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Molecular diversity and development of the Lactobacillus community in the intestinal tract, as influenced by age and intestinal compartment, were studied in one litter of 12 conventionally raised piglets. Piglets were euthanized at each week (3 animals per time). Digesta and tissue samples from stomach, duodenum, jejunum, ileum, caecum, colon, and rectum were collected and analysed by using 16S ribosomal RNA-based methods. DGGE (denaturing gradient gel electrophoresis)profiles revealed that the Lactobacillus communities throughout the GI tract from duodenum to rectum showed good stability at same age. This indicates that fecal Lactobacillus communities can effectively represent the intestinal community.Two dominant bands were found in tissue samples of the small intestine, suggesting that the lactobacilli can adhere to the small intestinal wall. The Lactobacillus communities in different GI tract compartments developed over time. A successional change of Lactobacillus communities was observed from birth, through creep feeding to one week after weaning, showing a trend from simple to complex and back to simple. Furthermore, a clone library of Lactobacillus spp. 16S rRNA gene sequences were generated from jejunal and colonic thymes. Six dominant DGGE bands generated from jejunal chymes were matched with sequences that show 94-98% similarity to the bands derived from L. reuteri, L. delbrueckii, and L. crispatus. Seven dominant DGGE bands generated from colon chymes were matched with sequences that show 88-99% similarity to those derived from L. reuteri, L. delbrueckii, L. amylovorus/L, sobrius, and L. acidophilus. Amplicons related to L. reuteri were found in all DGGE fingerprints from jejunal digesta of age of weeks 1, 3, and 4. Amplicons related to L. amylovorus/L, sobrius were present in all DGGE fingerprints from colonic digesta of age of week 1, 3, and 4. Amplicons related to L. delbrueckii were found before weaning, L. crispatus after creep feeding before weaning and L. acidophilus after weaning. This indicates that L. reuteri and L. amylovorus/L, sobrius probably belong to the permanent composition,while L. delbruckii, L. acidophilus, and L. crispatus probably belong to the temporal groups of Lactobacillus communities in the GI tract of piglets. 相似文献
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Di Jin Hauke L. Kite-Powell Porter Hoagland 《Aquaculture Economics & Management (Blackwell Science)》2013,17(3):225-242
In assessing the future expansion of aquaculture in coastal-ocean environments, most studies focus only on the constraint posed by the local environmental assimilative capacity. In open-ocean environments, however, the assimilative capacity is difficult to gauge. We develop an alternative economic approach for projecting the growth of the open-ocean aquaculture industry. We evaluate equilibria in the market for seafood, where the product may be supplied either by a wild-harvest fishery or open-ocean aquaculture or both. In our framework, the net demand for farmed fish determines the size of the aquaculture industry and, in turn, the levels of pollution discharges. We illustrate our approach with a case study of a groundfish fishery and the proposed open-ocean aquaculture of Atlantic cod in New England. We find that, for a range of competitive production costs for aquaculture, the optimal industry structure would comprise both a wild-harvest fishery and aquaculture. 相似文献
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Hauke L. Kite-Powell Michael C. Rubino Bruce Morehead 《Aquaculture Economics & Management (Blackwell Science)》2013,17(3):228-250
The United States today imports most of the seafood it consumes. Half of these imports are from aquaculture. Domestic wild capture production is limited and U.S. aquaculture production has declined in recent years. Policy, socioeconomic, and regulatory obstacles stand in the way of expanded U.S. aquaculture production. In this article, we examine the implications of two future paths for seafood supply: an increasing reliance on imports, and a shift toward increased domestic aquaculture production. We examine global trends, likely future developments in U.S. seafood demand and supply, and implications of the path of U.S. aquaculture development for U.S. seafood supply and prices, employment, ecological footprint, and seafood supply security and safety. We conclude with recommendations for a path forward that serves the interests of the nation and the global community in the search for economically sound and sustainable ways to feed a growing population. 相似文献
