Oestrogen and Progesterone Receptor Gene Expression in Canine Oocytes and Cumulus Cells Throughout the Oestrous Cycle     

JSA Gonçalves  CI Vannucchi  FC Braga  FF Paula-Lopes  MP Milazzotto  MEOA Assumpção  JA Visintin 《Reproduction in domestic animals》2009,44(S2):239-242

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Quantitation of house dust mites and house dust mite allergens in the microenvironment of dogs     

Randall A  Hillier A  Cole LK  Kwochka KW  Needham G  Wassom DL 《American journal of veterinary research》2003,64(10):1301-1309

OBJECTIVE: To quantitate the density of Dermatophagoides farinae and D pteronyssinus and concentrations of house dust mite (HDM) allergens (Der f 1, Der p 1, and Group 2 allergens) in the indoor microenvironment of dogs. Sample Population-50 homes in Columbus, Ohio. PROCEDURES: n each home, samples of dust were collected from 3 locations in which dogs spent most time. Whenever possible, the species of mites collected was identified. Mite density (mites/g of dust) was assessed, and allergen concentrations were assayed by standardized ELISAs. Relative humidity and temperature in each home were monitored during a 5-day period. Characteristics of homes and sample sources were evaluated. RESULTS: Dust samples from all 50 homes contained > or = 1 HDM allergen; Der f 1 and Der p 1 were detected in 100 and 74% of homes, respectively. Fifteen homes had HDMs; compared with D pteronyssinus, D farinae was found more commonly (14/15 homes) and at a higher density. Basements, homes without central air-conditioning, and dog beds that were > or = 1 year old had high HDM allergen concentrations. Homes with > or = 2 microg of Der f 1 or Group 2 allergens/g of dust or > or = 100 mites/g of dust were significantly more likely to have a maximum relative humidity > or = 75%. CONCLUSIONS AND CLINICAL RELEVANCE: Results indicate the presence of HDMs and HDM allergens in the specific microenvironment of dogs in homes. Factors associated with high levels of exposure were identified, which may be associated with increased risk for sensitization and development of atopic diseases.  相似文献   

What constitutes freedom from disease in livestock?     

FC BALDOCK 《Australian veterinary journal》1998,76(8):544-545

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Intradermal testing in healthy horses and horses with chronic obstructive pulmonary disease, recurrent urticaria, or allergic dermatitis.     

E Jose-Cunilleras  C W Kohn  A Hillier  W J Saville  G Lorch 《Journal of the American Veterinary Medical Association》2001,219(8):1115-1121

OBJECTIVE: To compare responses to a variety of intradermally injected allergens among healthy horses and horses with chronic obstructive pulmonary disease (COPD), recurrent urticaria (RU), and atopic dermatitis-insect hypersensitivity (allergic dermatitis [AD]). DESIGN: Case-control study. ANIMALS: 86 horses. PROCEDURE: Results of intradermal testing for horses with COPD, RU, or AD were compared with results for healthy horses. RESULTS: Compared with healthy horses, horses with COPD, RU, and AD were significantly more likely to have positive (> or = 3+) reactions to intradermal allergens (molds, weeds, trees, grasses-crops, and insects) 30 minutes (immediate reaction), 4 hours (late-phase reactions), and 24 hours (delayed-phase reactions) after exposure. In addition, diseased horses reacted to a significantly higher number of allergens in each allergen group than did healthy horses. CONCLUSIONS AND CLINICAL RELEVANCE: Reactions to individual allergens should not be used to determine that horses have hypersensitivity. Overall patterns of reactivity to intradermal allergens may be helpful in management when used in conjunction with a compatible history and evidence of potential exposure to allergens in horses with conditions associated with hypersensitivity to environmental allergens.  相似文献   

A comparison of the ovulation rate, fertilization rate and embryo survival of hand-mated and lot-mated gilts     

T D Rich  E J Turman  J C Hillier 《Journal of animal science》1968,27(2):443-446

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The ACVD task force on canine atopic dermatitis (XVI): laboratory evaluation of dogs with atopic dermatitis with serum-based "allergy" tests.     

D J DeBoer  A Hillier 《Veterinary immunology and immunopathology》2001,81(3-4):277-287

Serum-based in vitro "allergy tests" are commercially available to veterinarians, and are widely used in diagnostic evaluation of a canine atopic patient. Following initial clinical diagnosis, panels of allergen-specific IgE measurements may be performed in an attempt to identify to which allergens the atopic dog is hypersensitive. Methodology for these tests varies by laboratory; few critical studies have evaluated performance of these tests, and current inter-laboratory standardization and quality control measures are inadequate. Other areas where information is critically limited include the usefulness of these tests in diagnosis of food allergy, the effect of extrinsic factors such as season of the year on results, and the influence of corticosteroid treatment on test results. Allergen-specific IgE serological tests are never completely sensitive, nor completely specific. There is only partial correlation between the serum tests and intradermal testing; however, the significance of discrepant results is unknown and unstudied. Variation in test methodologies along with the absence of universal standardization and reporting procedures have created confusion, varying study results, and an inability to compare between studies performed by different investigators.  相似文献   

