Near-infrared spectroscopy in saffron quality control: determination of chemical composition and geographical origin     

Zalacain A  Ordoudi SA  Díaz-Plaza EM  Carmona M  Blázquez I  Tsimidou MZ  Alonso GL 《Journal of agricultural and food chemistry》2005,53(24):9337-9341

Near-infrared reflectance spectroscopy has been applied for the first time to saffron spice to determine the chemical composition and geographical origin of 111 samples from the there main producers' countries: Iran, Greece, and Spain. The validation procedures with the results obtained by UV-vis and HPLC-DAD measurements demonstrated that this technique is appropriate to determine the following parameters: moisture and volatile content, coloring strength, (250 nm), and (330 nm), established on the ISO 3632 Technical Specification Normative and used to certify saffron quality in the international market. Also, it can be used to estimate the content of the five main crocetin glycosides, the compounds responsible for saffron color, the best correlations being for trans-crocetin di-(beta-D-gentibiosyl) ester (R2= 0.93), trans-crocetin (beta-D-glucosyl)-(beta-D-gentibiosyl) (R2= 0.94), and picrocrocin (R2= 0.92), the compound accepted as responsible for saffron bitterness. Finally, a discriminant analysis among the three geographical origins reveals that Iranian samples are the most different, whereas Greek and Spanish samples are more similar. All of these results reveal that NIRS spectroscopy has an enormous potential for its application to saffron quality control as the results are obtained in 2 min and without any sample manipulation.  相似文献   

MALDI-TOF, HPLC-ESI-TOF and 13C-NMR characterization of chestnut (Castanea sativa) shell tannins for wood adhesives     

Gonzalo Vázquez  Antonio Pizzi  M. Sonia Freire  Jorge Santos  Gervasio Antorrena  Julia González-Álvarez 《Wood Science and Technology》2013,47(3):523-535

MALDI-TOF, HPLC-ESI-TOF and 13C-NMR techniques were used to analyse the structure of non-purified aqueous chestnut shell tannin extracts. In addition, the influence of the extraction agent (water or aqueous solutions of Na₂SO₃ and/or NaOH) on tannin structure was analysed by MALDI-TOF in order to select the extract with the best properties for wood adhesives. Using HPLC-ESI-TOF, catechin/epicatechin, gallocatechin/epigallocatechin, dicatechin structures, dicatechin structures without a hydroxyl group, galloyl-glucoses and ellagic acid were identified as the main monomeric components in the aqueous extract. 13C-NMR and MALDI-TOF spectra revealed that extracts are mostly composed of procyanidin and prodelphinidin structures although prorobinetidins might be also present. MALDI-TOF spectrometry was used to identify the extract oligomeric components. Extractions with Na₂SO₃ and/or NaOH produced changes in the predominant structures in the extracts and on the amount of sugar units linked to the flavonoid structures, which decreased in the presence of Na₂SO₃. Extract obtained using low Na₂SO₃ and NaOH concentrations (1.5 and 0.75 %, respectively) was selected as more suitable for wood adhesive preparation.  相似文献   

Pyroptosis and adaptive immunity mechanisms are promptly engendered in mesenteric lymph-nodes during pig infections with Salmonella enterica serovar Typhimurium     

Rodrigo Prado Martins  Carmen Aguilar  James E Graham  Ana Carvajal  Rocío Bautista  M Gonzalo Claros  Juan J Garrido 《Veterinary research》2013,44(1):120

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A detailed molecular analysis of complete Bovine Leukemia Virus genomes isolated from B-cell lymphosarcomas     

Gonzalo Moratorio  Sabrina Fischer  Sergio Bianchi  Lorena Tomé  Gonzalo Rama  Gonzalo Obal  Federico Carrión  Otto Pritsch  Juan Cristina 《Veterinary research》2013,44(1):19

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ORIGINS MATTER: (NO) MARKET INTEGRATION BETWEEN CULTURED AND WILD GILTHEAD SEA BREAM IN THE SPANISH SEAFOOD MARKET     

