Potato quality XIX: A preliminary study of the Browning reaction in potato chips using radiophosphorus     

Thomas G. Hart  Ora Smith 《American Journal of Potato Research》1963,40(12):421-429

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Real-time polymerase chain reaction monitoring of recombinant DNA entry into soil from decomposing roundup ready leaf biomass     

Levy-Booth DJ  Campbell RG  Gulden RH  Hart MM  Powell JR  Klironomos JN  Pauls KP  Swanton CJ  Trevors JT  Dunfield KE 《Journal of agricultural and food chemistry》2008,56(15):6339-6347

Glyphosate-tolerant, Roundup Ready (RR) soybeans account for about 57% of all genetically modified (GM) crops grown worldwide. The entry of recombinant DNA into soil from GM crops has been identified as an environmental concern due to the possibility of their horizontal transfer to soil microorganisms. RR soybeans contain recombinant gene sequences that can be differentiated from wild-type plant and microbial genes in soil by using a sequence-specific molecular beacon and real-time polymerase chain reaction (PCR). A molecular beacon-based real-time PCR system to quantify a wild-type soybean lectin ( le1) gene was designed to compare amounts of endogenous soybean genes to recombinant DNA in soil. Microcosm studies were carried out to develop methodologies for the detection of recombinant DNA from RR soybeans in soil. RR soybean leaf litterbags were imbedded in the soil under controlled environmental conditions (60% water holding capacity, 10/15 degrees C, and 8/16 h day/night) for 30 days. The soybean biomass decomposition was described using a single-phase exponential equation, and the DNA concentration in planta and in soil was quantified using real-time PCR using sequence-specific molecular beacons for the recombinant cp4 epsps and endogenous soybean lectin ( le1) genes. The biomass of RR soybean leaves was 8.6% less than nontransgenic (NT) soybean leaves after 30 days. The pooled half-disappearance time for cp4 epsps and le1 in RR and of le1 in NT soybean leaves was 1.4 days. All genes from leaves were detected in soil after 30 days. This study provides a methodology for monitoring the entry of RR and NT soybean DNA into soil from decomposing plant residues.  相似文献   

The importance of demographic niches to tree diversity   总被引：1，自引：0，他引：1  

Condit R  Ashton P  Bunyavejchewin S  Dattaraja HS  Davies S  Esufali S  Ewango C  Foster R  Gunatilleke IA  Gunatilleke CV  Hall P  Harms KE  Hart T  Hernandez C  Hubbell S  Itoh A  Kiratiprayoon S  Lafrankie J  de Lao SL  Makana JR  Noor MN  Kassim AR  Russo S  Sukumar R  Samper C  Suresh HS  Tan S  Thomas S  Valencia R  Vallejo M  Villa G  Zillio T 《Science (New York, N.Y.)》2006,313(5783):98-101

Most ecological hypotheses about species coexistence hinge on species differences, but quantifying trait differences across species in diverse communities is often unfeasible. We examined the variation of demographic traits using a global tropical forest data set covering 4500 species in 10 large-scale tree inventories. With a hierarchical Bayesian approach, we quantified the distribution of mortality and growth rates of all tree species at each site. This allowed us to test the prediction that demographic differences facilitate species richness, as suggested by the theory that a tradeoff between high growth and high survival allows species to coexist. Contrary to the prediction, the most diverse forests had the least demographic variation. Although demographic differences may foster coexistence, they do not explain any of the 16-fold variation in tree species richness observed across the tropics.  相似文献   

Factors affecting colostrum quality in Australian pasture‐based dairy herds          下载免费PDF全文

GM Chuck  PD Mansell  MA Stevenson  MM Izzo 《Australian veterinary journal》2017,95(11):421-426

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Application of compost and clay under water-stressed conditions influences functional diversity of rhizosphere bacteria     

Bede?S.?MickanEmail author  Lynette?K.?Abbott  Jingwei?Fan  Miranda?M.?Hart  Kadambot?H.?M.?Siddique  Zakaria?M.?Solaiman  Sasha?N.?Jenkins 《Biology and Fertility of Soils》2018,54(1):55-70

