Effect of Cryopreservation on the Sperm DNA Fragmentation Dynamics of the Bottlenose Dolphin (Tursiops truncatus)          下载免费PDF全文

MJ Sánchez‐Calabuig  C López‐Fernández  SD Johnston  D Blyde  J Cooper  K Harrison  J de la Fuente  J Gosálvez 《Reproduction in domestic animals》2015,50(2):227-235

Sperm DNA fragmentation is one of the major causes of infertility; the sperm chromatin dispersion test (SCDt) evaluates this parameter and offers the advantage of species‐specific validated protocol and ease of use under field conditions. The main purpose of this study was to evaluate sperm DNA fragmentation dynamics in both fresh and post‐thaw bottlenose dolphin sperm using the SCDt following different cryopreservation protocols to gain new information about the post‐thaw differential sperm DNA longevity in this species. Fresh and cryopreserved semen samples from five bottlenose dolphins were examined for sperm DNA fragmentation dynamics using the SCDt (Halomax^®). Sperm DNA fragmentation was assessed immediately at collection and following cryopreservation (T0) and then after 0.5, 1, 4, 8, 24, 48 and 72 h incubation at 37°C. Serially collected ejaculates from four dolphins were frozen using different cryopreservation protocols in a TES‐TRIS‐fructose buffer (TTF), an egg‐yolk‐free vegetable lipid LP1 buffer (LP1) and human sperm preservation medium (HSPM). Fresh ejaculated spermatozoa initially showed low levels of DNA fragmentation for up to 48 h. Lower Sperm DNA fragmentation (SDF) was found in the second fresh ejaculate compared to the first when more than one sample was collected on the same day (p < 0.05); this difference was not apparent in any other seminal characteristic. While there was no difference observed in SDF between fresh and frozen–thawed sperm using the different cryopreservation protocols immediately after thawing (T0), frozen–thawed spermatozoa incubated at 37°C showed an increase in the rate of SDF after 24 h. Sperm frozen in the LP1^? buffer had higher levels (p < 0.05) of DNA fragmentation after 24‐ and 48‐h incubation than those frozen in TTF or HSPM. No correlation was found between any seminal characteristic and DNA fragmentation in either fresh and/or frozen–thawed samples.  相似文献   

Differential Rotation and Dynamics of the Solar Interior     

MJ Thompson  J Toomre  ER Anderson  HM Antia  G Berthomieu  D Burtonclay  SM Chitre  J Christensen-Dalsgaard  T Corbard  M DeRosa  CR Genovese  DO Gough  DA Haber  JW Harvey  F Hill  R Howe  SG Korzennik  AG Kosovichev  JW Leibacher  FP Pijpers  J Provost  EJ Rhodes  J Schou  T Sekii  PB Stark  PR Wilson 《Science (New York, N.Y.)》1996,272(5266):1300-1305

Splitting of the sun's global oscillation frequencies by large-scale flows can be used to investigate how rotation varies with radius and latitude within the solar interior. The nearly uninterrupted observations by the Global Oscillation Network Group (GONG) yield oscillation power spectra with high duty cycles and high signal-to-noise ratios. Frequency splittings derived from GONG observations confirm that the variation of rotation rate with latitude seen at the surface carries through much of the convection zone, at the base of which is an adjustment layer leading to latitudinally independent rotation at greater depths. A distinctive shear layer just below the surface is discernible at low to mid-latitudes.  相似文献   

Reduction of Pesticide Residues on Produce by Rinsing     

Krol WJ  Arsenault TL  Pylypiw HM  Incorvia Mattina MJ 《Journal of agricultural and food chemistry》2000,48(11):5788

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Mechanism of the Zonal Displacements of the Pacific Warm Pool: Implications for ENSO   总被引：7，自引：0，他引：7  

J Picaut  M Ioualalen  C Menkes  T Delcroix  MJ McPhaden 《Science (New York, N.Y.)》1996,274(5292):1486-1489

