Comparative evaluation of enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of dourine     

D A Wassall  R J Gregory  L P Phipps 《Veterinary parasitology》1991,39(3-4):233-239

The detection of antibodies against Trypanosoma equiperdum in 689 equid sera was compared by enzyme-linked immunosorbent assay (ELISA), the complement fixation test (CFT) and an indirect immunofluorescent test (IIF). CFT was the least sensitive technique, susceptible to anti-complementary factors and the most technically demanding. IIF was more sensitive, but was only suitable for testing limited numbers of samples. In this study, ELISA was the most sensitive test, the least labour intensive and lends itself to a considerable degree of automation. It is suggested that ELISA would be relatively easy to standardise between laboratories and an ELISA protocol could be adopted as the internationally approved test for equine health certification.  相似文献   

Prediction of breeding values for twinning rate and ovulation rate with a multiple trait, repeated records animal model.     

L D Van Vleck  K E Gregory  S E Echternkamp 《Journal of animal science》1991,69(10):3959-3966

A genetic correlation near unity between ovulation rate in heifers and later twinning frequency led to consideration of using measures of ovulation rate in heifers for each estrous cycle, beginning at puberty, to increase accuracy of selection for twinning rate. An initial evaluation with a multiple trait animal model for predicting breeding values included six genetic groups: 1) selected Scandinavian bulls, 2) transfers from other populations at the Research Center to a twinning project, 3) early-purchased Holsteins, 4) late-purchased Holsteins, 5) early purchases-other breeds, and 6) late purchases-other breeds. For ovulation and twin measures, heritabilities of .100 and .070 and repeatabilities of .120 and .092 were assumed. Assumed phenotypic correlation between ovulation and twin measures of .08 was accounted for by genetic correlation of .89 and permanent environmental correlation of .19. The number of animals evaluated was 1,745; 6,912 estrous cycles were measured for ovulation rate on 840 heifers and 1,929 parturitions were observed for occurrence of twinning on 851 cows, of which 346 had ovulation rate measured as heifers. The remaining 400 animals were foundation animals that created relationships among those with records or were sires of animals with records. The Scandinavian genetic group effect was substantially greater than that of the others. Joint evaluations were compared to evaluations using only twinning measures. For animals with twin evaluations based only on parents but with ovulation rates measured, the multiple trait evaluation increased accuracy of evaluation from .62 (twin information only) to .81.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

Toxoplasmosis in two cats with inflammatory intestinal disease   总被引：1，自引：0，他引：1  

J L Peterson  M D Willard  G E Lees  M R Lappin  T Dieringer  E Floyd 《Journal of the American Veterinary Medical Association》1991,199(4):473-476

Lymphocytic-plasmacytic enteritis, a chronic inflammatory intestinal disease, was diagnosed in 2 cats. In 1 cat, recurrence of clinical signs after initiating treatment was attributed to relapse of the inflammatory intestinal disease, but was found to be attributable to relapsing toxoplasmosis secondary to immunosuppressive drug therapy. Treatment with clindamycin resolved the recurrent toxoplasmosis. In the second cat, clinical signs of toxoplasmosis did not develop, but serologic testing yielded evidence of active toxoplasmosis. Treatment with clindamycin caused the titers to decrease. Relapsing toxoplasmosis may be responsible for apparent resistance to treatment in cats for inflammatory intestinal disease being treated with immunosuppressive drugs.  相似文献   

THE GALTON SOCIETY FOR THE STUDY OF THE ORIGIN AND EVOLUTION OF MAN     

Gregory WK 《Science (New York, N.Y.)》1919,49(1263):267-268

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Comparison of Insulin-like Growth Factor-I and Insulin-like Growth Factor Binding Protein Concentrations of the Palmetto and Sunshine Bass and the Effects of Gender and Stress     

Kenneth B.  Davis Brian C.  Peterson 《Journal of the World Aquaculture Society》2005,36(3):384-392

Abstract— Plasma concentrations of insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding proteins (IGFBPs) were compared between market sized sunshine and palmetto bass. Differences between male and female fish were examined and both hybrids were sampled before and after a low-water confinement stress. There was no difference in body weight between the hybrid types, however, females were significantly heavier than males in both hybrids. Plasma IGF-I concentrations were significantly higher in pre-stressed palmetto bass when compared with sunshine bass, and male palmetto bass had significantly higher IGF-I concentrations than females. Two hours after a 15-min low-water stress, a significant decrease in palmetto bass IGF-I occurred, but there was no change in sunshine bass IGF-I concentrations. Levels of a 33-kDa IGFBP were similar in pre-stressed palmetto bass when compared with sunshine bass; however, no differences due to gender were evident for either hybrid. Two hours after a 15-min low-water exposure, there was no change in the levels of a 33-kDa IGFBP in palmetto or sunshine bass. A more complete understanding of IGF-I, IGFBPs, and IGF-I receptors may be necessary to explain the role of IGF-I in regulating fish growth.  相似文献   

