Recruitment of glycosyl hydrolase proteins in a cone snail venomous arsenal: further insights into biomolecular features of Conus venoms     

Violette A  Leonardi A  Piquemal D  Terrat Y  Biass D  Dutertre S  Noguier F  Ducancel F  Stöcklin R  Križaj I  Favreau P 《Marine drugs》2012,10(2):258-280

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Further Investigation of the Mediterranean Sponge Axinella polypoides: Isolation of a New Cyclonucleoside and a New Betaine     

Marialuisa Menna  Anna Aiello  Filomena D��Aniello  Ernesto Fattorusso  Concetta Imperatore  Paolo Luciano  Rocco Vitalone 《Marine drugs》2012,10(11):2509-2518

An exhaustive exploration into the metabolic content of the Mediterranean sponge Axinella-polypoides resulted in the isolation of the new betaine 5 and the new cyclonucleoside 8. The structures of the new metabolites were elucidated by spectroscopic methods assisted by computational methods. The analysis also provided evidence that the sponge does not elaborate pyrrole-imidazole alkaloids (PIAs) but, interestingly, it was shown to contain two already known cyclodipeptides, compounds 9 (verpacamide A) and 10.  相似文献   

Biosynthetic Studies on Water-Soluble Derivative 5c (DTX5c)     

Tamara S. Vilches  Manuel Norte  Antonio Hern��ndez Daranas  Jos�� J. Fern��ndez 《Marine drugs》2012,10(10):2234-2245

The dinoflagellate Prorocentrum belizeanum is responsible for the production of several toxins involved in the red tide phenomenon known as Diarrhetic Shellfish Poisoning (DSP). In this paper we report on the biosynthetic origin of an okadaic acid water-soluble ester derivative, DTX5c, on the basis of the spectroscopical analysis of ¹³C enriched samples obtained by addition of labelled sodium [l-¹³C], [2-¹³C] acetate to artificial cultures of this dinoflagellate.  相似文献   

Characterization of Isoforms of the Lectin Isolated from the Red Algae Bryothamnion seaforthii and Its Pro-Healing Effect     

Luiz Gonzaga do Nascimento-Neto  Romulo Farias Carneiro  Suzete Roberta da Silva  Bruno Rocha da Silva  Francisco Vassiliepe Sousa Arruda  Victor Alves Carneiro  Kyria Santiago do Nascimento  Silvana Saker-Sampaio  Valdemiro Amaro da Silva Jr.  Ana L��cia Figueiredo Porto  Benildo Sousa Cavada  Alexandre Holanda Sampaio  Edson Holanda Teixeira  Celso Shiniti Nagano 《Marine drugs》2012,10(9):1936-1954

Lectins are a structurally heterogeneous group of proteins that have specific binding sites for carbohydrates and glycoconjugates. Because of their biotechnological potential, lectins are widely used in biomedical research. The present study aimed to evaluate the healing potential of the lectin isolated from the marine red alga Bryothamnion seaforthii (BSL). The lectin was purified using ion exchange chromatography with DEAE cellulose and characterized using tandem mass spectrometry. For healing tests, skin wounds were induced in the dorsal thoracic region of mice. These animals were randomly divided into three groups and subjected to topical treatment for 12 days with BSL, bovine serum albumin and 150 mM NaCl. To evaluate the potential of each treatment, the animals were anesthetized and sacrificed on days 2, 7 and 12, respectively. The parameters evaluated included the wound area, the proportion of wound closure and the histological diagnosis. The wound closure was more effective with BSL (Postoperative Day 7 and 12) than controls. The luminal epithelium was completely restructured; the presence of collagen in the dermis and the strongly active presence of young skin annexes demonstrate the potential of treatment with BSL compared with controls. Our findings suggest that BSL has pro-healing properties and can be a potential medical process in the treatment of acute wounds.  相似文献   

