Identification of equine herpesvirus 3 (equine coital exanthema virus), equine gammaherpesviruses 2 and 5, equine adenoviruses 1 and 2, equine arteritis virus and equine rhinitis A virus by polymerase chain reaction     

K DYNON  A VARRASSO  N FICORILLI  SA HOLLOWAY  GH REUBEL  F LI  CA HARTLEY  MJ STUDDERT  HE DRUMMER 《Australian veterinary journal》2001,79(10):695-702

OBJECTIVE: To develop rapid (< 8 hour) tests using polymerase chain reaction (PCR) for the diagnosis of equine herpesvirus 3 (EHV3; equine coital exanthema virus), equine gammaherpesviruses 2 (EHV2) and EHV5, equine adenovirus 1 (EAdV1), EAdV2, equine arteritis virus (EAV), equine rhinitis A virus (ERAV; formerly equine rhinovirus 1) DESIGN: Either single round or second round (seminested) PCRs were developed and validated. METHODS: Oligonucleotide primers were designed that were specific for each virus, PCR conditions were defined and the specificity and sensitivity of the assays were determined. The application of the tests was validated using a number of independent virus isolates for most of the viruses studied. The PCRs were applied directly to clinical samples where samples were available. RESULTS: We developed a single round PCR for the diagnosis of EHV3, a seminested PCR for EHV2 and single round PCRs for EHV5, EAdV1, EAdV2 and RT-PCRs for EAV and ERAV. The PCR primer sets for each virus were designed and shown to be highly specific (did not amplify any recognised non-target template) and sensitive (detection of minimal amounts of virus) and, where multiple virus isolates were available all isolates were detected. CONCLUSION: The development and validation of a comprehensive panel of PCR diagnostic tests, predominantly for viruses causing equine respiratory disease, that can be completed within 8 hours from receipt of clinical samples, provides a major advance in the rapid diagnosis or exclusion diagnosis of these endemic equine virus diseases in Australia.  相似文献   

A longitudinal study of iron status in children at 12, 24 and 36 months     

Freeman VE  Mulder J  van't Hof MA  Hoey HM  Gibney MJ 《Public health nutrition》1998,1(2):93-100

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Predicting percentage of individuals consuming foods from percentage of households purchasing foods to improve the use of household budget surveys in estimating food chemical intakes     

Lambe J  Kearney J  Becker W  Hulshof K  Dunne A  Gibney MJ 《Public health nutrition》1998,1(4):239-247

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The influence of eating location on nutrient intakes in Irish adults: implications for developing food-based dietary guidelines     

O'Dwyer NA  Gibney MJ  Burke SJ  McCarthy SN 《Public health nutrition》2005,8(3):258-265

OBJECTIVES: To examine the contribution of the food service sector to the nutrient quality of the Irish diet, and to compare intakes at home, work and outside the home ('out') and within the subgroups of the out location (pub, deli, takeaway). DESIGN AND SETTING: Random sample of adults from the Republic of Ireland. Food intake data were collected using a 7-day food diary. Respondents recorded the location of every eating occasion determined by where the food was prepared rather than consumed. RESULTS: Intakes of energy, protein, fat and carbohydrate were significantly greater at home than at work or out (P<0.05). The intake of alcohol was significantly (P<0.001) greater out than at home or work. The percentage contribution of fat to energy was above the recommendations (33% of total energy and 35% of food energy) for both men and women at all locations, with the exception of the contribution of fat to total energy for men at the out location. Within the subgroups of the out location, the contribution of alcohol to total energy was greatest in pubs and the contribution of fat to both total and food energy was greatest in takeaways. Intakes of fibre and most micronutrients per 10 MJ of food energy were greater (P<0.05) at home than at work or out. CONCLUSION: Foods eaten outside the home contribute a disproportionately high level of fat intake and should be targeted in public health nutrition strategies.  相似文献   

Sociodemographic determinants of perceived influences on food choice in a nationally representative sample of Irish adults     

Kearney M  Kearney J  Dunne A  Gibney M 《Public health nutrition》2000,3(2):219-226

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Analysis of the temporal intake of cereal and dairy products in Irish adults: implications for developing food-based dietary guidelines     

Burke SJ  McCarthy SN  O'Dwyer NA  Gibney MJ 《Public health nutrition》2005,8(3):238-248

OBJECTIVES: To analyse the temporal distribution of the intake of cereal and dairy products in the Republic of Ireland. DESIGN: The North/South Ireland Food Consumption Survey established a database of habitual food and drink consumption using a 7-day food diary. The database also recorded the time and day of food consumption. Mean intakes of cereal and dairy products were calculated for time of the day and day of the week. RESULTS: At the weekend, the percentage of consumers decreased for nearly all cereal and dairy products. White bread, total cereals, full-fat milk and total dairy intakes were significantly lower at the weekend (P<0.01) compared with weekdays. Intakes of cereal and dairy products over time of the day showed clear mealtime or snacking patterns when the number of consumers was controlled for. White bread, wholemeal bread, total cereals, full-fat milk, reduced-fat milk and total dairy intakes showed mealtime peaks for morning, afternoon and evening. When examined by tertile of intake, tertile of percentage energy from fat and tertile of fibre intake, intakes of cereal and dairy products over time of the day and day of the week were similar to trends described above, regardless of the tertile. CONCLUSIONS: Temporal analysis of the intakes of cereal and dairy products did not reveal any unusual trends in this population. However, the significant methodological issues raised in this paper will be of benefit to other aspects of research in this area.  相似文献   

Neck wattles in lambs     

MJ LANCASTER  WD MEDWELL 《Australian veterinary journal》1991,68(2):75-76

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Experimental vaccinia virus infection of horses   总被引：1，自引：0，他引：1  

MJ STUDDERT 《Australian veterinary journal》1989,66(5):157-159

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Irish consumers' use and perception of nutrition and health claims     

Lynam AM  McKevitt A  Gibney MJ 《Public health nutrition》2011,14(12):2213-2219

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Oscillations in NF-kappaB signaling control the dynamics of gene expression     

Nelson DE  Ihekwaba AE  Elliott M  Johnson JR  Gibney CA  Foreman BE  Nelson G  See V  Horton CA  Spiller DG  Edwards SW  McDowell HP  Unitt JF  Sullivan E  Grimley R  Benson N  Broomhead D  Kell DB  White MR 《Science (New York, N.Y.)》2004,306(5696):704-708

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