The western equatorial Pacific warm pool is subject to strong east-west migrations on interannual time scales in phase with the Southern Oscillation Index. The dominance of surface zonal advection in this migration is demonstrated with four different current data sets and three ocean models. The eastward advection of warm and less saline water from the western Pacific together with the westward advection of cold and more saline water from the central-eastern Pacific induces a convergence of water masses at the eastern edge of the warm pool and a well-defined salinity front. The location of this convergence is zonally displaced in association with El Nino-La Nina wind-driven surface current variations. These advective processes and water-mass convergences have significant implications for understanding and simulating coupled ocean-atmosphere interactions associated with El Nino-Southern Oscillation (ENSO).  相似文献   

14 EVALUATION OF BACTERIAL CONTAMINATION AFTER UNCOMPLICATED EQUINE CASTRATIONS.     

MJ  Schoonover  HD Moll  CL Crowson  HW Jann 《Veterinary surgery : VS》2003,32(6):601-602

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Hypovitaminosis A in pigs     

MJ CARRIGAN  JRW GLASTONBURY  JV EVERS 《Australian veterinary journal》1988,65(5):158-160

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Seasonality of sarcoptic mange in pigs in South Australia     

PR DAVIES  MJ MOORE  AM POINTON 《Australian veterinary journal》1991,68(12):390-392

Retrospective analysis of records, from July 1987 to June 1991, of 1140 inspections of slaughtered pigs from 154 swine herds in the Pig Health Monitoring Scheme of South Australia was conducted to evaluate patterns of variation in the prevalence and severity of skin lesions typical of sarcoptic mange. An increase in the severity of sarcoptic mange in winter and spring was observed. There was a substantial reduction in the severity of mange over the period of monitoring. These observations support the role of monitoring at slaughter in the management of endemic disease. They provide some basis for refining control programs for mange to improve cost-effectiveness.  相似文献   

A cross-sectional study to show Eperythrozoon ovis infection is prevalent in Western Australian sheep farms   总被引：4，自引：0，他引：4  

MJ KABAY  RB RICHARDS  TE ELLIS 《Australian veterinary journal》1991,68(5):170-173

A serological survey and risk factor study was conducted to estimate the prevalence of Eperythrozoon ovis infection in Western Australian weaner sheep, the prevalence of farms with infected sheep, and to identify factors affecting initiation and maintenance of infection on the farm. The study was conducted on 91 farms, purposively chosen from 41 randomly selected regional shires stratified by sheep number and rainfall zones. Twenty sheep were selected systematically from a mixed-sex flock on each farm and tested for serum antibody to E ovis using an enzyme-linked immunosorbent assay. Information on putative risk factors was collected using an interview questionnaire. Antibody to E ovis was detected in 4.5% of sheep on 47% of the farms sampled. The prevalence of E ovis infection in sheep was estimated at the 95% confidence level to be between 3.6 and 5.5%, and the prevalence of farms with infected sheep was estimated to be between 37.5 and 56.5%. Most farms with serological evidence of infection occurred in the Great Southern agricultural region (79.5%), south-east of Perth through to Albany (latitude 32 to 34 degrees S, longitude 116 to 120 degrees E), and in the Northern region (12.8%) surrounding Geraldton (latitude 29 degrees S, longitude 114 degrees E). There were significantly more farms (P less than 0.05) with evidence of infection in the Great Southern region compared to the Central region between Geraldton and Perth, and on farms in the region south compared to north of latitude 32 degrees S. None of the putative risk factors examined in the questionnaire were associated with serological evidence of infection on the farm.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

Reply     

MJ STUDDERT 《Australian veterinary journal》1994,71(11):387-388

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CD3 SmIglymphoblastic leukaemia in an outbred domestic guinea pig     

MJ DAY  EK BRIGGS 《Australian veterinary journal》1997,75(3):217-218

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