Dynamic patterns of components of genotype × environment interaction for pod yield of peanut over multiple years: A simulation approach     

N. Phakamas  A. Patanothai  K. Pannangpetch  S. Jogloy  Gerrit Hoogenboom 《Field Crops Research》2008

The relative importance of the genotype × year (G × Y), genotype × location (G × L) and genotype × location × year (G × L × Y) interactions has significant implications on the testing strategy of crop breeding lines. The goal of this study was to examine the dynamic patterns of these three interactions for pod yield of peanut using a crop simulation model. Pod yields of 17 peanut lines in the early-rainy, mid-rainy and dry seasons at 112 locations covering all peanut production areas in Thailand were simulated for 30 years (1972–2002) with the Cropping System Model (CSM)-CROPGRO-Peanut. Combined analyses of variance were preformed for individual seasons and for overall seasons, with the number of year incrementally increasing from 2 to 30, and the relative contributions of the individual sources of variation were determined. This procedure was repeated four times with different starting years. The results showed that the environmental effects accounted for the major proportion of the total yield variation, followed by the genotype effects, while the genotype × environment (G × E) effects were rather small. The contributions of the individual sources changed as the number of years in the analysis changed. Increasing number of years in the analyses resulted in an increase in the magnitude of the G × Y and G × L × Y interactions, but a decline in the G × L contribution. The contributions of the G × Y and G × L interactions were greater and more fluctuated in the dry season, while those of the G × L × Y interactions were greater in the mid-rainy season. Notable increases in the G × Y interaction in the dry season were observed in certain years. The decline in the G × L interaction with increasing number of years was closely associated with the increase in the G × L × Y interaction, and both became stable when 6 or more years were included. Several cross-over in the ranks of peanut lines for mean pod yield in two contrasting years were also observed for the mid-rainy season. These results raise a question on the effectiveness of the strategy for using locations to replace years in varietal testing that is normally employed by breeders. The practical limit of multi-year evaluation of crop breeding lines could be overcome by the use of a crop simulation model.  相似文献   

Growing food,growing a movement: climate adaptation and civic agriculture in the southeastern United States     

Carrie Furman  Carla Roncoli  Donald R. Nelson  Gerrit Hoogenboom 《Agriculture and Human Values》2014,31(1):69-82

This article examines the role that civic agriculture in Georgia (US) plays in shaping attitudes, strategies, and relationships that foster both sustainability and adaptation to a changing climate. Civic agriculture is a social movement that attracts a specific type of “activist” farmer, who is linked to a strong social network that includes other farmers and consumers. Positioning farmers’ practices within a social movement broadens the understanding of adaptive capacity beyond how farmers adapt to understand why they do so. By drawing upon qualitative and quantitative data and by focusing on the cosmological, organizational, and technical dimensions of the social movement, the study illuminates how social values and networks shape production and marketing strategies that enable farmers to share resources and risks. We propose a conceptual framework for understanding how technical and social strategies aimed to address the sustainability goals of the movement also increase adaptive capacity at multiple timescales. In conclusion, we outline directions for future research, including the need for longitudinal studies that focus on consumer motivation and willingness to pay, the effects of scale on consumer loyalty and producer cooperation, and the role of a social movement in climate change adaptation. Finally, we stress that farmers’ ability to thrive in uncertain climate futures calls for transformative approaches to sustainable agriculture that support the development of strong social networks.  相似文献   

Cortisol-binding globulin and meat quality in five European lines of pigs     

Geverink NA  Foury A  Plastow GS  Gil M  Gispert M  Hortós M  Font i Furnols M  Gort G  Moisan MP  Mormède P 《Journal of animal science》2006,84(1):204-211

The gene (Cbg) encoding cortisol-binding globulin (CBG) has been proposed as a candidate gene to explain genetic variation in cortisol secretion and carcass composition in pigs. The objective of this study was to evaluate the association between CBG and pork quality in 5 European breeding lines, Piétrain, Large White (LW), and Landrace purebred lines, a Duroc synthetic line, and a Meishan (MS) x LW advanced intercross. Cortisol-binding globulin maximum binding capacity (CBG-Bmax) was twice as high (P < 0.05) in MS x LW pigs compared with the other lines. There was no (P > or = 0.364) association between CBG-Bmax and carcass quality traits in Piétrain gilts, but CBG-Bmax was associated with increased loin yields in LW (P = 0.010) and Landrace (P = 0.103) gilts, decreased ham yields (P = 0.082) in Duroc gilts, and increased fat depth (P = 0.064) and leaf fat (P = 0.001) in MS x LW gilts. There was no association between CBG-Bmax and pork quality traits in Piétrain (P > or = 0.269) and Duroc (P > or = 0.114) gilts. Conversely, CBG-Bmax was associated with lighter (higher L* values; P < 0.05) pork in Land-race gilts, as well as lower (P < or = 0.055) ultimate pH in the LM and semimembranosus, and a tendency for lower (P = 0.095) L* values of pork from LW gilts. Within MS x LW pigs, CBG-Bmax was associated with increased drip loss (P = 0.001) and decreased i.m. fat in the semimembranosus (P = 0.005). Because drip loss is an economically important pork quality trait, results of this study could be used in the selection of improved water-holding capacity of pork from synthetic lines involving the MS breed.  相似文献   

