Characterization of a gram-positive bacterium from the proventriculus of budgerigars (Melopsittacus undulatus)     

C M Scanlan  D L Graham 《Avian diseases》1990,34(3):779-786

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Novel Strategies in the design and production of vaccines Sara Cohen and Avigdor Shafferman (editors), 1996, vol. 397, Advances in Experimental Medicine and Biology, Plenum, ISBN 0-306-45211-1, 214 pp., $75.00/$90.00 outside USA and Canada     

H. Graham Purchase 《Preventive veterinary medicine》1997,31(3-4):293-295

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Effect of site preparation on survival and growth of Douglas-fir (Pseudotsuga menziessi Mirb. Franco.) seedlings     

Russell T. Graham  Alan E. Harvey  Martin F. Jurgensen 《New Forests》1989,3(1):89-98

The performance (survival and growth) of Douglas-fir (Pseudotsuga menziesii Mirb. Franco.) seedlings planted in minimally disturbed, scalped, and bedded soils, both with and without herbicidal control of weeds, were compared. Douglas-fir growing for 3 years in bedded soils treated with herbicide were heavier, taller, and had deeper root systems than trees growing in other preparations. Scalping did not improve seedling performance when compared to minimally disturbed soils. Soils rich in organic matter benefited tree growth. Competing vegetation in raised beds was detrimental to seedling performance.  相似文献   

Environmental and physiological controls over oxygen and carbon isotope composition of Tasmanian blue gum, Eucalyptus globulus     

Cernusak LA  Farquhar GD  Pate JS 《Tree physiology》2005,25(2):129-146

We measured oxygen isotope ratios (delta18O) of xylem sap, phloem sap, leaves, wood and bark of Eucalyptus globulus Labill. growing in southwestern Australia. Carbon isotope ratios (delta13C) were measured in the dry matter of phloem sap, leaves and wood. Results were used to test several aspects of a mechanistic model of 18O enrichment and provided insights into post-photosynthetic variations in dry matter delta13C. Xylem water delta18O varied little within the tree crown, whereas variation at the landscape-level was more pronounced, with plantations near the coast being enriched by up to 3 per thousand compared with plantations less than 100 km inland. Phloem water was significantly enriched in 18O compared with xylem water in two of three sampling campaigns; mean enrichments were 0.5 and 0.8 per thousand. Phloem sap sugars exported from E. globulus leaves closely reflected observed leaf water enrichment when diurnal variation in photosynthesis was taken into account. Photosynthetic rates were higher in the morning than in the afternoon, whereas leaf water 18O enrichment increased to maximum values in the afternoon. A non-steady-state model of leaf water 18O enrichment accurately predicted observed values through a full diel cycle. Mean estimates of the proportion of organic oxygen effectively exchanging with xylem water during cellulose synthesis were close to 0.40 for both leaves and wood. Carbon isotope ratios of nascent xylem tissues did not differ from those of phloem sap sugars collected concurrently, whereas nascent leaf tissues were depleted in 13C by 2 per thousand compared with phloem sap sugars, suggesting that, in E. globulus, 13C enrichment of sink tissues compared with source leaves does not result from an enriching process within the sink tissue.  相似文献   

Use of pyrethroid analogues to identify key structural features for enhanced esterase resistance in Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae)     

Gunning RV  Moores GD  Jewess P  Boyes AL  Devonshire AL  Khambay BP 《Pest management science》2007,63(6):569-575

It has been reported previously that the major resistance mechanism to pyrethroid insecticides by the cotton bollworm Helicoverpa armigera (Hübner) in Australia is a consequence of overproduction of esterase isoenzymes. This paper reports structure-activity relationships that support such a view, based on in vivo bioassays conducted with a range of pyrethroid structures containing a variety of acid and alcohol moieties and the correlation with in vitro esterase inhibition assays against the same structures, and identifies the critical regions of the molecule with regard to esterase inhibition, and hence resistance. The implications of this work in terms of possible resistance management are evaluated and discussed.  相似文献   

Catastrophic Acute Ischemic Stroke After Crotalidae Polyvalent Immune Fab (Ovine)-Treated Rattlesnake Envenomation     

Sean P. Bush  Graham G. Mooy  Tammy H. Phan 《The Forestry Chronicle》2014,25(2):198-203

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First isolation of sleeping disease virus from rainbow trout, Oncorhynchus mykiss (Walbaum), in the United Kingdom     

Graham DA  Rowley HM  Walker IW  Weston JH  Branson EJ  Todd D 《Journal of fish diseases》2003,26(11-12):691-694

