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41.
Abstract –  Pseudoplatystoma corruscans is distributed widely in the La Plata Basin in Brazil, where it is a very important species because of its high commercial value and ecological role as a voracious predator. This species undertakes long migrations during the dry and rainy seasons to feed and reproduce, respectively. In this study, we analysed seven microsatellite loci to test the hypotheses of the existence of a single panmictic population of P. corruscans in the La Plata Basin. All microsatellites analysed were highly polymorphic with the number of alleles per locus ranging from seven (Pcor28) to 30 (Pcor10). Across all samples, 107 alleles were detected. Thirty alleles were private, i.e., found in only one sample. Highly significant genetic differentiation was observed among samples in three of the four analyses performed: F ST (from 0.03435 to 0.16349, P  < 0.05), Analysis of molecular variance (amova) F ST = 8.25% ( P  < 0.0001) and amova R ST = 9.72% ( P  < 0.0001). A microsatellite multilocus Bayesian assignment test with the programme structure confirmed the division of the fishes into six groups largely concurrent with main branches on a population neighbour-joining tree. The obtained results rejected the panmixia hypothesis. On the other hand, the documented long-distance movements of P. corruscans and the geographical scale of genetic differentiation found in this study indicate a strong tendency for fish of this species to utilise their natal nursery regions for reproduction, which represents the first demonstrated example of homing in a freshwater catfish in South America.  相似文献   
42.
The Central Asian countries face high water scarcity due to aridity and desertification but excess water is often applied to the main irrigated crops. This over-irrigation contributes to aggravate water scarcity problems. Improved water saving irrigation is therefore required, mainly through appropriate irrigation scheduling. To provide for it, after being previously calibrated and validated for cotton in the Fergana region, the irrigation scheduling simulation model ISAREG was explored to simulate improved irrigation scheduling alternatives. Results show that using the present irrigation scheduling a large part of the applied water, averaging 20%, percolates out of the root zone. Several irrigation strategies were analyzed, including full irrigation and various levels of deficit irrigation. The analysis focused a three-year period when experiments for calibration and validation of the model were carried out, and a longer period of 33 years that provided for an analysis considering the probabilities of the demand for irrigation water. The first concerned a wet period while the second includes a variety of climatic demand conditions that provided for analyzing alternative schedules for average, high and very high climatic demand. Results have shown the importance of the groundwater contribution, mainly when deficit irrigation is applied. Analyzing several deficit irrigation strategies through the respective potential water saving, relative yield losses, water productivity and economic water productivity, it could be concluded that relative mild deficits may be adopted. Contrarily, the adoption of high water deficit that produce high water savings would lead to yield losses that may be economically not acceptable.  相似文献   
43.
Veterinary Research Communications - This study aimed to describe the transmission of T. gondii in naturally infected goats in the semiarid region of northeastern Brazil, through evaluating the...  相似文献   
44.
Crude extracts and fractions of Bertholletia excelsa stem barks were tested for trypanocidal activity. Acetone and methanol extracts showed significant in vitro trypanocidal activity against trypomastigote form of Trypanosoma cruzi since in the concentration of 500 microg/ml, the parasites were reduced in 100% and 90.3% respectively, whereas the triterpene betulinic acid pure isolated from hexane extract presented 75.4%.  相似文献   
45.
Pinus halepensis has been described as a drought-tolerant species with high plasticity to growth in different environments. Its eco-physiological characteristics could facilitate the use of this species in large afforestations in the future scenery of climate change. Somatic embryogenesis is a biotechnological tool with potential for large-scale clonal propagation. In order to establish an improved regeneration protocol for Pinus halepensis, the effects of different temperatures (18, 23, and 28 °C) and water availability conditions (2, 3, and 4 g L?1 Gelrite®), during initiation of embryonal masses on the rate of initiation, proliferation, maturation, and the number of embryos developed, were evaluated. It was found that environmental conditions during the initiation stage of Pinus halepensis somatic embryogenesis influence the success of initiation and proliferation. In contrast, there was no effect of these conditions on the maturation rates and the number of somatic embryos. Somatic embryos were obtained in all treatments tested, indicating that plants can be produced from extreme conditions of induction, such as high temperatures (28 °C) and low water availability conditions (4 g L?1).  相似文献   
46.