Optimal dose and timing in phytohaemagglutinin skin-testing of deer     

Fernández-de-Mera IG  Höfle U  Vicente J  Garcia A  Rodriguez O  Gortázar C 《New Zealand veterinary journal》2006,54(6):357-359

AIM: To establish the optimal dose of the mitogen phytohaemagglutinin (PHA) and the optimal time for measuring increased skin-fold thickness in red deer following intradermal injection, as an indicator of cell-mediated immune response. METHODS: Three doses (10, 50 and 250 microg) of PHA were injected intradermally in the right side of the neck, and phosphate buffered saline (PBS) was injected at a fourth site as a control, in 20 captive Iberian red deer (Cervus elaphus hispanicus) hinds. Skin-fold thicknesses were measured at 0, 12, 24, 36, 48, 60, 72, 84 and 96 h following injection. RESULTS: The highest dose of PHA tested (250 microg) resulted in a clear and long-lasting cellular response; increases in skin-fold thickness between 48 and 84 h post-injection varied minimally and response correlated positively with liveweight. No correlations with liveweight and no clear increases in skin-fold thickness occurred at the lower doses of PHA or the PBS. CONCLUSIONS AND CLINICAL RELEVANCE: This technique could be applied with minimal training and without specialised equipment in deer, for immunological and ecological research.  相似文献   

Review and simulation of homoplasy and collision in AFLP     

Gerrit Gort  Fred A. van Eeuwijk 《Euphytica》2012,183(3):389-400

In this paper we give a short review of the problems of homoplasy and collision in AFLP, and describe a software tool that we developed to illustrate these problems. AFLP is a DNA fingerprinting technique, producing profiles of bands, the result of the separation of DNA fragments by length on a gel or microcapillary system. The profiles are usually interpreted as binary band absence/presence patterns. We focus on two major problems: (1) Within a profile two or more fragments of the same length but of different genomic origin may have been selected, colliding into a single band. This collision problem, akin to the birthday problem, may be surprisingly large. (2) In a pair of profiles two equally long fragments of different genomic origin may have been selected, appearing as identical bands in the two profiles. This is called homoplasy. Both problems are quantified by modeling AFLP as a random sampling technique of fragment lengths. AFLP may be used in phylogenetic studies to estimate the pairwise genetic similarity of individuals. Similarity coefficients like Dice and Jaccard coefficients overestimate the true genetic similarity because of homoplasy, with increasing bias for higher numbers of bands per profile. Corrected estimators are described, which do not suffer from bias. The ideas are illustrated using a new software tool. Data from studies on Arabidopsis and tomato serve as examples. Finally, we make some recommendations with respect to the use of AFLP.  相似文献   

Flavor release and perception of flavored whey protein gels: perception is determined by texture rather than by release     

Weel KG  Boelrijk AE  Alting AC  Van Mil PJ  Burger JJ  Gruppen H  Voragen AG  Smit G 《Journal of agricultural and food chemistry》2002,50(18):5149-5155

Five whey protein gels, with different gel hardnesses and waterholding capacities, were flavored with ethylbutyrate or diacetyl and evaluated by a 10-person panel to study the relation between the gel structure and the sensory perception, as well as the nosespace flavor concentration during eating. The sensory perception of the flavor compounds was measured by the time-intensity method, while simultaneously the nosespace flavor concentration was monitored by the MS-Nose. The nosespace flavor concentration was found to be independent of the gel hardness or waterholding capacity. However, significant changes in flavor intensity between the gels were perceived by the majority of the panelists, despite the fact that the panelists were instructed to focus only on flavor perception and to not take texture into account. From these observations it is concluded that the texture of gels determines perception of flavor intensity rather than the in-nose flavor concentration.  相似文献   

Sugar yield,nitrogen uptake by sugar beet and optimal nitrogen fertilization in relation to nitrogen soil analyses and several additional factors     

Victor J. G. Houba  Antoon W. M. Huijbregts  Peter Wilting  Ivo Novozamsky  Gerrit Gort 《Biology and Fertility of Soils》1995,19(1):55-59