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The antimicrobial activity of honey against common equine wound bacterial isolates     

R. Carnwath  E.M. Graham  K. Reynolds  P.J. Pollock 《Veterinary journal (London, England : 1997)》2014,199(1):110-114

Delayed healing associated with distal limb wounds is a particular problem in equine clinical practice. Recent studies in human beings and other species have demonstrated the beneficial wound healing properties of honey, and medical grade honey dressings are available commercially in equine practice. Equine clinicians are reported to source other non-medical grade honeys for the same purpose. This study aimed to assess the antimicrobial activity of a number of honey types against common equine wound bacterial pathogens. Twenty-nine honey products were sourced, including gamma-irradiated and non-irradiated commercial medical grade honeys, supermarket honeys, and honeys from local beekeepers. To exclude contaminated honeys from the project, all honeys were cultured aerobically for evidence of bacterial contamination. Aerobic bacteria or fungi were recovered from 18 products. The antimicrobial activity of the remaining 11 products was assessed against 10 wound bacteria, recovered from the wounds of horses, including methicillin resistant Staphylococcus aureus and Pseudomonas aeruginosa. Eight products were effective against all 10 bacterial isolates at concentrations varying from <2% to 16% (v/v). Overall, the Scottish Heather Honey was the best performing product, and inhibited the growth of all 10 bacterial isolates at concentrations ranging from <2% to 6% (v/v).Although Manuka has been the most studied honey to date, other sources may have valuable antimicrobial properties. Since some honeys were found to be contaminated with aerobic bacteria or fungi, non-sterile honeys may not be suitable for wound treatment. Further assessment of gamma-irradiated honeys from the best performing honeys would be useful.  相似文献   

A rapid immunoperoxidase-based virus neutralization assay for salmonid alphavirus used for a serological survey in Northern Ireland   总被引：1，自引：0，他引：1  

Graham DA  Jewhurst VA  Rowley HM  McLoughlin MF  Todd D 《Journal of fish diseases》2003,26(7):407-413

A modified virus neutralization (VN) assay was developed to replace an existing assay read on the presence or absence of virus-induced cytopathic effect (CPE). The modified assay used a monoclonal antibody to salmon pancreas disease virus as the first layer of an immunoperoxidase (IPX)-based immunostaining technique to detect viral growth. The IPX-based VN assay required only 3 days to perform, and the adoption of a 96-well microtitre format facilitated a high throughput of samples requiring small volumes of serum, cells and virus. When 352 sera from farmed salmon and 302 sera from farmed trout were tested by both the modified and the original CPE-based assays, overall correlations of 97.72 and 96.03% were, respectively, obtained (96.94% combined). When the modified assay was used to test 188 sera collected from wild salmonids in freshwater river systems in Northern Ireland, no positive results were recorded.  相似文献   

Effects of Added Shelter and Stocking Density on Growth of Sleepy Cod Oxyeleotris lineolatus in Ponds   总被引：2，自引：0，他引：2  

Brett W.  Herbert Peter A.  Graham Scot D.  Foster 《Journal of the World Aquaculture Society》2003,34(4):433-440

Sleepy cod Oxyeleotris lineolatus is a species of freshwater goby in demand in Australian markets by consumers of Asian origin. It is related to marble goby Oxyeleotris marmoratus , the most expensive freshwater food fish in Asia, which is cultured throughout southeast Asia in ponds and cages. The performance of sleepy cod in culture conditions was investigated to assess the viability of farming them in northern Australia. Sleepy cod fingerlings (62.8 ± 0.8 mm total length and 2.56 ± 0.095 g) were stocked into experimental ponds at 32,857 fish/ha, and grown out for 8 mo. Shelter was provided in each of three replicate ponds and was absent in three control ponds. The provision of shelter in juvenile growout was found to be of no benefit, although fish in ponds provided with shelter weighed slightly more per unit length than fish in ponds without shelter. Cannibalism was not a problem in growout, and survival was close to 100%. After the shelter trial was completed, fish were graded into large and small classes (three replicates of each), and grown out without shelter at the same density for 158 d. Following that, fish were again graded, and the largest 30% retained from growout at a density of 8,857 fish/ha (large, 198 ± 6.44 g) or 10,000 fish/ha (small, 48.9 ± 1.27 g). These were grown out for 188 d. Growth of selected stock at low densities was slower than earlier growth rates, although smaller fish gained weight more rapidly than larger fish. Growth rates were better than the only published data for marble goby. Further investigation into high density culture and different genotypes of sleepy cod needs to be undertaken to determine the viability of pond culture.  相似文献