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51.
OBJECTIVE: To report outcomes after reconstruction of the lower eyelid following resection of squamous cell carcinoma (SCC). STUDY DESIGN: Retrospective study. ANIMALS: Five cats (6 eyelids). METHODS: Case records were reviewed for breed, sex, surgical findings, complications, and outcome. RESULTS: Six eyelids were reconstructed in 5 cats; functional outcome was excellent. Neither wound breakdown nor flap failure occurred. An area of superficial necrosis occurred in 1 cat; this spared the eyelid margin and healed by secondary intention without any cosmetic or functional effect. One cat was euthanatized 18 months later because of a nasal mass. The relationship between the nasal mass and the previously excised SCC was not established. CONCLUSIONS: Reconstruction of the lower eyelid using a lip-to-lid flap yields satisfactory functional and cosmetic results in cats. CLINICAL RELEVANCE: A lip to lid flap is a successful form of single-stage reconstruction after lower eyelid excision in cats.  相似文献   
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Diffusion-ordered spectroscopy (DOSY)-pulsed field gradient (PGF) nuclear magnetic resonance (NMR) spectroscopy was used to measure self-diffusion coefficients of aroma molecules in model fruit preparations. The impact of the sucrose content on aroma diffusion was specifically investigated, and the relationship with viscosity, water activity, and dry matter parameters was evidenced. DOSY-PGF NMR spectroscopy was found to be a relevant and accurate technique to follow self-diffusion of aroma compounds at low concentrations in a complex food matrix and to obtain information on diffusion of the sucrose and of the water molecules. We showed that aroma self-diffusion was strongly decreased in fruit preparation because of the high sucrose content, which induces the formation of a network through hydrogen bonds with water. Self-diffusion coefficients were determined for aroma molecules of different natures, and values are related to the physicochemical properties of the molecule.  相似文献   
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Temperature-induced alterations in the activity of the enzyme lactate dehydrogenase (LDH) were evaluated in the freshwater prawn Macrobrachium malcolmsonii in three groups of juveniles: controls maintained at 27±2 °C; test prawns exposed to 35 °C; test prawns exposed to 15 °C. Changes in LDH activity and lactate levels in key tissues were assessed after 48 hrs. LDH in the skeletal muscle of the prawns was also subjected to kinetic analysis at different temperatures. Native polyacrylamide gel electrophoresis (PAGE) analysis and colorimetric estimation revealed decreased LDH activity (compared to controls) in the gill, heart and haemolymph, but not in the skeletal muscle or hepatopancreas, of test prawns exposed to 15 °C; however, lactate levels were significantly lower in all the tissues of these test prawns. Conversely, prawns exposed to 35 °C revealed elevated LDH activity in all the tissues, barring the skeletal muscle, while lactate levels were significantly higher (compared to controls) in all the tissues of these prawns. Kinetic analysis of LDH in the skeletal muscle at different assay temperatures revealed temperature-dependent kinetic properties. The differences observed in LDH activity and levels of lactate in various tissues of prawns exposed to low and high temperatures suggest aerobic and anaerobic patterns of pyruvate metabolism at respective temperatures. The results obtained by kinetic analysis of LDH in the skeletal muscle suggest the occurrence of an adaptative response involving this enzyme that enables M. malcolmsonii to cope with effects of thermal stress. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   
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Asymmetric segregation of P granules during the first four divisions of the Caenorhabditis elegans embryo is a classic example of cytoplasmic partitioning of germline determinants. It is thought that asymmetric partitioning of P granule components during mitosis is essential to distinguish germline from soma. We have identified a mutant (pptr-1) in which P granules become unstable during mitosis and P granule proteins and RNAs are distributed equally to somatic and germline blastomeres. Despite symmetric partitioning of P granule components, pptr-1 mutants segregate a germline that uniquely expresses P granules during postembryonic development. pptr-1 mutants are fertile, except at high temperatures. Hence, asymmetric partitioning of maternal P granules is not essential to specify germ cell fate. Instead, it may serve to protect the nascent germline from stress.  相似文献   
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Wetland creation aiming at a simultaneous increase in nutrient retention and species diversity in agricultural landscapes has recently become applied as a catchment-scale compensation measure for past wetland losses. Here, we evaluate if, and to what extend, dual-purpose wetlands benefit local and regional diversity of agricultural landscapes. We analysed composition and α, β, and γ diversity of aquatic macroinvertebrate assemblages among dual-purpose wetlands in an agricultural region in southwest Sweden in relation to local (water quality, wetland morphology, succession stage, proximity to other aquatic habitats) and landscape parameters (regional connectivity, wetland density). Diversity of mature agricultural ponds was used as a standard to evaluate the value of dual-purpose wetlands. Dual-purpose wetlands sustained α, β, and γ diversity similar to that of natural lentic water bodies in agricultural landscapes in the region and elsewhere. Over 80% of the overall species richness was attributed to β diversity, and each created wetland contributed to overall species accumulation. Ecosystem parameters explained 19% of the compositional variation among assemblages, but were only marginally related to diversity. Wetland density promoted α and γ diversity, while spatial heterogeneity (β) remained equally high, independent of wetland density. Our results indicate that catchment-scale wetland creation for simultaneous retention and diversity purposes benefits the biodiversity of agricultural landscapes, particularly if the density of aquatic habitats is increased by at least 30%.  相似文献   
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