SuperSAGE revealed different classes of early resistance response genes in Capsicum chinense plants harboring L ³ -resistance gene infected with Pepper mild mottle virus     

Hiroyuki Hamada  Hideo Matsumura  Reiko Tomita  Ryohei Terauchi  Kazumi Suzuki  Kappei Kobayashi 《Journal of General Plant Pathology》2008,74(4):313-321

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Effects of cooling rate on post-thaw motility and fertility of Japanese pearl oyster <Emphasis Type="Italic">Pinctada fucata martensii</Emphasis> spermatozoa     

Kahori Arita  Kiyoshi Isowa  Takashi Ishikawa  Hideo Aoki  Hiromi Ohta 《Fisheries Science》2012,78(3):625-630

The post-thaw motility and fertility of Japanese pearl oyster sperm show large variances, even among sperm samples obtained from the same individuals. This study aimed to clarify the factors that cause such differences. Spermatozoa were diluted 50 times with diluent comprising 10 % methanol, 18 % fetal bovine serum, and 72 % seawater, and dispensed into 0.25 ml straws. A total of 59 straws were cooled, one by one, at 11 different heights from the surface of liquid nitrogen (LN) to −50 °C, and then immediately immersed in LN. After thawing the straws, the relationships between the cooling rate and the post-thaw motility and post-thaw fertility of the spermatozoa were examined. Both the post-thaw motility and the post-thaw fertility showed a sharp peak when the straws were cooled at around −20 °C/min. There was a strong correlation between post-thaw motility and fertility (P < 0.001). There was a large difference in the cooling rates and the post-thaw motilities and fertilities of the spermatozoa, even between straws cooled at the same height. These results indicate that the optimum range for the cooling rate of oyster spermatozoa is quite narrow, and the method of cooling straws at a fixed distance from the LN surface is unsuitable for the cryopreservation of Japanese pearl oyster spermatozoa.  相似文献   

Correction to: Extractives of<Emphasis Type="Italic"> Quercus crispula</Emphasis> sapwood infected by the pathogenic fungi<Emphasis Type="Italic"> Raffaelea quercivora</Emphasis> I: comparison of sapwood extractives from noninfected and infected samples     

Kayoko Imai  Tohru Mitsunaga  Hiroyuki Takemoto  Toshihiro Yamada  Shin-ichiro Ito  Hideo Ohashi 《Journal of Wood Science》2018,64(4):463-463

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Genetic background of an invasive Lolium population in central Japan using chloroplast DNA and SSR markers     

S KUROKAWA  H KOBAYASHI  K IKEDA 《Weed Research》2010,50(3):245-252

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Strength properties of laminated veneer lumber in compression perpendicular to its grain     

Hirofumi Ido  Hirofumi Nagao  Hideo Kato  Atsushi Miyatake  Yasushi Hiramatsu 《Journal of Wood Science》2010,56(5):422-428

Tests of compression perpendicular to the grain were carried out on laminated veneer lumber (LVL) and timber. The species tested were sugi, radiata pine, karamatsu, akamatsu, and dahurian larch; two sets of sugi specimens were tested, with the sugi LVL products being manufactured in different plants. The strength properties of the materials for different loading directions were compared for LVL and timber. At 5% compressive strain in the same materials, the average stress in the tangential direction of timber was larger than that in the radial direction for all species except for radiata pine, and the average stress in the edge-wise direction of LVL was larger than that in the flat-wise direction for all species except for radiata pine. When the stress at 5% strain was compared in the same direction, the average stress of LVL in the edge-wise direction was larger than that in timber in the tangential direction for all species, but there were no great differences between the average stress of LVL in the fl at-wise direction and that of timber in the radial direction for all species except for radiation pine. There was a close relationship between density and stress at 5% strain in LVL, especially in the edge-wise direction. For all results, radiata pine did not follow the trend of the other species; The large annual ring width of radiata pine was considered to have affected the results.  相似文献   

Induction of apoptotic lesions in liver and lymphoid tissues and modulation of cytokine mRNA expression by acute exposure to deoxynivalenol in piglets     

Osamu Mikami  Hiroyuki Yamaguchi  Hideo Murata  Yasuyuki Nakajima  Shigeru Miyazaki 《Journal of veterinary science (Suw?n-si, Korea)》2010,11(2):107-113