Applications of compost and clay to ameliorate soil constraints such as water stress are potential management strategies for sandy agricultural soils. Water repellent sandy soils in rain-fed agricultural systems limit production and have negative environmental effects associated with leaching and soil erosion. The aim was to determine whether compost and clay amendments in a sandy agricultural soil influenced the rhizosphere microbiome of Trifolium subterraneum under differing water regimes. Soil was amended with compost (2% w/w), clay (5% w/w) and a combination of both, in a glasshouse experiment with well-watered and water-stressed (70 and 35% field capacity) treatments. Ion Torrent 16S rRNA sequencing and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis of functional gene prediction were used to characterise the rhizosphere bacterial community and its functional component involved in nitrogen (N) cycling and soil carbon (C) degradation. Compost soil treatments increased the relative abundance of copiotrophic bacteria, decreased labile C and increased the abundance of recalcitrant C degrading genes. Predicted N cycling genes increased with the addition of clay (N₂ fixation, nitrification, denitrification) and compost + clay (N₂ fixation, denitrification) and decreased with compost (for denitrification) amendment. Water stress did not alter the relative abundance of phylum level taxa in the presence of compost, although copiotrophic Actinobacteria increased in relative abundance with addition of clay and with compost + clay. A significant role of compost and clay under water stress in influencing the composition of rhizosphere bacteria and their implications for N cycling and C degradation was demonstrated.  相似文献   

Browsing by fallow deer (Dama dama) in young broadleaved plantations: seasonality, and the effects of previous browsing and bud eruption     

Moore  N.P.; Hart  J.D.; Kelly  P.F.; Langton  S.D. 《Forestry》2000,73(5):437-445

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Pb isotopic variability in melt inclusions from oceanic island basalts, polynesia     

AE Saal  SR Hart  N Shimizu  EH Hauri  GD Layne 《Science (New York, N.Y.)》1998,282(5393):1481-1484

Previous studies have suggested that melting processes are responsible for the trace element variability observed in olivine-hosted basaltic melt inclusions. Melt inclusions from three individual lava samples (two from Mangaia, Cook Islands, and one from Tahaa, Society Islands) have heterogeneous Pb isotopic compositions, even though the erupted lavas are isotopically homogeneous. The range of Pb isotopic compositions from individual melt inclusions spans 50 percent of the worldwide range observed for ocean island basalts. The melt inclusion data can be explained by two-component mixing for each island. Our data imply that magmas with different isotopic compositions existed in the volcanic plumbing system before or during melt aggregation.  相似文献   

Gold nanoelectrodes of varied size: transition to molecule-like charging   总被引：1，自引：0，他引：1  

S Chen  RS Ingram  MJ Hostetler  JJ Pietron  RW Murray  TG Schaaff  JT Khoury  MM Alvarez  RL Whetten 《Science (New York, N.Y.)》1998,280(5372):2098-2101

A transition from metal-like double-layer capacitive charging to redox-like charging was observed in electrochemical ensemble Coulomb staircase experiments on solutions of gold nanoparticles of varied core size. The monodisperse gold nanoparticles are stabilized by short-chain alkanethiolate monolayers and have 8 to 38 kilodaltons core mass (1.1 to 1.9 nanometers in diameter). Larger cores display Coulomb staircase responses consistent with double-layer charging of metal-electrolyte interfaces, whereas smaller core nanoparticles exhibit redox chemical character, including a large central gap. The change in behavior is consistent with new near-infrared spectroscopic data showing an emerging gap between the highest occupied and lowest unoccupied orbitals of 0.4 to 0.9 electron volt.  相似文献   

Fishes of the Open Ocean: A Natural History and Illustrated Guide     

Paul J B Hart 《Fish and Fisheries》2011,12(4):471-472

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Land restoration management after topsoil mining and implications for restoration policy guidelines in New Zealand     

P. B. S. Hart  A. W. West  J. A. Kings  H. M. Watts  J. C. Howe 《Land Degradation \u0026amp; Development》1999,10(5):435-453

A three-year field trial on an upland loessial soil (Belmont silt loam) in New Zealand investigated the effects of ripping, application of fertilizer N and grazing management on the recovery of some physical, chemical and biochemical properties of soil and pasture productivity following removal (mining) of topsoil. Removing the top 31 cm of soil by mining (all of the A horizon and part of the AB horizon) also removed most of the soil's labile organic matter fractions, and to a lesser extent its total organic matter. After three years, the microbial C and mineralizable N in the 0–10 cm depth of mined soil had reached 65 and 62 per cent of the corresponding levels in unmined soil. Ripping to a depth of 30 cm, application of fertilizer N and lenient grazing of the pasture failed to enhance the recovery of soil fertility. A soil sampling depth of 20 cm provided a reasonable basis to assess the microbial biomass and potential fertility. Pasture productivity was, on average, 30 per cent lower on mined land than on unmined land over the first three years after mining. Application of N proved uneconomic in terms of farm production, although a good pasture response to N fertilizer was obtained. The results from this and related trials are summarized as a series of recommendations for the monitoring and regulation of topsoil mining. An incentive regime is also recommended to encourage land owners and/or topsoil miners to use successful restoration techniques on topsoil-mined land. Copyright © 1999 John Wiley & Sons, Ltd.  相似文献