Mixed tree cropping systems have been proposed for sustainable nutrient management in the humid tropics. Yet, the nutrient interactions between intercropped trees have not been addressed sufficiently. In the present study we compare the temporal and spatial patterns of the uptake of applied 15N by four different tree crops in a mixed tree cropping system on a Xanthic Ferralsol in central Amazônia, Brazil, during one year. Most of the N uptake occurred during the first two weeks. Very little N was recovered by peach palm (Bactris gasipaes), more by cupuassu (Theobroma grandiflorum) and annatto (Bixa orellana) and most by Brazil nut (Bertholletia excelsa). Due to tree pruning the total accumulation of applied 15N in the above-ground biomass of annatto decreased throughout the year. It remained constant in cupuassu and peach palm and increased in Brazil nut. Brazil nut showed an extensive root activity and took up more fertilizer N applied to neighboring trees than from the one applied under its own canopy in contrast to the other three tree crops. Therefore, trees with wide-spread root systems may not need to receive N fertilizer directly but can take up N applied to other trees in the mixed cropping system. This means that such trees may effectively decrease N leaching when intercropped with trees that have dormant periods or places with low N uptake, but also exert considerable resource competition.This revised version was published online in November 2005 with corrections to the Cover Date.  相似文献   
47.
The antifungal activity of Eugenia cariophyllata essential oil and eugenol, its major constituent, on fungal strains isolated from onychomycosis was evaluated. The natural products presented prominent antifungal action with MIC of 1% and 4%, respectively.  相似文献   
48.
Summary The chemical composition of cork from Quercus suber L. was determined for virgin cork and for reproduction cork. Average chemical composition of virgin cork is: ash 0.7%, total extractives 15.3%, suberin 38.6%, lignin 21.7% and polysaccharides 18.2%. The carbohydrate composition shows that glucose represents 50.6% of all monosaccharides, xylose 35.0%, arabinose 7.0% and galactose and mannose, respectively, 3.6% and 3.4%. Reproduction cork has a composition similar to virgin cork. For the cases studied, reproduction cork had however less extractives and a higher amount of suberin. Significant differences were found for the chemical composition of virgin cork in relation to location of extractives and polysaccharide content. The between-tree variability was large and a variation within the tree was also recorded.The author is grateful for the help of collaborators throughout the chemical analysis of the many samples: Joaquina Ferreira, A. Velez Marques, M. Lurdes Ferreira, Rosa Amorim and Célia Constâncio.The work was financially supported by the Instituto de Ciência e Tecnologia dos Materiais (ICTM), Lisbon, Portugal  相似文献   
49.
Quercus suber L. is an important species producing cork whose wood characteristics have not been investigated a lot. Cork oak wood vessels are a striking feature and the most abundant wood tissue largely influencing density and permeability. Vessel size and distribution were studied in approximately 40 year-old and never debarked cork oaks by continuously measuring along the radial direction in the transverse section of wood discs taken at 1.3 m of height using image analysis techniques. The vessel size increases with age from 7660 ± 2286 to 21136 ± 6119 μm2, the conductive area from 5.4 ± 2.2 to 11.6 ± 3.9%, and the vessel density remains approximately constant between 5.2 ± 1.5 and 7.3 ± 3.5 vessels/mm2. In comparison with ring-porous and some evergreen oaks, cork oaks show a similar conductive area but smaller vessels. Vessel architecture is known to play an important role on oaks tolerance to hydric stress, and these cork oak trees were growing under very harsh edaphoclimatic conditions, not tolerated by other oaks. The well-developed and deep root system allowing access to constant water supply may contribute to the cork oak’s relatively high conductive area.  相似文献   
50.
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