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31.
Antifungal and antitermitic activities of wood vinegar produced from Vitex pubescens were evaluated. Three kinds of wood vinegar were produced at three different pyrolysis temperatures, i.e. at 350, 400 and 450 °C. A PDA dilution method was employed to assay antifungal activity of the vinegars with a white-rot fungus Trametes versicolor and a brown-rot fungus Fomitopsis palustris. Termiticidal activity and repellent effect were evaluated by a no-choice test and a choice test with Reticulitermes speratus and Coptotermes formosanus. All wood vinegars exhibited antifungal activity against both fungi. Wood vinegar of 450 °C had the higher activity than those of 400 and 350 °C. It was assumed that acid component contributed to the increase in controlling the growth of fungal. The wood vinegar exhibited antitermite activity to both R. speratus and C. formosanus workers in the no-choice experiment. However, it needed relatively high concentration to obtain the perfect mortality. For instance, the wood vinegar of 10 % concentration was needed to achieve 100 % mortality against C. formosanus, whereas for R. speratus only 3 % of wood vinegar was required. In the direct-choice experiment, wood vinegar had a significantly repellent effect to both termites at the lowest treating concentration of 10 %.  相似文献   
32.

Background

Factor XI (FXI) is a plasma protein that participates in the formation of blood clots. Factor XI deficiency is autosomal recessive hereditary disorder that may be associated with excess bleeding in Holstein cattle.

Methods

In this study, 225 Holstein cows reared in Turkey were screened in order to identify FXI genotypes. DNA extractions were obtained from the fresh blood of the cows. Amplicons of FXI exon 12 were obtained by Polymerase Chain Reaction (PCR), and analyzed by 2% agarose gel electrophoresis stained with ethidium bromide. Additionally, all cows were confirmed by DNA sequencing to determine whether or not there was a mutant allele.

Results

Carriers of the FXI deficiency have two DNA fragments of 320 bp and 244 bp in size. The results of our study demonstrated that only four out of the 225 Holstein cows tested in Turkey carried the FXI deficiency. The frequency of the mutant FXI allele and the prevalence of heterozygous cows were found as 0.9% and 1.8%, respectively.