Gonzalo Rodríguez Rodríguez  Roberto Bande  Sebastián Villasante 《Aquaculture Economics & Management (Blackwell Science)》2013,17(4):380-397

Interactions between fisheries and aquaculture have become a major issue that is still insufficiently studied. Therefore, the objective of this article is to test whether the cultured and wild gilthead sea bream in the Spanish seafood markets are integrated. By using the Johansen methodology, cointegration of the price series of farmed and wild gilthead sea bream was tested. In contrast to previous studies, our econometric results show that wild and the farmed gilthead sea bream form two heterogeneous products in the Spanish market. These new results question the generalization currently accepted by the scientific literature that farmed and wild fish are substitutes when they belong to the same species. The binomial product-market, along with some specific features of the Spanish market, such as the negative perceptions of aquaculture by some significant groups of consumers, appeared to have a great explanatory power for justifying these different results.  相似文献   

Leaf classification in sunflower crops by computer vision and neural networks   总被引：2，自引：0，他引：2  

Juan Ignacio Arribas  Gonzalo V. Sánchez-FerreroGonzalo Ruiz-Ruiz  Jaime Gómez-Gil 《Computers and Electronics in Agriculture》2011,78(1):9-18

In this article, we present an automatic leaves image classification system for sunflower crops using neural networks, which could be used in selective herbicide applications. The system is comprised of four main stages. First, a segmentation based on rgb color space is performed. Second, many different features are detected and then extracted from the segmented image. Third, the most discriminable set of features are selected. Finally, the Generalized Softmax Perceptron (GSP) neural network architecture is used in conjunction with the recently proposed Posterior Probability Model Selection (PPMS) algorithm for complexity selection in order to select the leaves in an image and then classify them either as sunflower or non-sunflower. The experimental results show that the proposed system achieves a high level of accuracy with only five selected discriminative features obtaining an average Correct Classification Rate of 85% and an area under the receiver operation curve over 90%, for the test set.  相似文献   

Land use effects on microbial biomass C, β-glucosidase and β-glucosaminidase activities,and availability,storage, and age of organic C in soil   总被引：1，自引：0，他引：1  

David Sotomayor-Ramírez  Yusmary Espinoza  Veronica Acosta-Martínez 《Biology and Fertility of Soils》2009,45(5):487-497

Microbial biomass, β-glucosidase and β-glucosaminidase activities, and availability, storage, and age of soil organic C were investigated after 26 years of conversion from sugarcane (Saccharum officinarum) to forest (Eucaliptus robusta or Leucaena leucocephala), pasture (mixture of tropical grasses), and to vegetable cropping (agriculture) in a vertisol in Puerto Rico. Soil organic C (SOC) at 0–100 cm was similar under Leucaena (22.8 kg C/m²), Eucalyptus (18.6 kg C/m²), and pasture (17.2 kg C/m²), which were higher than under agriculture (13.0 kg C/m²). Soil organic N (SON) at 0–100 cm was similar under the land uses evaluated which ranged from 1.70 (under agriculture) to 2.28 kg N/m² (under Leucaena forest). Microbial biomass C (MBC) and N (MBN) of the 0–15-cm soil layer could be ranked as: pasture > Leucaena = Eucalyptus > agriculture. The percentages of SOC and SON present as MBC and MBN, respectively, were nearly 1% in pasture and less than 0.50% in forest under Leucaena or Eucalyptus and agricultural soil. The activity of β-glucosidase of the 0–15-cm soil layer could be ranked as: Leucaena = Eucalyptus > pasture > agriculture; while β-glucosaminidase activity was ranked as: Eucalyptus > Leucaena = pasture > agriculture. The soil δ ¹³C changed from 1996 to 2006 in forest under Eucalyptus (18.7‰ to 21.2‰), but not under Leucaena (20.7‰ to 20.8‰). The soil under Leucaena preserved a greater proportion of old C compared to the forest under Eucalyptus; the former had an increased soil mineralizable C from the current vegetation inputs. The soil under agriculture had the lowest enzyme activities associated with C cycling, lowest percentage of SOC as MBC, highest percentage of SOC present as mineralizable C, and highest percentage of MBC present as mineralizable C compared to the other land uses.  相似文献   