To test the relative usefulness of different methods of chemical analysis for soil nitrogen fractions in the assessment of the fertilizer nitrogen needs of sugar beet, different doses of nitrogen were applied in field experiments laid out during the years 1985–1991. The chemical methods used were N mineral (NO _– ³ +NH ₊ ⁴ ) analyses on soil samples taken in late winter, and extraction with 0.01 M CaCl₂ from soil samples taken the preceding autumn and in late winter. The results of the chemical methods were evaluated in models using estimated optimum nitrogen fertilization, nitrogen present in beets or beets+leaves at leaf maximum and sugar yield as variables. In addition, parameters such as estimates of possible rooting depth and mineralization capacity of the soil were also included in the model. All models for estimating nitrogen fertilization need showed low R ² values. The two methods of soil chemical analysis yielded similar R ² values for nitrogen uptake in plots both with and without nitrogen fertilization. The N mineral method was least useful in predicting sugar yield. Addition of the covariables rooting depth and mineralization capacity appreciably improved the explanatory value of the models with 0.01 M CaCl₂, especially when the analytical results of soil samples taken in autumn were used. For the N mineral method the addition of covariates was found to have far less influence.  相似文献   

Structural characterization of potato protease inhibitor I (Cv. Bintje) after expression in Pichia pastoris     

van den Broek LA  Pouvreau L  Lommerse G  Schipper B  Van Koningsveld GA  Gruppen H 《Journal of agricultural and food chemistry》2004,52(15):4928-4934

In the present study the structural properties of potato protease inhibitor 1 (PI-1) were studied as a function of temperature to elucidate its precipitation mechanism upon heating. A cDNA coding for PI-1 from cv. Bintje was cloned and expressed in Pichia pastoris. Using the recombinant PI-1 it was suggested that PI-1 behaves as a hexameric protein rather than as a pentamer, as previously proposed in the literature. The recombinant protein seems either to have a predominantly unordered structure or to belong to the beta-II proteins. Differential scanning calorimetry analysis of PI-1 revealed that its thermal unfolding occurs via one endothermic transition in which the hexameric PI-1 probably unfolds, having a dimer instead of a monomer as cooperative unit. The transition temperature for the recombinant PI-1 was 88 degrees C. Similar results were obtained for a partially purified pool of native PI-1 from cv. Bintje.  相似文献   

Formation and stability of foams made with sunflower (Helianthus annuus) proteins     

González-Pérez S  Vereijken JM  van Koningsveld GA  Gruppen H  Voragen AG 《Journal of agricultural and food chemistry》2005,53(16):6469-6476

Foam properties of a sunflower isolate (SI), as well as those of helianthinin and sunflower albumins (SFAs), were studied at various pH values and ionic strengths and after heat treatment. Less foam could be formed from helianthinin than from SFAs, but foam prepared with helianthinin was more stable against Ostwald ripening and drainage than foam prepared with SFAs. Foams made with SFAs suffered from extensive coalescence. The formation and stability of foams made from reconstituted mixtures of both proteins and from SI showed the deteriorating effect of SFAs on foam stability. Foam stability against Ostwald ripening increased after acid and heat treatment of helianthinin. Partial unfolding of sunflower proteins, resulting in increased structural flexibility, improved protein performance at the air/water interface. Furthermore, it was observed that the protein available is used inefficiently and that typically only approximately 20% of the protein present is incorporated in the foam.  相似文献   

Structure and stability of the potato cysteine protease inhibitor group (cv. Elkana)     

Pouvreau L  Kroef T  Gruppen H  van Koningsveld G  van den Broek LA  Voragen AG 《Journal of agricultural and food chemistry》2005,53(14):5739-5746

The conformational stability of potato cysteine protease inhibitor (PCPI), the second most abundant protease inhibitor group in potato tuber, was investigated at ambient temperature and upon heating using far- and near-UV circular dichroism spectroscopy, fluorescence spectroscopy, and differential scanning calorimetry (DSC). The PCPI isoforms investigated have a highly similar structure at both the secondary and the tertiary level. PCPI isoforms show structural properties similar to those of the potato serine protease inhibitor group and the Kunitz type soybean trypsin inhibitor, a known beta-II protein. Therefore, PCPI isoforms are also classified as members of the beta-II protein subclass. Results show that the thermal unfolding of PCPI isoforms does not follow a two-state mechanism and that at least one intermediate is present. The occurrence of this intermediate is most apparent in the thermal unfolding of PCPI 8.3 as indicated by the presence of two peaks in the DSC thermogram. Additionally, the formation of aggregates (>100 kDa), especially at low scan rates, increases the apparent cooperativity of the unfolding.  相似文献