Genetic improvement of legumes using somatic cell and molecular techniques     

V. Kumar  M. R. Davey 《Euphytica》1991,55(2):157-169

Summary The merits and limitations of somatic cell techniques involving Agrobacterium-mediated transformation, direct gene transfer and protoplast fusion, are discussed in relation to the genetic improvement of forage and grain legumes. Whilst progress with legumes is limited compared to that with plants of other families such as the Solanaceae, the fact that many legumes are readily amenable to tissue culture now permits somatic cell techniques to be targetted to these species. Future development of the subject will necessitate close collaboration between molecular biologists and plant breeders to enable novel plants generated by in vitro technologies to be incorporated into conventional breeding programmes.  相似文献   

The Influence of Plant Developmental Stage on Expression of Resistance to leaf Rust (Puccinia recondita) in Two Near Isogenic Lines of Wheat     

Rajindra  Kumar  R. N. Sawhney    J. B. Sharma  V. L. Chopra 《Plant Breeding》1988,100(3):225-227

Cultivar ‘Thatcher’, and ‘Thatcher’ lines with Lr 21 and Lr 22 were studied against a number of races of Puccinia recondita for seedling and adult plant reaction. The study has established that Lr 21 and Lr 22 are genes effective against P. recondita at adult plant stage. It has also shown that these genes confer resistance against all races when plants are inoculated at boot leaf stage.  相似文献   

Resistance to wilt in chickpea. II. Further evidence for two genes for resistance to race 1     

H. D. Upadhyaya  J. B. Smithson  M. P. Haware  J. Kumar 《Euphytica》1983,32(3):749-755

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Fertility Improvement in Autotetraploids of Pea — Selection for Seed-Set and Disjunction Index     

H. Kumar    V. C. Mercykutty  C. P. Srivastava 《Plant Breeding》1993,110(1):81-83

Four seed-set groups of plants, viz. unselected ‘general’ and selected ‘high’, ‘medium’ and ‘low’ were constituted in C₁ generation from randomly selected autotetraploids of two pea cultivars, T 163 and 5064-S. In C₂ generation, ten plants were selected at random in each of the four groups. Two seeds each from selected autotetraploids were drawn and bulked to make 20 seeds in each of the four groups to raise C₃ generation. Selection resulted in a gradual shift in seed-set, though the magnitude and nature varied with the group and generation. Improvement in the seed-set from C₁ to C₃ generation was associated with the increase in bivalents per cell, disjunction index and total chiasmata. Increase in total chiasmata was mostly due to an increase in proximal ones. Increase in seed-set was reflected in improvement of grain yield per plant.  相似文献   

A unique introgression from Moricandia arvensis confers male fertility upon two different cytoplasmic male-sterile lines of Brassica juncea     

S. R. Bhat    S. Prakash    P. B. Kirti    V. Dinesh  Kumar V. L. Chopra 《Plant Breeding》2005,124(2):117-120

A Brassica juncea line carrying an introgression from Moricandia arvensis restored male fertility to two cytoplasmic male‐sterile (CMS) B. juncea lines carrying either M. arvensis or Diplotaxis catholica cytoplasm. Genetics of fertility restoration was studied in the F₁, F₂, F₃ and backcross generations of the cross between CMS and fertility‐restorer lines. No male‐sterile plants were found in F1‐F₃ generations of the cross between CMS [M. arvensis] B. juncea and the restorer. However, a 1: 1 segregation for male sterility and fertility was observed when the F1 was pollinated with non‐restorer pollen from a euplasmic line. These results clearly show that restoration is mono‐genic and gametophytic. In CMS lines carrying D. catholica cytoplasm, the restorer conferred male fertility to the F1 and showed 3: 1 and 1: 1 segregations for male fertility and sterility in F₂ and BC1 generations, respectively, indicating a monogenic, sporophytic mode of fertility restoration. The results were also supported by pollen stainability in the F1 which was about 65% in M. arvensis‐based CMS and >90% in D. catholica‐based CMS. The above results are discussed in the light of previous molecular studies which showed association between CMS and atpA in both systems.  相似文献   

Initial screening of chili and sweet pepper germplasm for resistance to chili thrips, Scirtothrips dorsalis Hood     