Effect of temperature increase on the embryonic development of Patagonian red octopus Enteroctopus megalocyathus in controlled culture          下载免费PDF全文

Iker Uriarte  Emmanuel Martínez‐Montaño  Viviana Espinoza  Carlos Rosas  Jorge Hernández  Ana Farías 《Aquaculture Research》2016,47(8):2582-2593

One of the major problems involved in the controlled cultivation of Patagonian red octopus (Enteroctopus megalocyathus) is its long embryonic period ranging between 150–176 days, after which the hatching of planktonic paralarvae is achieved. The effect of temperature on the incubation of E. megalocyathus eggs was studied with the aim of establishing if a temperature higher than 12°C is effective to accelerate the embryonic development without altering their morphological and physiological conditions. Fertilized eggs obtained under controlled conditions at 11°C ± 0.1 were randomly distributed in 12 water baths of 30 L at 4 temperatures: 12, 14, 15 and 16°C ± 0.1°C. The experiment lasted until egg hatching occurred.The embryo growth rate was accelerated at 15–16°C, so the time spent in embryonic development can be reduced in 15% when compared with embryo development obtained from eggs incubated at 12°C. The embryos showed no significant differences in the final survival and were morphometrically similar in all stages of development at all temperatures. The increase in temperature from 12 to 16°C, even if it allowed a better growth, had high metabolic costs for embryos of E. megalocyathus. The activities of lipases and proteases were affected by interaction between temperature and the embryo stage, with high lipase activity observed in embryos of stage XV incubated at high temperatures and the highest levels of trypsin and chymotrypsin in stage XX at 14°C. The results suggest that 15°C could be the limit temperature to increase growth.  相似文献   

Changes in drought tolerance of <Emphasis Type="Italic">Pinus radiata</Emphasis> in Chile associated with provenance and breeding generation     

Sergio E. Espinoza  Carlos R. Magni  Rómulo E. Santelices  Milǒs Ivković  Antonio M. Cabrera 《Annals of Forest Science》2016,73(2):267-275

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Extraction of antioxidants from eucalyptus (Eucalyptus globulus) bark     

Gonzalo Vázquez  Jorge Santos  M. Sonia Freire  Gervasio Antorrena  Julia González-álvarez 《Wood Science and Technology》2012,46(1-3):443-457

The extraction of phenolic compounds from eucalyptus (Eucalyptus globulus) bark was examined with the aim of analyzing the potential of the extracts as natural antioxidants. Experiments were planned according to a 2³ factorial design to analyze the influence of temperature and Na₂SO₃ and NaOH concentrations in aqueous solutions on extraction yield, extract total phenols content, ferric reducing antioxidant power (FRAP), and number- and weight-average molecular weights. Extract total phenols content and FRAP antioxidant activity in the ranges 0.91–2.58?g gallic acid equivalent (GAE)/100?g oven-dried bark and 4.70–11.96?mmol ascorbic acid equivalent (AAE)/100?g oven-dried bark, respectively, demonstrated the potential of eucalyptus bark as a source of antioxidant compounds. Extraction at the highest temperature (100°C), the lowest Na₂SO₃ concentration (1.5% on oven-dried bark), and without NaOH provided the highest extract total phenols content and FRAP antioxidant activity. Those eucalyptus bark extracts with lower molecular weight showed higher antioxidant activity. Matrix-assisted laser desorption ionization time-of-flight and reverse-phase high-performance liquid chromatography electrospray ionization time-of-flight mass spectrometry revealed the presence of polygalloyl glucoses, catechin, epicatechin, ellagic acid, quercetin-3-o-rhamnoside, and isorhamnetin in eucalyptus bark aqueous extracts.  相似文献