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We compared the abundance and diversity of cultivable protozoa (flagellates and amoebae) in a polycyclic aromatic hydrocarbon (PAH) polluted soil and an unpolluted control, by isolating and cultivating clonal strains. The number of cultivable protozoa was higher in the polluted soil; however, the polluted soil displayed an impoverished community, dominated by certain taxa, such as Acanthamoeba sp. We isolated a total of 31 protozoan strains to characterize them morphologically and by 18S rRNA gene sequence analysis. This approach, i.e. combining morphological and molecular information had the advantage of providing quantitative data, information on morphology but also an accurate positioning of the isolates in 18S rRNA trees. 相似文献
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Applicability of geophysical prospecting methods for mapping of soil compaction and variability of soil texture on farm land The increasing degree of mechanization in agriculture has resulted in the use of more powerful and heavier tractors and machines. Consequently, mechanical burden to soils has increased, too, which can lead to persistent subsoil compaction at depths below 30 cm. In soils damaged by compaction soil functions like transportation of water and air decrease. Because of that, conditions for plant growth are getting worse and the soils' natural regulation functions could be impaired. In order to take counteractive measures, it is necessary to get information about the status of soil compaction. Up to now, the status of soil compaction can only be determined at single points in laboratory measurements or with less accuracy in field measurements. Therefore, the demand for an efficient planar‐mapping system arises. The applicability of different geophysical prospecting methods with regard to this problem has been examined. For this purpose, geophysical and soil measurements were performed in a field with conventional agricultural land use in Schleswig‐Holstein (Germany) on a young moraine site. We applied GPR (Ground Penetrating Radar) with main frequencies 500 MHz and 900 MHz, supplemented by inductive electromagnetic technique (EM) using the Ground Conductivity Meter EM38 and high‐resolution refraction seismic using compressional and shear waves. Differences in soil type were found by all these geophysical methods and confirmed by soil measurements, therefore, locations with higher risk for compaction (loamy soils) could be distinguished from locations with lower risk (sandy soils). Under humid conditions, radar data showed strong reflections at a depth of approx. 30 cm. During summer, under dry conditions, these reflections did not occur. This temporal variation of radar reflections can be explained by variable water layers inside the soil, which can be regarded as an indicator for compacted soil. The seismic investigation was performed along short (12 m) profiles with dense (20 cm) sensor spacing. Excellent data quality showed that this sort of measurement, known from engineering geophysics, is also feasible for soil investigations. We performed both compressional‐ (P‐) and shear‐(SH‐) wave refraction studies. Differences in soil type of subsoil affected especially seismic velocities of P‐waves. Whether or not areas of compacted soil can be detected is still unknown, because deeper soil horizons of our test area showed only uniformly strong compaction with little contrasts. 相似文献
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Asimenia Gavriilidou Thomas Andrew Mackenzie Pilar Snchez Jos Ruben Tormo Colin Ingham Hauke Smidt Detmer Sipkema 《Marine drugs》2021,19(2)
Marine sponges harbor diverse microbial communities that represent a significant source of natural products. In the present study, extracts of 21 sponge-associated bacteria were screened for their antimicrobial and anticancer activity, and their genomes were mined for secondary metabolite biosynthetic gene clusters (BGCs). Phylogenetic analysis assigned the strains to four major phyla in the sponge microbiome, namely Proteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes. Bioassays identified one extract with anti-methicillin-resistant Staphylococcus aureus (MRSA) activity, and more than 70% of the total extracts had a moderate to high cytotoxicity. The most active extracts were derived from the Proteobacteria and Actinobacteria, prominent for producing bioactive substances. The strong bioactivity potential of the aforementioned strains was also evident in the abundance of BGCs, which encoded mainly beta-lactones, bacteriocins, non-ribosomal peptide synthetases (NRPS), terpenes, and siderophores. Gene-trait matching was performed for the most active strains, aiming at linking their biosynthetic potential with the experimental results. Genetic associations were established for the anti-MRSA and cytotoxic phenotypes based on the similarity of the detected BGCs with BGCs encoding natural products with known bioactivity. Overall, our study highlights the significance of combining in vitro and in silico approaches in the search of novel natural products of pharmaceutical interest. 相似文献