Effects of Foliar Application of FeSO4 and NaCl Salinity on Vegetative Growth,Antioxidant Enzymes Activity,and Malondialdehyde Content of Tanacetum balsamita L.     

Rana Valizadeh Kamran  Lamia Vojodi Mehrabani  Mohammad Bagher Hassanpouraghdam 《Communications in Soil Science and Plant Analysis》2017,48(16):1852-1859

Salinity is one of the major environmental stressors which has deleterious effects on the growth, development, and yield of crops. Because of the gradual increase in soil and water salinity in the East Azarbaijan, Iran, Tanacetum balsamita L. cultivation in this region has always been associated with many problems. To study the effect of foliar spray of iron sulfate (FeSO₄) (0, 750, and 1500 mg L^?1) under sodium chloride (NaCl) salinity (0, 50, and 100 mM) on some physiological characteristics of Tanacetum balsamita L. plants, an experiment was conducted as a factorial based on complete randomized block design with three replications. Total soluble solids (TSS) and essential oil contents were significantly affected by the interaction effects of FeSO₄ foliar application and salinity levels. The highest TSS and essential oil content were found in the plants under NaCl₀ × FeSO₄ 1500 mg L^?1 treatment combination. Leaf length, leaf fresh and dry weights were influenced by both Fe foliar application and salinity levels. Foliar application of iron (Fe) positively affected leaf length, leaves fresh and dry weights, root fresh and dry weights and peroxidase (POD) content, especially at ₁₅₀₀ mg L^?1. Other traits such as leaf length, leaf fresh and dry weights, malondialdehyde (MDA), POD and catalase (CAT) contents were influenced by salinity levels. For POD, MDA, and CAT contents, the highest values were recorded with NaCl 50 and 100. The highest values of leaf length, leaf fresh and dry weights were found in the control plants.  相似文献   

Efficiency of SCNT Buffalo (Bubalus bubalis) Embryos in Different Culture Medium and Analysis of mRNA Expression of Insulin‐like Growth Factors During Embryogenesis     

A Pandey  SC Gupta  N Singh  JS Rana  N Gupta 《Reproduction in domestic animals》2010,45(5):786-795

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Effect of the Biocontrol Yeast Rhodotorula glutinis Strain LS11 on Patulin Accumulation in Stored Apples     

Castoria R  Morena V  Caputo L  Panfili G  De Curtis F  De Cicco V 《Phytopathology》2005,95(11):1271-1278

ABSTRACT Contamination of apples (Malus domestica) and derived juices with fungicide residues and patulin produced by Penicillium expansum are major issues of food safety. Biocontrol agents represent an alternative or supplement to chemicals for disease control. Our data show that these microbes could also contribute to actively decreasing patulin accumulation in apples. Three biocontrol agents, Rhodotorula glutinis LS11, Cryptococcus laurentii LS28, and Aureobasidium pullulans LS30, were examined for their in vitro growth in the presence of patulin and for their capability to decrease mycotoxin recovery from the medium. Strain LS11 yielded the highest growth rates and the greatest decrease of toxin recoveries. Further, it caused the appearance of two major spots on thin-layer chromatography (TLC) plates, suggesting possible metabolization of the mycotoxin. In vivo, i.e., in the low percentage of LS11-pretreated apples infected by P. expansum, patulin accumulation was significantly lower than in nontreated infected fruits. Yeast cells survived and increased in infected apples and, in a model system emulating decaying apple, resulted in accelerated breakdown of patulin and the production of the same TLC spots as those detected in vitro. These data suggest that biocontrol yeast cells surviving in decaying apples could metabolize patulin and/or negatively affect its accumulation or synthesis. To our knowledge, this is the first report describing the effect of a biocontrol agent on patulin accumulation in vivo.  相似文献   

Development of mutants with varying flowering times by targeted editing of multiple SVP gene copies in Brassica napus L.     

