Pomegranate Juice and Peel Extracts are Able to Inhibit Proliferation,Migration and Colony Formation of Prostate Cancer Cell Lines and Modulate the Akt/mTOR/S6K Signaling Pathway     

Chaves  Fernanda Machado  Pavan  Isadora Carolina Betim  da Silva  Luiz Guilherme Salvino  de Freitas  Lidia Broglio  Rostagno  Mauricio Ariel  Antunes  Adriane Elisabete Costa  Bezerra  Rosângela Maria Neves  Simabuco  Fernando Moreira 《Plant foods for human nutrition (Dordrecht, Netherlands)》2020,75(1):54-62

Plant Foods for Human Nutrition - Pomegranate (Punica granatum) is known to contain polyphenols with many potential health benefits, including anti-tumoral, anti-inflammatory, and anti-microbial...  相似文献   

Assessing Relationships Between Genetic Evaluations Using Robust Regression with an Application to Holsteins in Uruguay     

Guilherme J. M. Rosa  Daniel Gianola  Jorge I. Urioste 《Acta Agriculturae Scandinavica, Section A - Animal Sciences》2013,63(1):21-34

Globalization of dairy cattle breeding has created a need for international sire proofs. Some early methods for converting proofs from one population to another are based on simple linear regression. An alternative robust regression method based on the t-distribution is presented, and maximum likelihood and Bayesian techniques for analysis are described, including the situation in which some proofs are missing. Procedures were used to investigate the relationship between Holstein sire proofs obtained by two Uruguayan genetic evaluation programs. The results suggest that conversion equations developed from data including only sires having proofs in both populations can lead to distorted results, relative to estimates obtained using techniques for incomplete data. There was evidence of non-normality of regression residuals, which constitutes an additional source of bias. A robust estimator may not solve all problems, but can provide simple conversion equations that are less sensitive to outlying proofs and to departures from assumptions.  相似文献   

Isolation and partial characterization of a pectic polysaccharide from the fruit pulp of Spondias cytherea and its effect on peritoneal macrophage activation     

Iacomini M  Serrato RV  Sassaki GL  Lopes L  Buchi DF  Gorin PA 《Fitoterapia》2005,76(7-8):676-683

The total carbohydrate content of the intact pulp of Spondias cytherea was 41%. Polysaccharides were obtained via hot aqueous extraction after defatting with organic solvents. The aqueous extract was treated with excess ethanol to form a precipitate, which was then solubilized in water. The material precipitated upon acidification when HCl was removed. The resulting supernatant fraction was submitted to freeze-thawing treatment yielding a soluble fraction (sFTS). This fraction had Ara, Rha, Gal and GalA in its structure as determined by GC-MS. 13C NMR analysis showed signals assigned to alpha-L-Araf, beta-D-Galp, alpha-D-GalpA and alpha-L-Rhap units, in addition to galacturonic acid units, which were present also as methyl ester. These results suggest a type I rhamnogalacturonan with arabinogalactan branches. Cell eliciting activity in a dose-depending pattern was observed in vitro on peritoneal macrophages treated with sFTS.  相似文献   

Antitumor activity of three benzopyrans isolated from Hypericum polyanthemum     

Ferraz AB  Grivicich I  von Poser GL  Faria DH  Kayser GB  Schwartsmann G  Henriques AT  da Rocha AB 《Fitoterapia》2005,76(2):210-215

In the present study we have investigated the in vitro antitumor effects of three benzopyrans, 6-isobutyryl-5,7-dimethoxy-2,2-dimethylbenzopyran (1), 7-hydroxy-6-isobutyryl-5-methoxy-2,2-dimethylbenzopyran (2) and 5-hydroxy-6-isobutyryl-7-methoxy-2,2-dimethylbenzopyran (3) isolated from Hypericum polyanthemum. The three compounds tested demonstrated potent growth inhibitory activity at 40 microg/ml (<25% control growth) in the NCI-H460, HT-29 and U-373MG human cell lines. Determination of cell cycle distribution demonstrated that the antiproliferative effect of the three benzopyrans could be associated to alterations in the cell cycle phase distribution. Treatment with the IC50 of the three compounds induced an arrested in S phase. Only in cells treated with compound 3 did the percentage of sub-G1 population increase up to 9%, suggesting that this compound induced more cell death than the others. Consistent with sub-G1 analysis, appreciable oligonucleosomal DNA fragmentation was only observed in the NCI-H460 cell line treated with compound 3. From these results it can be suggested that despite no differences among the cytotoxicity of the three compounds, it was observed that the mechanism of their antiproliferative effects appears to be different.  相似文献   