Contributions of ovum cytoplasm, uterine environment and postnatal environment to maternal effects in beef cattle     

R R Maurer  K E Gregory 《Journal of animal science》1990,68(8):2319-2332

In the first part of the experiment, embryos of Brown Swiss-Hereford (BS-H) reciprocal crosses were transferred to BS and H recipient cows and embryos of Red Poll-Angus (RP-A) reciprocal crosses were transferred to RP and A recipient cows. Calves resulting from embryo transfers (ET) were weaned early (3 to 5 d). Males were castrated and their growth, feed efficiency and carcass composition were recorded. In the second part of the experiment, matings to produce reciprocal crosses were made by natural service (NS) between BS and H and between RP and A. Some of the offspring were weaned at 3 to 5 d; the remainder nursed their dams to an age of 150 to 180 d. Growth after 200 d, feed efficiency and carcass data were recorded on steers. Females produced in both parts of the experiment were reared for breeding, and post-200-d growth and age at puberty were recorded. Neither breed of recipient (uterine influence) nor breed of donor (cytoplasmic influence) had important effects on growth or carcass traits. Large differences were observed for most growth and carcass traits between offspring that were weaned early and those that nursed their dams. Effects of early weaning were greater in progeny of Brown Swiss and Red Poll dams than in progeny of Angus and Hereford dams. Prenatal maternal influences (e.g., ovum cytoplasm and uterine influence) are relatively small in comparison with postnatal maternal influences in contributing to maternal effects in beef cattle.  相似文献   

Clinicopathologic features of suspected brevetoxicosis in double-crested cormorants (Phalacrocorax auritus) along the Florida Gulf Coast.     

Christine Kreuder  Jonna A K Mazet  Gregory D Bossart  Tim E Carpenter  Marcel Holyoak  Marc S Elie  Scott D Wright 《Journal of zoo and wildlife medicine》2002,33(1):8-15

Outbreaks of morbidity and mortality in double-crested cormorants (Phalacrocorax auritus) along Florida's Gulf Coast have occurred sporadically for at least 30 yr. During these outbreaks, the Clinic for the Rehabilitation of Wildlife, located on Sanibel Island in Florida, has admitted a substantial number of cormorants with consistent presentation of primarily neurologic clinical signs. In order to investigate the association of these outbreaks in cormorants with exposure to brevetoxin, we compared the timing of admittance of cormorants with outbreak-specific clinical signs to blooms of the brevetoxin-producing marine algae, Karenia brevis (formerly Gymnodinium breve), around Sanibel Island from 1995 through 1999. The clinic admitted 360 out of 613 cormorants with the common clinical sign of severe cerebellar ataxia in six outbreaks occurring during this period. The ataxia was characterized by a broad-based stance, truncal incoordination, hypermetric gait, and intention tremors of the head. The histopathologic findings in 10 cormorants euthanized in 1997 were mild and nonspecific. An immunohistochemical staining technique for the detection of brevetoxin in cormorants documented the uptake of brevetoxin in tissues from four cormorants admitted during an outbreak in 1997, but a modified technique used on samples from 11 cormorants admitted during a K. brevis bloom in 2000 produced indeterminate results. Admittance of cormorants with outbreak-specific clinical signs was positively correlated (P < 0.05) with concurrent concentrations of K. brevis in local water. The cross-correlation coefficient was also significant when increased K. brevis levels preceded cormorant admittances by 2, 4, 6, and 8 wk. This delay in time between K. brevis blooms and cormorant admittance and our clinical finding of neurologic abnormalities in cormorants without overt histopathologic features suggest an association between K. brevis blooms and local cormorant morbidity.  相似文献   

Reproductive medicine in koi (Cyprinus carpio).     

Gregory A Lewbart 《Veterinary Clinics of North America: Exotic Animal Practice》2002,5(3):637-48, vii

Each year more veterinarians are seeing ornamental fish patients and our knowledge of how to manage the pet fish patient is increasing at a rapid pace. A large percentage of these patients are nishikigoi (Cyprinus carpio) which are taxonomically an ornamental carp. In this country and in many parts of the world we refer to these fish as koi. Reproductive medicine problems are well documented in koi and a thorough knowledge of this subject will aid the fish practitioner in diagnosis and treatment.  相似文献   

The Fear of Specialization     

Steven L.  Peterson DVM  MD 《Veterinary surgery : VS》1997,26(6):502-502

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慎入美利坚之"门"     

GregoryFrumkin 《国际木业》2003,33(9):3-4

美国的木门市场划分为2个主要类型(CATBGORES)。一种是空心门，每年销售4300万扇(其中超过2000万扇是模压纤维板门皮)，用于住宅市场。另一种是室内镶板木门，在美国每年安装500万扇。  相似文献