Six 1-month-old piglets were intravenously injected with deoxynivalenol (DON) at the concentration of 1 mg/kg body weight, with three pigs each necropsied at 6 and 24 h post-injection (PI) for investigation of hepatotoxicity and immunotoxicity with special attention to apoptotic changes and cytokine mRNA expression. Histopathological examination of the DON-injected pigs revealed systemic apoptosis of lymphocytes in lymphoid tissues and hepatocytes. Apoptosis of lymphocytes and hepatocytes was confirmed by the TdT-mediated dUTP-biotin nick end-labeling (TUNEL) method and immunohistochemical staining against single-stranded DNA and cleaved caspase-3. The number of TUNEL-positive cells in the thymus and Peyer''s patches of the ileum was increased at 24 h PI compared to 6 h PI, but the peak was at 6 h PI in the liver. The mRNA expression of interleukin (IL)-1β, IL-6, IL-18, and tumor necrosis factor (TNF)-α in the spleen, thymus and mesenteric lymph nodes were determined by semi-quantitative RT-PCR, and elevated expression of IL-1β mRNA at 6 h PI and a decrease of IL-18 mRNA at 24 h PI were observed in the spleen. IL-1β and IL-6 mRNA expressions increased significantly at 6 h PI in the thymus, but TNF-α decreased at 6 h PI in the mesenteric lymph nodes. These results show the apoptosis of hepatocytes suggesting the hepatotoxic potential of DON, in addition to an immunotoxic effect on the modulation of proinflammatory cytokine genes in lymphoid organs with extensive apoptosis of lymphocytes induced by acute exposure to DON in pigs.  相似文献   

Growth variability of Pacific saury Cololabis saira larvae under contrasting environments across the Kuroshio axis: survival potential of minority versus majority          下载免费PDF全文

Akinori Takasuka  Kirara Nishikawa  Hiroshi Kuroda  Takeshi Okunishi  Yugo Shimizu  Hideo Sakaji  Shin‐Ichi Ito  Tadashi Tokai  Yoshioki Oozeki 《Fisheries Oceanography》2016,25(4):390-406

Growth variability was examined for Pacific saury Cololabis saira larvae under contrasting environments across the Kuroshio axis, based on samples collected during the winter spawning season in 2013 and 2014. The growth rate index (residual of the otolith marginal 3‐day mean increment width from the linear regression on knob length) of larvae was compared among three areas: the inshore side of the Kuroshio axis, the Kuroshio axis, and the offshore side of the Kuroshio axis in relation to sea surface temperature (SST), salinity (SSS) and chlorophyll‐a (CHL) concentration. The larvae were more densely distributed in the Kuroshio axis and offshore areas of higher temperature and salinity and lower chlorophyll‐a concentration than in the inshore areas of lower temperature and salinity and higher chlorophyll‐a concentration. No marked differences in the growth rate index were found among the three areas, even though the larvae in the inshore areas showed slightly higher growth rates in 2013. Despite the broad ranges of environmental factors, no clear relationship between the growth rate index and any environmental factor was detected. The survival potential of Pacific saury larvae was considered to be at least comparable under contrasting environments across the Kuroshio axis. Such a geographical homogeneity is concluded to be attributable to compensable effects of physical and biological factors. We hypothesize that the minority under physically‐unfavorable but biologically‐favorable conditions on the inshore side of the Kuroshio axis could survive equally well as the majority under physically‐favorable but biologically‐unfavorable conditions around the Kuroshio axis and on the offshore side of the Kuroshio axis.  相似文献   

Runoff water quality characteristics in a small agriculture watershed     

Hideo?NakasoneEmail author 《Paddy and Water Environment》2003,1(4):183-188

I have observed the water quality in the Ohwada District of Kasumigaura Town near Lake Kasumigaura for a period of over seven years (1993–1999). During this observation period, sampling was performed for 3 years at 4-h intervals using an automatic sampler, and the water quality was subsequently analyzed. As a result of this long-term precision study of water quality fluctuation, important new information was obtained. First, the quantity of nitrogen found to have been removed was great, especially in the paddy fields, and this contributed to water quality purification. Secondly, the paddy fields produced algae and this algae supported denitrification in the paddy fields. However, the paddy fields increased the COD runoff. Thirdly, for determining the average water quality by monitoring, monthly sampling is sufficient, though for understanding the fluctuation pattern of the water quality, weekly sampling is necessary.  相似文献   

Development and validation of a sandwich ELISA for use in measuring concentrations of canine surfactant protein A in serum of dogs     

Sone K  Akiyoshi H  Aoki M  Sugii S  Ohashi F 《American journal of veterinary research》2011,72(6):833-837

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Effect of growth on monocarboxylate transporters and indicators of energy metabolism in the gluteus medius muscle of Thoroughbreds     

Kitaoka Y  Hoshino D  Mukai K  Hiraga A  Takemasa T  Hatta H 《American journal of veterinary research》2011,72(8):1107-1111

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