禽呼肠孤病毒C-98分离株L1基因的克隆与序列分析   总被引：2，自引：2，他引：0  

郝瑞峰  关平原  乌日罕  特木尔巴根  马立峰  于富丽  李瑞纲 《中国畜牧兽医》2008,35(9):37-40

参考禽呼肠孤病毒S1133株（GenBank）L1基因序列设计3对特异性引物,采用RT-PCR方法对禽呼肠孤病毒内蒙古分离株C-98的L1基因进行了克隆和序列测定。结果表明：获得的C-98 L1基因全长3959 bp,其中包括21 bp的5’非编码区和56 bp的3’非编码区,阅读框位于5’端第22位核苷酸与3’端第3903位核苷酸之间。将C-98株与国内外禽呼肠孤病毒群和哺乳动物呼肠孤病毒群的不同分离株进行序列比较,结果显示：C-98与台湾地区分离株919和美国分离株1733、2408、S1133亲缘关系最近,其核苷酸同源性分别为99.8%、99.7%、99.7%、99.4%;与哺乳动物呼肠孤病毒L3基因核苷酸及其编码的氨基酸同源性较低,为43%～45%。  相似文献   

黄瓜幼苗光合及荧光特性对弱光的响应   总被引：2，自引：0，他引：2  

李伟  黄金丽  眭晓蕾  王绍辉  关秋竹  周明  胡丽萍  张振贤 《园艺学报》2008,35(1):119-123

 以不耐弱光的‘津研2号’和较耐弱光的‘戴多星’黄瓜(Cucumis sativus L.)为试材,在人工气候室内研究其光合及荧光特性对弱光（75～85 μmol·m^-2·s^-1）的响应。结果表明,弱光处理后叶片光合色素含量、总叶绿素/类胡萝卜素比值呈增加趋势,尤其是戴多星更明显;叶绿素a/b比值呈减小趋势,且戴多星的减小幅度大于津研2号;净光合速率、水分利用效率呈下降趋势,戴多星下降幅度小于津研2号;叶片最大光化学效率、光系统Ⅱ实际光化学效率、光化学反应速率、光系统非环式电子传递速率、光系统Ⅱ吸收光能用于光化学反应的比例,对弱光的响应有一定差异,弱光下戴多星均有明显增加,而津研2号的增减不规律,且幅度均较小,说明弱光下戴多星对弱光的利用能力明显强于津研2号。  相似文献   

Improved biological effects of biomimetic nanoapatite coatings of titanium surface on osteoblasts-like viability     

GUAN Bing-gang  YANG Di-sheng  YAN Wei-qi 《园艺学报》2008,24(3):532-536

AIM: To investigate influence of biomimetic nanoapatite coatings of titanium surface on behavior of osteoblasts-like and provide evidence for surface modification and biological effects of titanium implant. METHODS: Biomimetic nanoapatite coatings were developed by functionally modified methods with a combination of topographic, chemical and biomimetic treatments on the surface of titanium (Ti) substrate. The biological behavior and bioactivity of functionally modified SLA implants with chemical and biomimetic treatments (SCB-treated Ti) were investigated to compare with untreated Ti and SLA Ti plates as controls. The cell attachment, proliferation, alkaline phosphotase (ALP) activity, cell morphology and differentiation were evaluated by using MTT, RT-PCR, scanning electron microscopy (SEM) and confocal laser-scanning microscope (CLSM) analysis system. RESULTS: The cell adhesion and proliferation were enhanced on functionalized titanium surface with nano-scale apatite compared to the controls. SEM micrographs also revealed that the osteoblast-like cells spreadly grew along the surface. Cell morphology and differentiation were further observed distinctly by CLSM graphs. Moreover, mRNA expression of alkaline phosphotase on the SCB-treated Ti increased obviously on the twelfth day compared with the controls. CONCLUSION: The in vitro results demonstrate the remarkable improvement on cell adhesion and proliferation of the biomimetic nanoapatite on SCB-treated Ti, which could improve early bone-implant interface bonding ability and be used for orthopaedic/dental implants.  相似文献   

毛百合×有斑百合种间杂种的育成   总被引：1，自引：0，他引：1  

关婧竹  雷家军 《北方园艺》2008,(4):155-157

以毛百合(Lilium dauricum)为母本、有斑百合(L.concolor var.prlchellum)为父本进行常规杂交,通过胚培养成功获得了种间远缘杂交种,对种问杂交亲和性、胚培养和杂种的形态特征进行了调查研究.结果表明:毛百合×有斑百合蒴果膨大率达46.1%,种子有胚率为1.30%,胚培养萌发率为15.2%;4个杂交后代生长旺盛,株高表现出较明显的超亲现象,其他性状呈趋中趋势,杂种植株的花色更偏向橙色.4个杂交后代虽然在花色上与亲本有一定差异,但其它性状均具有双亲的特征,从形态上能证明是真杂种.  相似文献   