Conclusion

The DNA-based test determines all genotypes, regardless of phenotype or FXI activity. The mutation responsible for the FXI deficiency had not been detected in Holstein cattle in Turkey before prior to this study. The frequency of the mutant FXI allele needs to be confirmed by carrying out further analyses on cattle in Turkey and the selection programs should be developed to eliminate this genetic disorder.  相似文献   
33.
Congenital malformations with multiple anomalies have been described infrequently in the veterinary literature. A stillborn male crossbred lamb with diprosopus, craniorachischisis, and arthrogryposis was examined macroscopically and histopathologically in this study. The left head was smaller than the right head. Micrencephaly, agnathia, and a rudimentary tongue, which was adherent to the palate, were present in the left head. Micrencephaly, brachygnathia superior, and cleft palate were present in the right head. Cerebellar agenesis and spinal cord hypoplasia were observed. The cerebrums and the spinal cord were covered with a tapering membranous structure. Neural and dermal tissues were noted to intervene upon microscopic examination of this structure. Disorganization of neurons was observed in both cerebrums, though it was more severe in the left one. This case demonstrates many congenital defects occurring together in a lamb.  相似文献   
34.
The aim of this study was to determine the serum concentrations, ovarian presence and expression of anti‐Müllerian hormone (AMH) in pre‐pubertal, bitches with signs of ovarian remnant syndrome (ORS) and intact bitches. In addition, we aimed to verify the suitability of serum AMH concentrations for diagnostic purposes in sterilized bitches and/or in suspected cases of ORS in the field of veterinary medicine. For this purpose, 36 healthy female dogs divided into six groups: proestrus, oestrus, dioestrus, anoestrus, pre‐pubertal and ORS. Serum AMH concentrations were determined by electrochemiluminescence immunoassay, and ovarian presence and distribution of AMH was confirmed by immunohistochemical and qPCR techniques. According to the results of qPCR, while the expression values of AMH were at the highest concentrations in the proestrus and oestrus, there was a statistically significant decrease in these values at the later stages of the cycle (p < 0.05). According to hormone analysis, the serum AMH values of the ORS group had decreased significantly compared with the proestrus and oestrus (p < 0.05). Although serum AMH levels of ORS group were increased compared with anestrus and pre‐pubertal groups, this increase was statistically non‐significant (p > 0.05). Immunohistochemically, AMH expression was first observed in the granulosa cells of primordial follicles in folliculogenesis. Expression values were the highest in the proestrous and oestrus groups, but values from bitches in later stages of the cycle were statistically significant decrease in comparison with these groups (p < 0.05). As a result, AMH concentration and expression were found to be higher in proestrus and oestrus than in other periods (p < 0.05). In addition, the measurable level of AMH concentration in bitches with ORS is an indication that it can be used in the diagnosis of ORS.  相似文献   
35.
36.
The present study was conducted in order to determine the pomological and morphological characteristics and chemical compositions of some local apple varieties grown in Gumushane. As a result of field work in Gumushane province, sixteen different apples, which are Summer Apple, English apple, Green Belly, Black Belly and Yellow Belly Apples, Amasya Marble, Bey Apple, Chest Apple, Mahsusa, Arabian Girl, Willow Apple, Tav?anba??, Yellow H?d?r, Local Marble, Bride Apple, and Fatty Apple were collected in order to analyze. The average results of pomological and morphological properties and chemical compositions were obtained as following: fruit mass; 127.36 g, fruit width; 65.23 mm, fruit length; 57.30 mm, fruit stalk thickness; 2.17 mm, fruit stalk length; 17.63 mm, stalk pit depth; 12.75 mm, flower pit depth; 10.29 mm, fruit firmness; 13.79 kg/cm2, seed house width; 15.89 mm, seed house length; 17.39 mm, number of seeds; 4.3, water soluble dry matter content; 14.53?%, ash content; 1.11?%, pH; 3.8, titratable acidity; 0.69 g/L, water content; 83.8?%, reducing sugar; 5.04 g/100 g, sucrose; 2.25 g/100 g, and the amount of total sugars; 7.29 g/100. The fruit peel color was determined by considering the light transmittance (L). The average L values were ranged between 112.06 and 66.32. On the other hand among the mineral elements, potassium and manganese amounts were determined as the highest and the lowest, respectively.  相似文献   
37.
Genetic diversity for micronutrient efficiency among the most highly adapted and advanced hexaploid and tetraploid wheat cultivars in the world is limited compared with alien species of wheat or rye. Therefore, screening for zinc efficiency was conducted in greenhouse experiments under controlled conditions, and in field trials. Different varieties of hexaploid wheat, hexaploid oats and diploid rye, together with hexaploid and octoploid triticales, wheat-Agropyron, wheat-Aegilops and several wheat-alien chromosome addition series were studied. Considerable differences in zinc efficiency were found between wheat and its relatives. Individual chromosomes of Secale, Agropyron and Haynaldia were found to carry major genes for this character. The transfer of alien chromosome segments was effective, demonstrated using several wheat-rye translocation lines. Alien genetic information was clearly expressed in the wheat genetic background. Further experimental introgressions by chromosome manipulation and marker-aided selection may efficiently contribute to wheat improvement in marginal soils. This revised version was published online in August 2006 with corrections to the Cover Date.  相似文献   
38.
Tilapia lake virus (TiLV) is an emerging pathogen in aquaculture, reportedly affecting farmed tilapia in 16 countries across multiple continents. Following an early warning in 2017 that TiLV might be widespread, we executed a surveillance programme on tilapia grow-out farms and hatcheries from 10 districts of Bangladesh in 2017 and 2019. Among farms experiencing unusual mortality, eight out of 11 farms tested positive for TiLV in 2017, and two out of seven tested positive in 2019. Investigation of asymptomatic broodstock collected from 16 tilapia hatcheries revealed that six hatcheries tested positive for TiLV. Representative samples subjected to histopathology confirmed pathognomonic lesions of syncytial hepatitis. We recovered three complete genomes of TiLV from infected fish, one from 2017 and two from 2019. Phylogenetic analyses based on both the concatenated coding sequences of 10 segments and only segment 1 consistently revealed that Bangladeshi TiLV isolates formed a unique cluster within Thai clade, suggesting a close genetic relation. In summary, this study revealed the circulation of TiLV in 10 farms and six hatcheries located in eight districts of Bangladesh. We recommend continuing TiLV-targeted surveillance efforts to identify contaminated sources to minimize the countrywide spread and severity of TiLV infection.  相似文献   
39.
Agroforestry is one of the most sustainable land management systems practiced around the world due to the socioeconomic benefits that it brings to farmers. In Bangladesh, farmers practice agroforestry, applying indigenous knowledge. The present study was designed to identify the present status, management practices and its role in improving the livelihoods of farmers in northern Bangladesh. Data for the study were collected through quantitative and qualitative methods. A total of 29 tree and 38 agricultural crop species were planted by the102 farmers interviewed. Mangifera indica (relative prevalence 49%) is the most predominant species, followed by Eucalyptus camaldulensis (relative prevalence 35.4%). Farmers of northern Bangladesh plant trees in cropland for fruits (90%), fuel wood (87%) and timber production (79%). Fruit trees were planted with wider spacing while forest and fuel wood species were planted with narrower spacing. Farmer’s livelihoods improved enormously by practicing agroforestry as they have more access to food, fodder and fuel wood which is reflected by greater access to livelihood capitals (except social capital). However, farmers have experienced increased incidences of pests and diseases to the annual crops and trees. Agroforestry practices increases species diversity, ensure economic return and sustain farmer’s livelihoods.  相似文献   
40.
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