Sunny Ahmar  Yungu Zhai  Huibin Huang  Kaidi Yu  Muhammad Hafeez Ullah Khan  Muhammad Shahid  Rana Abdul Samad  Shahid Ullah Khan  Olalekan Amoo  Chuchuan Fan  Yongming Zhou 《作物学报(英文版)》2022,(1):67-74

Manipulation of flowering time to develop cultivars with desired maturity dates is fundamental in plant breeding.It is desirable to generate polyploid rapeseed(Brassica napus L.)germplasm with varying flowering time controlled by a few genes.In the present study,Bna SVP,a rapeseed homolog of the Arabidopsis SVP(Short Vegetative Phase)gene,was characterized and a set of mutants was developed using a CRISPR/Cas9-based gene-editing tool.A single construct targeting multiple sites was successfully applied to precisely mutate four copies of Bna SVP.The induced mutations in these copies were stably transmitted to subsequent generations.Homozygous mutants with loss-of-function alleles and free transgenic elements were generated across the four Bna SVP homologs.All mutant T₁lines tested in two environments(summer and winter growing seasons)showed early-flowering phenotypes.The decrease in flowering time was correlated with the number of mutated Bna SVP alleles.The quadruple mutants showed the shortest flowering time,with a mean decrease of 40.6%–50.7%in length relative to the wild type under the two growth conditions.Our study demonstrates the quantitative involvement of Bna SVP copies in the regulation of flowering time and provides valuable resources for rapeseed breeding.  相似文献   

Purification of a Low Molecular Weight Fucoidan for SPECT Molecular Imaging of Myocardial Infarction     

Pierre Saboural  Frédéric Chaubet  Francois Rouzet  Faisal Al-Shoukr  Rana Ben Azzouna  Nadia Bouchemal  Luc Picton  Liliane Louedec  Murielle Maire  Lydia Rolland  Guy Potier  Dominique Le Guludec  Didier Letourneur  Cédric Chauvierre 《Marine drugs》2014,12(9):4851-4867

Fucoidans constitute a large family of sulfated polysaccharides with several biochemical properties. A commercial fucoidan from brown algae, containing low molecular weight polysaccharidic species constituted of l-fucose, uronic acids and sulfate groups, was simply treated here with calcium acetate solution. This treatment led to a purified fraction with a yield of 45%. The physicochemical characterizations of the purified fucoidan using colorimetric assay, MALLS, dRI, FT-IR, NMR, exhibited molecular weight distributions and chemical profiles similar for both fucoidans whereas the sulfate and l-fucose contents increased by 16% and 71%, respectively. The biodistribution study in rat of both compounds labeled with ^99mTc evidenced a predominant renal elimination of the purified fucoidan, but the crude fucoidan was mainly retained in liver and spleen. In rat myocardial ischemia-reperfusion, we then demonstrated the better efficiency of the purified fucoidan. This purified sulfated polysaccharide appears promising for the development of molecular imaging in acute coronary syndrome.  相似文献   

Degradation of Carotenoids in Apricot (Prunus armeniaca L.) During Drying Process     

Alessandra Fratianni  Donatella Albanese  Rossella Mignogna  Luciano Cinquanta  Gianfranco Panfili  Marisa Di Matteo 《Plant foods for human nutrition (Dordrecht, Netherlands)》2013,68(3):241-246

Carotenoids are natural compounds whose nutritional importance comes from the provitamin A activity of some of them and their protection against several serious human disorders. The degradation of carotenoids was investigated during apricot drying by microwave and convective hot-air at 60 and 70 °C. Seven carotenoids were identified: antheraxanthin, lutein, zeaxanthin, β-cryptoxanthin, 13-cis-β-carotene, all-trans-β-carotene and 9-cis-β-carotene; among these, all-trans-β-carotene was found to be about 50 % of total carotenoids. First-order kinetic models were found to better describe all-trans-β-carotene reduction during drying, with a degradation rate constant (k₁) that increased two folds when temperatures increased by 10 °C, in both methods. No differences were found in k₁ between apricots dried by hot air at 70 °C (k₁?=?0.0340 h^?1) and by microwave at 60 °C. The evolution of total carotenoids (117.1 mg/kg on dry basis) during drying highlighted a wider decrease (about 50 %) when microwave heating was employed, for both set temperatures. Antheraxantin was found to be the carotenoid most susceptible to heat, disappearing at 6 h during both trials with microwave as well as during convective hot-air at 70 °C. For this reason, antheraxanthin could be a useful marker for the evaluation of thermal damage due to the drying process. Also the degree of isomerization of all-trans-β-carotene could be a useful marker for the evaluation of the drying process.  相似文献