Utility of systemic voriconazole in equine keratomycosis based on pharmacokinetic‐pharmacodynamic analysis of tear fluid following oral administration     

Norihisa Tamura  Atsushi Okano  Taisuke Kuroda  Hidekazu Niwa  Kanichi Kusano  Yoshikazu Matsuda  Kentaro Fukuda  Hiroshi Mita  Shunichi Nagata 《Veterinary ophthalmology》2020,23(4):640-647

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Metabolism of the herbicides chlorotoluron, diuron, linuron, simazine, and atrazine by CYP1A9 and CYP1C1 from Japanese eel (Anguilla japonica)     

Tomohide Uno  Satoru Kaji  Tatsushi Goto  Hiromasa Imaishi  Masahiko Nakamura  Kengo Kanamaru  Hiroshi Yamagata  Yoshio Kaminishi  Takao Itakura 《Pesticide biochemistry and physiology》2011,101(2):93-102

Through the use of a number of bioconversion experiments we demonstrated that P450 proteins (CYP1A9 and CYP1C1) from Japanese eel (Anguilla japonica) metabolized a number of herbicides and the drug phenacetin. We performed bioconversion experiments in which substrates were added directly to incubation medium. The resulting metabolites were extracted and analyzed by high-performance liquid chromatography. Proteins CYP1A9 and CYP1C1 metabolized 50 nmol of the drug phenacetin to yield 12.1 and 1.1 nmol of product (acetaminophen), respectively. Further incubation of CYP1A9 with 50 nmol of the herbicides chlorotoluron, diuron, linuron, simazine, or atrazine yielded 16.5, 18.5, 7.3, 1.6, or 0.8 nmol of product, respectively. CYP1C1 also metabolized linuron, diuron, and simazine yield 5.4, 4.6, or 0.7 nmol of product, respectively. Next, polyclonal antibody was isolated by immunizing with two conjugated-peptides (amino acid residues 272–290 and 294–310) of CYP1A9. This antibody did not recognize human CYP1A2 or CYP1C1. Western blotting using the antibody revealed one band in the livers of Japanese eel and tilapia (Oreochromis niloticus). Theses results suggest that CYP1A9 and CYP1C1 metabolize herbicides, and that CYP1A9 is an useful biomarker of contamination when detected with this antibody.  相似文献   

On the number and size of cross combinations in a breeding programme of self-fertilizing crops     

K. Yonezawa  H. Yamagata 《Euphytica》1978,27(1):113-116

Summary The number and size of crosses in a breeding programme were discussed to the conclusion that the number of crosses rather than the size of a cross should be increased with a given total population size. The advantage due to this manipulation, however, is relatively small when such genotypes as improved for many (twenty-five or more) loci are to be obtained, or when a high proportion (around 0.1 or more) of available combinations have the potentiality of releasing the objective genotypes. In such a case, the ease with which crossing and cultivation can be carried out becomes the decisive factor.  相似文献   

Neosporosis in a dog: the first case report in Japan.     

T Umemura  K Shiraki  T Morita  A Shimada  M Haritani  M Kobayashi  S Yamagata 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(1):157-159

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Binocular Dissecting Microscopic Studies on the Density of Toruli Tactile in the La Mancha Goat   总被引：1，自引：0，他引：1  

Hidekazu  Wakuri  Michael J.  Guinan Ralph L.  Kitchell 《Anatomia, histologia, embryologia》1990,19(3):255-263

The Merkel cells and associated nervous terminal plates have been called toruli tactiles (TT). These structures can be ubiquitously seen on the shaved hairy skin after staining with a methylene blue solution. Four La Mancha goats of both sexes were examined for the density of TT. TT were visually located under a binocular dissecting microscopic (14x) and then the number of TT in the field was directly counted. The density of TT in different corporal areas of the body surface was estimated from the mean of several two-cm square samples of skin. The results obtained are as follows: The mean number per two-cm square on the whole body surface ranged from 10.06-11.36 in the male specimen and from 12.98-14.32 in the female specimen. The density of TT was rather high in the temporal and buccal areas, on the neck, chin and cranial breast, and on the lateral surface of the arm, thigh and leg. Density was moderate in the fronto-parieto-occipital area (female), on the loin and buttocks, in the upper costal, lower caudo-costal, xiphoid-umbilical and umbilical-pubic areas, and on the lateral surface of the forearm. TT were absent or very few in number on the nose and chin, the scrotum, caudal-pubic area of males, the udder, the vulva, and the digitorum manus et pedis.  相似文献   