Assisted Selection by Specific DNA Markers for Genetic Elimination of the Kunitz Trypsin Inhibitor and Lectin in Soybean Seeds     

Rita Maria Alves de Moraes  Tais Cristina Bastos Soares  Lucinete Regina Colombo  Maria Fernanda Spegiorin Salla  Josie Gomes de Almeida Barros  Newton Deniz Piovesan  Everaldo Gonçalves de Barros  Maurilio Alves Moreira 《Euphytica》2006,149(1-2):221-226

Summary The antinutritional factors found in soybean, lectin (SBL) and Kunitz trypsin inhibitor (SKTI), are usually inactivated by heat treatment. However, residual activity of these factors can be found in several types of soy-derived products. Heat treatment does not completely eliminate these factors, and in addition it may considerably decrease protein solubility. The genetic elimination of these antinutritional factors could be an alternative to the heat treatment. This study aimed to develop soybean lines devoid of SKTI and SBL in their seeds. The population under study was obtained by crossing the normal cv. Monarca with a soybean line lacking SKTI and SBL. Specific DNA primers were designed for the identification of the recessive alleles that condition the absence of SKTI and SBL. F₂ seeds presenting the DNA markers that identify the recessive alleles were selected and the corresponding F₂ plants were backcrossed with the recurrent parent (‘Monarca’), producing the BC₁F₁ generation. The F₂ generation was analyzed by SDS-PAGE in order to confirm the genotypes of the F₂ selected. The segregation tests confirmed that these traits are governed by two genes that segregate independently.  相似文献   

Correlation of testicular artery Doppler velocimetry with kinetics and morphologic characteristics of epididymal sperm in dogs     

Luiz Guilherme Corsi Trautwein  Anne Kemmer Souza  Guilherme Schiess Cardoso  Karina Keller Marques da Costa Flaiban  Antonio Cezar de Oliveira Dearo  Maria Isabel Mello Martins 《Reproduction in domestic animals》2020,55(6):720-725

Sperm quality can be affected by a reduction in testicular blood flow, which can be measured by Doppler ultrasonography. The aim of this study was to correlate the Doppler velocimetry of the testicular artery with kinetics of the epididymal spermatozoa in dogs. Twenty-two dogs (44 testicles) were evaluated by Doppler ultrasonography in five regions of the testicular artery before orchiectomy. Spermatozoa were recovered by the epididymal tail compression technique and analysed for kinetics on a computer-assisted semen analysis (CASA system). Morphology (modified Karras) and sperm membrane integrity were analysed by eosin–nigrosine staining. Data were analysed by Pearson's correlation test (p < .01). The mean total motility was 69.0% ± 17.7, progressive motility was 43.7% ± 14.7, average path velocity (VAP) was 127.0 µm/s ± 20.7, curvilinear velocity (VCL) was 221.0 µm/s ± 31.1, and sperm velocity index (SVI) was 389.9 ± 56.1. There were positive correlations between the peak systolic velocity (PSV) in the proximal supratesticular region with the SVI (r = .529), VCL (r = .555) and VAP (r = .473), and a negative correlation with the percentage of slow spermatozoa (r = −.463). The results suggest that the testicular artery blood flow velocity can positively affect the speed of spermatozoa movement. For the first time, we have correlated sperm kinetics with the Doppler evaluation of the testicular artery in dogs.  相似文献   

Testing a novel spatially-explicit dynamic modelling approach in the scope of the laurel forest management for the endangered Azores bullfinch (Pyrrhula murina) conservation     

Rita Bastos  Mário Santos  Jaime Albino Ramos  Joana Vicente  Carlos Guerra  Joaquim Alonso  João Honrado  Ricardo Santos Ceia  Sérgio Timóteo  João Alexandre Cabral 《Biological conservation》2012,147(1):243-254

The Azores bullfinch (Pyrrhula murina) is an endemic bird of São Miguel island (Azores Archipelago, Portugal), currently threatened by two of the major causes of biodiversity loss worldwide: invasion of native habitats by exotic plants and habitat destruction by land use changes. The aim of this research was to develop and test a novel spatially explicit modelling framework that predicts the Azores bullfinch responses to alternative realistic scenarios of native forest management. This was done by integrating Multi-Model Inference statistical analysis, Stochastic-Dynamic Modelling and Geographic Information Systems under a common framework relating bird population trends to changes in the surrounding habitats. Overall, in the next 25 years, the Azores bullfinch breeding population was predicted to increase around 19% as a consequence of habitat management actions already implemented (“LIFE Priolo” project) or around 27% in the context of realistic future habitat restoration scenarios. These results represent, respectively, a supplementary increase of more 6% or more 13% in the Azores bullfinch abundance when compared with the trends simulated for the scenario without management. Nevertheless, those actions seemed to be relatively ineffective in promoting the expansion of the species from the actual restricted favourable area, essentially due to local forestry dynamics and on-going plant invasion processes. This novel integrative approach provides a promising baseline to support ecological models with increased realism and predictive power, making the outputs more useful and intuitive to decision-makers and environmental managers.  相似文献   