京郊有机苹果园茶翅蝽发生规律及控制策略     

蔡乐  董民  杜相革 《北方园艺》2008,(11)

在有机管理方式下,茶翅蝽Halyomorpha halys (St(.a)l),已上升为苹果园主要害虫,成为防治的重要难题之一.2007年在北京昌平地区有机苹果园中,通过枝干调查、杀虫灯诱集对茶翅蝽的发生情况进行调查,明确其发生及迁移规律,为茶翅蝽的控制提供依据.在控制策略上,以重点消灭第一代成虫为主,灵活运用各种植保措施.  相似文献   

豇豆新品种贺研1号的选育   总被引：1，自引：1，他引：0  

杨连勇  杨孚初  管锋  姜守全  谭旭生  王莉 《长江蔬菜》2008,(16)

贺研1号是从常德收集到的农家资源贺H-18中选择优良变异株经系统选育而成的极早熟豇豆新品种.春季栽培,全生育期80～90 d,播种至始花40～45 d,播种至始收50～55 d;夏季栽培,全生育期70～80 d,播种至始花30～35 d,播种至始收40～45 d.豆荚淡绿色,荚长约71 cm,荚横径0.88～0.93 cm,单荚质量25.8～27.4 g,荚条匀称,商品性好,鲜食、腌制皆宜.抗病性中等,前期产量18 t·hm-2左右,总产量33～36 t·hm-2.是适于城郊蔬菜种植区作春提早鲜荚上市栽培的专用品种.  相似文献   

广州地区草菇产业现状及发展对策     

彭增明  蔡月彩  曹学文  曹湛才  曾志忠  关雁桃 《长江蔬菜》2008,(12)

根据广州地区草菇产业发展现状,找出广州地区草菇业发展存在的问题,并提出相应的对策.主要包括:发展广州地区草菇产业,需要扩大宣传、提高产品质量、实施标准化品牌战略、加强产业相关部门的合作等.  相似文献   

食用菌药残留限量与产品质量安全   总被引：11，自引：0，他引：11  

管道平  胡清秀 《中国食用菌》2008,27(2):3-6

分析了食用菌生产使用农药情况和国内外食用菌农药残留限量标准状况,从食用菌生产的产地环境、食用菌菇房建筑标准、农药使用规范以及食用菌安全生产全程质量控制等方面提出了应对措施,为食用菌安全生产和全面提高食用菌产品质量提供参考。  相似文献   

IL-9 induces epithelial-mesenchymal transition of gastric cancer MKN-45 cells by TGF-β/Smad signaling pathway     

RUAN Shui-liang  HAN Chen-yang  GUAN Qiao-bin  YANG Yi 《园艺学报》2018,34(10):1748-1753

  相似文献   

Effects of Ku70 on protein expression of HTLV-1 in HTLV-1 positive T cells     

SONG Di  MA Ling-ling  GUO Zhi-xiang  LIU Yue  CUI Yu-han  GUAN Yu-he  YANG Bo  WANG Jie 《园艺学报》2018,34(12):2261-2266

AIM:To explore the effects of Ku70 on the protein expression of human T-lymphotrophic virus 1 (HTLV-1) in HTLV-1 positive T cells. METHODS:The expression level of Ku70 in HTLV-1 positive T cells was exa-mined by Western blot. The siRNA targeting Ku70 was constructed and the effect of the siRNA on knockdown of Ku70 expression was determined by Western blot. After knockdown of Ku70 expression in the HTLV-1 positive T cells by siRNA, the expression of HTLV-1-related proteins at mRNA and protein levels was examined by real-time PCR and Western blot, and the expression levels of interferons and proinflammatory cytokines were examined by real-time PCR. RESULTS:The HTLV-1 positive T cells, including MT2, MT4 and C8199 cells, displayed a higher expression level of Ku70. The protein expression of HTLV-1 was increased in Ku70-silencing MT2 cells and MT4 cells. After knockdown of Ku70 expression in the MT2 cells and MT4 cells, the production of interferon (IFN)-α, IFN-γ and tumor necrosis factor-α was reduced.CONCLUSION:The HTLV-1 positive T cells have a higher expression level of Ku70. In HTLV-1 positive T cells, Ku70 promotes the production of interferons and proinflammatory cytokines and inhibits HTLV-1-related protein expression.  相似文献