Development of loop-mediated isothermal amplification methods for detecting Taylorella equigenitalis and Taylorella asinigenitalis     

Yuta KINOSHITA  Hidekazu NIWA  Yoshinari KATAYAMA  Kazuhisa HARIU 《Journal of Equine Science》2015,26(1):25-29

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A clinical case of equine fungal placentitis with reference to hormone profiles and ultrasonography     

Harutaka MURASE  Hidekazu NIWA  Yoshinari KATAYAMA  Fumio SATO  Tetsuro HADA  Yasuo NAMBO 《Journal of Equine Science》2015,26(4):129-133

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Fucose-containing sulfated polysaccharides from brown seaweeds inhibit proliferation of melanoma cells and induce apoptosis by activation of caspase-3 in vitro     

Ale MT  Maruyama H  Tamauchi H  Mikkelsen JD  Meyer AS 《Marine drugs》2011,9(12):2605-2621

Fucose-containing sulfated polysaccharides (FCSPs) extracted from seaweeds, especially brown macro-algae, are known to possess essential bioactive properties, notably growth inhibitory effects on tumor cells. In this work, we conducted a series of in vitro studies to examine the influence of FCSPs products from Sargassumhenslowianum C. Agardh (FSAR) and Fucus vesiculosus (FVES), respectively, on proliferation of melanoma B16 cells and to investigate the underlying apoptosis promoting mechanisms. Cell viability analysis showed that both FCSPs products, i.e., FSAR and FVES, decreased the proliferation of the melanoma cells in a dose-response fashion, with FSAR being more potent at lower dosages, and FVES being relatively more anti-proliferative than FSAR at higher dosages. Flow cytometric analysis by Annexin V staining of the melanoma cells exposed to the FCSPs products confirmed that both FSAR and FVES induced apoptosis. The FCSPs-induced apoptosis was evidenced by loss of plasma membrane asymmetry and translocation of the cell membrane phospholipids and was accompanied by the activation of caspase-3. The FCSPs bioactivity is proposed to be attributable to distinct structural features of the FCSPs, particularly the presence of sulfated galactofucans (notably in S.henslowianum) and sulfated fucans (notably in F. vesiculosus). This study thus indicates that unfractionated FCSPs may exert bioactive effects on skin cancer cells via induction of apoptosis through cascades of reactions that involve activation of caspase-3.  相似文献   

Ability of salt glands in Rhodes grass (Chloris gayana Kunth) to secrete Na⁺ and K⁺     

Hidekazu KOBAYASHI  Yoshikuni MASAOKA  Yoshitaka TAKAHASHI  Yasuyuki IDE  Setsuro SATO 《Soil Science and Plant Nutrition》2007,53(6):764-771

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Chemical forms and stability of selenium in soil     

Hidekazu Yamada  Yumei Kang  Tomonori Aso  Hiroshi Uesugi  Tomokazu Fujimura  Koyo Yonebayashi 《Soil Science and Plant Nutrition》2013,59(3):385-391

The chemical forms of selenium (Se) in soils derived from different parent rocks, and the stability of Se species in soils were investigated. Around 40% of the total Se occurred as organic Se on the average. Total selenite content was higher than that of total selenate. The content of organic Se decreased with heating of the soils at high temperatures, and most of it disappeared at heating temperatures above 500°C. However, the results of incubation experiments indicated that Se species were stable in soil, and that their chemical forms did not change appreciably with the variation of soil conditions. In soluble Se, organic Se accounted for about 50%, and in contrast to the total Se, the selenate content was higher than that of selenite.  相似文献