The impact of redox conditions on the rare earth element signature of redoximorphic features in a soil sequence developed from limestone     

Cédric Laveuf  Sophie Cornu  Luiz Roberto G. Guilherme  Annie Guerin  Farid Juillot 《Geoderma》2012

Redox processes, which are widespread in soils, need to be quantified for an improved comprehension of the dynamics of Fe- and Mn-oxides and their associated trace elements. The classical methodology used to study these redox processes generally relies on the quantification of all mineral species in the various pedological features that can be related to different redox stages. However, this approach usually encounters the difficulty of precisely quantifying the different forms of poorly crystallised Fe- and Mn-oxides.In this study, we use the signature of rare earth elements (REEs) to visualise and, eventually, quantify the importance of redox processes in soils. Our approach relies on that developed by Laveuf et al. (2008) and the idea that the relative contribution to the mobilisation of REEs that is made by the primary minerals reactive to redox conditions depends on the following factors: (i) their initial proportion in the different pedological features that can be related to various redox processes, (ii) their relative mobilisation during the redox process in question, and (iii) their initial REE signatures.The catena studied is characterised by two stages of redox conditions: the first is related to the formation and subsequent dissolution of Fe–Mn concretions, and the second is related to the bleaching of the soil matrix due to morphological degradation. In this soil, the main minerals reactive to redox conditions are Mn-oxides, ferrihydrite, goethite and (fluor)apatite. The results indicate that the primary redox conditions can be characterised by a positive Ce anomaly on the REE pattern, which has been attributed to a preferential immobilisation of this element, due to its association with Mn-oxides. The results also indicate that the secondary redox conditions can be characterised by depletion in medium REEs (MREEs) in the REE pattern, which has been attributed to a preferential release of these elements during the dissolution of (fluor)apatite and, to a lesser extent, of ferrihydrite.These results emphasise the potential of REE signatures of the visualisation of the various redox processes that have been active in a soil. Additionally, REE signatures are a proxy of the frequency and intensity of the redox conditions.  相似文献   

Antiviral Sulfoquinovosyldiacylglycerols (SQDGs) from the Brazilian Brown Seaweed Sargassum vulgare     

Erwan Plouguerné  Lauro M. de Souza  Guilherme L. Sassaki  Jéssica Figueiredo Cavalcanti  Maria Teresa Villela Romanos  Bernardo A. P. da Gama  Renato Crespo Pereira  Eliana Barreto-Bergter 《Marine drugs》2013,11(11):4628-4640

Total lipids from the Brazilian brown seaweed Sargassum vulgare were extracted with chloroform/methanol 2:1 and 1:2 (v/v) at room temperature. After performing Folch partition of the crude lipid extract, the lipids recovered from the Folch lower layer were fractionated on a silica gel column eluted with chloroform, acetone and methanol. The fraction eluted with methanol, presented a strong orcinol-positive band characteristic of the presence of sulfatides when examined by TLC. This fraction was then purified by two successive silica gel column chromatography giving rise to fractions F4I86 and F4II90 that exhibited strong activity against herpes simplex virus type 1 and 2. The chemical structures present in both fractions were elucidated by ESI-MS and ¹H/¹³C NMR analysis HSQC fingerprints based on their tandem–MS behavior as sulfoquinovosildiacylglycerols (SQDGs). The main SQDG present in both fractions and responsible for the anti-herpes activity observed was identified as 1,2-di-O-palmitoyl-3-O-(6-sulfo-α-d-quinovopyranosyl)-glycerol.  相似文献   

Meta-analysis of the prevalence and risk factors associated with bovine neosporosis     

Ribeiro  Claudia Mello  Soares  Isabela Ribeiro  Mendes  Rodrigo Guerrero  de Santis Bastos  Paula Andrea  Katagiri  Satie  Zavilenski  Renato Bacarin  de Abreu  Hudson Felipe Porto  Afreixo  Vera 《Tropical animal health and production》2019,51(7):1783-1800

Tropical Animal Health and Production - Neospora caninum is one of the main infectious agents that cause reproductive disorders in cattle. However, knowledge about the prevalence and causal factors...  相似文献