Efficient Heterologous Transformation of Chlamydomonas reinhardtii npq2 Mutant with the Zeaxanthin Epoxidase Gene Isolated and Characterized from Chlorella zofingiensis     

Inmaculada Couso  Baldo F. Cordero  Mar��a ��ngeles Vargas  Herminia Rodr��guez 《Marine drugs》2012,10(9):1955-1976

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Contrasting globulin and cysteine proteinase gene expression patterns reveal fundamental developmental differences between zygotic and somatic embryos of oil palm   总被引：2，自引：0，他引：2  

Aberlenc-Bertossi F  Chabrillange N  Duval Y  Tregear J 《Tree physiology》2008,28(8):1157-1167

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A flexible regression model for diameter prediction   总被引：2，自引：2，他引：0  

Edgar Kublin  Nicole Helene Augustin  Juha Lappi 《European Journal of Forest Research》2008,127(5):415-428

We present a functional regression model for diameter prediction. Usually stem form is estimated from a regression model using dbh and height of the sample tree as predictor. With our model additional diameter observations measured at arbitrary locations within the sample tree can be incorporated in the estimation in order to calibrate a standard prediction based on dbh and height. For this purpose, the stem form of a sample tree is modelled as a smooth random function. The observed diameters are assumed as independent realizations from a sample of possible trajectories of the stem contour. The population average of the stem form within a given dbh and height class is estimated with the taper curves applied in the national forest inventory in Germany. Tree deviation from the population average is modelled with the help of a Karhunen–Loève expansion for the random part of the trajectory. Eigenfunctions and scores of the Karhunen–Loève expansion are estimated through conditional expectations within the methodological framework of functional principal component analysis (FPCA). In addition to a calibrated estimation of the stem form, FPCA provides asymptotic pointwise or simultaneous confidence intervals for the calibrated diameter predictions. For the application of functional principal component analysis modelling the covariance function of the random process is crucial. The main features of the functional regression model are discussed informally and demonstrated by means of practical examples.  相似文献   

Environment-friendly short-term protection of palm wood against mould and rot fungi     

Mohsen Bahmani  Leila Fathi  Arno Frühwald 《Wood material science & engineering》2016,11(4):239-247

Felled palm trunks are susceptible to fungi as long as their moisture content is above fibre saturation. During this period, palm wood has to be protected against mould and rot fungi. The study was aimed at testing environment-friendly organic acids for their protecting efficiency. Small samples of date palm (Phoenix dactylifera) and oil palm (Elaeis guineensis) wood were treated with weak organic acids and subsequently infected by moulds and wood-decay fungi. Short dipping of the samples in solutions of 5% acetic acid and propionic acid, respectively, protected all samples for two months from colonization by Aspergillus niger, Penicillium sp., Cladosporium sp. and by a natural infection. Boric acid (4%) used in practice for protection was ineffective. Decay tests with the white-rot fungus Pleurotus ostreatus, the brown-rot species Coniophora puteana and the soft-rot fungus Chaetomium globosum showed that both acids prevented most samples from fungal colonization for three weeks and reduced the decay considerably during two months.  相似文献   

In situ and laboratory non-additive litter mixture effect on C dynamics of Sphagnum rubellum and Molinia caerulea litters     

Sébastien Gogo  Fatima Laggoun-Défarge  Fatima Merzouki  Stéphane Mounier  Audrey Guirimand-Dufour  Nevila Jozja  Arnaud Huguet  Frédéric Delarue  Christian Défarge 《Journal of Soils and Sediments》2016,16(1):13-27

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Spatial differences across the French Pyrenees in the use of local habitat by the endangered semi‐aquatic Pyrenean desman (Galemys pyrenaicus)          下载免费PDF全文

Marjorie Biffi  Anaïs Charbonnel  Laëtitia Buisson  Frédéric Blanc  Mélanie Némoz  Pascal Laffaille 《水产资源保护:海洋与淡水生态系统》2016,26(4):761-774