N. K. Krishna Kumar  M. Aradya  A. A. Deshpande  N. Anand  P. R. Ramachandar 《Euphytica》1996,89(3):319-324

Summary A preliminary evaluation for resistance to chili thrips, Scirtothrips dorsalis Hood of 41 and 194 pepper (Capsicum annuum L.) germplasms during 1987 and 1988, respectively, indicated chili accessions may be a promising source of resistance. In contrast, all sweet pepper accessions tested were highly susceptible. A number of chili accessions produced a moderate yield, in spite of a high thrips infestation indicating tolerance to S. dorsalis. Rating for thrips damage was more reliable and efficient than estimating thrips numbers in screening pepper accessions for resistance to thrips. Highly significant, positive correlation between ratings at the seedling stage and final rating for thrips damage indicated the feasibility of screening pepper accessions at the seedling stage. Comparing the similarities in rating among accessions resulted in 40 distinct groups. The variance-covariance matrix of the data from these 40 groups was subjected to principal component analysis. This accounted for 56 and 18 per cent of the variation across the two principal axes, respectively. Projection of chili and sweet pepper accessions along these two axes revealed three distinct clusters. About 80 per cent of chili accessions formed the first cluster, 58 per cent of the sweet pepper accessions formed the second cluster and a third cluster exhibited intermediate ratings for thrips damage. The significance of these findings in relation to geographical divergence and resistance to thrips among pepper accessions is discussed.  相似文献   

QTL analysis for phytonutrient compounds and the antioxidant molecule in mustard (Brassica juncea L.)     

Shilpa Gupta  M. K. Sangha  Gurpreet Kaur  Shashi Banga  Mehak Gupta  Hitesh Kumar  S. S. Banga 《Euphytica》2015,201(3):345-356

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Genomic regions associated with grain yield under drought stress in wheat (Triticum aestivum L.)     

Sanyukta Shukla  Kalpana Singh  Rajendra V. Patil  Suhas Kadam  Sudhakar Bharti  Pratti Prasad  Nagendra Kumar Singh  Renu Khanna-Chopra 《Euphytica》2015,203(2):449-467

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Influence of cyto‐sterility sources of female line on seed quality of Indian mustard (Brassica juncea L. Czern & Coss.) in relation to storage period          下载免费PDF全文

Shyamal Kumar Chakrabarty  Aniruddha Maity  Jung Bahadur Yadav 《Plant Breeding》2015,134(3):333-337

Effect of cyto‐sterility sources on morphology and yield attributes in F₁ has well been studied in different crops, but not on the seed quality of hybrid. Six cytoplasmic male sterile (CMS) lines, viz. Ogura, Siifolia, Erucoides, Moricandia, Tournefortii and Oxyrrhina, which represent six different sources of CMS, were pollinated by a single maintainer line (‘Pusa Bold’) to check whether CMS sources have any marked effect on seed quality. Seeds were collected in 2007–2008 (rabi season: October–April) from CMS maintenance plot and kept in ambient storage for next 3 years. The results indicated that CMS/cyto‐sterility sources influenced the seed quality parameters in fresh seed as well as after storage. Per cent germination (as means over the storage period) was recorded up to 89.00, 88.25 and 87.88 in Moricandia, Ogura and Oxyrrhina systems, respectively, and it was significantly different from Tournefortii and ‘Pusa Bold’ (70.13 and 74.38, respectively). The CMS sources also had pronounced effect on other seed quality traits, viz. root and shoot length and seedling dry matter, and Ogura and Moricandia (green) performed better.  相似文献   

Aluminium tolerance in lentil (Lens culinaris Medik.) with monogenic inheritance pattern          下载免费PDF全文

Dharmendra Singh  Harsh Kumar Dikshit  Arun Kumar 《Plant Breeding》2015,134(1):105-110

The objectives of this study were to determine genetics of Al tolerance and whether the Al tolerance observed is governed by the same gene. The lines ‘L‐7903’ and ‘L‐4602’ have been developed through breeding programme as Al‐tolerant lines. These lines showed maximum root regrowth and minimum accumulation of Al and callose as compared to sensitive genotypes (‘BM‐4’ and ‘L‐4147’). Al tolerance in the parents, F₁, F₂ and backcross generations was estimated using the regrowth of the primary root after staining and scoring of fluorescent signals. The F₁ hybrids responded similarly to the tolerant parents, indicating dominance of Al tolerance over sensitivity. The segregation ratios obtained for Al tolerance and sensitivity in the F₂ and backcross generations were 3 : 1 and 1 : 1, respectively. Test of allelism confirmed the same gene was conferring Al tolerance in both genotypes (‘L‐7903’ and ‘L‐4602’) as the F₁ was also tolerant and no segregation of tolerant : sensitive was recorded. These results indicated that Al tolerance is a monogenic dominant trait that can be easily transferred to agronomic bases through backcross breeding technique.  相似文献