Potential of bumble bees as bio-vectors of Clonostachys rosea for Botrytis blight management in lowbush blueberry     

K. W. Reeh  N. K. Hillier  G. C. Cutler 《Journal of pest science》2014,87(3):543-550

The effectiveness of bees as vectors for microbial biological control agents has been demonstrated in greenhouses and some small-acreage crops. Less is known about the viability of this tactic for other cropping systems, or the effects of outfitting hives with dispensers on the bees. Experiments conducted in lowbush blueberry (Vaccinium angustifolium) fields indicated that home-made wooden, Koppert, and Enviroquest dispensers had no significant effects on foraging behavior or pollen loads of the bumble bee Bombus impatiens, but the presence of a dispenser did reduce the rate of workers exiting hives. Following exit of bees from dispensers, no difference was found in the quantity of the microbial agent Clonostachys rosea applied to the bees or blossoms, and distribution of product in the field was not influenced by dispenser design or distance from hives. However, the prevalence of the pathogen Botrytis cinerea in blossoms from cages that contained hives with dispensers with C. rosea was significantly less (10–20 %) than blossom infection in control plots, despite unseasonably inclement conditions and restricted foraging. These results suggest that C. rosea can be effectively delivered by commercial bumble bees for prevention of B. cinerea infection in V. angustifolium, but the efficacy of the technique will be limited during periods of poor weather that often occur in regions where lowbush blueberries are grown. Some technical issues with dispensers and commercial B. impatiens hives also need to be addressed before widespread use can be expected within commercial lowbush blueberry production.  相似文献   

Parameters of the Reproductive Tract,Spermatogenesis, Daily Sperm Production and Major Seminal Plasma Proteins of Tropically Adapted Morada Nova Rams     

FML Sousa  CH Lobo  ESB Menezes  JPA Rego  RV Oliveira  AC Lima‐Souza  M Fioramonte  FC Gozzo  RCFF Pompeu  MJD Cândido  JT Oliveira  AA Moura 《Reproduction in domestic animals》2014,49(3):409-419

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The development of rare earth element-labelled potassium-depleted clays for use as cohesive sediment tracers in aquatic environments   总被引：2，自引：1，他引：1  

Kate L. Spencer  Keiko Suzuki  Stephen Hillier 《Journal of Soils and Sediments》2011,11(6):1052-1061

Purpose

Understanding the transport behaviour of fine cohesive sediment is fundamental to the sustainable management of aquatic environments. Sediment tracing techniques are widely used for measuring the transport pathways of sand-sized material in the field. However, the development of tracers, including geochemically labelled clays, for fine, cohesive sediment is more problematic. Such tracers should have chemical signatures that can be easily detected following significant dilution in the field and should remain constant for the duration of the tracer study.

Materials and methods

We have examined the potential of rare earth element (REE)-labelled phlogopite and hydrobiotite as cohesive sediment tracers. Clays were first treated with sodium tetraphenylborate to extract interlayer potassium and enhance their cation exchange capacity. Ho, La and Sm were then sorbed to the clays in batch experiments. Desorption of the chemical signature in both fresh and saline conditions was examined after 1 and 10 days.

Results and discussion

Potassium extraction enhanced REE sorption, resulting in REE concentrations in excess of 40,000 mg kg^?1 in the labelled clays, and these signatures should be easily detected following dilution in the aquatic environment. In both fresh and saline conditions, over 90% of the tracer signature was retained over a 1-day period. However, over longer time scales, there was considerable loss of the REE signature.

Conclusions

Over short time scales, there is considerable potential to use these materials as cohesive sediment tracers. Over longer time scales, although much of the label is lost, the tracers could still provide qualitative information identifying net sediment transport pathways.  相似文献   

The minor role of pigs in outbreaks of foot-and-mouth disease of northern Thailand     

P CHAMNANPOOD  PC CLELAND†  FC BALDOCK‡  LJ GLEESONt† § 《Australian veterinary journal》1995,72(4):142-144

SUMMARY: A study was undertaken in northern Thailand to examine the involvement of pigs in outbreaks of foot-and-mouth disease (FMD). Data were collected by surveying selected villages, by serological monitoring of pigs and by investigating outbreaks. Fifty-three of 58 villages (91%) surveyed reported that pigs did not develop FMD during the most recent outbreak. The source of 49/60 (82%) outbreaks was attributed to either recent purchases of infected cattle and buffalo or commingling of cattle and buffalo with stock from an infected neighbouring village. One of 60 villages (1.7%) reported that the source was introduced infected pigs. There was no association between the various hypothesised risk factors relating to the management of pigs and the frequency of FMD outbreaks in the survey. The percentage of seropositive pigs during 3 rounds of serological monitoring conducted at 6-monthly intervals in selected villages was 3.5%, 2.6% and 0%, respectively. No clinically affected pigs were observed in 11 outbreak investigations. It was concluded that pigs did not commonly become infected when there were outbreaks of FMD in village cattle and buffalo in northern Thailand. This was probably due to the pig feeding and housing practices employed by villagers that protected pigs from exposure to virus from infected cattle or buffalo, or their products.  相似文献