公司自身财务状况欠佳和相互担保形成互保圈等问题，从企业内部管理角度分析问题产生的原因和对企业的危害；并从实行岗位分工与授权批准、强化担保评估机制与审批控制、严格担保业务执行控制几个方面提出加强企业对外担保内部会计控制制度建设的对策。  相似文献   

绵羊传染性脓包的防治     

郭润亚  高生英  高迪 《甘肃畜牧兽医》2010,40(2):33-34

绵羊传染性脓疮又名羊传染性脓包口炎，俗称“羊口疮”或“羊口膜炎”。是绵山羊的一种急性发疹性皮炎，成年羊多呈散发性，而羔羊多为群发性，临床特征为在口、鼻、眼睑、乳头、乳房、蹄部皮肤以及口和颊内粘膜上，依次发生丘疹、水泡、脓包和痂皮。本病常继发坏死杆菌感染。主要危害幼龄羔羊，在我县养羊地区流行比较普遍，导致了较大的经济损失。  相似文献   

测土配方施肥中应注意的几个问题   总被引：3，自引：3，他引：0  

杨政卿  高树才 《甘肃农业科技》2010,(3):46-47

总结出测土配方施肥工作中应注意的几个问题是:GPS定位要准确;基础调查要细致;选点要有代表性;样品采集要标准;土样处理要严格;试验实施要规范;样品化验要精确;数据库建立要完备。  相似文献   

Effect of Different Dietary Nutrition Levels on Fattening Effect of Holstein Bulls     

LI Xiao-meng  LI Qiu-feng  CAO Yu-feng  YU Chun-qi  LI Jian-guo  GAO Yan-xia 《中国畜牧兽医》2015,42(7):1705-1710

This experiment was conducted to study the effect of different dietary nutrition levels on fattening effect of Holstein bulls.48 4-month-old Holstein bulls with an average body weight of 128 kg were allocated into two groups,group Ⅰ was fed a low nutrition level diet,group Ⅱ was fed a high nutrition level diet.Each group had 24 Holstein bulls.The pre-test period lasted for 7 days and the trial period lasted for 245 days.The results showed that compared with group Ⅰ,the ADG in group Ⅱ was significantly increased by 6.36% (P<0.01),while the F/G in group Ⅱ was decreased by 6.55%.With the increasing of dietary nutrition levels,the apparent digestibility of CP,EE,NDF,ADF,Ca and P were rising,but there was no significant differences between two groups (P>0.05).Compared with group Ⅰ,the serum GLU,AST,ALT and GH contents in group Ⅱ were increased by 12.52%,12.85%,9.68% and 13.24% (P<0.05),respectively,while the serum BUN content in group Ⅱ was decreased by 10.98% (P<0.05).Compared with group Ⅰ,the feeding profits of group Ⅱ increased by 1.37 yuan/(head·day),the total economic benefit of group Ⅱ increased by 335.65 yuan/head.In conclusion,the improvement of dietary nutrition levels could improve the fattening performance and the economic benefit of Holstein bulls.  相似文献   

Establishment of Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry Determination Method of Amantadine in Chicken and Preparation of Positive Samples     

CHANG Ping-ping  XU Chun-nan  ZHANG Li-xin  HAO Xiao-mei  GAO Xing  CHEN Chao 《中国畜牧兽医》2015,42(9):2278-2285

An ultra performance liquid chromatography (UPLC)-tandem mass spectrometry analysis method was established in this experiment for determination of amantadine in chicken.1% trichloroacetic acid with methanol as extraction liquid, the ultrasonic assisted solvent extraction was used to extract amantadine in chicken, extract liquid was purified and enriched in MCX solid-phase extraction column.Using C18 chromatographic column as separation column, in the positive ion mode, we detected the samples by electrospray ionization tandem mass spectrometry.Mass spectrometry and chromatographic conditions, the type of extract liquid, ultrasonic extraction time, the types of solid-phase extraction column and eluent were optimized.Under optimal conditions, a good linearity (r=0.9998) in the ranges of 0.1 to 20.0 ng/mL was obtained, the recoveries were 89.7% to 101.4% in the additive levels of 5.0, 10.0 and 20.0 μg/kg, the limit of detection (LOD) of amantadine was 0.07 μg/kg, the limit of quantiation (LOQ) of amantadine was 0.23 μg/kg, and the relative standard deviations (RSD) were below 7.0%.This novel approach had high sensitivity and accuracy, and was successfully applied to rapid and high sensitive detection of amantadine residues in chicken.The positive samples of amantadine in chicken were successfully prepared with concentrations of 11.16 and 7.18 mg/kg, which could be used for the research of ELISA kits of amantadine.  相似文献   

黄河治理与排灌事业   总被引：1，自引：1，他引：0  

高良润 《排灌机械》2000,18(5):3-6

西部大开发首当其冲的是贡河治理问题。文中论述了黄河流载的黄河治理、排灌事业、防止断流、节水农业四个问题。  相似文献   

氯前列烯醇诱导母猪同步分娩的效果   总被引：2，自引：0，他引：2  

韩春梅  周虚  高庆华 《中国兽医学报》2000,20(6):605-607

选择 2～ 4胎龄施格母猪 1 32头 ,分为 2组。对照组 ( 6 5头 )不做任何处理 ,试验组 ( 6 7头 )又分妊娠 1 1 2d组 ( 34头 )、1 1 3d组 ( 33头 )。用氯前列烯醇 ( 0 .1 g/L)分 0 .5m L/头、1 m L/头 2个剂量组分别处理上述各试验组母猪 ,观察母猪的分娩情况。结果 ,处理至分娩开始的间隔 ,1 1 2 d组为 2 8.72 h,1 1 3d组为 2 3.34 h,2组差异极显著 ( P<0 .0 1 ) ;分娩产程 ,1 1 2 d组为 3.0 8h比对照组长 4 .2 min,1 1 3d组为 2 .31 h比对照组短4 2 min,无显著性差异 ( P>0 .0 5) ;而白天分娩率经χ2 检验有显著性差异 ,1 1 2 d、1 1 3d组分别有 6 1 .76 %和78.78%的母猪在白天分娩 ,而对照组仅为 4 6 .1 5% ( P<0 .0 5)。1 1 2 d组、1 1 3d组母猪的生产成绩 ,死胎率分别为 2 .2 7%和 2 .0 8%比对照组低 1 .82 %、2 .0 1 % ( P<0 .0 5) ;初生窝重分别为 1 1 .56、1 0 .55kg,分别比对照组高 0 .0 8kg和低 0 .0 7kg( P>0 .0 5) ;2 0日龄窝重 ,1 1 2 d组平均为 33.56 kg比对照组低 1 .9kg,1 1 3d组为2 9.30 kg比对照组高 1 .77kg( P>0 .0 5) ;断奶个体重 ,1 1 2 d组平均为 8.1 4 kg比对照组低 0 .2 7kg,1 1 3d组平均为 9.2 7kg比对照组高 0 .85kg( P>0 .0 5)。结果表明 ,氯前列烯醇能有效控制母猪分娩时间集  相似文献